FvH4_5g08320

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010013
Physical Location & Seq
Reverse (-)
4832764 .. 4837086
4323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08320.t1

Sequence Viewer

Length: 1281 bp
ATGGAGGCGGAGCTGAAGGAGATGCTCAGCGACCTCGAATCGCTGAAGAGATCGCTCACCGATCCTTCTCACACTGCTCCAATCGATAAGCTACAAATGCGTGTTGAGCGCCTTACCAGTCTTGCAAAAACCGGACCTGTTCGGCGCTCCAAAGTTCAGGATATGAGCGCTGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGCATTGTGAAAAACTATGAAAGAATTCGGGAGTTCTCGGTAGCCATAGTTGGAGTAGGTGGTGTAGGAAGTGTAGCAGCTGAGATGCTTACAAGATGTGGTATTGGCCGTCTTCTGTTGTATGATTATGACAAAGTAGAGCTAGCTAACATGAATAGGTTATTCTTTCGTCCAGAGCAGAGTGGTATGACTAAAACAGATGCGGCTGTACAGACCCTTTCAGACATAAATCCTGATGTTGTGCTTGAGAGCTATACACTGAACATCACAACAGTGCAAGGTTTTGAAACCTTTACGTCTAGTTTAAAGGATAAATCGTTTCGCCCAAATAAAGAAGGCAGTGGAGTAGACCTTGTCTTAAGCTGTGTTGATAATTACGAAGCTCGAATGGCTGTTAATCAGGCGTGCAATGAACTGAATCAGACATGGATGGAGTCTGGTGTCTCTGAGGATGCTGTTTCAGGTCATATTCAGTTGCTTATACCTGGTGAAACTGCTTGTTTTGCATGTGCCCCACCTTTGGTTGTAGCATCTGGAATTGATGAACGCACACTTAAGCGTGAAGGGGTTTGTGCTGCATCTTTACCTACCACTATGGGAGTTGTTGCTGGGCTGCTAGTTCAGAATACCCTCAAATACTTGCTGAACTTTGGAAATGTCTCCCCATACTTGGGATATAATTCTCTTAAAGACTTCTTCCCCACCATGGAAATGAGGCCAAATCCTCAGTGTTCAAATTTTGCTTGTATGGAACGTCAGAAAGAATACTTGCTTGCGAAGCCGGCTAGGGATGCTGCAAGTAAAGTAAAGATGGAAGCCGAAGCATCATCATCTGTTATAGAAGCTCCACTCCATCTTGATAACGAATGGGAAATAAGCGTTGTTGATGATACTGAGCCACACAGCATAGAAGCCAAAAGTTTAGATACTCTTCCCGAAGGTCTAGTTCGGGAGCTTCCCACTGCGGATGACTTCCAAAAACCAGCTGCAGAAGCCGCAGATACTAATGTTGATGACCTAGAAGAACTTCGGAAACAACTCGATGCCCTTAATTCTTAA

Protein Analysis

427

Amino Acids

46.88

Weight (kDa)

4.76

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 319 5.8e-56 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 570
AciI CCGC 4 cut(s) 8, 425, 1187, 1218
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 246, 958
AcuI CTGAAG 3 cut(s) 35, 65, 197
AfaI GTAC 1 cut(s) 432
AfeI AGCGCT 1 cut(s) 169
AfiI CCNNNNNNNGG 4 cut(s) 742, 892, 893, 928
AflII CTTAAG 2 cut(s) 580, 776
AgsI TTSAA 2 cut(s) 509, 957
AjnI CCWGG 1 cut(s) 706
AleI CACNNNNGTG 1 cut(s) 494
Alw26I GTCTC 2 cut(s) 670, 886
AlwI GGATC 1 cut(s) 56
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 2 cut(s) 328, 938
ApeKI GCWGC 5 cut(s) 299, 797, 835, 1016, 1208
ApoI RAATTY 2 cut(s) 246, 958
Asp700I GAANNNNTTC 2 cut(s) 246, 1248
AspLEI GCGC 3 cut(s) 111, 147, 170
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 49, 722
AsuNHI GCTAGC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 736
BaeI ACNNNNGTAYC 2 cut(s) 1215, 1248
BbsI GAAGAC 1 cut(s) 326
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 5 cut(s) 311, 784, 822, 1003, 1195
BccI CCATC 4 cut(s) 214, 646, 1027, 1083
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 1 cut(s) 708
BcoDI GTCTC 2 cut(s) 670, 886
BfaI CTAG 6 cut(s) 365, 522, 839, 1008, 1166, 1241
BfmI CTRYAG 1 cut(s) 1209
BfoI RGCGCY 3 cut(s) 112, 148, 171
BfrI CTTAAG 2 cut(s) 580, 776
BisI GCNGC 7 cut(s) 300, 426, 798, 836, 1017, 1209, 1218
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 7 cut(s) 301, 427, 799, 837, 1018, 1210, 1219
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 9 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055, 1255
BmtI GCTAGC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 171
Bpu1102I GCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 927
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 4 cut(s) 742, 892, 893, 928
Bse118I RCCGGY 1 cut(s) 1003
Bse1I ACTGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 927
BseGI GGATG 5 cut(s) 225, 657, 679, 1018, 1195
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 4 cut(s) 742, 892, 893, 928
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 6 cut(s) 40, 162, 294, 660, 962, 1107
BseNI ACTGG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 5 cut(s) 311, 784, 822, 1003, 1195
BseYI CCCAGC 1 cut(s) 830
BshFI GGCC 2 cut(s) 330, 940
BshVI ATCGAT 1 cut(s) 84
BsiSI CCGG 2 cut(s) 132, 1004
BslI CCNNNNNNNGG 4 cut(s) 742, 892, 893, 928
BsmAI GTCTC 2 cut(s) 670, 886
BsnI GGCC 2 cut(s) 330, 940
Bsp1286I GDGCHC 1 cut(s) 736
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 2 cut(s) 50, 61
Bsp1720I GCTNAGC 1 cut(s) 26
Bsp19I CCATGG 1 cut(s) 927
BspACI CCGC 4 cut(s) 8, 425, 1187, 1218
BspANI GGCC 2 cut(s) 330, 940
BspCNI CTCAG 6 cut(s) 39, 163, 295, 661, 961, 1108
BspDI ATCGAT 1 cut(s) 84
BspMAI CTGCAG 1 cut(s) 1213
BspOI GCTAGC 1 cut(s) 368
BspPI GGATC 1 cut(s) 56
BspTI CTTAAG 2 cut(s) 580, 776
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 1003
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 1003
BssECI CCNNGG 1 cut(s) 927
BssMI GATC 2 cut(s) 50, 61
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 197, 496
Bst6I CTCTTC 2 cut(s) 41, 1158
BstAFI CTTAAG 2 cut(s) 580, 776
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 366, 628, 996, 1005
BstDEI CTNAG 6 cut(s) 26, 171, 303, 669, 948, 1116
BstDSI CCRYGG 1 cut(s) 927
BstF5I GGATG 5 cut(s) 225, 657, 679, 1018, 1195
BstH2I RGCGCY 3 cut(s) 112, 148, 171
BstHHI GCGC 3 cut(s) 111, 147, 170
BstKTI GATC 2 cut(s) 53, 64
BstMAI GTCTC 2 cut(s) 670, 886
BstMBI GATC 2 cut(s) 50, 61
BstMWI GCNNNNNNNGC 9 cut(s) 97, 106, 611, 725, 1000, 1004, 1013, 1214, 1217
BstNI CCWGG 1 cut(s) 708
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 706
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 1 cut(s) 736
BstV1I GCAGC 5 cut(s) 311, 784, 822, 1003, 1195
BstV2I GAAGAC 1 cut(s) 326
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 2 cut(s) 330, 940
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 927
BtsCI GGATG 5 cut(s) 225, 657, 679, 1018, 1195
BtsI GCAGTG 3 cut(s) 72, 568, 1182
BtsIMutI CAGTG 6 cut(s) 72, 479, 501, 568, 956, 1182
Cac8I GCNNGC 4 cut(s) 366, 628, 996, 1005
CfoI GCGC 3 cut(s) 111, 147, 170
Cfr10I RCCGGY 1 cut(s) 1003
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 1 cut(s) 431
CviAII CATG 4 cut(s) 373, 648, 729, 928
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 6 cut(s) 26, 171, 303, 669, 948, 1116
DpnI GATC 2 cut(s) 52, 63
DpnII GATC 2 cut(s) 50, 61
DraI TTTAAA 1 cut(s) 528
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 41, 1158
EarI CTCTTC 2 cut(s) 41, 1158
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 927
Eco47I GGWCC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 169
Eco57I CTGAAG 3 cut(s) 35, 65, 197
EcoRI GAATTC 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 927
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 4 cut(s) 376, 651, 732, 931
FatI CATG 4 cut(s) 372, 647, 728, 927
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 7 cut(s) 300, 426, 798, 836, 1017, 1209, 1218
FokI GGATG 5 cut(s) 232, 664, 686, 1025, 1202
Fsp4HI GCNGC 7 cut(s) 300, 426, 798, 836, 1017, 1209, 1218
FspBI CTAG 6 cut(s) 365, 522, 839, 1008, 1166, 1241
GlaI GCGC 3 cut(s) 110, 146, 169
GluI GCNGC 7 cut(s) 300, 426, 798, 836, 1017, 1209, 1218
GsaI CCCAGC 1 cut(s) 834
HaeII RGCGCY 3 cut(s) 112, 148, 171
HaeIII GGCC 2 cut(s) 330, 940
HapII CCGG 2 cut(s) 132, 1004
HhaI GCGC 3 cut(s) 111, 147, 170
Hin1II CATG 4 cut(s) 376, 651, 732, 931
Hin6I GCGC 3 cut(s) 109, 145, 168
HinP1I GCGC 3 cut(s) 109, 145, 168
HinfI GANTC 3 cut(s) 38, 640, 656
HpaII CCGG 2 cut(s) 132, 1004
HphI GGTGA 2 cut(s) 49, 722
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 7 cut(s) 216, 445, 645, 670, 846, 981, 1254
Hpy188III TCNNGA 8 cut(s) 158, 251, 395, 455, 756, 1079, 1157, 1172
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 5 cut(s) 10, 75, 551, 779, 1154
HpyCH4III ACNGT 2 cut(s) 197, 496
HpyCH4IV ACGT 2 cut(s) 518, 976
HpyCH4V TGCA 7 cut(s) 125, 499, 630, 728, 800, 1019, 1211
HpyF10VI GCNNNNNNNGC 9 cut(s) 97, 106, 611, 725, 1000, 1004, 1013, 1214, 1217
HpyF3I CTNAG 6 cut(s) 26, 171, 303, 669, 948, 1116
HpySE526I ACGT 2 cut(s) 518, 976
Hsp92II CATG 4 cut(s) 376, 651, 732, 931
HspAI GCGC 3 cut(s) 109, 145, 168
KroI GCCGGC 1 cut(s) 1003
KroNI GCCGGC 1 cut(s) 1005
Kzo9I GATC 2 cut(s) 50, 61
LmnI GCTCC 5 cut(s) 10, 82, 152, 1072, 1174
Lsp1109I GCAGC 5 cut(s) 311, 784, 822, 1003, 1195
LweI GCATC 9 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055, 1255
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 6 cut(s) 365, 522, 839, 1008, 1166, 1241
MaeII ACGT 2 cut(s) 518, 976
MalI GATC 2 cut(s) 52, 63
MboI GATC 2 cut(s) 50, 61
MboII GAAGA 5 cut(s) 58, 326, 910, 1145, 1256
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 6 cut(s) 246, 595, 759, 901, 958, 1273
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 7 cut(s) 44, 166, 210, 664, 863, 930, 957
MroNI GCCGGC 1 cut(s) 1003
MroXI GAANNNNTTC 2 cut(s) 246, 1248
MseI TTAA 7 cut(s) 527, 581, 618, 777, 909, 1272, 1279
MslI CAYNNNNRTG 2 cut(s) 494, 932
MspA1I CMGCKG 2 cut(s) 302, 1208
MspCI CTTAAG 2 cut(s) 580, 776
MspI CCGG 2 cut(s) 132, 1004
MspR9I CCNGG 1 cut(s) 708
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 9 cut(s) 97, 106, 611, 725, 1000, 1004, 1013, 1214, 1217
NaeI GCCGGC 1 cut(s) 1005
NcoI CCATGG 1 cut(s) 927
NdeII GATC 2 cut(s) 50, 61
NgoMIV GCCGGC 1 cut(s) 1003
NheI GCTAGC 1 cut(s) 364
NlaIII CATG 4 cut(s) 376, 651, 732, 931
NspI RCATGY 1 cut(s) 732
OliI CACNNNNGTG 1 cut(s) 494
PdiI GCCGGC 1 cut(s) 1005
PdmI GAANNNNTTC 2 cut(s) 246, 1248
PfeI GAWTC 2 cut(s) 38, 640
PflFI GACNNNGTC 1 cut(s) 575
PkrI GCNGC 7 cut(s) 301, 427, 799, 837, 1018, 1210, 1219
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 1 cut(s) 134
PstI CTGCAG 1 cut(s) 1213
PsyI GACNNNGTC 1 cut(s) 575
PvuII CAGCTG 2 cut(s) 302, 1208
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
RseI CAYNNNNRTG 2 cut(s) 494, 932
SaqAI TTAA 7 cut(s) 527, 581, 618, 777, 909, 1272, 1279
SatI GCNGC 7 cut(s) 300, 426, 798, 836, 1017, 1209, 1218
Sau3AI GATC 2 cut(s) 50, 61
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 1 cut(s) 736
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 9 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055, 1255
SfcI CTRYAG 1 cut(s) 1209
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 494, 932
SmlI CTYRAG 3 cut(s) 467, 580, 776
SmoI CTYRAG 3 cut(s) 467, 580, 776
Sse9I AATT 6 cut(s) 246, 595, 759, 901, 958, 1273
SsiI CCGC 4 cut(s) 8, 425, 1187, 1218
SspMI CTAG 6 cut(s) 365, 522, 839, 1008, 1166, 1241
StyD4I CCNGG 1 cut(s) 706
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 2 cut(s) 197, 496
TaiI ACGT 2 cut(s) 521, 979
TaqI TCGA 4 cut(s) 36, 84, 607, 1263
TasI AATT 6 cut(s) 246, 595, 759, 901, 958, 1273
TatI WGTACW 1 cut(s) 430
TauI GCSGC 2 cut(s) 428, 1220
TfiI GAWTC 2 cut(s) 38, 640
Tru1I TTAA 7 cut(s) 527, 581, 618, 777, 909, 1272, 1279
Tru9I TTAA 7 cut(s) 527, 581, 618, 777, 909, 1272, 1279
TscAI CASTG 6 cut(s) 79, 486, 501, 568, 956, 1189
TseI GCWGC 5 cut(s) 299, 797, 835, 1016, 1208
TspDTI ATGAA 4 cut(s) 255, 389, 648, 780
TspRI CASTG 6 cut(s) 79, 486, 501, 568, 956, 1189
Tth111I GACNNNGTC 1 cut(s) 575
Vha464I CTTAAG 2 cut(s) 580, 776
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 2 cut(s) 246, 958
XceI RCATGY 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 2 cut(s) 246, 1248
XspI CTAG 6 cut(s) 365, 522, 839, 1008, 1166, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.