MD11G1166500.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010796
Physical Location & Seq
Reverse (-)
17629253 .. 17635400
6148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1166500.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGACAGAGGTTCTCCATTCCCCTTCCCATTTTTGTTCCTCCCCAAGACCCAGCTCCTCTTCCCCGCTTCCTCCTTCCAATTCTTCATCTGTCTCCTTGTCCTGCGCACCCCCGTCTTCTTTGTCCGGGGCACCCCCGCGCAGCAAAGACGACGACGATTGCGAGTCTGCGGTGACTGACTGTGAGGCGAAGGACCGTGACAAGCGCAGAAGGGAGCAGCTTTCTCTGTTGGCGTTGCTGGTCACCTTTTTCAGGAAATCTTTGATTCCGTGCAGGAGTGACAGGAGGGAGCTTTGCGCTATGGAGATCGGATTGCCAACCAATGTGCGGCACGTTGCCCATGTCACCTTTGATAGGTTCAATGGGTTCTTGGGTTTGCCCGTGGAGTTTGAACCTGAAGTGCCCAGTAGGCCTCCCAGTGCAAGTACAACTGTTTTTGGAGTTTCCACGGAGTCCATGCAGCTGTCATACGACTCTAGAGGTAATAGTGTGCCAACAATTCTCTTGCTTATGCAAGGACGGTTATATGCTGAAGGAGGCCTTCAGGCAGAAGGTATTTTCAGAATTAATGCTGAAAACAGTCAAGAGGAGCATGTAAGGGACCAATTAAATAGGGGAGATGTACCAGAAGGCATCGATATACACTGTTTGGCAGGACTGATCAAGGCTTGGTTTAGGGAACTCCCGGCTGGGGTTCTGGACTCTCTATCGCCTGAGCAGGTAATGCAATGCCAGACTGAAGTTGACTGTTCACAACTTGTGAGGCTTCTGCCGCCGACTGAAGCTTCTCTACTGGATTGGGCCATCAACCTTATGGCTGATGTTGTTCAACAGGAACATCTAAACAAGATGAATGCACGCAACATAGCCATGGTTTTTGCACCAAACATGACTCAGATGGCAGATCCATTGACTGCATTGATGTATGCAGTCCAAGTGATGAACTTCCTCAAAACACTTATTTTGAAGACATTGCGAGAAAGAAAGGATTCTGTGGTAGAGCCTCCCGTGTGCAATGTAGAGCCCTCTGACGATAATGGACACCAGAGCCCTTCCCAGTCCTTCGAGGAAGATATTGGCAAATGTAACCAAGAGAAAGAGCAGTCAGTGATTACTGAAGGACCTCAATCTGAAAGTTCCTCCATCTCTAACCGAGTCAGTAACCTAACCAATAGAGAGGCTAGCAGTTCGGTGAGCCTGGTTAAGAAGCTAAATCCTGACGGCAGTGGGTGTTGCGAGAATGCAGTGCAAGATGACTACTTGGTTAATGAAATGGAAGCTGGCAGAGTCAACAACATGACATCTGAAGTTCAAGCGGACACACCTGGTCAGTCGAGTAATTTAACTATCAAAACGGGGCCTAAACAACTAAGTGATCAGAAGTCTGTAATACGGGGAACAGGGCTTATTGAGAAGGCTAGGGGAATCAGCACTCTGAGTCGCATAGATTCAACGATGGAGCGGATTGAAGCCTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

494

Amino Acids

54.12

Weight (kDa)

5.02

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 179 - 299 1.4e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 709
AccB1I GGYRCC 1 cut(s) 130
AccBSI CCGCTC 1 cut(s) 1462
AccII CGCG 1 cut(s) 139
AciI CCGC 7 cut(s) 65, 137, 170, 328, 773, 1316, 1462
AclWI GGATC 1 cut(s) 899
AcuI CTGAAG 7 cut(s) 417, 527, 552, 759, 801, 1137, 1326
AfaI GTAC 2 cut(s) 427, 624
AfiI CCNNNNNNNGG 4 cut(s) 252, 327, 354, 691
AgsI TTSAA 7 cut(s) 361, 392, 830, 967, 1313, 1452, 1469
AjnI CCWGG 3 cut(s) 1197, 1324, 1472
AluBI AGCT 7 cut(s) 54, 220, 292, 463, 785, 1210, 1280
AluI AGCT 7 cut(s) 54, 220, 292, 463, 785, 1210, 1280
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 1 cut(s) 899
AoxI GGCC 4 cut(s) 410, 538, 801, 1358
ApeKI GCWGC 3 cut(s) 141, 217, 460
AseI ATTAAT 1 cut(s) 567
Asp700I GAANNNNTTC 1 cut(s) 988
AspLEI GCGC 4 cut(s) 107, 141, 207, 299
AspS9I GGNCC 5 cut(s) 193, 601, 801, 1121, 1358
AsuC2I CCSGG 2 cut(s) 127, 686
AsuHPI GGTGA 4 cut(s) 184, 235, 337, 1204
AsuNHI GCTAGC 1 cut(s) 1181
AvaII GGWCC 3 cut(s) 193, 601, 1121
BaeGI GKGCMC 2 cut(s) 133, 405
BanI GGYRCC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 1026, 1052
BarI GAAGNNNNNNTAC 2 cut(s) 774, 806
BbsI GAAGAC 2 cut(s) 108, 974
BbvI GCAGC 3 cut(s) 153, 229, 472
BccI CCATC 4 cut(s) 812, 892, 1151, 1450
BceAI ACGGC 1 cut(s) 1237
BciT130I CCWGG 3 cut(s) 1199, 1326, 1474
BclI TGATCA 2 cut(s) 660, 1375
BcnI CCSGG 2 cut(s) 127, 686
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 3 cut(s) 477, 1182, 1419
BfuAI ACCTGC 1 cut(s) 709
BglI GCCNNNNNGGC 1 cut(s) 409
BisI GCNGC 5 cut(s) 142, 218, 329, 461, 773
BlsI GCNGC 5 cut(s) 143, 219, 330, 462, 774
Bme1390I CCNGG 5 cut(s) 127, 686, 1199, 1326, 1474
Bme18I GGWCC 3 cut(s) 193, 601, 1121
BmgT120I GGNCC 5 cut(s) 193, 601, 801, 1121, 1358
BmiI GGNNCC 3 cut(s) 132, 602, 1359
BmrFI CCNGG 5 cut(s) 127, 686, 1199, 1326, 1474
BmrI ACTGGG 3 cut(s) 399, 411, 1051
BmsI GCATC 1 cut(s) 642
BmtI GCTAGC 1 cut(s) 1185
BmuI ACTGGG 3 cut(s) 399, 411, 1051
BpiI GAAGAC 2 cut(s) 108, 974
Bpu10I CCTNAGC 1 cut(s) 714
BpuMI CCSGG 2 cut(s) 127, 686
Bsa29I ATCGAT 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 126, 381, 447, 870
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 327, 354, 691
Bse1I ACTGG 4 cut(s) 405, 417, 798, 1057
Bse3DI GCAATG 3 cut(s) 734, 971, 1021
BseBI CCWGG 3 cut(s) 1199, 1326, 1474
BseCI ATCGAT 1 cut(s) 636
BseDI CCNNGG 4 cut(s) 126, 381, 447, 870
BseLI CCNNNNNNNGG 4 cut(s) 252, 327, 354, 691
BseMI GCAATG 3 cut(s) 734, 971, 1021
BseMII CTCAG 3 cut(s) 705, 908, 1427
BseNI ACTGG 4 cut(s) 405, 417, 798, 1057
BseRI GAGGAG 2 cut(s) 46, 602
BseSI GKGCMC 2 cut(s) 133, 405
BseXI GCAGC 3 cut(s) 153, 229, 472
BseYI CCCAGC 2 cut(s) 50, 689
BsgI GTGCAG 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 4 cut(s) 412, 540, 803, 1360
BshNI GGYRCC 1 cut(s) 130
BshVI ATCGAT 1 cut(s) 636
BsiSI CCGG 2 cut(s) 126, 686
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 4 cut(s) 252, 327, 354, 691
BsmAI GTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 614
BsmI GAATGC 2 cut(s) 859, 1246
BsnI GGCC 4 cut(s) 412, 540, 803, 1360
Bsp1286I GDGCHC 4 cut(s) 133, 405, 1026, 1052
Bsp143I GATC 4 cut(s) 306, 660, 904, 1375
Bsp19I CCATGG 1 cut(s) 870
BspACI CCGC 7 cut(s) 65, 137, 170, 328, 773, 1316, 1462
BspANI GGCC 4 cut(s) 412, 540, 803, 1360
BspCNI CTCAG 3 cut(s) 706, 907, 1428
BspDI ATCGAT 1 cut(s) 636
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 132, 602, 1359
BspMI ACCTGC 1 cut(s) 709
BspOI GCTAGC 1 cut(s) 1185
BspPI GGATC 1 cut(s) 899
BspT107I GGYRCC 1 cut(s) 130
BsrBI CCGCTC 1 cut(s) 1462
BsrDI GCAATG 3 cut(s) 734, 971, 1021
BsrI ACTGG 4 cut(s) 405, 417, 798, 1057
BssECI CCNNGG 4 cut(s) 126, 381, 447, 870
BssMI GATC 4 cut(s) 306, 660, 904, 1375
BssT1I CCWWGG 1 cut(s) 870
Bst2UI CCWGG 3 cut(s) 1199, 1326, 1474
Bst4CI ACNGT 7 cut(s) 182, 197, 433, 522, 581, 647, 749
Bst6I CTCTTC 1 cut(s) 64
BstAPI GCANNNNNTGC 1 cut(s) 724
BstC8I GCNNGC 3 cut(s) 859, 1183, 1282
BstDEI CTNAG 4 cut(s) 714, 894, 1370, 1436
BstDSI CCRYGG 3 cut(s) 381, 447, 870
BstEII GGTNACC 1 cut(s) 241
BstENI CCTNNNNNAGG 2 cut(s) 250, 352
BstFNI CGCG 1 cut(s) 139
BstHHI GCGC 4 cut(s) 107, 141, 207, 299
BstKTI GATC 4 cut(s) 309, 663, 907, 1378
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 4 cut(s) 306, 660, 904, 1375
BstMWI GCNNNNNNNGC 3 cut(s) 409, 724, 772
BstNI CCWGG 3 cut(s) 1199, 1326, 1474
BstNSI RCATGY 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 241
BstSCI CCNGG 5 cut(s) 125, 684, 1197, 1324, 1472
BstSLI GKGCMC 2 cut(s) 133, 405
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 153, 229, 472
BstV2I GAAGAC 2 cut(s) 108, 974
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
Bsu15I ATCGAT 1 cut(s) 636
BsuRI GGCC 4 cut(s) 412, 540, 803, 1360
BsuTUI ATCGAT 1 cut(s) 636
BtgI CCRYGG 3 cut(s) 381, 447, 870
BtsI GCAGTG 2 cut(s) 1231, 1251
BtsIMutI CAGTG 5 cut(s) 424, 643, 1113, 1231, 1251
BveI ACCTGC 1 cut(s) 709
Cac8I GCNNGC 3 cut(s) 859, 1183, 1282
CfoI GCGC 4 cut(s) 107, 141, 207, 299
Cfr13I GGNCC 5 cut(s) 193, 601, 801, 1121, 1358
ClaI ATCGAT 1 cut(s) 636
CsiI ACCWGGT 1 cut(s) 1324
Csp6I GTAC 2 cut(s) 426, 623
CviAII CATG 6 cut(s) 341, 457, 593, 871, 889, 1297
CviQI GTAC 2 cut(s) 426, 623
DdeI CTNAG 4 cut(s) 714, 894, 1370, 1436
DpnI GATC 4 cut(s) 308, 662, 906, 1377
DpnII GATC 4 cut(s) 306, 660, 904, 1375
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 870
Eco147I AGGCCT 2 cut(s) 412, 540
Eco24I GRGCYC 2 cut(s) 1026, 1052
Eco47I GGWCC 3 cut(s) 193, 601, 1121
Eco57I CTGAAG 7 cut(s) 417, 527, 552, 759, 801, 1137, 1326
Eco91I GGTNACC 1 cut(s) 241
EcoNI CCTNNNNNAGG 2 cut(s) 250, 352
EcoO109I RGGNCCY 2 cut(s) 1121, 1358
EcoO65I GGTNACC 1 cut(s) 241
EcoRII CCWGG 3 cut(s) 1197, 1324, 1472
EcoT14I CCWWGG 1 cut(s) 870
EcoT38I GRGCYC 2 cut(s) 1026, 1052
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 6 cut(s) 344, 460, 596, 874, 892, 1300
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 1 cut(s) 614
FatI CATG 6 cut(s) 340, 456, 592, 870, 888, 1296
FauI CCCGC 2 cut(s) 72, 144
FbaI TGATCA 2 cut(s) 660, 1375
Fnu4HI GCNGC 5 cut(s) 142, 218, 329, 461, 773
FriOI GRGCYC 2 cut(s) 1026, 1052
Fsp4HI GCNGC 5 cut(s) 142, 218, 329, 461, 773
FspBI CTAG 3 cut(s) 477, 1182, 1419
FspI TGCGCA 1 cut(s) 106
GlaI GCGC 4 cut(s) 106, 140, 206, 298
GluI GCNGC 5 cut(s) 142, 218, 329, 461, 773
GsaI CCCAGC 2 cut(s) 54, 693
HaeIII GGCC 4 cut(s) 412, 540, 803, 1360
HapII CCGG 2 cut(s) 126, 686
HhaI GCGC 4 cut(s) 107, 141, 207, 299
Hin1II CATG 6 cut(s) 344, 460, 596, 874, 892, 1300
Hin6I GCGC 4 cut(s) 105, 139, 205, 297
HinP1I GCGC 4 cut(s) 105, 139, 205, 297
HincII GTYRAC 2 cut(s) 745, 1291
HindII GTYRAC 2 cut(s) 745, 1291
HindIII AAGCTT 1 cut(s) 783
HpaII CCGG 2 cut(s) 126, 686
HphI GGTGA 4 cut(s) 184, 235, 337, 1204
Hpy166II GTNNAC 3 cut(s) 745, 752, 1291
Hpy188I TCNGA 8 cut(s) 311, 563, 897, 1030, 1132, 1306, 1380, 1437
Hpy188III TCNNGA 5 cut(s) 253, 477, 584, 698, 1217
Hpy8I GTNNAC 3 cut(s) 745, 752, 1291
Hpy99I CGWCG 2 cut(s) 155, 158
HpyCH4III ACNGT 7 cut(s) 182, 197, 433, 522, 581, 647, 749
HpyCH4IV ACGT 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 409, 724, 772
HpyF3I CTNAG 4 cut(s) 714, 894, 1370, 1436
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 6 cut(s) 344, 460, 596, 874, 892, 1300
HspAI GCGC 4 cut(s) 105, 139, 205, 297
Ksp22I TGATCA 2 cut(s) 660, 1375
Kzo9I GATC 4 cut(s) 306, 660, 904, 1375
LmnI GCTCC 5 cut(s) 59, 214, 289, 589, 1459
Lsp1109I GCAGC 3 cut(s) 153, 229, 472
LweI GCATC 1 cut(s) 642
MabI ACCWGGT 1 cut(s) 1324
MaeI CTAG 3 cut(s) 477, 1182, 1419
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 7 cut(s) 172, 197, 241, 278, 343, 1085, 1160
MalI GATC 4 cut(s) 308, 662, 906, 1377
MbiI CCGCTC 1 cut(s) 1462
MboI GATC 4 cut(s) 306, 660, 904, 1375
MboII GAAGA 5 cut(s) 51, 75, 108, 979, 1082
MflI RGATCY 1 cut(s) 904
MhlI GDGCHC 4 cut(s) 133, 405, 1026, 1052
MluCI AATT 5 cut(s) 79, 498, 564, 605, 1339
MlyI GAGTC 8 cut(s) 173, 461, 467, 695, 886, 1164, 1296, 1447
MroXI GAANNNNTTC 1 cut(s) 988
MseI TTAA 5 cut(s) 567, 608, 1203, 1266, 1343
MslI CAYNNNNRTG 1 cut(s) 869
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 2 cut(s) 126, 686
MspR9I CCNGG 5 cut(s) 127, 686, 1199, 1326, 1474
Mva1269I GAATGC 2 cut(s) 859, 1246
MvaI CCWGG 3 cut(s) 1199, 1326, 1474
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 409, 724, 772
NciI CCSGG 2 cut(s) 127, 686
NcoI CCATGG 1 cut(s) 870
NdeII GATC 4 cut(s) 306, 660, 904, 1375
NheI GCTAGC 1 cut(s) 1181
NlaIII CATG 6 cut(s) 344, 460, 596, 874, 892, 1300
NlaIV GGNNCC 3 cut(s) 132, 602, 1359
NmuCI GTSAC 5 cut(s) 172, 197, 241, 278, 343
NsbI TGCGCA 1 cut(s) 106
NspI RCATGY 1 cut(s) 596
PceI AGGCCT 2 cut(s) 412, 540
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 2 cut(s) 859, 1246
PdmI GAANNNNTTC 1 cut(s) 988
PfeI GAWTC 4 cut(s) 265, 989, 1425, 1448
PkrI GCNGC 5 cut(s) 143, 219, 330, 462, 774
PleI GAGTC 8 cut(s) 172, 460, 467, 695, 886, 1163, 1295, 1446
PpsI GAGTC 8 cut(s) 172, 460, 467, 695, 886, 1163, 1295, 1446
PpuMI RGGWCCY 1 cut(s) 1121
PshBI ATTAAT 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 1121
Psp6I CCWGG 3 cut(s) 1197, 1324, 1472
PspEI GGTNACC 1 cut(s) 241
PspFI CCCAGC 2 cut(s) 50, 689
PspGI CCWGG 3 cut(s) 1197, 1324, 1472
PspN4I GGNNCC 3 cut(s) 132, 602, 1359
PspPI GGNCC 5 cut(s) 193, 601, 801, 1121, 1358
PspPPI RGGWCCY 1 cut(s) 1121
PsuI RGATCY 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 2 cut(s) 427, 624
RsaNI GTAC 2 cut(s) 426, 623
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 5 cut(s) 567, 608, 1203, 1266, 1343
SatI GCNGC 5 cut(s) 142, 218, 329, 461, 773
Sau3AI GATC 4 cut(s) 306, 660, 904, 1375
Sau96I GGNCC 5 cut(s) 193, 601, 801, 1121, 1358
SchI GAGTC 8 cut(s) 173, 461, 467, 695, 886, 1164, 1296, 1447
ScrFI CCNGG 5 cut(s) 127, 686, 1199, 1326, 1474
SduI GDGCHC 4 cut(s) 133, 405, 1026, 1052
SexAI ACCWGGT 1 cut(s) 1324
SfaNI GCATC 1 cut(s) 642
SinI GGWCC 3 cut(s) 193, 601, 1121
SmiMI CAYNNNNRTG 1 cut(s) 869
Sse9I AATT 5 cut(s) 79, 498, 564, 605, 1339
SseBI AGGCCT 2 cut(s) 412, 540
SsiI CCGC 7 cut(s) 65, 137, 170, 328, 773, 1316, 1462
SspMI CTAG 3 cut(s) 477, 1182, 1419
StuI AGGCCT 2 cut(s) 412, 540
StyD4I CCNGG 5 cut(s) 125, 684, 1197, 1324, 1472
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 7 cut(s) 182, 197, 433, 522, 581, 647, 749
TaiI ACGT 1 cut(s) 336
TaqI TCGA 3 cut(s) 636, 1065, 1334
TasI AATT 5 cut(s) 79, 498, 564, 605, 1339
TatI WGTACW 1 cut(s) 425
TauI GCSGC 2 cut(s) 331, 775
TfiI GAWTC 4 cut(s) 265, 989, 1425, 1448
Tru1I TTAA 5 cut(s) 567, 608, 1203, 1266, 1343
Tru9I TTAA 5 cut(s) 567, 608, 1203, 1266, 1343
TscAI CASTG 5 cut(s) 424, 650, 1113, 1231, 1251
TseFI GTSAC 5 cut(s) 172, 197, 241, 278, 343
TseI GCWGC 3 cut(s) 141, 217, 460
Tsp45I GTSAC 5 cut(s) 172, 197, 241, 278, 343
TspDTI ATGAA 4 cut(s) 75, 866, 956, 1284
TspGWI ACGGA 2 cut(s) 258, 464
TspRI CASTG 5 cut(s) 424, 650, 1113, 1231, 1251
VpaK11BI GGWCC 3 cut(s) 193, 601, 1121
VspI ATTAAT 1 cut(s) 567
XagI CCTNNNNNAGG 2 cut(s) 250, 352
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 1 cut(s) 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 811
XmnI GAANNNNTTC 1 cut(s) 988
XspI CTAG 3 cut(s) 477, 1182, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.