MD06G1141900.v1.1

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010013
Physical Location & Seq
Forward (+)
28539336 .. 28543768
4433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1141900.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGGAGGTGGAACTGAAGGAGATGCTGAACGACCTGGAATCGCTGAAGAGATCGCTGGCCGATCCTTCTCATCATGCTCCAACCGATAAGCTACAATTCCGCGTAGAACGCCTTACGAGCCTTGCAAAGTCTGGACCTGTCCGGCGTTCAAAAGTGAAGGATATGAGCGCTGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGTATTGTGGAGAACTATGAAAGAATACGGGATTTCTCAGTTGCCATTGTTGGAGTAGGCGGTGTAGGAAGTGTAGCAGCTGAGATGCTTACAAGATGTGGTATTGGTCGCCTTTTGTTGTATGATTATGACAAAGTAGAGTTAGCTAACATGAATAGGTTATTCTTTCGACCAGAACAGAGTGGCATGACAAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCCGATGTTATACTCGAGAGCTATACACTGAACATCACAACAGTGCAAGGTTTTGATACCTTTATGTCTAGTTTAAAAAATAAATCGTTTCGCCCAAATAAAGAAGGCACTGGAATAGACCTTGTGTTAAGCTGTGTTGATAATTATGAAGCAAGAATGGCTGTTAATCAGGCTTGCAATGAACTGAATCAGACATGGATGGAGTCTGGTGTGTCTGAGGATGCCGTTTCAGGCCATATTCAGTTGCTGATTCCCGGTGAAACTGCATGTTTTGCATGTGCACCACCTTTGGTTGTAGCATCTGGAGTGGATGAACGTACACTTAAGCGTGAAGGGGTTTGTGCTGCATCTTTACCTACCACTATGGGAGTTGTTGCTGGGCTTCTAGTCCAAAATACACTAAAATACTTGCTGAACTTCGGAAACGTCTCCCCATACTTGGGATATAATTCTCTTAAAGACTTCTTCCCAACTATGGAAATGAAGCCAAACCCTCAGTGTTCTAATTTTGCTTGTCTGGAACGGCAGAAAGAATACTTGCTTGTAAAGCCAGCTAGGGATGCTGCAATTAAAGCAAAGATGGAAGCTGAAGCATCATCAGTTCCGGAATCTCCGCTTCATCTTGATAATGAATGGGAAATAAGTGTCGTTGATGATACCGAGCCGGAAAGTGCAGAGGCCAAAACTTCAGATGCACTTCCAGAAGGTCTTGTTCGCGAGCTCCCCAGTGCAGATGAGTTCCAAAAACCGCCAACTGAAGCTACAGAGGGTACTATCGACGACCTAGAAGATCTTCGAAAGCAACTTGAGGCCCTAAATTCTTGA

Protein Analysis

426

Amino Acids

46.82

Weight (kDa)

4.74

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 319 2.4e-55 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 102, 1170
AccIII TCCGGA 1 cut(s) 1057
AciI CCGC 4 cut(s) 100, 282, 1067, 1202
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 1270
AcuI CTGAAG 6 cut(s) 35, 65, 197, 1062, 1125, 1230
AfaI GTAC 3 cut(s) 432, 772, 1225
AfeI AGCGCT 1 cut(s) 169
AfiI CCNNNNNNNGG 3 cut(s) 892, 893, 928
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 1 cut(s) 150
AjnI CCWGG 1 cut(s) 33
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 9 cut(s) 91, 302, 368, 474, 585, 1007, 1040, 1174, 1214
AluI AGCT 9 cut(s) 91, 302, 368, 474, 585, 1007, 1040, 1174, 1214
Alw21I GWGCWC 2 cut(s) 736, 1176
Alw26I GTCTC 1 cut(s) 886
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 2 cut(s) 425, 700
Ama87I CYCGRG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 1057
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 4 cut(s) 57, 685, 1131, 1263
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 4 cut(s) 299, 425, 797, 1016
ApoI RAATTY 1 cut(s) 1270
Asp700I GAANNNNTTC 1 cut(s) 1245
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 2 cut(s) 134, 1264
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 1 cut(s) 722
AsuII TTCGAA 1 cut(s) 1249
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 736
BanII GRGCYC 1 cut(s) 1176
Bbv12I GWGCWC 2 cut(s) 736, 1176
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 4 cut(s) 311, 412, 784, 1003
BccI CCATC 3 cut(s) 214, 646, 1027
BceAI ACGGC 2 cut(s) 662, 992
BciT130I CCWGG 1 cut(s) 35
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 886
BfaI CTAG 4 cut(s) 522, 839, 1008, 1238
BfmI CTRYAG 1 cut(s) 1215
BfoI RGCGCY 1 cut(s) 171
BfrI CTTAAG 1 cut(s) 776
BglII AGATCT 1 cut(s) 1243
BisI GCNGC 4 cut(s) 300, 426, 798, 1017
BlsI GCNGC 4 cut(s) 301, 427, 799, 1018
Bme1390I CCNGG 2 cut(s) 35, 708
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 134, 1264
BmrFI CCNGG 2 cut(s) 35, 708
BmrI ACTGGG 1 cut(s) 1173
BmsI GCATC 9 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055, 1135
BmuI ACTGGG 1 cut(s) 1173
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 1249
BpuMI CCSGG 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 892, 893, 928
Bse1I ACTGG 2 cut(s) 568, 1179
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 1057
BseBI CCWGG 1 cut(s) 35
BseGI GGATG 5 cut(s) 225, 657, 679, 769, 1018
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 3 cut(s) 892, 893, 928
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 5 cut(s) 162, 273, 294, 660, 962
BseNI ACTGG 2 cut(s) 568, 1179
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 4 cut(s) 311, 412, 784, 1003
BseYI CCCAGC 1 cut(s) 830
BsgI GTGCAG 2 cut(s) 1146, 1203
Bsh1236I CGCG 2 cut(s) 102, 1170
BshFI GGCC 4 cut(s) 59, 687, 1133, 1265
BsiHKAI GWGCWC 2 cut(s) 736, 1176
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 4 cut(s) 142, 708, 1058, 1118
BslI CCNNNNNNNGG 3 cut(s) 892, 893, 928
BsmAI GTCTC 1 cut(s) 886
BsmBI CGTCTC 1 cut(s) 886
BsnI GGCC 4 cut(s) 59, 687, 1133, 1265
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 1 cut(s) 1249
Bsp1286I GDGCHC 2 cut(s) 736, 1176
Bsp13I TCCGGA 1 cut(s) 1057
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 50, 61, 1243
Bsp68I TCGCGA 1 cut(s) 1170
BspACI CCGC 4 cut(s) 100, 282, 1067, 1202
BspANI GGCC 4 cut(s) 59, 687, 1133, 1265
BspCNI CTCAG 5 cut(s) 163, 272, 295, 661, 961
BspEI TCCGGA 1 cut(s) 1057
BspFNI CGCG 2 cut(s) 102, 1170
BspPI GGATC 1 cut(s) 56
BspT104I TTCGAA 1 cut(s) 1249
BspTI CTTAAG 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 2 cut(s) 568, 1179
BssMI GATC 3 cut(s) 50, 61, 1243
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 197, 496
Bst6I CTCTTC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 776
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 1249
BstC8I GCNNGC 4 cut(s) 57, 628, 1005, 1172
BstDEI CTNAG 5 cut(s) 171, 259, 303, 669, 948
BstF5I GGATG 5 cut(s) 225, 657, 679, 769, 1018
BstFNI CGCG 2 cut(s) 102, 1170
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 3 cut(s) 53, 64, 1246
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 3 cut(s) 50, 61, 1243
BstMWI GCNNNNNNNGC 7 cut(s) 108, 117, 611, 725, 1000, 1013, 1025
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 2 cut(s) 723, 732
BstSCI CCNGG 2 cut(s) 33, 706
BstSFI CTRYAG 1 cut(s) 1215
BstSLI GKGCMC 1 cut(s) 736
BstUI CGCG 2 cut(s) 102, 1170
BstV1I GCAGC 4 cut(s) 311, 412, 784, 1003
BstX2I RGATCY 1 cut(s) 1243
BstYI RGATCY 1 cut(s) 1243
BsuRI GGCC 4 cut(s) 59, 687, 1133, 1265
BtsCI GGATG 5 cut(s) 225, 657, 679, 769, 1018
BtsIMutI CAGTG 5 cut(s) 479, 501, 561, 956, 1186
BtuMI TCGCGA 1 cut(s) 1170
Cac8I GCNNGC 4 cut(s) 57, 628, 1005, 1172
CaiI CAGNNNCTG 2 cut(s) 425, 700
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 134, 1264
Csp6I GTAC 3 cut(s) 431, 771, 1224
CviAII CATG 6 cut(s) 74, 373, 409, 648, 720, 729
CviQI GTAC 3 cut(s) 431, 771, 1224
DdeI CTNAG 5 cut(s) 171, 259, 303, 669, 948
DpnI GATC 3 cut(s) 52, 63, 1245
DpnII GATC 3 cut(s) 50, 61, 1243
DraI TTTAAA 1 cut(s) 528
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 1174
Eco24I GRGCYC 1 cut(s) 1176
Eco47I GGWCC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 1174
Eco57I CTGAAG 6 cut(s) 35, 65, 197, 1062, 1125, 1230
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 1174
EcoO109I RGGNCCY 1 cut(s) 1264
EcoRII CCWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 1176
Esp3I CGTCTC 1 cut(s) 886
FaeI CATG 6 cut(s) 77, 376, 412, 651, 723, 732
FatI CATG 6 cut(s) 73, 372, 408, 647, 719, 728
Fnu4HI GCNGC 4 cut(s) 300, 426, 798, 1017
FokI GGATG 5 cut(s) 232, 664, 686, 776, 1025
FriOI GRGCYC 1 cut(s) 1176
Fsp4HI GCNGC 4 cut(s) 300, 426, 798, 1017
FspBI CTAG 4 cut(s) 522, 839, 1008, 1238
GlaI GCGC 1 cut(s) 169
GluI GCNGC 4 cut(s) 300, 426, 798, 1017
GsaI CCCAGC 1 cut(s) 834
GsuI CTGGAG 1 cut(s) 777
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 4 cut(s) 59, 687, 1133, 1265
HapII CCGG 4 cut(s) 142, 708, 1058, 1118
HhaI GCGC 1 cut(s) 170
Hin1II CATG 6 cut(s) 77, 376, 412, 651, 723, 732
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 5 cut(s) 38, 640, 656, 703, 1061
HpaII CCGG 4 cut(s) 142, 708, 1058, 1118
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 2 cut(s) 734, 773
Hpy188I TCNGA 6 cut(s) 216, 445, 645, 670, 875, 1144
Hpy8I GTNNAC 2 cut(s) 734, 773
Hpy99I CGWCG 1 cut(s) 1235
HpyAV CCTTC 6 cut(s) 10, 75, 151, 551, 779, 1151
HpyCH4III ACNGT 2 cut(s) 197, 496
HpyCH4IV ACGT 2 cut(s) 769, 879
HpyF10VI GCNNNNNNNGC 7 cut(s) 108, 117, 611, 725, 1000, 1013, 1025
HpyF3I CTNAG 5 cut(s) 171, 259, 303, 669, 948
HpySE526I ACGT 2 cut(s) 769, 879
Hsp92II CATG 6 cut(s) 77, 376, 412, 651, 723, 732
HspAI GCGC 1 cut(s) 168
Kpn2I TCCGGA 1 cut(s) 1057
Kzo9I GATC 3 cut(s) 50, 61, 1243
LmnI GCTCC 2 cut(s) 82, 1179
Lsp1109I GCAGC 4 cut(s) 311, 412, 784, 1003
LweI GCATC 9 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055, 1135
MaeI CTAG 4 cut(s) 522, 839, 1008, 1238
MaeII ACGT 2 cut(s) 769, 879
MalI GATC 3 cut(s) 52, 63, 1245
MboI GATC 3 cut(s) 50, 61, 1243
MboII GAAGA 4 cut(s) 58, 910, 1238, 1253
MflI RGATCY 1 cut(s) 1243
MhlI GDGCHC 2 cut(s) 736, 1176
MluCI AATT 6 cut(s) 95, 595, 901, 958, 1020, 1270
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 2 cut(s) 104, 253
MnlI CCTC 7 cut(s) 166, 210, 664, 957, 1123, 1213, 1255
MroI TCCGGA 1 cut(s) 1057
MroXI GAANNNNTTC 1 cut(s) 1245
MseI TTAA 6 cut(s) 527, 581, 618, 777, 909, 1023
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 4 cut(s) 142, 708, 1058, 1118
MspR9I CCNGG 2 cut(s) 35, 708
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 2 cut(s) 102, 1170
MwoI GCNNNNNNNGC 7 cut(s) 108, 117, 611, 725, 1000, 1013, 1025
NciI CCSGG 1 cut(s) 708
NdeII GATC 3 cut(s) 50, 61, 1243
NlaIII CATG 6 cut(s) 77, 376, 412, 651, 723, 732
NruI TCGCGA 1 cut(s) 1170
NspI RCATGY 2 cut(s) 723, 732
NspV TTCGAA 1 cut(s) 1249
OliI CACNNNNGTG 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 4 cut(s) 38, 640, 703, 1061
PkrI GCNGC 4 cut(s) 301, 427, 799, 1018
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp124BI GAGCTC 1 cut(s) 1176
Psp6I CCWGG 1 cut(s) 33
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 1 cut(s) 33
PspPI GGNCC 2 cut(s) 134, 1264
PstNI CAGNNNCTG 2 cut(s) 425, 700
PsuI RGATCY 1 cut(s) 1243
PvuII CAGCTG 1 cut(s) 302
RruI TCGCGA 1 cut(s) 1170
RsaI GTAC 3 cut(s) 432, 772, 1225
RsaNI GTAC 3 cut(s) 431, 771, 1224
RseI CAYNNNNRTG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 1176
SaqAI TTAA 6 cut(s) 527, 581, 618, 777, 909, 1023
SatI GCNGC 4 cut(s) 300, 426, 798, 1017
Sau3AI GATC 3 cut(s) 50, 61, 1243
Sau96I GGNCC 2 cut(s) 134, 1264
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 2 cut(s) 35, 708
SduI GDGCHC 2 cut(s) 736, 1176
SfaNI GCATC 9 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055, 1135
SfcI CTRYAG 1 cut(s) 1215
Sfr274I CTCGAG 1 cut(s) 467
SfuI TTCGAA 1 cut(s) 1249
SinI GGWCC 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 3 cut(s) 467, 776, 1259
SmoI CTYRAG 3 cut(s) 467, 776, 1259
Sse9I AATT 6 cut(s) 95, 595, 901, 958, 1020, 1270
SsiI CCGC 4 cut(s) 100, 282, 1067, 1202
SspMI CTAG 4 cut(s) 522, 839, 1008, 1238
SstI GAGCTC 1 cut(s) 1176
StyD4I CCNGG 2 cut(s) 33, 706
TaaI ACNGT 2 cut(s) 197, 496
TaiI ACGT 2 cut(s) 772, 882
TaqI TCGA 4 cut(s) 391, 468, 1230, 1249
TasI AATT 6 cut(s) 95, 595, 901, 958, 1020, 1270
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 4 cut(s) 38, 640, 703, 1061
Tru1I TTAA 6 cut(s) 527, 581, 618, 777, 909, 1023
Tru9I TTAA 6 cut(s) 527, 581, 618, 777, 909, 1023
TscAI CASTG 5 cut(s) 486, 501, 568, 956, 1186
TseI GCWGC 4 cut(s) 299, 425, 797, 1016
TspDTI ATGAA 8 cut(s) 255, 389, 615, 648, 780, 950, 1061, 1098
TspRI CASTG 5 cut(s) 486, 501, 568, 956, 1186
Vha464I CTTAAG 1 cut(s) 776
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 1270
XceI RCATGY 2 cut(s) 723, 732
XhoI CTCGAG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 4 cut(s) 522, 839, 1008, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.