RchiOBHm_Chr5g0048131

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012282
Physical Location & Seq
Reverse (-)
44763797 .. 44770555
6759 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32594

Sequence Viewer

Length: 1539 bp
ATGATTCTCCCCGCCTGCACGGCAACCCCAATTCAACTTCCCAAACAACACCCACTCACCACTACTCCACACCCATCTCAGTCCACCTCTCCCAATCCAAAACATGACAAAAAACACTCGGTTTCGCTGAAAAACAGGAATGAACATATGGACCAAACTGTGTTATGGACCTCTTCTATATCCCAACGCTGCCGAAGTGGTCAGCTGTCTCAAGCTGTTTCACAGTTCATCCAAATGAGACAAGCCGGGGTCGAACCAAACCACATCACATTCGTTACACTCTTCTCCGGCTGTGCTCACTTTCCAGCTAAAGCACCATTGTTTGGGGCCTCGCTTCATGCTTATGCGTCTAAATTGGGCTTAGATAGGACTAATGTGATGGTGGGGACAGCACTAGTTGATATGTATGCAAAGTCTGGTCGGGTGGAGCTTGCTAGATTGGTTTTTGGTGGGATGGAGGTGAAGAATTCAATGTCTTGGAATACTATGATTGATGGGTATATGAGAAATGGAAATGTGAGGGATGCAGTTGAGGTGTTTGATGAAATGCCTGAGAGAGATGCTGTTTCGTGGACCGCTTTGATTGGTGGGTTTGTGAAGAGACGCCGGTTTGAGCAAGCGCTGGAGTGGTTTCGTGAGATGCAGGTGTCCGGTGTGGAACCGGACTATGTGACTATAATTGCTGTGATTGCTGCTTGTGCTGATTTGGGAACTCTTGGTTTAGGCTTCTGGATAAACCGGTTTGTTATGAAGCAAGACTTTAGACACAATATTCGGATAAGTAACTCGTTGATTGATATGTATTCCCGATGCGGATGTATTGATTTTGCTAGGCAAGTGTTTGACAACATGCCTGATCGGACCTTGGTTTCATGGAACTCTATGATTGTGGGTTTTGCCGTGAATGGGCATGCAGAGGAAGCTCTTGTGTTCTTCAACCTGATGCAGAAGGAAGGGTTCAAGCCGGACGGTGTCAGCTTCACTGGGGCTCTTACTGCATGCAGCCATGCTGGTTTAGTCGATGAGGGGCTACGTTTCTTTGATAACATGAAGAGAATACACAAAATAACCCCGAGAATTGAGCATTATGGCTGCATAGTTGATCTCTATAGCCGTGCAGGGAGGCTGGAAGATGCCTTGAATGTAATAGAGAACATGGCGATGAAGCCAAATGAAGTTGTTTTGGGTTCTTTGCTAGCTGCTTGTAGAACTAGTGGAAATATCAACTTAGCCGAAAGACTGATGAAGTACCTGTTCGAGTTGGATCCTGGTGTTGATTCGAATTATGTGCTACTTGCCAACATATATGCAGCAGATGGTAGGTGGGATGGTGCAAACAAGGTTAGAAAAACTATGAAAGACCTCGGAATCCAGAAGGTGCCAGGATTTAGTTCAGTTGAGATTGACTGTGATATTCATGAATTTGTGGCCGGTGATAAATCCCATGTTGATGCAGAGTATATATATTCAACACTAGAGCTTCTATCTTTTGAGCTCAAATTATGTGGGTATGTTCCTGAACACATTATAAGGGAATAA

Protein Analysis

512

Amino Acids

57.28

Weight (kDa)

6.47

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 53 - 100 2.4e-09 PPR repeat family
PPR_2 PF13041 156 - 186 4.6e-09 PPR repeat family
PPR_1 PF12854 156 - 183 3.7e-08 PPR repeat
PPR PF01535 159 - 186 1.4e-09 PPR repeat
PPR_2 PF13041 187 - 234 5.3e-11 PPR repeat family
PPR PF01535 189 - 219 2.2e-07 PPR repeat
PPR_2 PF13041 289 - 335 5.7e-10 PPR repeat family
PPR PF01535 290 - 320 3e-07 PPR repeat
PPR_long PF17177 305 - 409 5.4e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 407 - 467 3e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05750 AT1G05750
fragaria_vesca FvH4_3g26660
malus_domestica MD00G1049500.v1.1 MD00G1071600.v1.1 MD03G1154500.v1.1
prunus_persica Prupe.6G143700_v2.0.a1
pyrus_communis pycom03g10830 pycom11g14680
rosa_chinensis RchiOBHm_Chr5g0048131 RchiOBHm_Chr5g0048161
rosa_laevigata RLG00000034500
rosa_multiflora Rmu_sc0001408.1_g000020
rosa_roxburghii Rroxscaffold_1G00033760
rosa_rugosa Rorug05G0236900
rosa_samantha Rh5AG316000 Rh5BG326400 Rh5CG352900
rosa_wichuraiana Rw5G029620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1529
AarI CACCTGC 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 634
AccB1I GGYRCC 1 cut(s) 1378
AccB7I CCANNNNNTGG 1 cut(s) 323
AciI CCGC 3 cut(s) 12, 576, 813
AclWI GGATC 2 cut(s) 1259, 1272
AcoI YGGCCR 1 cut(s) 1428
AcsI RAATTY 2 cut(s) 466, 1421
AcyI GRCGYC 1 cut(s) 604
AfaI GTAC 1 cut(s) 1250
AfeI AGCGCT 1 cut(s) 621
AfiI CCNNNNNNNGG 2 cut(s) 323, 906
AgeI ACCGGT 1 cut(s) 738
AgsI TTSAA 6 cut(s) 35, 471, 937, 961, 1141, 1470
AhlI ACTAGT 2 cut(s) 394, 1211
AjnI CCWGG 2 cut(s) 1267, 1381
AluBI AGCT 9 cut(s) 205, 215, 308, 430, 923, 978, 1199, 1480, 1495
AluI AGCT 9 cut(s) 205, 215, 308, 430, 923, 978, 1199, 1480, 1495
Alw21I GWGCWC 2 cut(s) 298, 1497
Alw26I GTCTC 3 cut(s) 213, 232, 595
AlwI GGATC 2 cut(s) 1259, 1272
Ama87I CYCGRG 1 cut(s) 1072
Aor51HI AGCGCT 1 cut(s) 621
AoxI GGCC 2 cut(s) 327, 1428
ApeKI GCWGC 6 cut(s) 189, 692, 1002, 1092, 1199, 1310
ApoI RAATTY 2 cut(s) 466, 1421
ArsI GACNNNNNNTTYG 2 cut(s) 756, 788
AsiGI ACCGGT 1 cut(s) 738
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 5 cut(s) 151, 168, 327, 573, 861
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 49, 472, 1445
AsuII TTCGAA 1 cut(s) 1280
AsuNHI GCTAGC 1 cut(s) 1195
AvaI CYCGRG 1 cut(s) 1072
AvaII GGWCC 4 cut(s) 151, 168, 573, 861
BamHI GGATCC 1 cut(s) 1264
BanI GGYRCC 1 cut(s) 1378
BanII GRGCYC 2 cut(s) 991, 1497
Bbv12I GWGCWC 2 cut(s) 298, 1497
BbvI GCAGC 6 cut(s) 176, 679, 1014, 1079, 1186, 1322
BccI CCATC 6 cut(s) 82, 373, 448, 488, 1310, 1322
BceAI ACGGC 3 cut(s) 36, 884, 1098
BcgI CGANNNNNNTGC 2 cut(s) 1270, 1304
BciT130I CCWGG 2 cut(s) 1269, 1383
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 3 cut(s) 213, 232, 595
BcuI ACTAGT 2 cut(s) 394, 1211
BfaI CTAG 6 cut(s) 395, 435, 831, 1196, 1212, 1475
BfmI CTRYAG 1 cut(s) 1108
BfoI RGCGCY 1 cut(s) 623
BfuAI ACCTGC 1 cut(s) 634
BglI GCCNNNNNGGC 1 cut(s) 20
BisI GCNGC 6 cut(s) 190, 693, 1003, 1093, 1200, 1311
BlsI GCNGC 6 cut(s) 191, 694, 1004, 1094, 1201, 1312
Bme1390I CCNGG 3 cut(s) 247, 1269, 1383
Bme18I GGWCC 4 cut(s) 151, 168, 573, 861
BmeT110I CYCGRG 1 cut(s) 1072
BmgT120I GGNCC 5 cut(s) 151, 168, 327, 573, 861
BmiI GGNNCC 4 cut(s) 328, 660, 1266, 1380
BmrFI CCNGG 3 cut(s) 247, 1269, 1383
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 7 cut(s) 514, 550, 630, 800, 933, 1123, 1441
BmtI GCTAGC 1 cut(s) 1199
BmuI ACTGGG 1 cut(s) 993
BpmI CTGGAG 1 cut(s) 644
Bpu14I TTCGAA 1 cut(s) 1280
BpuEI CTTGAG 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 604
BsaJI CCNNGG 3 cut(s) 246, 864, 1363
BsaWI WCCGGW 3 cut(s) 650, 661, 738
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 906
Bse118I RCCGGY 3 cut(s) 606, 738, 1430
Bse1I ACTGG 1 cut(s) 988
BseBI CCWGG 2 cut(s) 1269, 1383
BseDI CCNNGG 3 cut(s) 246, 864, 1363
BseGI GGATG 5 cut(s) 228, 459, 529, 821, 1333
BseLI CCNNNNNNNGG 2 cut(s) 323, 906
BseMII CTCAG 2 cut(s) 92, 543
BseNI ACTGG 1 cut(s) 988
BseXI GCAGC 6 cut(s) 176, 679, 1014, 1079, 1186, 1322
BsgI GTGCAG 1 cut(s) 1137
BshFI GGCC 2 cut(s) 329, 1430
BshNI GGYRCC 1 cut(s) 1378
BshTI ACCGGT 1 cut(s) 738
BsiHKAI GWGCWC 2 cut(s) 298, 1497
BsiHKCI CYCGRG 1 cut(s) 1072
BsiSI CCGG 8 cut(s) 246, 288, 607, 651, 662, 739, 965, 1431
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 323, 906
BsmAI GTCTC 3 cut(s) 213, 232, 595
BsmBI CGTCTC 1 cut(s) 595
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 2 cut(s) 329, 1430
BsoBI CYCGRG 1 cut(s) 1072
Bsp119I TTCGAA 1 cut(s) 1280
Bsp1286I GDGCHC 3 cut(s) 298, 991, 1497
Bsp143I GATC 3 cut(s) 856, 1102, 1264
BspACI CCGC 3 cut(s) 12, 576, 813
BspANI GGCC 2 cut(s) 329, 1430
BspCNI CTCAG 2 cut(s) 91, 544
BspHI TCATGA 1 cut(s) 1417
BspLI GGNNCC 4 cut(s) 328, 660, 1266, 1380
BspMI ACCTGC 1 cut(s) 634
BspOI GCTAGC 1 cut(s) 1199
BspPI GGATC 2 cut(s) 1259, 1272
BspT104I TTCGAA 1 cut(s) 1280
BspT107I GGYRCC 1 cut(s) 1378
BsrFI RCCGGY 3 cut(s) 606, 738, 1430
BsrI ACTGG 1 cut(s) 988
BssAI RCCGGY 3 cut(s) 606, 738, 1430
BssECI CCNNGG 3 cut(s) 246, 864, 1363
BssMI GATC 3 cut(s) 856, 1102, 1264
BssNI GRCGYC 1 cut(s) 604
BssT1I CCWWGG 1 cut(s) 864
Bst2UI CCWGG 2 cut(s) 1269, 1383
Bst4CI ACNGT 4 cut(s) 160, 225, 971, 1409
Bst6I CTCTTC 4 cut(s) 178, 287, 593, 1046
BstACI GRCGYC 1 cut(s) 604
BstBI TTCGAA 1 cut(s) 1280
BstC8I GCNNGC 6 cut(s) 16, 432, 618, 912, 1000, 1197
BstDEI CTNAG 4 cut(s) 78, 361, 552, 1228
BstF5I GGATG 5 cut(s) 228, 459, 529, 821, 1333
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 3 cut(s) 859, 1105, 1267
BstMAI GTCTC 3 cut(s) 213, 232, 595
BstMBI GATC 3 cut(s) 856, 1102, 1264
BstMWI GCNNNNNNNGC 5 cut(s) 20, 689, 698, 920, 995
BstNI CCWGG 2 cut(s) 1269, 1383
BstNSI RCATGY 3 cut(s) 853, 914, 1002
BstSCI CCNGG 3 cut(s) 245, 1267, 1381
BstSFI CTRYAG 1 cut(s) 1108
BstV1I GCAGC 6 cut(s) 176, 679, 1014, 1079, 1186, 1322
BstX2I RGATCY 1 cut(s) 1264
BstYI RGATCY 1 cut(s) 1264
BsuRI GGCC 2 cut(s) 329, 1430
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 5 cut(s) 228, 459, 529, 821, 1333
BtsIMutI CAGTG 1 cut(s) 981
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 6 cut(s) 16, 432, 618, 912, 1000, 1197
CciI TCATGA 1 cut(s) 1417
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 3 cut(s) 606, 738, 1430
Cfr13I GGNCC 5 cut(s) 151, 168, 327, 573, 861
CseI GACGC 2 cut(s) 336, 612
Csp6I GTAC 1 cut(s) 1249
CspAI ACCGGT 1 cut(s) 738
CspCI CAANNNNNGTGG 2 cut(s) 1486, 1521
CviQI GTAC 1 cut(s) 1249
DdeI CTNAG 4 cut(s) 78, 361, 552, 1228
DpnI GATC 3 cut(s) 858, 1104, 1266
DpnII GATC 3 cut(s) 856, 1102, 1264
EaeI YGGCCR 1 cut(s) 1428
Eam1104I CTCTTC 4 cut(s) 178, 287, 593, 1046
EarI CTCTTC 4 cut(s) 178, 287, 593, 1046
Ecl136II GAGCTC 1 cut(s) 1495
Eco130I CCWWGG 1 cut(s) 864
Eco24I GRGCYC 2 cut(s) 991, 1497
Eco47I GGWCC 4 cut(s) 151, 168, 573, 861
Eco47III AGCGCT 1 cut(s) 621
Eco53kI GAGCTC 1 cut(s) 1495
Eco88I CYCGRG 1 cut(s) 1072
EcoICRI GAGCTC 1 cut(s) 1495
EcoO109I RGGNCCY 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 2 cut(s) 1267, 1381
EcoT14I CCWWGG 1 cut(s) 864
EcoT38I GRGCYC 2 cut(s) 991, 1497
ErhI CCWWGG 1 cut(s) 864
Esp3I CGTCTC 1 cut(s) 595
FalI AAGNNNNNCTT 2 cut(s) 743, 775
FaqI GGGAC 1 cut(s) 400
FauI CCCGC 1 cut(s) 19
FauNDI CATATG 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 190, 693, 1003, 1093, 1200, 1311
FokI GGATG 5 cut(s) 215, 466, 536, 828, 1340
FriOI GRGCYC 2 cut(s) 991, 1497
Fsp4HI GCNGC 6 cut(s) 190, 693, 1003, 1093, 1200, 1311
FspBI CTAG 6 cut(s) 395, 435, 831, 1196, 1212, 1475
GlaI GCGC 1 cut(s) 621
GluI GCNGC 6 cut(s) 190, 693, 1003, 1093, 1200, 1311
GsuI CTGGAG 1 cut(s) 644
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 2 cut(s) 329, 1430
HapII CCGG 8 cut(s) 246, 288, 607, 651, 662, 739, 965, 1431
HgaI GACGC 2 cut(s) 336, 612
HhaI GCGC 1 cut(s) 622
Hin1I GRCGYC 1 cut(s) 604
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HinfI GANTC 3 cut(s) 4, 1277, 1368
HpaII CCGG 8 cut(s) 246, 288, 607, 651, 662, 739, 965, 1431
HphI GGTGA 3 cut(s) 49, 472, 1445
Hpy166II GTNNAC 2 cut(s) 84, 573
Hpy188I TCNGA 3 cut(s) 777, 861, 1367
Hpy188III TCNNGA 6 cut(s) 635, 730, 807, 1372, 1418, 1517
Hpy8I GTNNAC 2 cut(s) 84, 573
HpyAV CCTTC 3 cut(s) 943, 947, 1369
HpyCH4III ACNGT 4 cut(s) 160, 225, 971, 1409
HpyCH4IV ACGT 1 cut(s) 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 689, 698, 920, 995
HpyF3I CTNAG 4 cut(s) 78, 361, 552, 1228
HpySE526I ACGT 1 cut(s) 1033
Hsp92I GRCGYC 1 cut(s) 604
HspAI GCGC 1 cut(s) 620
Kzo9I GATC 3 cut(s) 856, 1102, 1264
LmnI GCTCC 1 cut(s) 427
Lsp1109I GCAGC 6 cut(s) 176, 679, 1014, 1079, 1186, 1322
LweI GCATC 7 cut(s) 514, 550, 630, 800, 933, 1123, 1441
MaeI CTAG 6 cut(s) 395, 435, 831, 1196, 1212, 1475
MaeII ACGT 1 cut(s) 1033
MaeIII GTNAC 3 cut(s) 274, 670, 782
MalI GATC 3 cut(s) 858, 1104, 1266
MboI GATC 3 cut(s) 856, 1102, 1264
MboII GAAGA 7 cut(s) 165, 274, 475, 610, 925, 1063, 1142
MflI RGATCY 1 cut(s) 1264
MhlI GDGCHC 3 cut(s) 298, 991, 1497
MluCI AATT 8 cut(s) 30, 353, 466, 678, 1077, 1282, 1421, 1499
MmeI TCCRAC 1 cut(s) 1242
MslI CAYNNNNRTG 4 cut(s) 233, 342, 1160, 1449
MspA1I CMGCKG 1 cut(s) 205
MspI CCGG 8 cut(s) 246, 288, 607, 651, 662, 739, 965, 1431
MspR9I CCNGG 3 cut(s) 247, 1269, 1383
MvaI CCWGG 2 cut(s) 1269, 1383
MwoI GCNNNNNNNGC 5 cut(s) 20, 689, 698, 920, 995
NciI CCSGG 1 cut(s) 247
NdeI CATATG 1 cut(s) 147
NdeII GATC 3 cut(s) 856, 1102, 1264
NheI GCTAGC 1 cut(s) 1195
NlaIV GGNNCC 4 cut(s) 328, 660, 1266, 1380
NmuCI GTSAC 1 cut(s) 670
NspI RCATGY 3 cut(s) 853, 914, 1002
NspV TTCGAA 1 cut(s) 1280
PaeI GCATGC 2 cut(s) 914, 1002
PagI TCATGA 1 cut(s) 1417
PaqCI CACCTGC 1 cut(s) 634
PfeI GAWTC 3 cut(s) 4, 1277, 1368
PflFI GACNNNGTC 1 cut(s) 971
PflMI CCANNNNNTGG 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 738
PkrI GCNGC 6 cut(s) 191, 694, 1004, 1094, 1201, 1312
PsiI TTATAA 1 cut(s) 1529
Psp124BI GAGCTC 1 cut(s) 1497
Psp6I CCWGG 2 cut(s) 1267, 1381
PspGI CCWGG 2 cut(s) 1267, 1381
PspN4I GGNNCC 4 cut(s) 328, 660, 1266, 1380
PspPI GGNCC 5 cut(s) 151, 168, 327, 573, 861
PsuI RGATCY 1 cut(s) 1264
PsyI GACNNNGTC 1 cut(s) 971
PvuII CAGCTG 1 cut(s) 205
RsaI GTAC 1 cut(s) 1250
RsaNI GTAC 1 cut(s) 1249
RseI CAYNNNNRTG 4 cut(s) 233, 342, 1160, 1449
SacI GAGCTC 1 cut(s) 1497
SatI GCNGC 6 cut(s) 190, 693, 1003, 1093, 1200, 1311
Sau3AI GATC 3 cut(s) 856, 1102, 1264
Sau96I GGNCC 5 cut(s) 151, 168, 327, 573, 861
ScrFI CCNGG 3 cut(s) 247, 1269, 1383
SduI GDGCHC 3 cut(s) 298, 991, 1497
SfaNI GCATC 7 cut(s) 514, 550, 630, 800, 933, 1123, 1441
SfcI CTRYAG 1 cut(s) 1108
SfuI TTCGAA 1 cut(s) 1280
SinI GGWCC 4 cut(s) 151, 168, 573, 861
SmiMI CAYNNNNRTG 4 cut(s) 233, 342, 1160, 1449
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SpeI ACTAGT 2 cut(s) 394, 1211
SphI GCATGC 2 cut(s) 914, 1002
Sse9I AATT 8 cut(s) 30, 353, 466, 678, 1077, 1282, 1421, 1499
SsiI CCGC 3 cut(s) 12, 576, 813
SspI AATATT 1 cut(s) 772
SspMI CTAG 6 cut(s) 395, 435, 831, 1196, 1212, 1475
SstI GAGCTC 1 cut(s) 1497
StyD4I CCNGG 3 cut(s) 245, 1267, 1381
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 4 cut(s) 160, 225, 971, 1409
TaiI ACGT 1 cut(s) 1036
TaqI TCGA 4 cut(s) 252, 1020, 1257, 1280
TasI AATT 8 cut(s) 30, 353, 466, 678, 1077, 1282, 1421, 1499
TfiI GAWTC 3 cut(s) 4, 1277, 1368
TscAI CASTG 1 cut(s) 988
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 6 cut(s) 189, 692, 1002, 1092, 1199, 1310
Tsp45I GTSAC 1 cut(s) 670
TspRI CASTG 1 cut(s) 988
Tth111I GACNNNGTC 1 cut(s) 971
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 4 cut(s) 151, 168, 573, 861
XapI RAATTY 2 cut(s) 466, 1421
XceI RCATGY 3 cut(s) 853, 914, 1002
XspI CTAG 6 cut(s) 395, 435, 831, 1196, 1212, 1475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.