pycom06g13160

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010013
Physical Location & Seq
Forward (+)
18219492 .. 18223163
3672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13160.1

Sequence Viewer

Length: 1248 bp
ATGGAGGTGGAACTGAAGGAGATGCTGAACGATCTGGAATCGCTGAAGAGATCGCTGGCCGATCCTTCTCATCATGCTCCAATCGATAAGCTACAATTGCGCGTAGAACGCCTTACGAGCCTTTCAAAGTCTGGACCTGTCCGGCGTTCAAAAGTGAAGGATATGAGCGCCGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGTATTGTGGAGAACTATGAAAGAATACGGGATTTCTCAGTTGCCATTGTTGGAGTAGGCGGTGTAGGAAGTGTAGCAGCTGAGATGCTTACAAGATGTGGTATTGGTCGCCTTTTGTTGTATGATTATGACAAAGTAGAGTTAGCTAACATGAATAGGTTATTCTTTCGACCAGAACAGAGTGGCATGACAAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCCGATGTTATACTCGAGAGCTATACACTGAACATCACAACAGTGCAAGGTTTTGATACCTTTATGTCTAGTTTAAAAAATAAATCGTTTCGCCCAAATAAAGAAGGCACTGGAATAGACCTTGTGTTAAGCTGTGTTGATAATTATGAAGCAAGAATGGCTGTTAATCAGGCTTGCAATGAACTGAATCAGACATGGATGGAGTCTGGTGTGTCTGAGGATGCCGTTTCAGGCCATATTCAGTTGCTGATTCCTGGTGAAACTGCATCATCTGGAGTGGATGAACGTACACTTAAGCGTGAAGGGGTTTGTGCTGCATCTTTACCTACCACTATGGGAGTTGTTGCTGGGCTTCTAGTCCAAAATACACTAAAATACTTGCTGAACTTCGGAAACGTCTCCCCATACTTGGGATATAATTCTCTTAAAGACTTCTTCCCAACCATGGAAATGAAGCCAAACCCTCAGTGTTCTAATTTTGCTTGTCTGGAACGGCAGAAAGAATACTTGCTTGTAAAGCCAGCTAGGGATGCTGCAATTAAAGCAAAGATGGAAGCTGAAGCATCATCAGTTCCGGAATCTCCACTTCATAGTGATAATGAATGGGAAATAAGTGTTGTTGATGATACCGAGCCGGAAAGTACAGATGCCAAAACTTCAGATGCACTTCCAGAAGGTCTTGTTCGCGAGCTCCCCAGTGCAGATGAGTTCCAAAAACCCTCAACTGAAGCTACAGAGGGTACTATCGACGACCTAGAAGATCTTCAAAAGCAACTTGAGGCCCTAAATTCTTGA

Protein Analysis

416

Amino Acids

45.78

Weight (kDa)

4.7

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 309 2.3e-51 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 102, 1140
AccIII TCCGGA 1 cut(s) 1027
AciI CCGC 1 cut(s) 282
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 1240
AcuI CTGAAG 6 cut(s) 35, 65, 197, 1032, 1095, 1200
AfaI GTAC 4 cut(s) 432, 742, 1096, 1195
AfiI CCNNNNNNNGG 3 cut(s) 862, 863, 898
AflII CTTAAG 1 cut(s) 746
AgsI TTSAA 3 cut(s) 126, 150, 1220
AjnI CCWGG 1 cut(s) 706
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 9 cut(s) 91, 302, 368, 474, 585, 977, 1010, 1144, 1184
AluI AGCT 9 cut(s) 91, 302, 368, 474, 585, 977, 1010, 1144, 1184
Alw21I GWGCWC 1 cut(s) 1146
Alw26I GTCTC 1 cut(s) 856
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 2 cut(s) 425, 700
Ama87I CYCGRG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 1027
AoxI GGCC 3 cut(s) 57, 685, 1233
ApeKI GCWGC 4 cut(s) 299, 425, 767, 986
ApoI RAATTY 1 cut(s) 1240
Asp700I GAANNNNTTC 1 cut(s) 1215
AspLEI GCGC 2 cut(s) 102, 170
AspS9I GGNCC 2 cut(s) 134, 1234
AsuHPI GGTGA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 1146
Bbv12I GWGCWC 1 cut(s) 1146
BbvI GCAGC 4 cut(s) 311, 412, 754, 973
BccI CCATC 3 cut(s) 214, 646, 997
BceAI ACGGC 2 cut(s) 662, 962
BciT130I CCWGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 856
BfaI CTAG 4 cut(s) 522, 809, 978, 1208
BfmI CTRYAG 1 cut(s) 1185
BfoI RGCGCY 1 cut(s) 171
BfrI CTTAAG 1 cut(s) 746
BglII AGATCT 1 cut(s) 1213
BisI GCNGC 4 cut(s) 300, 426, 768, 987
BlsI GCNGC 4 cut(s) 301, 427, 769, 988
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 134, 1234
BmrFI CCNGG 1 cut(s) 708
BmrI ACTGGG 1 cut(s) 1143
BmuI ACTGGG 1 cut(s) 1143
BpmI CTGGAG 1 cut(s) 747
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 171, 897
BsaWI WCCGGW 1 cut(s) 1027
Bsc4I CCNNNNNNNGG 3 cut(s) 862, 863, 898
Bse1I ACTGG 2 cut(s) 568, 1149
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 1027
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 171, 897
BseGI GGATG 5 cut(s) 225, 657, 679, 739, 988
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 3 cut(s) 862, 863, 898
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 4 cut(s) 273, 294, 660, 932
BseNI ACTGG 2 cut(s) 568, 1149
BseXI GCAGC 4 cut(s) 311, 412, 754, 973
BseYI CCCAGC 1 cut(s) 800
BsgI GTGCAG 1 cut(s) 1173
Bsh1236I CGCG 2 cut(s) 102, 1140
BshFI GGCC 3 cut(s) 59, 687, 1235
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 1146
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 3 cut(s) 142, 1028, 1088
BslI CCNNNNNNNGG 3 cut(s) 862, 863, 898
BsmAI GTCTC 1 cut(s) 856
BsmBI CGTCTC 1 cut(s) 856
BsnI GGCC 3 cut(s) 59, 687, 1235
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 1146
Bsp13I TCCGGA 1 cut(s) 1027
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 31, 50, 61, 1213
Bsp19I CCATGG 1 cut(s) 897
Bsp68I TCGCGA 1 cut(s) 1140
BspACI CCGC 1 cut(s) 282
BspANI GGCC 3 cut(s) 59, 687, 1235
BspCNI CTCAG 4 cut(s) 272, 295, 661, 931
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 1027
BspFNI CGCG 2 cut(s) 102, 1140
BspPI GGATC 1 cut(s) 56
BspTI CTTAAG 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 2 cut(s) 568, 1149
BssECI CCNNGG 2 cut(s) 171, 897
BssMI GATC 4 cut(s) 31, 50, 61, 1213
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 197, 496
Bst6I CTCTTC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 57, 628, 975, 1142
BstDEI CTNAG 4 cut(s) 259, 303, 669, 918
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 5 cut(s) 225, 657, 679, 739, 988
BstFNI CGCG 2 cut(s) 102, 1140
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 102, 170
BstKTI GATC 4 cut(s) 34, 53, 64, 1216
BstMAI GTCTC 1 cut(s) 856
BstMBI GATC 4 cut(s) 31, 50, 61, 1213
BstMWI GCNNNNNNNGC 7 cut(s) 97, 108, 117, 611, 970, 983, 995
BstNI CCWGG 1 cut(s) 708
BstSCI CCNGG 1 cut(s) 706
BstSFI CTRYAG 1 cut(s) 1185
BstUI CGCG 2 cut(s) 102, 1140
BstV1I GCAGC 4 cut(s) 311, 412, 754, 973
BstX2I RGATCY 1 cut(s) 1213
BstYI RGATCY 1 cut(s) 1213
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 3 cut(s) 59, 687, 1235
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 897
BtsCI GGATG 5 cut(s) 225, 657, 679, 739, 988
BtsIMutI CAGTG 5 cut(s) 479, 501, 561, 926, 1156
BtuMI TCGCGA 1 cut(s) 1140
Cac8I GCNNGC 4 cut(s) 57, 628, 975, 1142
CaiI CAGNNNCTG 2 cut(s) 425, 700
CfoI GCGC 2 cut(s) 102, 170
Cfr13I GGNCC 2 cut(s) 134, 1234
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 4 cut(s) 431, 741, 1095, 1194
CviAII CATG 5 cut(s) 74, 373, 409, 648, 898
CviQI GTAC 4 cut(s) 431, 741, 1095, 1194
DdeI CTNAG 4 cut(s) 259, 303, 669, 918
DpnI GATC 4 cut(s) 33, 52, 63, 1215
DpnII GATC 4 cut(s) 31, 50, 61, 1213
DraI TTTAAA 1 cut(s) 528
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 1144
Eco130I CCWWGG 1 cut(s) 897
Eco24I GRGCYC 1 cut(s) 1146
Eco47I GGWCC 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 1144
Eco57I CTGAAG 6 cut(s) 35, 65, 197, 1032, 1095, 1200
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 1144
EcoO109I RGGNCCY 1 cut(s) 1234
EcoRII CCWGG 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 897
EcoT38I GRGCYC 1 cut(s) 1146
ErhI CCWWGG 1 cut(s) 897
Esp3I CGTCTC 1 cut(s) 856
FaeI CATG 5 cut(s) 77, 376, 412, 651, 901
FatI CATG 5 cut(s) 73, 372, 408, 647, 897
Fnu4HI GCNGC 4 cut(s) 300, 426, 768, 987
FokI GGATG 5 cut(s) 232, 664, 686, 746, 995
FriOI GRGCYC 1 cut(s) 1146
Fsp4HI GCNGC 4 cut(s) 300, 426, 768, 987
FspBI CTAG 4 cut(s) 522, 809, 978, 1208
GlaI GCGC 2 cut(s) 101, 169
GluI GCNGC 4 cut(s) 300, 426, 768, 987
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 1 cut(s) 747
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 3 cut(s) 59, 687, 1235
HapII CCGG 3 cut(s) 142, 1028, 1088
HhaI GCGC 2 cut(s) 102, 170
Hin1II CATG 5 cut(s) 77, 376, 412, 651, 901
Hin6I GCGC 2 cut(s) 100, 168
HinP1I GCGC 2 cut(s) 100, 168
HinfI GANTC 5 cut(s) 38, 640, 656, 703, 1031
HpaII CCGG 3 cut(s) 142, 1028, 1088
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 1 cut(s) 743
Hpy188I TCNGA 6 cut(s) 216, 445, 645, 670, 845, 1114
Hpy8I GTNNAC 1 cut(s) 743
Hpy99I CGWCG 1 cut(s) 1205
HpyAV CCTTC 6 cut(s) 10, 75, 151, 551, 749, 1121
HpyCH4III ACNGT 2 cut(s) 197, 496
HpyCH4IV ACGT 2 cut(s) 739, 849
HpyCH4V TGCA 7 cut(s) 499, 630, 719, 770, 989, 1118, 1154
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 108, 117, 611, 970, 983, 995
HpyF3I CTNAG 4 cut(s) 259, 303, 669, 918
HpySE526I ACGT 2 cut(s) 739, 849
Hsp92II CATG 5 cut(s) 77, 376, 412, 651, 901
HspAI GCGC 2 cut(s) 100, 168
Kpn2I TCCGGA 1 cut(s) 1027
Kzo9I GATC 4 cut(s) 31, 50, 61, 1213
LmnI GCTCC 2 cut(s) 82, 1149
Lsp1109I GCAGC 4 cut(s) 311, 412, 754, 973
MaeI CTAG 4 cut(s) 522, 809, 978, 1208
MaeII ACGT 2 cut(s) 739, 849
MalI GATC 4 cut(s) 33, 52, 63, 1215
MboI GATC 4 cut(s) 31, 50, 61, 1213
MboII GAAGA 4 cut(s) 58, 880, 1208, 1223
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 1 cut(s) 1213
MhlI GDGCHC 1 cut(s) 1146
MluCI AATT 6 cut(s) 95, 595, 871, 928, 990, 1240
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 7 cut(s) 166, 210, 664, 927, 1183, 1183, 1225
MroI TCCGGA 1 cut(s) 1027
MroXI GAANNNNTTC 1 cut(s) 1215
MseI TTAA 6 cut(s) 527, 581, 618, 747, 879, 993
MslI CAYNNNNRTG 2 cut(s) 494, 902
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 746
MspI CCGG 3 cut(s) 142, 1028, 1088
MspR9I CCNGG 1 cut(s) 708
MunI CAATTG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 708
MvnI CGCG 2 cut(s) 102, 1140
MwoI GCNNNNNNNGC 7 cut(s) 97, 108, 117, 611, 970, 983, 995
NcoI CCATGG 1 cut(s) 897
NdeII GATC 4 cut(s) 31, 50, 61, 1213
NlaIII CATG 5 cut(s) 77, 376, 412, 651, 901
NmeAIII GCCGAG 1 cut(s) 196
NruI TCGCGA 1 cut(s) 1140
OliI CACNNNNGTG 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 1215
PfeI GAWTC 4 cut(s) 38, 640, 703, 1031
PkrI GCNGC 4 cut(s) 301, 427, 769, 988
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp124BI GAGCTC 1 cut(s) 1146
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 2 cut(s) 134, 1234
PstNI CAGNNNCTG 2 cut(s) 425, 700
PsuI RGATCY 1 cut(s) 1213
PvuII CAGCTG 1 cut(s) 302
RruI TCGCGA 1 cut(s) 1140
RsaI GTAC 4 cut(s) 432, 742, 1096, 1195
RsaNI GTAC 4 cut(s) 431, 741, 1095, 1194
RseI CAYNNNNRTG 2 cut(s) 494, 902
SacI GAGCTC 1 cut(s) 1146
SaqAI TTAA 6 cut(s) 527, 581, 618, 747, 879, 993
SatI GCNGC 4 cut(s) 300, 426, 768, 987
Sau3AI GATC 4 cut(s) 31, 50, 61, 1213
Sau96I GGNCC 2 cut(s) 134, 1234
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 1 cut(s) 1146
SfcI CTRYAG 1 cut(s) 1185
Sfr274I CTCGAG 1 cut(s) 467
SinI GGWCC 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 494, 902
SmlI CTYRAG 3 cut(s) 467, 746, 1229
SmoI CTYRAG 3 cut(s) 467, 746, 1229
Sse9I AATT 6 cut(s) 95, 595, 871, 928, 990, 1240
SsiI CCGC 1 cut(s) 282
SspMI CTAG 4 cut(s) 522, 809, 978, 1208
SstI GAGCTC 1 cut(s) 1146
StyD4I CCNGG 1 cut(s) 706
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 2 cut(s) 197, 496
TaiI ACGT 2 cut(s) 742, 852
TaqI TCGA 4 cut(s) 84, 391, 468, 1200
TasI AATT 6 cut(s) 95, 595, 871, 928, 990, 1240
TatI WGTACW 2 cut(s) 430, 1094
TfiI GAWTC 4 cut(s) 38, 640, 703, 1031
Tru1I TTAA 6 cut(s) 527, 581, 618, 747, 879, 993
Tru9I TTAA 6 cut(s) 527, 581, 618, 747, 879, 993
TscAI CASTG 5 cut(s) 486, 501, 568, 926, 1156
TseI GCWGC 4 cut(s) 299, 425, 767, 986
TspDTI ATGAA 8 cut(s) 255, 389, 615, 648, 750, 920, 1031, 1068
TspRI CASTG 5 cut(s) 486, 501, 568, 926, 1156
Vha464I CTTAAG 1 cut(s) 746
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 1240
XhoI CTCGAG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 1215
XspI CTAG 4 cut(s) 522, 809, 978, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.