RchiOBHm_Chr4g0430981

Photosystem II D1 precursor processing protein PSB27-H2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005678
Physical Location & Seq
Reverse (-)
55354303 .. 55354653
351 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39969

Sequence Viewer

Length: 351 bp
ATGCACTCCAAGTGTGGATTACCTTCTCAAGGAGCTTCATCAACTCGCCGACTTGTAGTATATGCGGGTGCTTCTATCCTTGCGGTTCTAGCCACTGACTATGCGTTAGTGCCATTGCCTGTTTTGGCAGATGACAAGTCAAATGGTGAAGAGGAGAAGGATGGAGTTTTAGGTGCTATCCAGTCGCTGCTCGAAACCAATACGAAAACGAAGTCTGGAAGAGTGTTGCCGAAGGCATATTTGAAGACTGCAAAAGAAGTTGTGAAGACACTGCGGGAATCAGTACAGGAAGATCCCAAGGATAAAGCAAAGTTTAGACGTACTGCAGATGCAGCAAAGGAATCAATTTGA

Protein Analysis

116

Amino Acids

12.44

Weight (kDa)

9.24

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_Pbs27 PF13326 17 - 116 3.3e-15 Photosystem II Pbs27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 65, 83, 274
AclWI GGATC 1 cut(s) 287
AfaI GTAC 2 cut(s) 285, 322
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 244
AluBI AGCT 1 cut(s) 35
AluI AGCT 1 cut(s) 35
AlwI GGATC 1 cut(s) 287
AlwNI CAGNNNCTG 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 187, 332
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AsuHPI GGTGA 1 cut(s) 158
BbsI GAAGAC 2 cut(s) 251, 272
BbvI GCAGC 2 cut(s) 174, 344
BccI CCATC 1 cut(s) 155
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 2 cut(s) 188, 333
BlsI GCNGC 2 cut(s) 189, 334
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 2 cut(s) 251, 272
BpuEI CTTGAG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 297
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 2 cut(s) 174, 344
BslI CCNNNNNNNGG 1 cut(s) 29
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 3 cut(s) 65, 83, 274
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 113
BsrI ACTGG 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 1 cut(s) 292
BssT1I CCWWGG 1 cut(s) 297
Bst6I CTCTTC 2 cut(s) 144, 214
BstENI CCTNNNNNAGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 2 cut(s) 89, 332
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 174, 344
BstV2I GAAGAC 2 cut(s) 251, 272
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 2 cut(s) 93, 269
CaiI CAGNNNCTG 1 cut(s) 187
Csp6I GTAC 2 cut(s) 284, 321
CviJI RGCY 2 cut(s) 35, 92
CviKI_1 RGCY 2 cut(s) 35, 92
CviQI GTAC 2 cut(s) 284, 321
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 144, 214
EarI CTCTTC 2 cut(s) 144, 214
Eco130I CCWWGG 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 297
FaiI YATR 4 cut(s) 61, 63, 102, 238
FauI CCCGC 2 cut(s) 58, 267
Fnu4HI GCNGC 2 cut(s) 188, 333
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 188, 333
FspBI CTAG 1 cut(s) 89
GluI GCNGC 2 cut(s) 188, 333
HinfI GANTC 2 cut(s) 278, 341
HphI GGTGA 1 cut(s) 158
Hpy188III TCNNGA 1 cut(s) 216
HpyAV CCTTC 3 cut(s) 33, 151, 226
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 4 cut(s) 4, 251, 326, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 332
HpySE526I ACGT 1 cut(s) 319
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 4 cut(s) 132, 194, 201, 272
Lsp1109I GCAGC 2 cut(s) 174, 344
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 319
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 5 cut(s) 161, 231, 256, 277, 302
MflI RGATCY 1 cut(s) 292
MluCI AATT 1 cut(s) 345
MnlI CCTC 1 cut(s) 145
MwoI GCNNNNNNNGC 2 cut(s) 89, 332
NdeII GATC 1 cut(s) 292
PfeI GAWTC 2 cut(s) 278, 341
PkrI GCNGC 2 cut(s) 189, 334
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 2 cut(s) 285, 322
RsaNI GTAC 2 cut(s) 284, 321
SatI GCNGC 2 cut(s) 188, 333
Sau3AI GATC 1 cut(s) 292
SetI ASST 4 cut(s) 25, 37, 175, 322
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 1 cut(s) 345
SsiI CCGC 3 cut(s) 65, 83, 274
SspMI CTAG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 297
TaiI ACGT 1 cut(s) 322
TaqI TCGA 1 cut(s) 192
TasI AATT 1 cut(s) 345
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 2 cut(s) 278, 341
TscAI CASTG 2 cut(s) 100, 276
TseI GCWGC 2 cut(s) 187, 332
TspDTI ATGAA 1 cut(s) 27
TspRI CASTG 2 cut(s) 100, 276
XagI CCTNNNNNAGG 1 cut(s) 27
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.