RLG00000034653

Photosystem II D1 precursor processing protein PSB27-H2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005678
Physical Location & Seq
Forward (+)
47201455 .. 47202113
659 bp
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UTR
Exon/CDS
Intron
RLM00000034653

Sequence Viewer

Length: 462 bp
ATGGCTGCATCTGTTTATCTTGCTCCAGAGCTCTTCCCTCTTGTCATTTGCAGGACAGGGAAGAATAGAATGAGACCTAATAAAGAGTATAAAATGCACTCCAGGTGTGCATTACCTTTTCAAGGAGCTTCATCAACTCGCCGACTTGTAGTATATGCGGGTGCTTCTATCCTTGTGGTTCTAGCCACTGACTATGGGTTAGTGCCATTGCCTGTTTTGGCAGATGACAAGTCAAATGGTGAAGAGGAGAAGGATGGAGTTTTAGGTGCTATCCAGTCGTTGCTCGAAACCAATACGAAAACAAAGTCTAGAAGAGTGTTGCCAAAGGCATATTTGAAGACTGCAAAAGAAGTTGTGAAGACACTGCGGGAATCAGTACAGGAAGATCCCAAGGATAATGCAAAGTTTAGACGTACTGCAGATGCAGCAAAGGAATCAATTCTAGAGTATTTGAGCAATTAG

Protein Analysis

154

Amino Acids

16.94

Weight (kDa)

9.46

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_Pbs27 PF13326 48 - 152 5.2e-16 Photosystem II Pbs27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 158, 367
AclWI GGATC 1 cut(s) 380
AfaI GTAC 2 cut(s) 378, 415
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 2 cut(s) 122, 337
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 2 cut(s) 31, 128
AluI AGCT 2 cut(s) 31, 128
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 5, 425
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
Asp700I GAANNNNTTC 1 cut(s) 438
AsuHPI GGTGA 1 cut(s) 251
BanII GRGCYC 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 344, 365
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 437
BccI CCATC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 3 cut(s) 182, 309, 443
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 2 cut(s) 6, 426
BlsI GCNGC 2 cut(s) 7, 427
Bme1390I CCNGG 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 17, 412
BpiI GAAGAC 2 cut(s) 344, 365
BpmI CTGGAG 2 cut(s) 9, 85
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 390
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMI GCAATG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 2 cut(s) 158, 367
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 1 cut(s) 380
BspQI GCTCTTC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 1 cut(s) 385
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 103
Bst6I CTCTTC 3 cut(s) 38, 237, 307
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 259
BstKTI GATC 1 cut(s) 388
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 437
BstV2I GAAGAC 2 cut(s) 344, 365
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BtsCI GGATG 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 2 cut(s) 186, 362
Csp6I GTAC 2 cut(s) 377, 414
CviJI RGCY 4 cut(s) 5, 31, 128, 185
CviKI_1 RGCY 4 cut(s) 5, 31, 128, 185
CviQI GTAC 2 cut(s) 377, 414
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eam1104I CTCTTC 3 cut(s) 38, 237, 307
EarI CTCTTC 3 cut(s) 38, 237, 307
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 390
FaiI YATR 5 cut(s) 90, 154, 156, 195, 331
FauI CCCGC 2 cut(s) 151, 360
Fnu4HI GCNGC 2 cut(s) 6, 426
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 2 cut(s) 6, 426
FspBI CTAG 3 cut(s) 182, 309, 443
GluI GCNGC 2 cut(s) 6, 426
GsuI CTGGAG 2 cut(s) 9, 85
HinfI GANTC 2 cut(s) 371, 434
HphI GGTGA 1 cut(s) 251
Hpy188III TCNNGA 3 cut(s) 26, 309, 443
HpyAV CCTTC 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 8 cut(s) 8, 51, 97, 110, 344, 401, 419, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpySE526I ACGT 1 cut(s) 412
Kzo9I GATC 1 cut(s) 385
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 2 cut(s) 28, 125
LpnPI CCDG 8 cut(s) 37, 39, 42, 88, 115, 225, 287, 365
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 2 cut(s) 17, 412
MaeI CTAG 3 cut(s) 182, 309, 443
MaeII ACGT 1 cut(s) 412
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 7 cut(s) 25, 73, 254, 324, 349, 370, 395
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 2 cut(s) 438, 457
MnlI CCTC 2 cut(s) 48, 238
MroXI GAANNNNTTC 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 425
NdeII GATC 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 2 cut(s) 371, 434
PkrI GCNGC 2 cut(s) 7, 427
Psp124BI GAGCTC 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 2 cut(s) 378, 415
RsaNI GTAC 2 cut(s) 377, 414
SacI GAGCTC 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 38
SatI GCNGC 2 cut(s) 6, 426
Sau3AI GATC 1 cut(s) 385
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 33, 79, 107, 118, 130, 268, 415
SfaNI GCATC 2 cut(s) 17, 412
SfcI CTRYAG 1 cut(s) 417
Sse9I AATT 2 cut(s) 438, 457
SsiI CCGC 2 cut(s) 158, 367
SspMI CTAG 3 cut(s) 182, 309, 443
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 390
TaiI ACGT 1 cut(s) 415
TaqI TCGA 1 cut(s) 285
TasI AATT 2 cut(s) 438, 457
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 2 cut(s) 371, 434
TscAI CASTG 2 cut(s) 193, 369
TseI GCWGC 2 cut(s) 5, 425
TspDTI ATGAA 1 cut(s) 120
TspRI CASTG 2 cut(s) 193, 369
XagI CCTNNNNNAGG 1 cut(s) 120
XbaI TCTAGA 2 cut(s) 308, 442
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 182, 309, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.