RLG00000004554

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010013
Physical Location & Seq
Reverse (-)
60228722 .. 60233402
4681 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004554

Sequence Viewer

Length: 1113 bp
ATGGAGGTGGAATTGAAGGAGATGCTTAACGACCTCGATTCGCTGAAGAGATCGCTCACCGATCCTTCGCAGAGCGCTCCAATCGATAAACTACAACTGCGTGTTGAACGCCTTACCAGTCTTGCAAAAACTGGACCTGTTCGGCGATCCAAAGTTCAGGATATGAGCGCTGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGCATTGTGAAAAACTATGAAAGAATAAGGGAGTTCTCCGTAGCCATAGTTGGAGTAGGTGGCGTAGGAAGTGTAGCAGCTGAGATGCTAACAAGATGTGGTATTGGTCGCCTTCTGTTGTACGATTATGATAAAGTAGAGCTAGCTAACATGAATAGGTTATTCTTTCGTCCAGAGCAGAGTGGTATGACAAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCTGATGTTGTGCTTGAGAGCTATACACTGAACATCACAACAGTGCAAGGTTTTGAAACCTTTACGTCCAGTTTGAAAAATAAATCATTTCGCCCAGATAAGGAAGGCAGTGGAGTAGACCTTGTGTTAAGCTGTGTTGATAATTACGAAGCTCGAATGGCTGTTAATCAGGCTTGCAATGAACTGAATCAGACATGGATGGAGTCTGGCGTTTCTGAGGATGCTGTTTCAGGTCATATTCAGTTGCTTATACCTGGTGAAACTGCCTGTTTTGCATGTGCCCCACCTTTGGTTGTAGCATCTGGAATAGATGAACGCACACTTAAGCGAGAAGGGGTTTGTGCTGCATCTTTACCTACTACTATGGGAGTTGTTGCTGGGCTGCTAGTTCAGAATACACTAAAATACTTGCTGAACTTTGGAAATGTCTCCCCATACTTGGGATATAATTCTCTTAAAGACTTCTTCCCCACCATGGAAATGAGGCCAAATCCTCAGTGTTCAAATTTTGCTTGTCTGGAACGTCAGAAAGAATACTTGCTTGCAAAGCCGGCTAGGGATGCTGCTAGTAAAGCAAAGATGGAATCTGAAGCATCATCATCTGTAATAGAAGCTCCACTCCATCTTGATAACGAATGGGAAATAAGGTGCTTTGACTAA

Protein Analysis

371

Amino Acids

40.85

Weight (kDa)

5.08

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 319 3.2e-56 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 570
AclWI GGATC 2 cut(s) 56, 141
AcsI RAATTY 1 cut(s) 958
AcuI CTGAAG 3 cut(s) 65, 197, 1062
AfaI GTAC 2 cut(s) 344, 432
AfeI AGCGCT 2 cut(s) 76, 169
AfiI CCNNNNNNNGG 5 cut(s) 553, 742, 892, 893, 928
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 5 cut(s) 16, 107, 509, 529, 957
AjnI CCWGG 1 cut(s) 706
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 7 cut(s) 302, 364, 368, 474, 585, 605, 1067
AluI AGCT 7 cut(s) 302, 364, 368, 474, 585, 605, 1067
Alw26I GTCTC 1 cut(s) 886
AlwI GGATC 2 cut(s) 56, 141
AlwNI CAGNNNCTG 1 cut(s) 425
Aor51HI AGCGCT 2 cut(s) 76, 169
AoxI GGCC 1 cut(s) 938
ApeKI GCWGC 5 cut(s) 299, 425, 797, 835, 1016
ApoI RAATTY 1 cut(s) 958
AspLEI GCGC 2 cut(s) 77, 170
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 49, 722
AsuNHI GCTAGC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 736
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 5 cut(s) 311, 412, 784, 822, 1003
BccI CCATC 4 cut(s) 214, 646, 1027, 1083
BciT130I CCWGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 886
BfaI CTAG 4 cut(s) 365, 839, 1008, 1020
BfoI RGCGCY 2 cut(s) 78, 171
BfrI CTTAAG 1 cut(s) 776
BisI GCNGC 5 cut(s) 300, 426, 798, 836, 1017
BlsI GCNGC 5 cut(s) 301, 427, 799, 837, 1018
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 8 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055
BmtI GCTAGC 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 927
Bsc4I CCNNNNNNNGG 5 cut(s) 553, 742, 892, 893, 928
Bse118I RCCGGY 1 cut(s) 1003
Bse1I ACTGG 3 cut(s) 117, 136, 522
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 927
BseGI GGATG 4 cut(s) 225, 657, 679, 1018
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 5 cut(s) 553, 742, 892, 893, 928
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 4 cut(s) 162, 294, 660, 962
BseNI ACTGG 3 cut(s) 117, 136, 522
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 5 cut(s) 311, 412, 784, 822, 1003
BseYI CCCAGC 1 cut(s) 830
BshFI GGCC 1 cut(s) 940
BshVI ATCGAT 1 cut(s) 84
BsiSI CCGG 1 cut(s) 1004
BslI CCNNNNNNNGG 5 cut(s) 553, 742, 892, 893, 928
BsmAI GTCTC 1 cut(s) 886
BsnI GGCC 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 736
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 50, 61, 146
Bsp19I CCATGG 1 cut(s) 927
BspANI GGCC 1 cut(s) 940
BspCNI CTCAG 4 cut(s) 163, 295, 661, 961
BspDI ATCGAT 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 368
BspPI GGATC 2 cut(s) 56, 141
BspTI CTTAAG 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 1003
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 3 cut(s) 117, 136, 522
BssAI RCCGGY 1 cut(s) 1003
BssECI CCNNGG 1 cut(s) 927
BssMI GATC 3 cut(s) 50, 61, 146
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 197, 496
Bst6I CTCTTC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 366, 628, 996, 1005
BstDEI CTNAG 4 cut(s) 171, 303, 669, 948
BstDSI CCRYGG 1 cut(s) 927
BstF5I GGATG 4 cut(s) 225, 657, 679, 1018
BstH2I RGCGCY 2 cut(s) 78, 171
BstHHI GCGC 2 cut(s) 77, 170
BstKTI GATC 3 cut(s) 53, 64, 149
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 3 cut(s) 50, 61, 146
BstMWI GCNNNNNNNGC 6 cut(s) 611, 725, 1000, 1004, 1013, 1025
BstNI CCWGG 1 cut(s) 708
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 736
BstV1I GCAGC 5 cut(s) 311, 412, 784, 822, 1003
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 1 cut(s) 940
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 927
BtsCI GGATG 4 cut(s) 225, 657, 679, 1018
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 4 cut(s) 479, 501, 568, 956
Cac8I GCNNGC 4 cut(s) 366, 628, 996, 1005
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 2 cut(s) 77, 170
Cfr10I RCCGGY 1 cut(s) 1003
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 2 cut(s) 343, 431
CviAII CATG 4 cut(s) 373, 648, 729, 928
CviQI GTAC 2 cut(s) 343, 431
DdeI CTNAG 4 cut(s) 171, 303, 669, 948
DpnI GATC 3 cut(s) 52, 63, 148
DpnII GATC 3 cut(s) 50, 61, 146
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 927
Eco47I GGWCC 1 cut(s) 134
Eco47III AGCGCT 2 cut(s) 76, 169
Eco57I CTGAAG 3 cut(s) 65, 197, 1062
EcoRII CCWGG 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 927
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 4 cut(s) 376, 651, 732, 931
FatI CATG 4 cut(s) 372, 647, 728, 927
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 300, 426, 798, 836, 1017
FokI GGATG 4 cut(s) 232, 664, 686, 1025
Fsp4HI GCNGC 5 cut(s) 300, 426, 798, 836, 1017
FspBI CTAG 4 cut(s) 365, 839, 1008, 1020
GlaI GCGC 2 cut(s) 76, 169
GluI GCNGC 5 cut(s) 300, 426, 798, 836, 1017
GsaI CCCAGC 1 cut(s) 834
HaeII RGCGCY 2 cut(s) 78, 171
HaeIII GGCC 1 cut(s) 940
HapII CCGG 1 cut(s) 1004
HhaI GCGC 2 cut(s) 77, 170
Hin1II CATG 4 cut(s) 376, 651, 732, 931
Hin6I GCGC 2 cut(s) 75, 168
HinP1I GCGC 2 cut(s) 75, 168
HinfI GANTC 4 cut(s) 38, 640, 656, 1037
HpaII CCGG 1 cut(s) 1004
HphI GGTGA 2 cut(s) 49, 722
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 7 cut(s) 216, 445, 645, 670, 846, 981, 1042
Hpy188III TCNNGA 6 cut(s) 158, 395, 455, 756, 971, 1079
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 5 cut(s) 10, 75, 344, 551, 779
HpyCH4III ACNGT 2 cut(s) 197, 496
HpyCH4IV ACGT 2 cut(s) 518, 976
HpyCH4V TGCA 6 cut(s) 125, 499, 630, 728, 800, 998
HpyF10VI GCNNNNNNNGC 6 cut(s) 611, 725, 1000, 1004, 1013, 1025
HpyF3I CTNAG 4 cut(s) 171, 303, 669, 948
HpySE526I ACGT 2 cut(s) 518, 976
Hsp92II CATG 4 cut(s) 376, 651, 732, 931
HspAI GCGC 2 cut(s) 75, 168
KroI GCCGGC 1 cut(s) 1003
KroNI GCCGGC 1 cut(s) 1005
Kzo9I GATC 3 cut(s) 50, 61, 146
LmnI GCTCC 2 cut(s) 82, 1072
Lsp1109I GCAGC 5 cut(s) 311, 412, 784, 822, 1003
LweI GCATC 8 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 4 cut(s) 365, 839, 1008, 1020
MaeII ACGT 2 cut(s) 518, 976
MalI GATC 3 cut(s) 52, 63, 148
MboI GATC 3 cut(s) 50, 61, 146
MboII GAAGA 2 cut(s) 58, 910
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 4 cut(s) 11, 595, 901, 958
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 6 cut(s) 44, 166, 210, 664, 930, 957
MroNI GCCGGC 1 cut(s) 1003
MseI TTAA 5 cut(s) 27, 581, 618, 777, 909
MslI CAYNNNNRTG 2 cut(s) 494, 932
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 1 cut(s) 1004
MspR9I CCNGG 1 cut(s) 708
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 6 cut(s) 611, 725, 1000, 1004, 1013, 1025
NaeI GCCGGC 1 cut(s) 1005
NcoI CCATGG 1 cut(s) 927
NdeII GATC 3 cut(s) 50, 61, 146
NgoMIV GCCGGC 1 cut(s) 1003
NheI GCTAGC 1 cut(s) 364
NlaIII CATG 4 cut(s) 376, 651, 732, 931
NspI RCATGY 1 cut(s) 732
OliI CACNNNNGTG 1 cut(s) 494
PdiI GCCGGC 1 cut(s) 1005
PfeI GAWTC 3 cut(s) 38, 640, 1037
PkrI GCNGC 5 cut(s) 301, 427, 799, 837, 1018
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 830
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 344, 432
RsaNI GTAC 2 cut(s) 343, 431
RseI CAYNNNNRTG 2 cut(s) 494, 932
SaqAI TTAA 5 cut(s) 27, 581, 618, 777, 909
SatI GCNGC 5 cut(s) 300, 426, 798, 836, 1017
Sau3AI GATC 3 cut(s) 50, 61, 146
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 1 cut(s) 736
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 8 cut(s) 12, 297, 412, 664, 761, 809, 1003, 1055
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 494, 932
SmlI CTYRAG 2 cut(s) 467, 776
SmoI CTYRAG 2 cut(s) 467, 776
Sse9I AATT 4 cut(s) 11, 595, 901, 958
SspMI CTAG 4 cut(s) 365, 839, 1008, 1020
StyD4I CCNGG 1 cut(s) 706
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 2 cut(s) 197, 496
TaiI ACGT 2 cut(s) 521, 979
TaqI TCGA 3 cut(s) 36, 84, 607
TasI AATT 4 cut(s) 11, 595, 901, 958
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 3 cut(s) 38, 640, 1037
Tru1I TTAA 5 cut(s) 27, 581, 618, 777, 909
Tru9I TTAA 5 cut(s) 27, 581, 618, 777, 909
TscAI CASTG 4 cut(s) 486, 501, 568, 956
TseI GCWGC 5 cut(s) 299, 425, 797, 835, 1016
TspDTI ATGAA 4 cut(s) 255, 389, 648, 780
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 4 cut(s) 486, 501, 568, 956
Vha464I CTTAAG 1 cut(s) 776
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 958
XceI RCATGY 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 4 cut(s) 365, 839, 1008, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.