Rh7AG108200

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010013
Physical Location & Seq
Forward (+)
8550994 .. 8555495
4502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG108200.1

Sequence Viewer

Length: 909 bp
ATGGAGGTGGAATTGAAGGAGATGCTTAACGACCTCGATTCGCTGAAGAGATCGCTCACCGATCCTTCGCAGAGCGCTCCAATCGATAAACTACAACTGCGTGTTGAACGCCTTACCAGTCTTGCAAAAACTGGACCTGTTCGGCGATCCAAAGTTCAGGATATGAGCGCTGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGCATTGTGAAAAACTATGAAAGAATAAGGGAGTTCTCCGTAGCCATAGTTGGAGTAGGTGGCGTAGGAAGTGTAGCAGCTGAGATGCTAACAAGATGTGGTATTGGTCGCCTTCTATTGTACGATTATGATAAAGTAGAGCTAGCTAACATGAATAGGTTATTCTTTCGTCCAGAGCAGAGTGGTATGACAAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCTGATGTTGTGCTTGAGAGCTATACACTGAACATCACAACAGTGCAAGGATTTGAAACCTTTACGTCCAGTTTGAAAAATAAATCATTTCGCCCAGATAAGGAAGGCAGTGGAGTAGACCTTGTGTTAAGCTGTGTTGATAATTACGAAGCTCGAATGGCTGTTAATCAGGCTTGCAATGAACTGAATCAGACATGGATGGAGTCTGGTGTTTCTGAGGATGCTGTTTCAGGTCATATTCAGTTGCTTATACCTGGTGAAACTGCCTGTTTTGCATGTGCCCCACCTTTGGTTGTAGCATCTGGAATAGATGAACGCACACTTAAGCGAGAAGGGGTTTGTGCTGCATCTTTACCTACTACTATGGTAAATAAATTTCTCGTCCATCTCTTGGTGGCTGTATATTTGGGTCTTTTATTATTCGAACTTGCTACCATGAATGAAAAGGAAAAATGA

Protein Analysis

302

Amino Acids

33.27

Weight (kDa)

5.13

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 271 2.7e-46 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 844
AccI GTMKAC 1 cut(s) 570
AclWI GGATC 2 cut(s) 56, 141
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 2 cut(s) 65, 197
AfaI GTAC 2 cut(s) 344, 432
AfeI AGCGCT 2 cut(s) 76, 169
AfiI CCNNNNNNNGG 3 cut(s) 553, 742, 844
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 4 cut(s) 16, 107, 509, 529
AjnI CCWGG 1 cut(s) 706
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 6 cut(s) 302, 364, 368, 474, 585, 605
AluI AGCT 6 cut(s) 302, 364, 368, 474, 585, 605
AlwI GGATC 2 cut(s) 56, 141
AlwNI CAGNNNCTG 1 cut(s) 425
Aor51HI AGCGCT 2 cut(s) 76, 169
ApeKI GCWGC 3 cut(s) 299, 425, 797
ApoI RAATTY 1 cut(s) 827
AspLEI GCGC 2 cut(s) 77, 170
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 49, 722
AsuII TTCGAA 1 cut(s) 876
AsuNHI GCTAGC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 736
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 311, 412, 784
BccI CCATC 3 cut(s) 214, 646, 846
BciT130I CCWGG 1 cut(s) 708
BfaI CTAG 1 cut(s) 365
BfoI RGCGCY 2 cut(s) 78, 171
BfrI CTTAAG 1 cut(s) 776
BisI GCNGC 3 cut(s) 300, 426, 798
BlsI GCNGC 3 cut(s) 301, 427, 799
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 6 cut(s) 12, 297, 412, 664, 761, 809
BmtI GCTAGC 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 186
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 742, 844
Bse1I ACTGG 3 cut(s) 117, 136, 522
Bse3DI GCAATG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 84
BseGI GGATG 3 cut(s) 225, 657, 679
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 3 cut(s) 553, 742, 844
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 3 cut(s) 162, 294, 660
BseNI ACTGG 3 cut(s) 117, 136, 522
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 3 cut(s) 311, 412, 784
BshVI ATCGAT 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 553, 742, 844
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 1 cut(s) 736
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 50, 61, 146
BspCNI CTCAG 3 cut(s) 163, 295, 661
BspDI ATCGAT 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 368
BspPI GGATC 2 cut(s) 56, 141
BspT104I TTCGAA 1 cut(s) 876
BspTI CTTAAG 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 3 cut(s) 117, 136, 522
BssMI GATC 3 cut(s) 50, 61, 146
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 197, 496
Bst6I CTCTTC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 2 cut(s) 366, 628
BstDEI CTNAG 3 cut(s) 171, 303, 669
BstF5I GGATG 3 cut(s) 225, 657, 679
BstH2I RGCGCY 2 cut(s) 78, 171
BstHHI GCGC 2 cut(s) 77, 170
BstKTI GATC 3 cut(s) 53, 64, 149
BstMBI GATC 3 cut(s) 50, 61, 146
BstMWI GCNNNNNNNGC 2 cut(s) 611, 725
BstNI CCWGG 1 cut(s) 708
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 736
BstV1I GCAGC 3 cut(s) 311, 412, 784
Bsu15I ATCGAT 1 cut(s) 84
BsuTUI ATCGAT 1 cut(s) 84
BtsCI GGATG 3 cut(s) 225, 657, 679
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 3 cut(s) 479, 501, 568
Cac8I GCNNGC 2 cut(s) 366, 628
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 2 cut(s) 77, 170
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 2 cut(s) 343, 431
CviAII CATG 4 cut(s) 373, 648, 729, 889
CviQI GTAC 2 cut(s) 343, 431
DdeI CTNAG 3 cut(s) 171, 303, 669
DpnI GATC 3 cut(s) 52, 63, 148
DpnII GATC 3 cut(s) 50, 61, 146
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 134
Eco47III AGCGCT 2 cut(s) 76, 169
Eco57I CTGAAG 2 cut(s) 65, 197
EcoRII CCWGG 1 cut(s) 706
FaeI CATG 4 cut(s) 376, 651, 732, 892
FatI CATG 4 cut(s) 372, 647, 728, 888
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 3 cut(s) 300, 426, 798
FokI GGATG 3 cut(s) 232, 664, 686
Fsp4HI GCNGC 3 cut(s) 300, 426, 798
FspBI CTAG 1 cut(s) 365
GlaI GCGC 2 cut(s) 76, 169
GluI GCNGC 3 cut(s) 300, 426, 798
HaeII RGCGCY 2 cut(s) 78, 171
HhaI GCGC 2 cut(s) 77, 170
Hin1II CATG 4 cut(s) 376, 651, 732, 892
Hin6I GCGC 2 cut(s) 75, 168
HinP1I GCGC 2 cut(s) 75, 168
HinfI GANTC 3 cut(s) 38, 640, 656
HphI GGTGA 2 cut(s) 49, 722
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 4 cut(s) 216, 445, 645, 670
Hpy188III TCNNGA 4 cut(s) 158, 395, 455, 756
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 5 cut(s) 10, 75, 344, 551, 779
HpyCH4III ACNGT 2 cut(s) 197, 496
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 5 cut(s) 125, 499, 630, 728, 800
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 725
HpyF3I CTNAG 3 cut(s) 171, 303, 669
HpySE526I ACGT 1 cut(s) 518
Hsp92II CATG 4 cut(s) 376, 651, 732, 892
HspAI GCGC 2 cut(s) 75, 168
Kzo9I GATC 3 cut(s) 50, 61, 146
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 3 cut(s) 311, 412, 784
LweI GCATC 6 cut(s) 12, 297, 412, 664, 761, 809
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 1 cut(s) 365
MaeII ACGT 1 cut(s) 518
MalI GATC 3 cut(s) 52, 63, 148
MboI GATC 3 cut(s) 50, 61, 146
MboII GAAGA 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 3 cut(s) 11, 595, 827
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 4 cut(s) 44, 166, 210, 664
MseI TTAA 4 cut(s) 27, 581, 618, 777
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 776
MspR9I CCNGG 1 cut(s) 708
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 2 cut(s) 611, 725
NdeII GATC 3 cut(s) 50, 61, 146
NheI GCTAGC 1 cut(s) 364
NlaIII CATG 4 cut(s) 376, 651, 732, 892
NspI RCATGY 1 cut(s) 732
NspV TTCGAA 1 cut(s) 876
OliI CACNNNNGTG 1 cut(s) 494
PfeI GAWTC 2 cut(s) 38, 640
PflMI CCANNNNNTGG 1 cut(s) 844
PkrI GCNGC 3 cut(s) 301, 427, 799
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 706
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 344, 432
RsaNI GTAC 2 cut(s) 343, 431
RseI CAYNNNNRTG 1 cut(s) 494
SaqAI TTAA 4 cut(s) 27, 581, 618, 777
SatI GCNGC 3 cut(s) 300, 426, 798
Sau3AI GATC 3 cut(s) 50, 61, 146
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 1 cut(s) 736
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 6 cut(s) 12, 297, 412, 664, 761, 809
SfuI TTCGAA 1 cut(s) 876
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 467, 776
SmoI CTYRAG 2 cut(s) 467, 776
Sse9I AATT 3 cut(s) 11, 595, 827
SspMI CTAG 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 706
TaaI ACNGT 2 cut(s) 197, 496
TaiI ACGT 1 cut(s) 521
TaqI TCGA 4 cut(s) 36, 84, 607, 876
TasI AATT 3 cut(s) 11, 595, 827
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 2 cut(s) 38, 640
Tru1I TTAA 4 cut(s) 27, 581, 618, 777
Tru9I TTAA 4 cut(s) 27, 581, 618, 777
TscAI CASTG 3 cut(s) 486, 501, 568
TseI GCWGC 3 cut(s) 299, 425, 797
TspDTI ATGAA 6 cut(s) 255, 389, 648, 780, 905, 909
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 3 cut(s) 486, 501, 568
Van91I CCANNNNNTGG 1 cut(s) 844
Vha464I CTTAAG 1 cut(s) 776
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 827
XceI RCATGY 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.