MD16G1193700.v1.1

Photosystem II D1 precursor processing protein PSB27-H2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005678
Physical Location & Seq
Reverse (-)
17191946 .. 17193672
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1193700.v1.1.491

Sequence Viewer

Length: 633 bp
ATGAAGAATATGGCTCTTCTTGCAGAAAAGATCTGCCTCCCCGTACTTTTCAGGACGAGGGAAAATAATACCTTCAAACCTGCCAAACAGTATAAAGTGCGGTCCAGGTGTGAGCTACCCTCTCAAGAAGCTTCATCAAGTCGCCGACATTTTGTAACGTGTGCTGGTGCTTCGATTGCAACGGTTTTAACCATCAGCAATGTCTTAGCACCATCGCCGGTTTGGGCCGATGACAAGTCAAATGGCGAAGAGGAAAATGATGGAGTTATAGGTGCTCTTAAAACACTGTTCGATCCCAATGAGAAAACAAAGTCCGGAAAAGTGTTGCCAAGGGCTTACTTGAAGTCGGCAAAAGAGGTTGTGAAGACACTGCGTGAATCACTGAACGAAGACACCAAGGATAATGCTAAATTTCGACGAACTGCAGATGCAGCGAAGGAATCGATTCGAGAGTATTTGAGCACTTGGAGAGGACAACAAGAGGTGGCTCAAGAGGAATCTTACGTTGAGATAGAGAAAGCAATAAGATCGTTGGCCGGATTTTACTCCAAGGCAGGGCCATCTGCTCCACTGCCTGAACAAGTCAAGTCTGAAATATTGAATGACTTGAACACAGCTGAAGAGTTTTTGTGA

Protein Analysis

211

Amino Acids

23.4

Weight (kDa)

8.34

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII_Pbs27 PF13326 47 - 210 1e-49 Photosystem II Pbs27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 88
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 1 cut(s) 100
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 1 cut(s) 410
AdeI CACNNNGTG 1 cut(s) 374
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 555
AflIII ACRYGT 1 cut(s) 158
AgsI TTSAA 4 cut(s) 76, 343, 601, 610
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 3 cut(s) 115, 131, 617
AluI AGCT 3 cut(s) 115, 131, 617
Alw21I GWGCWC 2 cut(s) 277, 464
AlwI GGATC 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 314
AoxI GGCC 3 cut(s) 225, 534, 557
ApeKI GCWGC 1 cut(s) 431
ApoI RAATTY 1 cut(s) 410
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 3 cut(s) 102, 225, 557
AvaII GGWCC 1 cut(s) 102
BbsI GAAGAC 2 cut(s) 371, 396
Bbv12I GWGCWC 2 cut(s) 277, 464
BbvI GCAGC 1 cut(s) 443
BccI CCATC 4 cut(s) 200, 220, 254, 568
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 1 cut(s) 106
BfmI CTRYAG 1 cut(s) 423
BfuAI ACCTGC 1 cut(s) 88
BglII AGATCT 1 cut(s) 30
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 3 cut(s) 102, 225, 557
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 2 cut(s) 371, 396
BplI GAGNNNNNCTC 2 cut(s) 104, 136
BpuEI CTTGAG 2 cut(s) 108, 474
Bsa29I ATCGAT 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 329, 396, 549
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse118I RCCGGY 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 205
BseAI TCCGGA 1 cut(s) 314
BseBI CCWGG 1 cut(s) 106
BseCI ATCGAT 1 cut(s) 443
BseDI CCNNGG 3 cut(s) 329, 396, 549
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMI GCAATG 1 cut(s) 205
BseXI GCAGC 1 cut(s) 443
BshFI GGCC 3 cut(s) 227, 536, 559
BshVI ATCGAT 1 cut(s) 443
BsiHKAI GWGCWC 2 cut(s) 277, 464
BsiSI CCGG 3 cut(s) 218, 315, 537
BslI CCNNNNNNNGG 1 cut(s) 555
BsnI GGCC 3 cut(s) 227, 536, 559
Bsp1286I GDGCHC 2 cut(s) 277, 464
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 3 cut(s) 30, 292, 527
BspACI CCGC 1 cut(s) 100
BspANI GGCC 3 cut(s) 227, 536, 559
BspDI ATCGAT 1 cut(s) 443
BspEI TCCGGA 1 cut(s) 314
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 88
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 329, 396, 549
BssMI GATC 3 cut(s) 30, 292, 527
BssT1I CCWWGG 3 cut(s) 329, 396, 549
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 3 cut(s) 90, 184, 288
Bst6I CTCTTC 3 cut(s) 21, 243, 615
BstDEI CTNAG 1 cut(s) 205
BstKTI GATC 3 cut(s) 33, 295, 530
BstMBI GATC 3 cut(s) 30, 292, 527
BstMWI GCNNNNNNNGC 3 cut(s) 20, 176, 431
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 443
BstV2I GAAGAC 2 cut(s) 371, 396
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
Bsu15I ATCGAT 1 cut(s) 443
BsuRI GGCC 3 cut(s) 227, 536, 559
BsuTUI ATCGAT 1 cut(s) 443
BtgZI GCGATG 1 cut(s) 198
BtsI GCAGTG 2 cut(s) 368, 569
BtsIMutI CAGTG 4 cut(s) 284, 368, 380, 569
BveI ACCTGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 102, 225, 557
ClaI ATCGAT 1 cut(s) 443
Csp6I GTAC 1 cut(s) 44
CviJI RGCY 9 cut(s) 14, 115, 131, 227, 335, 488, 536, 559, 617
CviKI_1 RGCY 9 cut(s) 14, 115, 131, 227, 335, 488, 536, 559, 617
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 1 cut(s) 205
DpnI GATC 3 cut(s) 32, 294, 529
DpnII GATC 3 cut(s) 30, 292, 527
DraIII CACNNNGTG 1 cut(s) 374
EaeI YGGCCR 1 cut(s) 534
Eam1104I CTCTTC 3 cut(s) 21, 243, 615
EarI CTCTTC 3 cut(s) 21, 243, 615
Eco130I CCWWGG 3 cut(s) 329, 396, 549
Eco47I GGWCC 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 3 cut(s) 329, 396, 549
ErhI CCWWGG 3 cut(s) 329, 396, 549
FaiI YATR 3 cut(s) 11, 93, 269
Fnu4HI GCNGC 1 cut(s) 432
Fsp4HI GCNGC 1 cut(s) 432
GluI GCNGC 1 cut(s) 432
HaeIII GGCC 3 cut(s) 227, 536, 559
HapII CCGG 3 cut(s) 218, 315, 537
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 4 cut(s) 377, 440, 445, 497
HpaII CCGG 3 cut(s) 218, 315, 537
Hpy188I TCNGA 1 cut(s) 592
Hpy188III TCNNGA 5 cut(s) 52, 125, 315, 449, 491
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 2 cut(s) 82, 430
HpyCH4III ACNGT 3 cut(s) 90, 184, 288
HpyCH4IV ACGT 2 cut(s) 158, 504
HpyCH4V TGCA 4 cut(s) 23, 179, 425, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 176, 431
HpyF3I CTNAG 1 cut(s) 205
HpySE526I ACGT 2 cut(s) 158, 504
Kpn2I TCCGGA 1 cut(s) 314
Kzo9I GATC 3 cut(s) 30, 292, 527
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 1 cut(s) 418
MaeII ACGT 2 cut(s) 158, 504
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 32, 294, 529
MboI GATC 3 cut(s) 30, 292, 527
MboII GAAGA 6 cut(s) 8, 16, 260, 376, 401, 632
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 277, 464
MluCI AATT 1 cut(s) 410
MnlI CCTC 8 cut(s) 47, 51, 130, 244, 349, 464, 475, 487
MroI TCCGGA 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 2 cut(s) 188, 279
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 3 cut(s) 218, 315, 537
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 3 cut(s) 20, 176, 431
NdeII GATC 3 cut(s) 30, 292, 527
PciSI GCTCTTC 1 cut(s) 21
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 4 cut(s) 377, 440, 445, 497
PkrI GCNGC 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspPI GGNCC 3 cut(s) 102, 225, 557
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 188, 279
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 3 cut(s) 30, 292, 527
Sau96I GGNCC 3 cut(s) 102, 225, 557
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 2 cut(s) 277, 464
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 123, 489
SmoI CTYRAG 2 cut(s) 123, 489
Sse9I AATT 1 cut(s) 410
SsiI CCGC 1 cut(s) 100
SspI AATATT 1 cut(s) 597
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 3 cut(s) 329, 396, 549
TaaI ACNGT 3 cut(s) 90, 184, 288
TaiI ACGT 2 cut(s) 161, 507
TaqI TCGA 5 cut(s) 173, 291, 415, 443, 448
TasI AATT 1 cut(s) 410
TfiI GAWTC 4 cut(s) 377, 440, 445, 497
Tru1I TTAA 2 cut(s) 188, 279
Tru9I TTAA 2 cut(s) 188, 279
TscAI CASTG 4 cut(s) 291, 375, 387, 576
TseI GCWGC 1 cut(s) 431
TspDTI ATGAA 2 cut(s) 17, 123
TspRI CASTG 4 cut(s) 291, 375, 387, 576
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.