Rh4DG199500

Photosystem II D1 precursor processing protein PSB27-H2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005678
Physical Location & Seq
Forward (+)
38846605 .. 38847168
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG199500.1

Sequence Viewer

Length: 564 bp
ATGATATGCTTCTCTCCTGTAACAGATAAAATGCACTCCAGGTGTGCATTACCTTCTGAAGGAGCTTCATCAACTCGCCGACTTGTAGTATATGCGGGTGCTTCTATCCTTGCGGTTCTAGCAACTAACTATGGGTTAGCGCCATTGCCTGTTTTGGCAGATGACAAATCAAATGGTGAAGAGGAGAGGGATGGAGTTTTAGGTGCTATCCAGTCGCTGCTCGAAACCAATACGAAAACTGAGTCTGGAAGAGTGTTGCCAAAGGCATATTTGAAGACTGCAAAAGAAGTTGTGAAGACACTGCGGGAATCAGTACAGGAAGATCCCAAGGATAATGCAAAGTTTAGACGTACTGCAGATGCAGCAAAGGAATCAATTCGAGAGTATTTGAGCAATTGGAGGGGACAGCCAACGGTGTCTCAAGAGGAATCTTATGTCGAGTTAGAGAAAGCAATAAGATCATTGGCCGGCTTTTACTCGAAGGCAGGACCATCTGCCCCATTGCCTGAAGAAGTGAAGTCAGAAATATTGAGGGATTTAGATACAGCTGAAAAGTTTTTATGA

Protein Analysis

187

Amino Acids

20.49

Weight (kDa)

5.65

Isoelectric Point (pI)

58.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_Pbs27 PF13326 27 - 187 1.7e-46 Photosystem II Pbs27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 95, 113, 304
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 465
AcuI CTGAAG 2 cut(s) 78, 528
AfaI GTAC 2 cut(s) 315, 352
AfiI CCNNNNNNNGG 1 cut(s) 59
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 1 cut(s) 38
AluBI AGCT 2 cut(s) 65, 548
AluI AGCT 2 cut(s) 65, 548
Alw26I GTCTC 1 cut(s) 423
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 1 cut(s) 465
ApeKI GCWGC 2 cut(s) 217, 362
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 488
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 488
BbsI GAAGAC 2 cut(s) 281, 302
BbvI GCAGC 2 cut(s) 204, 374
BccI CCATC 2 cut(s) 185, 499
BciT130I CCWGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 2 cut(s) 218, 363
BlsI GCNGC 2 cut(s) 219, 364
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 1 cut(s) 488
BmgT120I GGNCC 1 cut(s) 488
BmrFI CCNGG 1 cut(s) 40
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 2 cut(s) 281, 302
BpmI CTGGAG 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 211
Bse3DI GCAATG 2 cut(s) 143, 500
BseBI CCWGG 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMI GCAATG 2 cut(s) 143, 500
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 204, 374
BshFI GGCC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 468
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 1 cut(s) 467
Bsp143I GATC 2 cut(s) 322, 458
BspACI CCGC 3 cut(s) 95, 113, 304
BspANI GGCC 1 cut(s) 467
BspCNI CTCAG 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 1 cut(s) 317
BsrDI GCAATG 2 cut(s) 143, 500
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 211
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 2 cut(s) 322, 458
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 40
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 2 cut(s) 174, 244
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 196
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 2 cut(s) 325, 461
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 2 cut(s) 322, 458
BstMWI GCNNNNNNNGC 2 cut(s) 119, 362
BstNI CCWGG 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 38
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 204, 374
BstV2I GAAGAC 2 cut(s) 281, 302
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 1 cut(s) 467
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 1 cut(s) 469
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 488
Csp6I GTAC 2 cut(s) 314, 351
CviJI RGCY 5 cut(s) 65, 409, 467, 471, 548
CviKI_1 RGCY 5 cut(s) 65, 409, 467, 471, 548
CviQI GTAC 2 cut(s) 314, 351
DdeI CTNAG 1 cut(s) 240
DpnI GATC 2 cut(s) 324, 460
DpnII GATC 2 cut(s) 322, 458
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 174, 244
EarI CTCTTC 2 cut(s) 174, 244
Eco130I CCWWGG 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 488
Eco57I CTGAAG 2 cut(s) 78, 528
EcoNI CCTNNNNNAGG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaiI YATR 7 cut(s) 7, 91, 93, 132, 268, 435, 562
FaqI GGGAC 1 cut(s) 417
FauI CCCGC 2 cut(s) 88, 297
Fnu4HI GCNGC 2 cut(s) 218, 363
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 218, 363
FspBI CTAG 1 cut(s) 119
GlaI GCGC 1 cut(s) 141
GluI GCNGC 2 cut(s) 218, 363
GsuI CTGGAG 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 1 cut(s) 467
HapII CCGG 1 cut(s) 468
HhaI GCGC 1 cut(s) 142
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 4 cut(s) 242, 308, 371, 428
HpaII CCGG 1 cut(s) 468
HphI GGTGA 1 cut(s) 188
Hpy188I TCNGA 2 cut(s) 58, 523
Hpy188III TCNNGA 3 cut(s) 246, 380, 422
HpyAV CCTTC 3 cut(s) 53, 63, 475
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 6 cut(s) 34, 47, 281, 338, 356, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 362
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 1 cut(s) 349
HspAI GCGC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 467
KroNI GCCGGC 1 cut(s) 469
Kzo9I GATC 2 cut(s) 322, 458
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 2 cut(s) 204, 374
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 324, 460
MboI GATC 2 cut(s) 322, 458
MboII GAAGA 6 cut(s) 191, 261, 286, 307, 332, 521
MfeI CAATTG 1 cut(s) 394
MflI RGATCY 1 cut(s) 322
MluCI AATT 2 cut(s) 375, 394
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 5 cut(s) 175, 180, 393, 418, 525
MroNI GCCGGC 1 cut(s) 467
MroXI GAANNNNTTC 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 40
MunI CAATTG 1 cut(s) 394
MvaI CCWGG 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 119, 362
NaeI GCCGGC 1 cut(s) 469
NdeII GATC 2 cut(s) 322, 458
NgoMIV GCCGGC 1 cut(s) 467
PdiI GCCGGC 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 308, 371, 428
PkrI GCNGC 2 cut(s) 219, 364
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 38
PspGI CCWGG 1 cut(s) 38
PspPI GGNCC 1 cut(s) 488
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 2 cut(s) 315, 352
RsaNI GTAC 2 cut(s) 314, 351
SatI GCNGC 2 cut(s) 218, 363
Sau3AI GATC 2 cut(s) 322, 458
Sau96I GGNCC 1 cut(s) 488
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 40
SetI ASST 6 cut(s) 44, 55, 67, 205, 352, 550
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
Sse9I AATT 2 cut(s) 375, 394
SsiI CCGC 3 cut(s) 95, 113, 304
SspI AATATT 1 cut(s) 528
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 1 cut(s) 352
TaqI TCGA 4 cut(s) 222, 379, 438, 479
TasI AATT 2 cut(s) 375, 394
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 3 cut(s) 308, 371, 428
TscAI CASTG 1 cut(s) 306
TseI GCWGC 2 cut(s) 217, 362
TspDTI ATGAA 1 cut(s) 57
TspRI CASTG 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 488
XagI CCTNNNNNAGG 1 cut(s) 57
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.