AT1G05750

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012282
Physical Location & Seq
Forward (+)
1721509 .. 1724734
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05750.2

Sequence Viewer

Length: 1503 bp
ATGGGTCTCCTTCCCGTCGTCGGAATTACTTCGCCGGCGCTCATCACCCACAAAAATCACGCCAATCCAAAGATACAGAGACATAATCAATCTACCTCCGAGACCACTGTTTCATGGACTTCTCGCATCAATCTCCTCACGCGCAATGGTCGATTAGCGGAGGCAGCGAAGGAATTCTCCGATATGACACTCGCCGGCGTAGAGCCTAACCACATCACTTTCATAGCTCTACTCTCCGGGTGTGGTGATTTTACCTCCGGAAGTGAAGCCTTGGGCGATTTGCTTCATGGGTATGCTTGTAAACTTGGTCTTGATAGAAACCATGTCATGGTTGGCACCGCAATTATCGGCATGTACTCCAAACGCGGCCGTTTTAAGAAGGCTAGATTGGTTTTTGATTACATGGAAGATAAAAATTCGGTTACTTGGAATACAATGATCGATGGGTACATGAGAAGTGGTCAAGTCGATAACGCTGCTAAGATGTTCGACAAAATGCCTGAACGAGACTTGATTTCTTGGACGGCTATGATAAATGGCTTTGTTAAGAAAGGTTATCAAGAGGAAGCTTTGTTATGGTTCCGTGAAATGCAGATTTCTGGAGTAAAACCAGATTACGTTGCTATTATTGCTGCTCTTAACGCTTGCACAAACCTTGGTGCTCTCTCATTTGGATTATGGGTACATCGTTATGTTTTGAGTCAGGATTTCAAGAACAATGTGAGGGTGAGTAATTCACTGATCGATTTGTATTGTCGATGCGGGTGTGTGGAGTTTGCTCGACAAGTTTTTTACAACATGGAGAAACGAACCGTAGTTTCGTGGAATTCAGTCATTGTTGGTTTTGCTGCTAATGGAAATGCACATGAATCATTAGTCTACTTCAGGAAAATGCAAGAGAAAGGCTTTAAACCTGACGCAGTCACTTTCACTGGGGCGCTTACCGCGTGTAGCCATGTAGGTCTAGTTGAAGAAGGTCTTCGATATTTTCAAATTATGAAATGTGATTACAGAATCTCGCCTCGAATTGAGCATTATGGATGCTTAGTGGATTTGTATAGTCGGGCTGGGAGATTGGAAGACGCTTTGAAGTTGGTGCAGAGCATGCCAATGAAGCCAAATGAAGTTGTGATTGGATCGTTGCTTGCAGCTTGCAGCAATCATGGGAACAATATCGTCTTAGCAGAGAGGCTGATGAAGCATCTTACCGACCTGAATGTGAAAAGCCATTCAAATTATGTGATTCTCTCGAATATGTATGCTGCTGATGGAAAATGGGAAGGAGCAAGTAAGATGAGAAGGAAGATGAAGGGTCTCGGTCTAAAAAAGCAGCCTGGGTTTAGTTCGATAGAGATTGATGATTGCATGCATGTGTTCATGGCCGGTGACAATGCCCATGTTGAGACCACTTATATCCGCGAGGTCCTGGAGCTTATTTCTTCTGATTTGCGATTACAGGGCTGTGTAGTTGAAACCCTTGCTGGTGATCTCCTCAATGCTTGA

Protein Analysis

500

Amino Acids

55.78

Weight (kDa)

8.59

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 35 - 81 2.5e-08 PPR repeat family
PPR_2 PF13041 138 - 169 2.4e-09 PPR repeat family
PPR_1 PF12854 139 - 166 3.6e-08 PPR repeat
PPR PF01535 141 - 170 5.2e-09 PPR repeat
PPR_2 PF13041 170 - 215 1.6e-07 PPR repeat family
PPR PF01535 172 - 202 7e-06 PPR repeat
PPR_2 PF13041 271 - 318 1.2e-09 PPR repeat family
PPR PF01535 273 - 303 8.1e-06 PPR repeat
E_motif PF20431 390 - 451 2.1e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05750 AT1G05750
fragaria_vesca FvH4_3g26660
malus_domestica MD00G1049500.v1.1 MD00G1071600.v1.1 MD03G1154500.v1.1
prunus_persica Prupe.6G143700_v2.0.a1
pyrus_communis pycom03g10830 pycom11g14680
rosa_chinensis RchiOBHm_Chr5g0048131 RchiOBHm_Chr5g0048161
rosa_laevigata RLG00000034500
rosa_multiflora Rmu_sc0001408.1_g000020
rosa_roxburghii Rroxscaffold_1G00033760
rosa_rugosa Rorug05G0236900
rosa_samantha Rh5AG316000 Rh5BG326400 Rh5CG352900
rosa_wichuraiana Rw5G029620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 335
AccB7I CCANNNNNTGG 1 cut(s) 328
AccI GTMKAC 1 cut(s) 879
AccII CGCG 4 cut(s) 142, 366, 947, 1419
AccIII TCCGGA 1 cut(s) 257
AciI CCGC 6 cut(s) 158, 339, 366, 762, 945, 1417
AclWI GGATC 1 cut(s) 1144
AcoI YGGCCR 2 cut(s) 367, 1380
AcsI RAATTY 3 cut(s) 173, 415, 826
AcuI CTGAAG 1 cut(s) 868
AfaI GTAC 3 cut(s) 356, 449, 684
AfiI CCNNNNNNNGG 2 cut(s) 20, 328
AgsI TTSAA 6 cut(s) 712, 971, 992, 1090, 1233, 1472
AjnI CCWGG 2 cut(s) 1333, 1425
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 1116, 1148
AluBI AGCT 4 cut(s) 227, 569, 1151, 1432
AluI AGCT 4 cut(s) 227, 569, 1151, 1432
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 6 cut(s) 11, 73, 95, 501, 1319, 1397
AlwI GGATC 1 cut(s) 1144
Aor13HI TCCGGA 1 cut(s) 257
AoxI GGCC 2 cut(s) 367, 1380
ApeKI GCWGC 8 cut(s) 164, 476, 632, 848, 1148, 1155, 1262, 1330
ApoI RAATTY 3 cut(s) 173, 415, 826
Asp700I GAANNNNTTC 3 cut(s) 28, 173, 978
AspLEI GCGC 3 cut(s) 40, 144, 940
AspS9I GGNCC 1 cut(s) 1423
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 5 cut(s) 37, 257, 739, 1397, 1496
AvaII GGWCC 1 cut(s) 1423
BanI GGYRCC 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 971, 1086
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 8 cut(s) 176, 463, 619, 835, 1160, 1167, 1249, 1342
BccI CCATC 2 cut(s) 437, 1262
BceAI ACGGC 2 cut(s) 354, 540
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 2 cut(s) 1335, 1427
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 6 cut(s) 11, 73, 95, 501, 1319, 1397
BfaI CTAG 2 cut(s) 384, 965
BfoI RGCGCY 2 cut(s) 41, 941
BisI GCNGC 9 cut(s) 165, 367, 477, 633, 849, 1149, 1156, 1263, 1331
BlsI GCNGC 9 cut(s) 166, 368, 478, 634, 850, 1150, 1157, 1264, 1332
Bme1390I CCNGG 3 cut(s) 238, 1335, 1427
Bme18I GGWCC 1 cut(s) 1423
BmgT120I GGNCC 1 cut(s) 1423
BmiI GGNNCC 2 cut(s) 337, 581
BmrFI CCNGG 3 cut(s) 238, 1335, 1427
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 4 cut(s) 135, 749, 1031, 1210
BmuI ACTGGG 1 cut(s) 942
BpiI GAAGAC 2 cut(s) 971, 1086
BpmI CTGGAG 2 cut(s) 621, 1448
BpuMI CCSGG 1 cut(s) 238
Bsa29I ATCGAT 2 cut(s) 441, 744
BsaI GGTCTC 4 cut(s) 11, 95, 1319, 1397
BsaJI CCNNGG 3 cut(s) 270, 655, 1334
BsaWI WCCGGW 1 cut(s) 257
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 328
Bse118I RCCGGY 3 cut(s) 34, 194, 1382
Bse1I ACTGG 1 cut(s) 937
Bse3DI GCAATG 1 cut(s) 151
BseAI TCCGGA 1 cut(s) 257
BseBI CCWGG 2 cut(s) 1335, 1427
BseCI ATCGAT 2 cut(s) 441, 744
BseDI CCNNGG 3 cut(s) 270, 655, 1334
BseGI GGATG 1 cut(s) 1046
BseLI CCNNNNNNNGG 2 cut(s) 20, 328
BseMI GCAATG 1 cut(s) 151
BseNI ACTGG 1 cut(s) 937
BseRI GAGGAG 2 cut(s) 125, 1481
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 8 cut(s) 176, 463, 619, 835, 1160, 1167, 1249, 1342
BseYI CCCAGC 1 cut(s) 1067
BsgI GTGCAG 1 cut(s) 1118
Bsh1236I CGCG 4 cut(s) 142, 366, 947, 1419
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 369, 1382
BshNI GGYRCC 1 cut(s) 335
BshVI ATCGAT 2 cut(s) 441, 744
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 5 cut(s) 35, 195, 237, 258, 1383
BslI CCNNNNNNNGG 2 cut(s) 20, 328
BsmAI GTCTC 6 cut(s) 11, 73, 95, 501, 1319, 1397
BsnI GGCC 2 cut(s) 369, 1382
Bso31I GGTCTC 4 cut(s) 11, 95, 1319, 1397
Bsp1286I GDGCHC 1 cut(s) 664
Bsp13I TCCGGA 1 cut(s) 257
Bsp143I GATC 4 cut(s) 438, 741, 1136, 1486
BspACI CCGC 6 cut(s) 158, 339, 366, 762, 945, 1417
BspANI GGCC 2 cut(s) 369, 1382
BspDI ATCGAT 2 cut(s) 441, 744
BspEI TCCGGA 1 cut(s) 257
BspFNI CGCG 4 cut(s) 142, 366, 947, 1419
BspLI GGNNCC 2 cut(s) 337, 581
BspPI GGATC 1 cut(s) 1144
BspT107I GGYRCC 1 cut(s) 335
BspTNI GGTCTC 4 cut(s) 11, 95, 1319, 1397
BsrDI GCAATG 1 cut(s) 151
BsrFI RCCGGY 3 cut(s) 34, 194, 1382
BsrI ACTGG 1 cut(s) 937
BssAI RCCGGY 3 cut(s) 34, 194, 1382
BssECI CCNNGG 3 cut(s) 270, 655, 1334
BssMI GATC 4 cut(s) 438, 741, 1136, 1486
BssT1I CCWWGG 2 cut(s) 270, 655
Bst2UI CCWGG 2 cut(s) 1335, 1427
Bst4CI ACNGT 2 cut(s) 109, 814
BstAPI GCANNNNNTGC 1 cut(s) 1105
BstC8I GCNNGC 7 cut(s) 36, 196, 646, 1106, 1146, 1153, 1367
BstDEI CTNAG 3 cut(s) 480, 1045, 1180
BstF5I GGATG 1 cut(s) 1046
BstFNI CGCG 4 cut(s) 142, 366, 947, 1419
BstH2I RGCGCY 2 cut(s) 41, 941
BstHHI GCGC 3 cut(s) 40, 144, 940
BstKTI GATC 4 cut(s) 441, 744, 1139, 1489
BstMAI GTCTC 6 cut(s) 11, 73, 95, 501, 1319, 1397
BstMBI GATC 4 cut(s) 438, 741, 1136, 1486
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 7 cut(s) 164, 629, 641, 944, 1105, 1114, 1198
BstNI CCWGG 2 cut(s) 1335, 1427
BstNSI RCATGY 4 cut(s) 355, 1108, 1369, 1373
BstSCI CCNGG 3 cut(s) 236, 1333, 1425
BstUI CGCG 4 cut(s) 142, 366, 947, 1419
BstV1I GCAGC 8 cut(s) 176, 463, 619, 835, 1160, 1167, 1249, 1342
BstV2I GAAGAC 2 cut(s) 971, 1086
BstZI CGGCCG 1 cut(s) 367
Bsu15I ATCGAT 2 cut(s) 441, 744
BsuRI GGCC 2 cut(s) 369, 1382
BsuTUI ATCGAT 2 cut(s) 441, 744
BtsCI GGATG 1 cut(s) 1046
BtsIMutI CAGTG 3 cut(s) 105, 737, 930
Cac8I GCNNGC 7 cut(s) 36, 196, 646, 1106, 1146, 1153, 1367
CfoI GCGC 3 cut(s) 40, 144, 940
Cfr10I RCCGGY 3 cut(s) 34, 194, 1382
Cfr13I GGNCC 1 cut(s) 1423
ClaI ATCGAT 2 cut(s) 441, 744
CseI GACGC 2 cut(s) 926, 1091
Csp6I GTAC 3 cut(s) 355, 448, 683
CviQI GTAC 3 cut(s) 355, 448, 683
DdeI CTNAG 3 cut(s) 480, 1045, 1180
DpnI GATC 4 cut(s) 440, 743, 1138, 1488
DpnII GATC 4 cut(s) 438, 741, 1136, 1486
DraI TTTAAA 1 cut(s) 910
EaeI YGGCCR 2 cut(s) 367, 1380
EagI CGGCCG 1 cut(s) 367
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 270, 655
Eco31I GGTCTC 4 cut(s) 11, 95, 1319, 1397
Eco47I GGWCC 1 cut(s) 1423
Eco52I CGGCCG 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 868
EcoO109I RGGNCCY 1 cut(s) 1423
EcoRI GAATTC 2 cut(s) 173, 826
EcoRII CCWGG 2 cut(s) 1333, 1425
EcoT14I CCWWGG 2 cut(s) 270, 655
EcoT22I ATGCAT 1 cut(s) 1371
ErhI CCWWGG 2 cut(s) 270, 655
FalI AAGNNNNNCTT 2 cut(s) 963, 995
FauI CCCGC 1 cut(s) 755
FblI GTMKAC 1 cut(s) 879
Fnu4HI GCNGC 9 cut(s) 165, 367, 477, 633, 849, 1149, 1156, 1263, 1331
FokI GGATG 1 cut(s) 1053
Fsp4HI GCNGC 9 cut(s) 165, 367, 477, 633, 849, 1149, 1156, 1263, 1331
FspBI CTAG 2 cut(s) 384, 965
GlaI GCGC 3 cut(s) 39, 143, 939
GluI GCNGC 9 cut(s) 165, 367, 477, 633, 849, 1149, 1156, 1263, 1331
GsaI CCCAGC 1 cut(s) 1071
GsuI CTGGAG 2 cut(s) 621, 1448
HaeII RGCGCY 2 cut(s) 41, 941
HaeIII GGCC 2 cut(s) 369, 1382
HapII CCGG 5 cut(s) 35, 195, 237, 258, 1383
HgaI GACGC 2 cut(s) 926, 1091
HhaI GCGC 3 cut(s) 40, 144, 940
Hin6I GCGC 3 cut(s) 38, 142, 938
HinP1I GCGC 3 cut(s) 38, 142, 938
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 4 cut(s) 700, 869, 1014, 1243
HpaII CCGG 5 cut(s) 35, 195, 237, 258, 1383
HphI GGTGA 5 cut(s) 37, 257, 739, 1397, 1496
Hpy166II GTNNAC 2 cut(s) 302, 880
Hpy188I TCNGA 4 cut(s) 23, 100, 181, 1444
Hpy188III TCNNGA 8 cut(s) 258, 311, 560, 600, 704, 712, 886, 1249
Hpy8I GTNNAC 2 cut(s) 302, 880
Hpy99I CGWCG 2 cut(s) 20, 23
HpyAV CCTTC 7 cut(s) 20, 163, 373, 968, 1274, 1293, 1303
HpyCH4III ACNGT 2 cut(s) 109, 814
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 9 cut(s) 592, 648, 863, 895, 1099, 1148, 1155, 1365, 1369
HpyF10VI GCNNNNNNNGC 7 cut(s) 164, 629, 641, 944, 1105, 1114, 1198
HpyF3I CTNAG 3 cut(s) 480, 1045, 1180
HpySE526I ACGT 1 cut(s) 618
HspAI GCGC 3 cut(s) 38, 142, 938
Kpn2I TCCGGA 1 cut(s) 257
KroI GCCGGC 2 cut(s) 34, 194
KroNI GCCGGC 2 cut(s) 36, 196
Kzo9I GATC 4 cut(s) 438, 741, 1136, 1486
LmnI GCTCC 2 cut(s) 1283, 1429
Lsp1109I GCAGC 8 cut(s) 176, 463, 619, 835, 1160, 1167, 1249, 1342
LweI GCATC 4 cut(s) 135, 749, 1031, 1210
MaeI CTAG 2 cut(s) 384, 965
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 3 cut(s) 421, 922, 1385
MalI GATC 4 cut(s) 440, 743, 1138, 1488
MboI GATC 4 cut(s) 438, 741, 1136, 1486
MboII GAAGA 6 cut(s) 419, 971, 983, 1091, 1315, 1431
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 9 cut(s) 24, 173, 342, 415, 733, 826, 993, 1026, 1234
MlyI GAGTC 1 cut(s) 709
Mph1103I ATGCAT 1 cut(s) 1371
MreI CGCCGGCG 2 cut(s) 34, 194
MroI TCCGGA 1 cut(s) 257
MroNI GCCGGC 2 cut(s) 34, 194
MroXI GAANNNNTTC 3 cut(s) 28, 173, 978
MseI TTAA 4 cut(s) 375, 546, 639, 909
MslI CAYNNNNRTG 4 cut(s) 291, 690, 1109, 1370
MspI CCGG 5 cut(s) 35, 195, 237, 258, 1383
MspR9I CCNGG 3 cut(s) 238, 1335, 1427
MvaI CCWGG 2 cut(s) 1335, 1427
MvnI CGCG 4 cut(s) 142, 366, 947, 1419
MwoI GCNNNNNNNGC 7 cut(s) 164, 629, 641, 944, 1105, 1114, 1198
NaeI GCCGGC 2 cut(s) 36, 196
NciI CCSGG 1 cut(s) 238
NdeII GATC 4 cut(s) 438, 741, 1136, 1486
NgoMIV GCCGGC 2 cut(s) 34, 194
NlaIV GGNNCC 2 cut(s) 337, 581
NmuCI GTSAC 2 cut(s) 922, 1385
NsiI ATGCAT 1 cut(s) 1371
NspI RCATGY 4 cut(s) 355, 1108, 1369, 1373
PaeI GCATGC 2 cut(s) 1108, 1369
PdiI GCCGGC 2 cut(s) 36, 196
PdmI GAANNNNTTC 3 cut(s) 28, 173, 978
PfeI GAWTC 3 cut(s) 869, 1014, 1243
PflFI GACNNNGTC 1 cut(s) 920
PflMI CCANNNNNTGG 1 cut(s) 328
PfoI TCCNGGA 1 cut(s) 1425
PkrI GCNGC 9 cut(s) 166, 368, 478, 634, 850, 1150, 1157, 1264, 1332
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
PpuMI RGGWCCY 1 cut(s) 1423
Psp5II RGGWCCY 1 cut(s) 1423
Psp6I CCWGG 2 cut(s) 1333, 1425
PspFI CCCAGC 1 cut(s) 1067
PspGI CCWGG 2 cut(s) 1333, 1425
PspN4I GGNNCC 2 cut(s) 337, 581
PspPI GGNCC 1 cut(s) 1423
PspPPI RGGWCCY 1 cut(s) 1423
PsyI GACNNNGTC 1 cut(s) 920
RsaI GTAC 3 cut(s) 356, 449, 684
RsaNI GTAC 3 cut(s) 355, 448, 683
RseI CAYNNNNRTG 4 cut(s) 291, 690, 1109, 1370
SaqAI TTAA 4 cut(s) 375, 546, 639, 909
SatI GCNGC 9 cut(s) 165, 367, 477, 633, 849, 1149, 1156, 1263, 1331
Sau3AI GATC 4 cut(s) 438, 741, 1136, 1486
Sau96I GGNCC 1 cut(s) 1423
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 3 cut(s) 238, 1335, 1427
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 4 cut(s) 135, 749, 1031, 1210
SgrAI CRCCGGYG 2 cut(s) 34, 194
SinI GGWCC 1 cut(s) 1423
SmiMI CAYNNNNRTG 4 cut(s) 291, 690, 1109, 1370
SphI GCATGC 2 cut(s) 1108, 1369
Sse9I AATT 9 cut(s) 24, 173, 342, 415, 733, 826, 993, 1026, 1234
SsiI CCGC 6 cut(s) 158, 339, 366, 762, 945, 1417
SspMI CTAG 2 cut(s) 384, 965
StyD4I CCNGG 3 cut(s) 236, 1333, 1425
StyI CCWWGG 2 cut(s) 270, 655
TaaI ACNGT 2 cut(s) 109, 814
TaiI ACGT 1 cut(s) 621
TaqII GACCGA 1 cut(s) 1307
TasI AATT 9 cut(s) 24, 173, 342, 415, 733, 826, 993, 1026, 1234
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 3 cut(s) 869, 1014, 1243
Tru1I TTAA 4 cut(s) 375, 546, 639, 909
Tru9I TTAA 4 cut(s) 375, 546, 639, 909
TscAI CASTG 3 cut(s) 112, 744, 937
TseFI GTSAC 2 cut(s) 922, 1385
TseI GCWGC 8 cut(s) 164, 476, 632, 848, 1148, 1155, 1262, 1330
Tsp45I GTSAC 2 cut(s) 922, 1385
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 3 cut(s) 112, 744, 937
Tth111I GACNNNGTC 1 cut(s) 920
Van91I CCANNNNNTGG 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 1423
XapI RAATTY 3 cut(s) 173, 415, 826
XceI RCATGY 4 cut(s) 355, 1108, 1369, 1373
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 879
XmnI GAANNNNTTC 3 cut(s) 28, 173, 978
XspI CTAG 2 cut(s) 384, 965
Zsp2I ATGCAT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.