AT1G08340

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010796
Physical Location & Seq
Forward (+)
2630891 .. 2632871
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G08340.1

Sequence Viewer

Length: 996 bp
ATGGACATTGGCGGTCCAACCAATATCCGCCACGTGGCTCACGTTACCTTCGATCGCTTCGATGGCTTCCTCGGCCTTCCCTCTGAGTTCGAGCCTGATGTCCCCAGAAAAGCTCCCAGTGCAAGTGCAACGGTGTTTGGGGTATCGACAGAGTCAATGCAGTTATCATACGACTCGAGAGGGAACTGTGTTCCTGTGATACTGTTGCTATTGCAGAGCCGGCTTTATGATCAAGGAGGCTTGCAGGCAGAAGGAGTATTCAGAATCACTGGAGAGAACAGCGAGGAAGAGTTTGTTAGGGAGCAGCTTAATAAAGGGATCATACCTGATGGGATTGATGTCCATTGTTTAGCCGGTCTTATTAAGGCTTGGTTCAGAGAGCTGCCGAGAGGGGTACTGGATCCTCTGCCATCGGAGCAAGTGATGCAGTGTGAGTCAGATGAGGATTTTGTCAAAGTTGTGAGACTTTTACCTCAAACAGAAGCTTCTCTTCTCAATTGGGCCATCAATCTCATGGCTGATGTTATTCAGTTTGAGCATGTTAATAAGATGAACTCTCGTAACCTTGCTTTAGTCTTTGCCCCCAACATGTCTCAGATGGCAGACCCTTTAACTGCATTGATGTATGCGGTCCAAGTGATGAAGTTGCTCAAGAGCCTCACAGAGAAGACTGTGAGAGAAAGGGAAGCTTCCTCTTCTGTGGTTGACAGAAGATGTAGCAAAGAAGCCGAAGATGGCGAGAAAGAAAAAGACAATGAAGAGGAAGAAGAAGATGAAGAAGAGGAGGAAGAAGAAGAAGATGAAGATGAAGATGAAGAAGAAGAAGGAGATGGTGTGTACATAATTAAGGAGGAAGAAGCATCAGAGATAATAAAAGTGGTTGCTGATGAACACAAGTCAGGAAGCATAAAGAGTGAGTTTGAGGGATCTAGTGCTACGGATTCAAAGGGAGATAATGGAGTTGTGCAGCCACCCATTTGCAGCTCGAACCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0034059 GO:0036293 GO:0044464 GO:0050896 GO:0070482 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

331

Amino Acids

36.83

Weight (kDa)

4.32

Isoelectric Point (pI)

60.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 71 - 199 9.8e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 12, 28, 629
AclWI GGATC 4 cut(s) 326, 395, 408, 934
AcvI CACGTG 1 cut(s) 34
AfaI GTAC 2 cut(s) 396, 839
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 1 cut(s) 945
AluBI AGCT 6 cut(s) 113, 307, 382, 485, 689, 984
AluI AGCT 6 cut(s) 113, 307, 382, 485, 689, 984
Alw26I GTCTC 2 cut(s) 457, 597
AlwI GGATC 4 cut(s) 326, 395, 408, 934
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 2 cut(s) 73, 501
ApeKI GCWGC 4 cut(s) 304, 382, 967, 981
AspS9I GGNCC 3 cut(s) 14, 501, 631
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 2 cut(s) 14, 631
BamHI GGATCC 1 cut(s) 400
BbrPI CACGTG 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 674
BbvI GCAGC 3 cut(s) 316, 369, 979
BccI CCATC 7 cut(s) 56, 323, 418, 512, 592, 728, 824
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 457, 597
BfaI CTAG 1 cut(s) 930
BisI GCNGC 4 cut(s) 305, 383, 968, 982
BlsI GCNGC 4 cut(s) 306, 384, 969, 983
Bme18I GGWCC 2 cut(s) 14, 631
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 3 cut(s) 14, 501, 631
BmiI GGNNCC 1 cut(s) 402
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 414, 869
BmuI ACTGGG 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 674
BpmI CTGGAG 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 2 cut(s) 219, 353
Bse1I ACTGG 3 cut(s) 117, 274, 402
BseDI CCNNGG 1 cut(s) 70
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 75, 608
BseNI ACTGG 3 cut(s) 117, 274, 402
BseRI GAGGAG 1 cut(s) 797
BseXI GCAGC 3 cut(s) 316, 369, 979
BsgI GTGCAG 1 cut(s) 986
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 75, 503
BsiEI CGRYCG 1 cut(s) 55
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 2 cut(s) 220, 354
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 457, 597
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 2 cut(s) 75, 503
BsoBI CYCGRG 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 837
Bsp143I GATC 5 cut(s) 52, 229, 318, 400, 926
BspACI CCGC 3 cut(s) 12, 28, 629
BspANI GGCC 2 cut(s) 75, 503
BspCNI CTCAG 2 cut(s) 76, 607
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 4 cut(s) 326, 395, 408, 934
BsrFI RCCGGY 2 cut(s) 219, 353
BsrGI TGTACA 1 cut(s) 837
BsrI ACTGG 3 cut(s) 117, 274, 402
BssAI RCCGGY 2 cut(s) 219, 353
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 5 cut(s) 52, 229, 318, 400, 926
Bst4CI ACNGT 4 cut(s) 133, 188, 204, 673
Bst6I CTCTTC 5 cut(s) 282, 495, 700, 753, 774
BstAPI GCANNNNNTGC 1 cut(s) 424
BstAUI TGTACA 1 cut(s) 837
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 3 cut(s) 221, 242, 246
BstDEI CTNAG 2 cut(s) 84, 594
BstKTI GATC 5 cut(s) 55, 232, 321, 403, 929
BstMAI GTCTC 2 cut(s) 457, 597
BstMBI GATC 5 cut(s) 52, 229, 318, 400, 926
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 6 cut(s) 63, 72, 119, 220, 415, 424
BstNSI RCATGY 2 cut(s) 542, 592
BstV1I GCAGC 3 cut(s) 316, 369, 979
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 2 cut(s) 400, 926
BstYI RGATCY 2 cut(s) 400, 926
BsuRI GGCC 2 cut(s) 75, 503
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 3 cut(s) 124, 267, 434
Cac8I GCNNGC 3 cut(s) 221, 242, 246
Cfr10I RCCGGY 2 cut(s) 219, 353
Cfr13I GGNCC 3 cut(s) 14, 501, 631
Csp6I GTAC 2 cut(s) 395, 838
CviAII CATG 3 cut(s) 514, 539, 589
CviQI GTAC 2 cut(s) 395, 838
DdeI CTNAG 2 cut(s) 84, 594
DpnI GATC 5 cut(s) 54, 231, 320, 402, 928
DpnII GATC 5 cut(s) 52, 229, 318, 400, 926
Eam1104I CTCTTC 5 cut(s) 282, 495, 700, 753, 774
EarI CTCTTC 5 cut(s) 282, 495, 700, 753, 774
EciI GGCGGA 1 cut(s) 17
Eco47I GGWCC 2 cut(s) 14, 631
Eco72I CACGTG 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 175
FaeI CATG 3 cut(s) 517, 542, 592
FaiI YATR 9 cut(s) 169, 228, 323, 515, 540, 590, 627, 842, 908
FalI AAGNNNNNCTT 4 cut(s) 474, 506, 673, 705
FaqI GGGAC 1 cut(s) 86
FatI CATG 3 cut(s) 513, 538, 588
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 4 cut(s) 305, 383, 968, 982
Fsp4HI GCNGC 4 cut(s) 305, 383, 968, 982
FspBI CTAG 1 cut(s) 930
GluI GCNGC 4 cut(s) 305, 383, 968, 982
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 75, 503
HapII CCGG 2 cut(s) 220, 354
Hin1II CATG 3 cut(s) 517, 542, 592
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 2 cut(s) 483, 687
HinfI GANTC 5 cut(s) 152, 173, 264, 434, 941
HpaII CCGG 2 cut(s) 220, 354
Hpy166II GTNNAC 2 cut(s) 706, 838
Hpy188I TCNGA 7 cut(s) 85, 263, 377, 415, 439, 597, 865
Hpy188III TCNNGA 3 cut(s) 177, 652, 900
Hpy8I GTNNAC 2 cut(s) 706, 838
HpyAV CCTTC 4 cut(s) 58, 86, 245, 818
HpyCH4III ACNGT 4 cut(s) 133, 188, 204, 673
HpyCH4IV ACGT 2 cut(s) 33, 42
HpyCH4V TGCA 9 cut(s) 122, 128, 160, 214, 244, 427, 617, 967, 981
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 72, 119, 220, 415, 424
HpyF3I CTNAG 2 cut(s) 84, 594
HpySE526I ACGT 2 cut(s) 33, 42
Hsp92II CATG 3 cut(s) 517, 542, 592
KroI GCCGGC 1 cut(s) 219
KroNI GCCGGC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 5 cut(s) 52, 229, 318, 400, 926
LmnI GCTCC 3 cut(s) 118, 301, 415
Lsp1109I GCAGC 3 cut(s) 316, 369, 979
LweI GCATC 2 cut(s) 414, 869
MaeI CTAG 1 cut(s) 930
MaeII ACGT 2 cut(s) 33, 42
MaeIII GTNAC 2 cut(s) 43, 560
MalI GATC 5 cut(s) 54, 231, 320, 402, 928
MboI GATC 5 cut(s) 52, 229, 318, 400, 926
MfeI CAATTG 1 cut(s) 496
MflI RGATCY 2 cut(s) 400, 926
MluCI AATT 2 cut(s) 496, 843
MlyI GAGTC 3 cut(s) 161, 167, 443
MmeI TCCRAC 1 cut(s) 41
MroNI GCCGGC 1 cut(s) 219
MseI TTAA 5 cut(s) 309, 363, 543, 611, 846
MspI CCGG 2 cut(s) 220, 354
MunI CAATTG 1 cut(s) 496
MwoI GCNNNNNNNGC 6 cut(s) 63, 72, 119, 220, 415, 424
NaeI GCCGGC 1 cut(s) 221
NdeII GATC 5 cut(s) 52, 229, 318, 400, 926
NgoMIV GCCGGC 1 cut(s) 219
NlaIII CATG 3 cut(s) 517, 542, 592
NlaIV GGNNCC 1 cut(s) 402
NmeAIII GCCGAG 2 cut(s) 51, 411
NspI RCATGY 2 cut(s) 542, 592
PaeR7I CTCGAG 1 cut(s) 175
PciI ACATGT 1 cut(s) 588
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 48, 57
PdiI GCCGGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 264, 941
PflFI GACNNNGTC 1 cut(s) 151
PkrI GCNGC 4 cut(s) 306, 384, 969, 983
Ple19I CGATCG 1 cut(s) 55
PleI GAGTC 3 cut(s) 160, 167, 442
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 3 cut(s) 160, 167, 442
Ppu21I YACGTR 1 cut(s) 34
PscI ACATGT 1 cut(s) 588
PspCI CACGTG 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 3 cut(s) 14, 501, 631
PsuI RGATCY 2 cut(s) 400, 926
PsyI GACNNNGTC 1 cut(s) 151
PvuI CGATCG 1 cut(s) 55
RsaI GTAC 2 cut(s) 396, 839
RsaNI GTAC 2 cut(s) 395, 838
SaqAI TTAA 5 cut(s) 309, 363, 543, 611, 846
SatI GCNGC 4 cut(s) 305, 383, 968, 982
Sau3AI GATC 5 cut(s) 52, 229, 318, 400, 926
Sau96I GGNCC 3 cut(s) 14, 501, 631
SchI GAGTC 3 cut(s) 161, 167, 443
SfaNI GCATC 2 cut(s) 414, 869
Sfr274I CTCGAG 1 cut(s) 175
SinI GGWCC 2 cut(s) 14, 631
SlaI CTCGAG 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 175, 650
SmoI CTYRAG 2 cut(s) 175, 650
Sse9I AATT 2 cut(s) 496, 843
SsiI CCGC 3 cut(s) 12, 28, 629
SspMI CTAG 1 cut(s) 930
TaaI ACNGT 4 cut(s) 133, 188, 204, 673
TaiI ACGT 2 cut(s) 36, 45
TaqI TCGA 6 cut(s) 51, 60, 90, 146, 176, 986
TasI AATT 2 cut(s) 496, 843
TatI WGTACW 1 cut(s) 837
TfiI GAWTC 2 cut(s) 264, 941
Tru1I TTAA 5 cut(s) 309, 363, 543, 611, 846
Tru9I TTAA 5 cut(s) 309, 363, 543, 611, 846
TscAI CASTG 3 cut(s) 124, 274, 434
TseI GCWGC 4 cut(s) 304, 382, 967, 981
TspDTI ATGAA 8 cut(s) 566, 656, 771, 789, 816, 822, 828, 903
TspGWI ACGGA 1 cut(s) 953
TspRI CASTG 3 cut(s) 124, 274, 434
Tth111I GACNNNGTC 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 14, 631
XceI RCATGY 2 cut(s) 542, 592
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 175
XspI CTAG 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.