AT1G05385

Photosystem II D1 precursor processing protein PSB27-H2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005678
Physical Location & Seq
Reverse (-)
1582671 .. 1583760
1090 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05385.2

Sequence Viewer

Length: 600 bp
ATGGGTTTCCTTGTAGCCGTCATGAACTTTTCTCCTACTTTGGTGCATCACCATATGAAATCGAAGCCACAATGTCAAAATGAAAAGCTTCGTCAAGGCCAAACATCATCTTTGTTTGATCGTCGTGGTTTCTTGAAATGTGTTGTTGGTGCTTCTTCGTTCATGGCCACAATTGAGTTTTCTGGATTACAAGCTCAGGCGTCAGAGGAAAAGTTAGACGAAGGAGAAGGAGTTGTTGGTGCTTTCAAGACTTTGTTTGATCCTAATGAGAGAACAAAGTCTGGCAAAGAGTTACCAAAAGCTTACTTGAAATCTGCAAGAGAGGTTGTCAAAACCATGAGGGAGTCATTGAAAGAGAACCCTAAAGACAATGCTAAGTTCAGAAGAAGTGCTGATGCTGCTAAAGAATCCATTCGTGATTATCTGAGCAATTGGAGAGGACAAAAGACTGTTGCCGGAGAAGAATCATACGTGGAGCTGGAGAATGTAATCCGCGCTTTGGCGAAATTTTACTCAAAGGCAGGTCCTTCTGCACCACTTCCTGATGAGGTTAAGACTGAGATTTTGGATGATCTTAACAAGGCTGAAGAGTTTTTGTGA

Protein Analysis

199

Amino Acids

22.27

Weight (kDa)

8.43

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_Pbs27 PF13326 23 - 145 4e-45 Photosystem II Pbs27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 512
AccII CGCG 1 cut(s) 495
AciI CCGC 1 cut(s) 493
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 506
AcyI GRCGYC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 4 cut(s) 136, 247, 310, 352
AjuI GAANNNNNNNTTGG 2 cut(s) 219, 251
AluBI AGCT 4 cut(s) 88, 194, 302, 478
AluI AGCT 4 cut(s) 88, 194, 302, 478
AlwI GGATC 1 cut(s) 254
AoxI GGCC 2 cut(s) 97, 165
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 506
ArsI GACNNNNNNTTYG 2 cut(s) 547, 579
Asp700I GAANNNNTTC 2 cut(s) 87, 411
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 524
AsuHPI GGTGA 1 cut(s) 41
AvaII GGWCC 1 cut(s) 524
BalI TGGCCA 1 cut(s) 167
BbvI GCAGC 1 cut(s) 385
BceAI ACGGC 1 cut(s) 2
BfuAI ACCTGC 1 cut(s) 512
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 524
BmsI GCATC 2 cut(s) 55, 385
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 499
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMII CTCAG 3 cut(s) 209, 416, 549
BseXI GCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 2 cut(s) 99, 167
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 1 cut(s) 499
BsnI GGCC 2 cut(s) 99, 167
Bsp143I GATC 3 cut(s) 118, 259, 571
BspACI CCGC 1 cut(s) 493
BspANI GGCC 2 cut(s) 99, 167
BspCNI CTCAG 3 cut(s) 208, 417, 550
BspFNI CGCG 1 cut(s) 495
BspHI TCATGA 1 cut(s) 21
BspMI ACCTGC 1 cut(s) 512
BspPI GGATC 1 cut(s) 254
BssMI GATC 3 cut(s) 118, 259, 571
BssNI GRCGYC 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 1 cut(s) 582
BstACI GRCGYC 1 cut(s) 200
BstBAI YACGTR 1 cut(s) 472
BstDEI CTNAG 4 cut(s) 195, 375, 425, 558
BstF5I GGATG 1 cut(s) 574
BstFNI CGCG 1 cut(s) 495
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 3 cut(s) 121, 262, 574
BstMBI GATC 3 cut(s) 118, 259, 571
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 1 cut(s) 385
BsuRI GGCC 2 cut(s) 99, 167
BtsCI GGATG 1 cut(s) 574
BveI ACCTGC 1 cut(s) 512
CciI TCATGA 1 cut(s) 21
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 1 cut(s) 524
CseI GACGC 1 cut(s) 189
CviAII CATG 3 cut(s) 22, 163, 337
CviJI RGCY 9 cut(s) 17, 67, 88, 99, 167, 194, 302, 478, 584
CviKI_1 RGCY 9 cut(s) 17, 67, 88, 99, 167, 194, 302, 478, 584
DdeI CTNAG 4 cut(s) 195, 375, 425, 558
DpnI GATC 3 cut(s) 120, 261, 573
DpnII GATC 3 cut(s) 118, 259, 571
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 524
EcoO109I RGGNCCY 1 cut(s) 524
FaeI CATG 3 cut(s) 25, 166, 340
FaiI YATR 6 cut(s) 23, 54, 56, 164, 338, 469
FatI CATG 3 cut(s) 21, 162, 336
FauNDI CATATG 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 1 cut(s) 399
GlaI GCGC 1 cut(s) 496
GluI GCNGC 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 500
HaeIII GGCC 2 cut(s) 99, 167
HapII CCGG 1 cut(s) 456
HgaI GACGC 1 cut(s) 189
HhaI GCGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 3 cut(s) 25, 166, 340
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HindIII AAGCTT 2 cut(s) 86, 300
HinfI GANTC 3 cut(s) 344, 407, 464
HpaII CCGG 1 cut(s) 456
HphI GGTGA 1 cut(s) 41
Hpy188I TCNGA 3 cut(s) 205, 383, 426
Hpy188III TCNNGA 6 cut(s) 22, 133, 183, 247, 416, 542
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 3 cut(s) 215, 221, 537
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 3 cut(s) 46, 317, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 4 cut(s) 195, 375, 425, 558
HpySE526I ACGT 1 cut(s) 471
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 3 cut(s) 25, 166, 340
HspAI GCGC 1 cut(s) 495
Kzo9I GATC 3 cut(s) 118, 259, 571
LmnI GCTCC 1 cut(s) 475
LpnPI CCDG 7 cut(s) 168, 182, 267, 464, 469, 507, 555
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 2 cut(s) 55, 385
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 1 cut(s) 291
MalI GATC 3 cut(s) 120, 261, 573
MboI GATC 3 cut(s) 118, 259, 571
MboII GAAGA 4 cut(s) 147, 396, 473, 599
MfeI CAATTG 2 cut(s) 171, 430
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 3 cut(s) 171, 430, 506
MluNI TGGCCA 1 cut(s) 167
MlyI GAGTC 1 cut(s) 353
MnlI CCTC 5 cut(s) 199, 316, 333, 431, 541
Mox20I TGGCCA 1 cut(s) 167
MroXI GAANNNNTTC 2 cut(s) 87, 411
MscI TGGCCA 1 cut(s) 167
MseI TTAA 2 cut(s) 552, 576
Msp20I TGGCCA 1 cut(s) 167
MspI CCGG 1 cut(s) 456
MunI CAATTG 2 cut(s) 171, 430
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 398
NdeI CATATG 1 cut(s) 54
NdeII GATC 3 cut(s) 118, 259, 571
NlaIII CATG 3 cut(s) 25, 166, 340
PagI TCATGA 1 cut(s) 21
PdmI GAANNNNTTC 2 cut(s) 87, 411
PfeI GAWTC 2 cut(s) 407, 464
PkrI GCNGC 1 cut(s) 400
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Ppu21I YACGTR 1 cut(s) 472
PpuMI RGGWCCY 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 524
PspPI GGNCC 1 cut(s) 524
PspPPI RGGWCCY 1 cut(s) 524
SaqAI TTAA 2 cut(s) 552, 576
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 3 cut(s) 118, 259, 571
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 1 cut(s) 353
SetI ASST 8 cut(s) 90, 196, 304, 327, 474, 480, 526, 552
SfaNI GCATC 2 cut(s) 55, 385
SinI GGWCC 1 cut(s) 524
Sse9I AATT 3 cut(s) 171, 430, 506
SsiI CCGC 1 cut(s) 493
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 1 cut(s) 474
TaqI TCGA 1 cut(s) 62
TasI AATT 3 cut(s) 171, 430, 506
TfiI GAWTC 2 cut(s) 407, 464
Tru1I TTAA 2 cut(s) 552, 576
Tru9I TTAA 2 cut(s) 552, 576
TseI GCWGC 1 cut(s) 398
TspDTI ATGAA 4 cut(s) 38, 71, 96, 151
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 506
XmnI GAANNNNTTC 2 cut(s) 87, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.