AT5G22400

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010796
Physical Location & Seq
Reverse (-)
7422670 .. 7425574
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22400.1

Sequence Viewer

Length: 1401 bp
ATGACTGAAGTTCTTCACTTTCCTTCATCTCCAAGCGCTTCTCATTCATCTTCTTCTTCTTCTTCTTCTCCTTCACCTTCTTCTTTATCTTACGCCTCTCGCTCTAATGCGACTCTCTTGATTAGCTCTGACCACAACCGGAGAAACCCAGTTGCTAGATTCGATCAAGATGTTGACTTTCATGCCTCAATCGAAGAACAAGATTTGAGAAGACGGAGCAGTACCGATGGAGGAGAAGAAGACGATGGTGGGGAAGATCAGATTTCGTTGTTGGCTCTTCTTGTTGCCATTTTCAGGAGATCTTTGATTTCTTGCAAGAGTAACCGGAGGGAGCTTTGTAGCATGGAGATTGGATGGCCTACCAATGTCAGACACGTGGCGCACGTTACCTTTGATCGTTTCAATGGCTTCTTGGGTTTGCCTGTTGAATTCGAGCCTGAAGTTCCTAGAAGAGCTCCAAGCGCCAGTGCAACAGTCTTTGGGGTATCAACCGAATCAATGCAATTATCGTATGATTCAAGAGGCAATTGTGTACCAACCATACTATTGCTGATGCAAAACTGTTTATATAGTCAAGGAGGCTTGCAGGCAGAGGGCATTTTTAGACTCACTGCTGAGAATAGTGAGGAAGAGGCGGTTAGGGAACAATTAAACCGAGGATTTATACCTGAGCGAATCGATGTTCACTGTTTGGCAGGGCTTATCAAGGCATGGTTTAGAGAACTGCCGACAAGCGTTCTTGATTCGTTGTCGCCTGAACAGGTGATGCAGTGCCAAACAGAAGAGGAAAATGTTGAGCTCGTTAGGCTTCTTCCACCTACAGAAGCTGCTCTACTTGATTGGGCCATCAATCTAATGGCAGATGTTGTTCAGTATGAACATCTAAACAAGATGAATTCACGCAACATCGCTATGGTTTTCGCACCAAATATGACACAGATGGATGATCCACTGACAGCACTGATGTATGCGGTTCAAGTGATGAACTTTCTCAAGACACTAATCGAAAAAACTTTAAGAGAAAGGCAAGACTCAGTGGTCGAGCAAGCTCATGCATTCCCTTTAGAACCGTCTGATGAGAGTGGTCACCAAAGCCCTTCACAATCTTTGGCTTTTAACACCAGTGAGCAGAGTGAAGAGACGCAATCAGACAACATCGAAAATGCTGAAAATCAGAGTTCAAGCAGTGAGATATCAGACGAATTAACCCTAGAGAACAATGCATGTGAACAGAGAGAAACAGACTTTGGAAAATACAGAACAGGAAGATTGAGCGACTCGAGTCAACAGGTGGTGCTGAATCTAGATCCTCCAGCTCAGTGGCCAGTGGGCAGAACAAAGGGGTTGACCAACTTGAGCCGTGTAGGATCGAGGGTAGAGCGTACTGAAGCTTGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0034059 GO:0036293 GO:0044464 GO:0050896 GO:0070482 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

466

Amino Acids

51.99

Weight (kDa)

4.76

Isoelectric Point (pI)

74.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 179 - 313 1.5e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
AciI CCGC 3 cut(s) 635, 971, 1396
AclWI GGATC 3 cut(s) 941, 1301, 1375
AcoI YGGCCR 1 cut(s) 1322
AcsI RAATTY 2 cut(s) 428, 895
AcuI CTGAAG 2 cut(s) 27, 459
AcvI CACGTG 1 cut(s) 376
AfaI GTAC 3 cut(s) 223, 534, 1384
AfeI AGCGCT 1 cut(s) 37
AfiI CCNNNNNNNGG 1 cut(s) 294
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 5 cut(s) 403, 428, 519, 977, 1182
AjuI GAANNNNNNNTTGG 2 cut(s) 1230, 1262
AluBI AGCT 8 cut(s) 126, 334, 455, 799, 827, 1049, 1316, 1391
AluI AGCT 8 cut(s) 126, 334, 455, 799, 827, 1049, 1316, 1391
Alw21I GWGCWC 2 cut(s) 457, 801
Alw26I GTCTC 1 cut(s) 1133
AlwI GGATC 3 cut(s) 941, 1301, 1375
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 1 cut(s) 1279
Aor51HI AGCGCT 1 cut(s) 37
AoxI GGCC 3 cut(s) 356, 843, 1322
ApeKI GCWGC 1 cut(s) 827
ApoI RAATTY 2 cut(s) 428, 895
Asp700I GAANNNNTTC 1 cut(s) 12
AspLEI GCGC 3 cut(s) 38, 382, 464
AspS9I GGNCC 1 cut(s) 843
AsuHPI GGTGA 3 cut(s) 66, 775, 1079
AvaI CYCGRG 1 cut(s) 1279
BalI TGGCCA 1 cut(s) 1324
BanII GRGCYC 2 cut(s) 457, 801
BarI GAAGNNNNNNTAC 2 cut(s) 816, 848
BbrPI CACGTG 1 cut(s) 376
BbsI GAAGAC 2 cut(s) 217, 246
Bbv12I GWGCWC 2 cut(s) 457, 801
BbvI GCAGC 1 cut(s) 814
BccI CCATC 5 cut(s) 221, 239, 348, 854, 934
BceAI ACGGC 1 cut(s) 1344
BcoDI GTCTC 1 cut(s) 1133
BfaI CTAG 4 cut(s) 156, 447, 1211, 1304
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 2 cut(s) 39, 465
BglII AGATCT 1 cut(s) 299
BisI GCNGC 1 cut(s) 828
BlsI GCNGC 1 cut(s) 829
BmeT110I CYCGRG 1 cut(s) 1279
BmgT120I GGNCC 1 cut(s) 843
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 543, 756
BmuI ACTGGG 1 cut(s) 143
BoxI GACNNNNGTC 1 cut(s) 1281
BpiI GAAGAC 2 cut(s) 217, 246
BpmI CTGGAG 1 cut(s) 1296
Bpu10I CCTNAGC 1 cut(s) 669
BpuEI CTTGAG 2 cut(s) 977, 1375
Bsa29I ATCGAT 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 376
BsaJI CCNNGG 1 cut(s) 655
BsaWI WCCGGW 2 cut(s) 138, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 4 cut(s) 149, 465, 1122, 1325
BseCI ATCGAT 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 2 cut(s) 359, 949
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 4 cut(s) 606, 660, 1047, 1331
BseNI ACTGG 4 cut(s) 149, 465, 1122, 1325
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 1 cut(s) 814
BshFI GGCC 3 cut(s) 358, 845, 1324
BshVI ATCGAT 1 cut(s) 678
BsiHKAI GWGCWC 2 cut(s) 457, 801
BsiHKCI CYCGRG 1 cut(s) 1279
BsiSI CCGG 2 cut(s) 139, 325
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 1133
BsmBI CGTCTC 1 cut(s) 1133
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 3 cut(s) 358, 845, 1324
BsoBI CYCGRG 1 cut(s) 1279
Bsp1286I GDGCHC 2 cut(s) 457, 801
Bsp143I GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
BspACI CCGC 3 cut(s) 635, 971, 1396
BspANI GGCC 3 cut(s) 358, 845, 1324
BspCNI CTCAG 4 cut(s) 607, 661, 1046, 1330
BspDI ATCGAT 1 cut(s) 678
BspPI GGATC 3 cut(s) 941, 1301, 1375
BspQI GCTCTTC 2 cut(s) 282, 445
BsrI ACTGG 4 cut(s) 149, 465, 1122, 1325
BssECI CCNNGG 1 cut(s) 655
BssMI GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
Bst4CI ACNGT 4 cut(s) 475, 563, 689, 1071
Bst6I CTCTTC 5 cut(s) 282, 445, 624, 777, 1131
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 584, 588, 1047
BstDEI CTNAG 4 cut(s) 615, 669, 1033, 1317
BstEII GGTNACC 1 cut(s) 1085
BstF5I GGATG 2 cut(s) 359, 949
BstH2I RGCGCY 2 cut(s) 39, 465
BstHHI GCGC 3 cut(s) 38, 382, 464
BstKTI GATC 7 cut(s) 166, 259, 302, 397, 949, 1309, 1370
BstMAI GTCTC 1 cut(s) 1133
BstMBI GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
BstMWI GCNNNNNNNGC 2 cut(s) 461, 805
BstNSI RCATGY 1 cut(s) 1227
BstPAI GACNNNNGTC 1 cut(s) 1281
BstPI GGTNACC 1 cut(s) 1085
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 1 cut(s) 814
BstV2I GAAGAC 2 cut(s) 217, 246
BstX2I RGATCY 2 cut(s) 299, 1306
BstXI CCANNNNNNTGG 1 cut(s) 1320
BstYI RGATCY 2 cut(s) 299, 1306
Bsu15I ATCGAT 1 cut(s) 678
BsuRI GGCC 3 cut(s) 358, 845, 1324
BsuTUI ATCGAT 1 cut(s) 678
BtgZI GCGATG 1 cut(s) 892
BtsCI GGATG 2 cut(s) 359, 949
BtsI GCAGTG 3 cut(s) 609, 776, 1192
Cac8I GCNNGC 3 cut(s) 584, 588, 1047
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 3 cut(s) 38, 382, 464
Cfr13I GGNCC 1 cut(s) 843
ClaI ATCGAT 1 cut(s) 678
CseI GACGC 1 cut(s) 1150
Csp6I GTAC 3 cut(s) 222, 533, 1383
CviAII CATG 5 cut(s) 182, 343, 711, 1052, 1224
CviQI GTAC 3 cut(s) 222, 533, 1383
DdeI CTNAG 4 cut(s) 615, 669, 1033, 1317
DpnI GATC 7 cut(s) 165, 258, 301, 396, 948, 1308, 1369
DpnII GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
EaeI YGGCCR 1 cut(s) 1322
Eam1104I CTCTTC 5 cut(s) 282, 445, 624, 777, 1131
EarI CTCTTC 5 cut(s) 282, 445, 624, 777, 1131
Ecl136II GAGCTC 2 cut(s) 455, 799
Eco24I GRGCYC 2 cut(s) 457, 801
Eco32I GATATC 1 cut(s) 1194
Eco47III AGCGCT 1 cut(s) 37
Eco53kI GAGCTC 2 cut(s) 455, 799
Eco57I CTGAAG 2 cut(s) 27, 459
Eco72I CACGTG 1 cut(s) 376
Eco88I CYCGRG 1 cut(s) 1279
Eco91I GGTNACC 1 cut(s) 1085
EcoICRI GAGCTC 2 cut(s) 455, 799
EcoO65I GGTNACC 1 cut(s) 1085
EcoRI GAATTC 2 cut(s) 428, 895
EcoRV GATATC 1 cut(s) 1194
EcoT22I ATGCAT 2 cut(s) 1057, 1225
EcoT38I GRGCYC 2 cut(s) 457, 801
Esp3I CGTCTC 1 cut(s) 1133
FaeI CATG 5 cut(s) 185, 346, 714, 1055, 1227
FatI CATG 5 cut(s) 181, 342, 710, 1051, 1223
Fnu4HI GCNGC 1 cut(s) 828
FokI GGATG 2 cut(s) 366, 956
FriOI GRGCYC 2 cut(s) 457, 801
Fsp4HI GCNGC 1 cut(s) 828
FspBI CTAG 4 cut(s) 156, 447, 1211, 1304
GlaI GCGC 3 cut(s) 37, 381, 463
GluI GCNGC 1 cut(s) 828
GsuI CTGGAG 1 cut(s) 1296
HaeII RGCGCY 2 cut(s) 39, 465
HaeIII GGCC 3 cut(s) 358, 845, 1324
HapII CCGG 2 cut(s) 139, 325
HgaI GACGC 1 cut(s) 1150
HhaI GCGC 3 cut(s) 38, 382, 464
Hin1II CATG 5 cut(s) 185, 346, 714, 1055, 1227
Hin6I GCGC 3 cut(s) 36, 380, 462
HinP1I GCGC 3 cut(s) 36, 380, 462
HincII GTYRAC 3 cut(s) 175, 1286, 1347
HindII GTYRAC 3 cut(s) 175, 1286, 1347
HindIII AAGCTT 1 cut(s) 1389
HpaII CCGG 2 cut(s) 139, 325
HphI GGTGA 3 cut(s) 66, 775, 1079
Hpy166II GTNNAC 6 cut(s) 175, 533, 685, 1229, 1286, 1347
Hpy188I TCNGA 7 cut(s) 130, 261, 371, 1075, 1150, 1176, 1198
Hpy188III TCNNGA 7 cut(s) 118, 167, 295, 519, 740, 994, 1304
Hpy8I GTNNAC 6 cut(s) 175, 533, 685, 1229, 1286, 1347
HpyAV CCTTC 4 cut(s) 33, 81, 87, 1107
HpyCH4III ACNGT 4 cut(s) 475, 563, 689, 1071
HpyCH4IV ACGT 2 cut(s) 375, 384
HpyCH4V TGCA 8 cut(s) 315, 470, 502, 556, 586, 769, 1055, 1223
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 805
HpyF3I CTNAG 4 cut(s) 615, 669, 1033, 1317
HpySE526I ACGT 2 cut(s) 375, 384
Hsp92II CATG 5 cut(s) 185, 346, 714, 1055, 1227
HspAI GCGC 3 cut(s) 36, 380, 462
Kzo9I GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
LguI GCTCTTC 2 cut(s) 282, 445
LmnI GCTCC 3 cut(s) 216, 331, 460
Lsp1109I GCAGC 1 cut(s) 814
LweI GCATC 2 cut(s) 543, 756
MaeI CTAG 4 cut(s) 156, 447, 1211, 1304
MaeII ACGT 2 cut(s) 375, 384
MaeIII GTNAC 3 cut(s) 320, 385, 1085
MalI GATC 7 cut(s) 165, 258, 301, 396, 948, 1308, 1369
MboI GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
MfeI CAATTG 1 cut(s) 526
MflI RGATCY 2 cut(s) 299, 1306
MhlI GDGCHC 2 cut(s) 457, 801
MlsI TGGCCA 1 cut(s) 1324
MluCI AATT 6 cut(s) 428, 503, 526, 647, 895, 1202
MluNI TGGCCA 1 cut(s) 1324
MlyI GAGTC 5 cut(s) 106, 600, 1025, 1271, 1291
Mox20I TGGCCA 1 cut(s) 1324
Mph1103I ATGCAT 2 cut(s) 1057, 1225
MroXI GAANNNNTTC 1 cut(s) 12
MscI TGGCCA 1 cut(s) 1324
MseI TTAA 4 cut(s) 650, 1016, 1116, 1205
MslI CAYNNNNRTG 1 cut(s) 911
Msp20I TGGCCA 1 cut(s) 1324
MspI CCGG 2 cut(s) 139, 325
MunI CAATTG 1 cut(s) 526
Mva1269I GAATGC 1 cut(s) 1055
MwoI GCNNNNNNNGC 2 cut(s) 461, 805
NdeII GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
NlaIII CATG 5 cut(s) 185, 346, 714, 1055, 1227
NmuCI GTSAC 1 cut(s) 1085
NsiI ATGCAT 2 cut(s) 1057, 1225
NspI RCATGY 1 cut(s) 1227
PaeR7I CTCGAG 1 cut(s) 1279
PciSI GCTCTTC 2 cut(s) 282, 445
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PctI GAATGC 1 cut(s) 1055
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 6 cut(s) 159, 494, 515, 675, 743, 1300
PkrI GCNGC 1 cut(s) 829
PleI GAGTC 5 cut(s) 106, 600, 1025, 1271, 1290
PmaCI CACGTG 1 cut(s) 376
PmlI CACGTG 1 cut(s) 376
PpsI GAGTC 5 cut(s) 106, 600, 1025, 1271, 1290
Ppu21I YACGTR 1 cut(s) 376
PshAI GACNNNNGTC 1 cut(s) 1281
Psp124BI GAGCTC 2 cut(s) 457, 801
PspCI CACGTG 1 cut(s) 376
PspEI GGTNACC 1 cut(s) 1085
PspPI GGNCC 1 cut(s) 843
PspXI VCTCGAGB 1 cut(s) 1279
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 299, 1306
RsaI GTAC 3 cut(s) 223, 534, 1384
RsaNI GTAC 3 cut(s) 222, 533, 1383
RseI CAYNNNNRTG 1 cut(s) 911
SacI GAGCTC 2 cut(s) 457, 801
SapI GCTCTTC 2 cut(s) 282, 445
SaqAI TTAA 4 cut(s) 650, 1016, 1116, 1205
SatI GCNGC 1 cut(s) 828
Sau3AI GATC 7 cut(s) 163, 256, 299, 394, 946, 1306, 1367
Sau96I GGNCC 1 cut(s) 843
SchI GAGTC 5 cut(s) 106, 600, 1025, 1271, 1291
SduI GDGCHC 2 cut(s) 457, 801
SfaNI GCATC 2 cut(s) 543, 756
SfcI CTRYAG 1 cut(s) 819
Sfr274I CTCGAG 1 cut(s) 1279
SlaI CTCGAG 1 cut(s) 1279
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 3 cut(s) 992, 1279, 1354
SmoI CTYRAG 3 cut(s) 992, 1279, 1354
Sse9I AATT 6 cut(s) 428, 503, 526, 647, 895, 1202
SsiI CCGC 3 cut(s) 635, 971, 1396
SspMI CTAG 4 cut(s) 156, 447, 1211, 1304
SstI GAGCTC 2 cut(s) 457, 801
TaaI ACNGT 4 cut(s) 475, 563, 689, 1071
TaiI ACGT 2 cut(s) 378, 387
TaqI TCGA 9 cut(s) 162, 192, 432, 678, 1005, 1041, 1158, 1280, 1370
TasI AATT 6 cut(s) 428, 503, 526, 647, 895, 1202
TfiI GAWTC 6 cut(s) 159, 494, 515, 675, 743, 1300
Tru1I TTAA 4 cut(s) 650, 1016, 1116, 1205
Tru9I TTAA 4 cut(s) 650, 1016, 1116, 1205
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 1 cut(s) 827
Tsp45I GTSAC 1 cut(s) 1085
TspDTI ATGAA 6 cut(s) 15, 36, 170, 891, 908, 998
TspGWI ACGGA 1 cut(s) 229
XapI RAATTY 2 cut(s) 428, 895
XbaI TCTAGA 1 cut(s) 1303
XceI RCATGY 1 cut(s) 1227
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XhoI CTCGAG 1 cut(s) 1279
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 4 cut(s) 156, 447, 1211, 1304
Zsp2I ATGCAT 2 cut(s) 1057, 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.