MD03G1147700.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010796
Physical Location & Seq
Reverse (-)
16323806 .. 16329771
5966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1147700.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGACTGAGGTCCTCCATTCCCCTTCCCATTTTTCTTCCTCCCCAAGACCCAGCTCCTCTTCCTCTTCCTCTCCTCCTTCCAATTCTTCCTTATCCTGCGCAGCCCCGTCTTCTTTATCCGGCGCACCCCGGCCCACCGAAGACGACGACGATTGCGACTCTGCGGCGACTTGCTGTGAGGCGAAGGACCGTGACAAGCGCAGAAGGGAGCAGCTTTCTCTGTTGGCGTTGCTGGTAACCTTTTTCAGGAAATCTTTGATTCCTTGCAAGAGTGACAGGAGGGAGCTTTGCGCTATGGAGATCGGCTGGCCAACCAATGTGCGACACGTGGCACATGTAACCTTTGATAGGTTCAATGGGTTCTTGGGTTTGCCCGTGGAGTTTGAACCCGAAGTGCCCAGGAGACCTCCCAGTGCAAGTACAACTGTTTTTGGAGTATCCACGGAGTCCATGCAGTTGTCATACGACTCTAGAGGTAATAGTGTGCCAACAATTCTCTTGCTTATGCAAGGACGGTTATATGCAGAAGGAGGCCTTCAGGCGGAAGGGATTTTCAGAATTAATGCTGAAAACAGTCAAGAGGAGCATGTAAGGGACCAATTAAATAGGGGAGATGTACCAGAAGGTATTGATATACACTGTCTGGCAGGACTGATCAAGGCTTGGTTTAGGGAACTCCCAGCCGGGGTTCTGGATTCTCTATCGCCTGAGCAGGAACATCTAAACAAGATGAATGCACGCAACATAGCCATGGTTTTTGCACCAAACATGACTCAGATGGCAGATCCATTGACAGCATTGATGTATGCAGTCCAAGTGATGAACTTCCTGATAACACTTATTTTGAAGACACTGCGCGAAAGGAAGGATTTTGTTGTAGAGCCTCCTTTGTGCTATGTAGAACCCTCTGATGAGGATGGACACCAGAGCCCTTCACAATCCTTAAAGGAAGGTATTGGCAAACTTAATGAAGGGAAAGAGCAGTCGGTGTTCATGGAAGAACCTCAATCTGGAAGTTCCTCCAACTCTAATCAAGTCAGTGGGTGTTGCCATGATGTGGTGCAAGATGACTCGTTGGTTAATGAAATGGAAGCTGGCAGAGTCAACAACATGACTTCTGAAGTTCAAGCGCACGACTTCTCTAAGAGCGCGCCTGGTCAATCGAGTGATTCAAATATCAAAATGGTGCCTAAAAGACCAAGTGAACAGCAGTCTGTAATACGCGGAACAGGGCCTATTCAGAAGGCTAGGGGAATCAGCACTCTGAGTCGCATAGATTCAACGATGGAGCGGATTGAAGCCTGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

437

Amino Acids

47.98

Weight (kDa)

5.28

Isoelectric Point (pI)

67.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 177 - 231 2.4e-08 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 100
AccB1I GGYRCC 1 cut(s) 1186
AccB7I CCANNNNNTGG 1 cut(s) 1057
AccBSI CCGCTC 1 cut(s) 1291
AccII CGCG 3 cut(s) 858, 1151, 1224
AciI CCGC 5 cut(s) 164, 542, 1224, 1291, 1306
AclWI GGATC 1 cut(s) 779
AcoI YGGCCR 1 cut(s) 308
AcuI CTGAAG 2 cut(s) 521, 1140
AcvI CACGTG 1 cut(s) 328
AfaI GTAC 2 cut(s) 421, 618
AfiI CCNNNNNNNGG 6 cut(s) 246, 348, 541, 685, 1010, 1057
AflIII ACRYGT 2 cut(s) 325, 334
AgsI TTSAA 7 cut(s) 355, 386, 847, 1127, 1173, 1281, 1298
AjnI CCWGG 3 cut(s) 398, 1153, 1301
AluBI AGCT 4 cut(s) 54, 214, 286, 1094
AluI AGCT 4 cut(s) 54, 214, 286, 1094
Alw26I GTCTC 1 cut(s) 397
AlwI GGATC 1 cut(s) 779
AoxI GGCC 4 cut(s) 131, 308, 532, 1232
ApeKI GCWGC 2 cut(s) 101, 211
AseI ATTAAT 1 cut(s) 561
AspLEI GCGC 8 cut(s) 101, 125, 201, 293, 858, 1132, 1151, 1153
AspS9I GGNCC 5 cut(s) 10, 132, 187, 595, 1232
AsuC2I CCSGG 2 cut(s) 130, 685
AvaII GGWCC 3 cut(s) 10, 187, 595
BaeGI GKGCMC 1 cut(s) 399
BalI TGGCCA 1 cut(s) 310
BanI GGYRCC 1 cut(s) 1186
BanII GRGCYC 1 cut(s) 932
BbrPI CACGTG 1 cut(s) 328
BbsI GAAGAC 3 cut(s) 102, 147, 854
BbvI GCAGC 2 cut(s) 113, 223
BccI CCATC 3 cut(s) 772, 911, 1279
BciT130I CCWGG 3 cut(s) 400, 1155, 1303
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 1 cut(s) 654
BcnI CCSGG 2 cut(s) 130, 685
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 2 cut(s) 471, 1248
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 3 cut(s) 102, 165, 212
BlsI GCNGC 3 cut(s) 103, 166, 213
Bme1390I CCNGG 5 cut(s) 130, 400, 685, 1155, 1303
Bme18I GGWCC 3 cut(s) 10, 187, 595
BmgT120I GGNCC 5 cut(s) 10, 132, 187, 595, 1232
BmiI GGNNCC 2 cut(s) 596, 1188
BmrFI CCNGG 5 cut(s) 130, 400, 685, 1155, 1303
BmrI ACTGGG 1 cut(s) 405
BmuI ACTGGG 1 cut(s) 405
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 3 cut(s) 102, 147, 854
Bpu10I CCTNAGC 1 cut(s) 708
BpuMI CCSGG 2 cut(s) 130, 685
BsaAI YACGTR 1 cut(s) 328
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 6 cut(s) 128, 375, 398, 441, 684, 750
Bsc4I CCNNNNNNNGG 6 cut(s) 246, 348, 541, 685, 1010, 1057
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 3 cut(s) 400, 1155, 1303
BseDI CCNNGG 6 cut(s) 128, 375, 398, 441, 684, 750
BseGI GGATG 1 cut(s) 922
BseLI CCNNNNNNNGG 6 cut(s) 246, 348, 541, 685, 1010, 1057
BseMII CTCAG 3 cut(s) 699, 788, 1256
BseNI ACTGG 1 cut(s) 411
BsePI GCGCGC 1 cut(s) 1149
BseRI GAGGAG 3 cut(s) 46, 63, 596
BseSI GKGCMC 1 cut(s) 399
BseXI GCAGC 2 cut(s) 113, 223
BseYI CCCAGC 2 cut(s) 50, 679
Bsh1236I CGCG 3 cut(s) 858, 1151, 1224
BshFI GGCC 4 cut(s) 133, 310, 534, 1234
BshNI GGYRCC 1 cut(s) 1186
BsiSI CCGG 3 cut(s) 120, 130, 684
BslFI GGGAC 1 cut(s) 608
BslI CCNNNNNNNGG 6 cut(s) 246, 348, 541, 685, 1010, 1057
BsmAI GTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 608
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 4 cut(s) 133, 310, 534, 1234
Bso31I GGTCTC 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 399, 932
Bsp143I GATC 3 cut(s) 300, 654, 784
Bsp19I CCATGG 1 cut(s) 750
BspACI CCGC 5 cut(s) 164, 542, 1224, 1291, 1306
BspANI GGCC 4 cut(s) 133, 310, 534, 1234
BspCNI CTCAG 3 cut(s) 700, 787, 1257
BspFNI CGCG 3 cut(s) 858, 1151, 1224
BspLI GGNNCC 2 cut(s) 596, 1188
BspPI GGATC 1 cut(s) 779
BspT107I GGYRCC 1 cut(s) 1186
BspTNI GGTCTC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 1291
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 6 cut(s) 128, 375, 398, 441, 684, 750
BssHII GCGCGC 1 cut(s) 1149
BssMI GATC 3 cut(s) 300, 654, 784
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 3 cut(s) 400, 1155, 1303
Bst4CI ACNGT 5 cut(s) 191, 427, 516, 575, 641
Bst6I CTCTTC 2 cut(s) 64, 70
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 4 cut(s) 308, 739, 1096, 1151
BstDEI CTNAG 5 cut(s) 6, 708, 774, 1143, 1265
BstDSI CCRYGG 3 cut(s) 375, 441, 750
BstEII GGTNACC 1 cut(s) 235
BstENI CCTNNNNNAGG 2 cut(s) 244, 346
BstF5I GGATG 1 cut(s) 922
BstFNI CGCG 3 cut(s) 858, 1151, 1224
BstHHI GCGC 8 cut(s) 101, 125, 201, 293, 858, 1132, 1151, 1153
BstKTI GATC 3 cut(s) 303, 657, 787
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 3 cut(s) 300, 654, 784
BstNI CCWGG 3 cut(s) 400, 1155, 1303
BstNSI RCATGY 2 cut(s) 338, 590
BstPAI GACNNNNGTC 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 235
BstSCI CCNGG 5 cut(s) 128, 398, 683, 1153, 1301
BstSLI GKGCMC 1 cut(s) 399
BstUI CGCG 3 cut(s) 858, 1151, 1224
BstV1I GCAGC 2 cut(s) 113, 223
BstV2I GAAGAC 3 cut(s) 102, 147, 854
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 133, 310, 534, 1234
BtgI CCRYGG 3 cut(s) 375, 441, 750
BtsCI GGATG 1 cut(s) 922
BtsI GCAGTG 1 cut(s) 851
BtsIMutI CAGTG 4 cut(s) 418, 637, 851, 1045
Cac8I GCNNGC 4 cut(s) 308, 739, 1096, 1151
CfoI GCGC 8 cut(s) 101, 125, 201, 293, 858, 1132, 1151, 1153
Cfr13I GGNCC 5 cut(s) 10, 132, 187, 595, 1232
Csp6I GTAC 2 cut(s) 420, 617
CviAII CATG 8 cut(s) 335, 451, 587, 751, 769, 994, 1052, 1111
CviQI GTAC 2 cut(s) 420, 617
DdeI CTNAG 5 cut(s) 6, 708, 774, 1143, 1265
DpnI GATC 3 cut(s) 302, 656, 786
DpnII GATC 3 cut(s) 300, 654, 784
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 2 cut(s) 64, 70
EarI CTCTTC 2 cut(s) 64, 70
EciI GGCGGA 1 cut(s) 557
Eco130I CCWWGG 1 cut(s) 750
Eco147I AGGCCT 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 932
Eco31I GGTCTC 1 cut(s) 397
Eco47I GGWCC 3 cut(s) 10, 187, 595
Eco57I CTGAAG 2 cut(s) 521, 1140
Eco72I CACGTG 1 cut(s) 328
Eco91I GGTNACC 1 cut(s) 235
EcoNI CCTNNNNNAGG 2 cut(s) 244, 346
EcoO109I RGGNCCY 2 cut(s) 10, 1232
EcoO65I GGTNACC 1 cut(s) 235
EcoRII CCWGG 3 cut(s) 398, 1153, 1301
EcoT14I CCWWGG 1 cut(s) 750
EcoT38I GRGCYC 1 cut(s) 932
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 8 cut(s) 338, 454, 590, 754, 772, 997, 1055, 1114
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 1 cut(s) 608
FatI CATG 8 cut(s) 334, 450, 586, 750, 768, 993, 1051, 1110
FbaI TGATCA 1 cut(s) 654
Fnu4HI GCNGC 3 cut(s) 102, 165, 212
FokI GGATG 1 cut(s) 929
FriOI GRGCYC 1 cut(s) 932
Fsp4HI GCNGC 3 cut(s) 102, 165, 212
FspBI CTAG 2 cut(s) 471, 1248
FspI TGCGCA 1 cut(s) 100
GlaI GCGC 8 cut(s) 100, 124, 200, 292, 857, 1131, 1150, 1152
GluI GCNGC 3 cut(s) 102, 165, 212
GsaI CCCAGC 2 cut(s) 54, 683
HaeIII GGCC 4 cut(s) 133, 310, 534, 1234
HapII CCGG 3 cut(s) 120, 130, 684
HhaI GCGC 8 cut(s) 101, 125, 201, 293, 858, 1132, 1151, 1153
Hin1II CATG 8 cut(s) 338, 454, 590, 754, 772, 997, 1055, 1114
Hin6I GCGC 8 cut(s) 99, 123, 199, 291, 856, 1130, 1149, 1151
HinP1I GCGC 8 cut(s) 99, 123, 199, 291, 856, 1130, 1149, 1151
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HpaII CCGG 3 cut(s) 120, 130, 684
Hpy166II GTNNAC 2 cut(s) 1105, 1205
Hpy188I TCNGA 6 cut(s) 557, 777, 910, 1120, 1242, 1266
Hpy188III TCNNGA 6 cut(s) 247, 471, 578, 692, 829, 1011
Hpy8I GTNNAC 2 cut(s) 1105, 1205
Hpy99I CGWCG 2 cut(s) 149, 152
HpyCH4III ACNGT 5 cut(s) 191, 427, 516, 575, 641
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 9 cut(s) 267, 416, 454, 508, 524, 737, 761, 809, 1063
HpyF3I CTNAG 5 cut(s) 6, 708, 774, 1143, 1265
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 8 cut(s) 338, 454, 590, 754, 772, 997, 1055, 1114
HspAI GCGC 8 cut(s) 99, 123, 199, 291, 856, 1130, 1149, 1151
Ksp22I TGATCA 1 cut(s) 654
Kzo9I GATC 3 cut(s) 300, 654, 784
LmnI GCTCC 5 cut(s) 59, 208, 283, 583, 1288
Lsp1109I GCAGC 2 cut(s) 113, 223
MaeI CTAG 2 cut(s) 471, 1248
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 4 cut(s) 191, 235, 272, 337
MalI GATC 3 cut(s) 302, 656, 786
MbiI CCGCTC 1 cut(s) 1291
MboI GATC 3 cut(s) 300, 654, 784
MboII GAAGA 8 cut(s) 27, 51, 57, 78, 102, 152, 859, 1010
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 399, 932
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 4 cut(s) 82, 492, 558, 599
MluNI TGGCCA 1 cut(s) 310
MlyI GAGTC 7 cut(s) 152, 455, 461, 766, 1064, 1110, 1276
MmeI TCCRAC 1 cut(s) 1047
Mox20I TGGCCA 1 cut(s) 310
MscI TGGCCA 1 cut(s) 310
MseI TTAA 5 cut(s) 561, 602, 944, 966, 1080
MslI CAYNNNNRTG 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 310
MspI CCGG 3 cut(s) 120, 130, 684
MspR9I CCNGG 5 cut(s) 130, 400, 685, 1155, 1303
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 400, 1155, 1303
MvnI CGCG 3 cut(s) 858, 1151, 1224
NciI CCSGG 2 cut(s) 130, 685
NcoI CCATGG 1 cut(s) 750
NdeII GATC 3 cut(s) 300, 654, 784
NlaIII CATG 8 cut(s) 338, 454, 590, 754, 772, 997, 1055, 1114
NlaIV GGNNCC 2 cut(s) 596, 1188
NmuCI GTSAC 2 cut(s) 191, 272
NsbI TGCGCA 1 cut(s) 100
NspI RCATGY 2 cut(s) 338, 590
PauI GCGCGC 1 cut(s) 1149
PceI AGGCCT 1 cut(s) 534
PciI ACATGT 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 5 cut(s) 259, 695, 1169, 1254, 1277
PflMI CCANNNNNTGG 1 cut(s) 1057
PkrI GCNGC 3 cut(s) 103, 166, 213
PleI GAGTC 7 cut(s) 152, 454, 461, 766, 1064, 1109, 1275
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 7 cut(s) 152, 454, 461, 766, 1064, 1109, 1275
Ppu21I YACGTR 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 10
PscI ACATGT 1 cut(s) 334
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 3 cut(s) 398, 1153, 1301
PspCI CACGTG 1 cut(s) 328
PspEI GGTNACC 1 cut(s) 235
PspFI CCCAGC 2 cut(s) 50, 679
PspGI CCWGG 3 cut(s) 398, 1153, 1301
PspN4I GGNNCC 2 cut(s) 596, 1188
PspPI GGNCC 5 cut(s) 10, 132, 187, 595, 1232
PspPPI RGGWCCY 1 cut(s) 10
PsuI RGATCY 1 cut(s) 784
PteI GCGCGC 1 cut(s) 1149
RsaI GTAC 2 cut(s) 421, 618
RsaNI GTAC 2 cut(s) 420, 617
RseI CAYNNNNRTG 1 cut(s) 749
SaqAI TTAA 5 cut(s) 561, 602, 944, 966, 1080
SatI GCNGC 3 cut(s) 102, 165, 212
Sau3AI GATC 3 cut(s) 300, 654, 784
Sau96I GGNCC 5 cut(s) 10, 132, 187, 595, 1232
SchI GAGTC 7 cut(s) 152, 455, 461, 766, 1064, 1110, 1276
ScrFI CCNGG 5 cut(s) 130, 400, 685, 1155, 1303
SduI GDGCHC 2 cut(s) 399, 932
SinI GGWCC 3 cut(s) 10, 187, 595
SmiMI CAYNNNNRTG 1 cut(s) 749
Sse9I AATT 4 cut(s) 82, 492, 558, 599
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 5 cut(s) 164, 542, 1224, 1291, 1306
SspMI CTAG 2 cut(s) 471, 1248
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 5 cut(s) 128, 398, 683, 1153, 1301
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 5 cut(s) 191, 427, 516, 575, 641
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 1163
TasI AATT 4 cut(s) 82, 492, 558, 599
TatI WGTACW 1 cut(s) 419
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 5 cut(s) 259, 695, 1169, 1254, 1277
Tru1I TTAA 5 cut(s) 561, 602, 944, 966, 1080
Tru9I TTAA 5 cut(s) 561, 602, 944, 966, 1080
TscAI CASTG 4 cut(s) 418, 644, 858, 1045
TseFI GTSAC 2 cut(s) 191, 272
TseI GCWGC 2 cut(s) 101, 211
Tsp45I GTSAC 2 cut(s) 191, 272
TspDTI ATGAA 5 cut(s) 746, 836, 982, 984, 1098
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 4 cut(s) 418, 644, 858, 1045
Van91I CCANNNNNTGG 1 cut(s) 1057
VpaK11BI GGWCC 3 cut(s) 10, 187, 595
VspI ATTAAT 1 cut(s) 561
XagI CCTNNNNNAGG 2 cut(s) 244, 346
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 2 cut(s) 338, 590
XspI CTAG 2 cut(s) 471, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.