Rh5CG340100

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010796
Physical Location & Seq
Reverse (-)
38960368 .. 38964514
4147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG340100.1

Sequence Viewer

Length: 1347 bp
ATGACAGAGCTCGTCCTCCATTCTCGTCCTGACCATTTCTCTTCATCTCCAACACCCACCACCTCCACCTCTTCTTCTTCTTCTCCACCACCCTCCTTAACCTCCGCACCGCCGTCTTCTTTAACCTGCGCACCCCCGAGGGACGAGTCTTGGGTGACGGACTGTGACGCCCGGCAGCTGTCCCTGTTGGCATTGCTGGTCACGTTTTTCAGGAAGTCTTTGAGTCCTGGCAAGAGGGAAAGGAGGGAGCTTTGTGCCATGGAGATCGGCTGGCCTACCAATGTGCGCCACGTGGCACATGTCACCTTTGACAGGTTCAATGGCTTCTTGGGTTTGCCTGTTGAGTTTGAGCCTGAACTTCCCAGTAGGCCTCCGAGTGCAAGTCGAACAGTTTTTGGAGTATCTACCAAATCCATGCAGTTGTCGTATGACTCCAGAGGGAATAGTGTGCCGACAATTCTCTTGCTTATGCAAGGGCGGTTGTATGCTGAAGGAGGTCTACAGGTAGAAGGGATATTCAGAATTAATGGAGAAAACAGTCAAGAGGAGCATATAAGAGGTCAATTAAACAGGGGGGATGTACCAGAAGGCATTGATATACACTGCTTGGCAGGGCTGATCAAGGCTTGGTTTAGGGAACTCCCAGTTGGGCTTCTGGATTCTCTAGCCCCTGAGCAGGTAATGCAGTGCCAGACAGAAGATGACTGCTTAGAGCTTGTGAGGCTTCTACCCTCTACCGAAGTTGCTCTATTGGATTGGGCGGTCAACCTTATGGCTGATGTTGTCGAACACGAACATCTAAACAAGATGAATGCGCGCAACATTGCCATGGTTTTTGCACCGAACATGACTCAGATGGCAGATCCTTTGACTGCACTGATGTATGCTGTTCAAGTGATGAACTTCCTCAAGATTCTTATTCTGAAGACACTGCGAGAAAGGAAACAAAATGTGGTAGAGGCCGTTATCTTTTGTGTAGAACCTGCTGATGAGAATGGACACCAGAGCTGTTCGCAATCATGCCATGAAGATGTTGTCAAAGATAATGAAGAGAGCCTGATTGAGAACCTAATTCCTAATGGCAGGGACTGTTGTGGTACTTCTGATCAAGTTGACACTTTGGGTAATGAAATGGAAGCTGTAAACACCATGACAATTGAAGTTCAAGGGGACTCTGGGAAGAGCGAAACTGATCAATTGAATAATTCAACTTTCAAAATGAGACCTGAAAGAATAAGTGATCAGCAAGCTGCAGTATGGGGAACAGGGCCCGTTGAGAAGGCTAGGAAAATCAGCAACCTGAGTCGCATAGATTCAAGGAGGGAAATAATTGAAGCCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

448

Amino Acids

49.78

Weight (kDa)

5.03

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 165 - 285 3.3e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 3 cut(s) 134, 667, 991
AccI GTMKAC 1 cut(s) 499
AccII CGCG 1 cut(s) 817
AciI CCGC 5 cut(s) 105, 110, 478, 761, 1342
AclWI GGATC 1 cut(s) 857
AcuI CTGAAG 2 cut(s) 510, 944
AcvI CACGTG 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 582, 1099
AfiI CCNNNNNNNGG 2 cut(s) 312, 676
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 9 cut(s) 319, 893, 1160, 1166, 1201, 1209, 1216, 1317, 1334
AjnI CCWGG 2 cut(s) 226, 1337
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AluBI AGCT 7 cut(s) 10, 178, 250, 715, 1008, 1139, 1250
AluI AGCT 7 cut(s) 10, 178, 250, 715, 1008, 1139, 1250
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 1 cut(s) 1216
AlwI GGATC 1 cut(s) 857
AlwNI CAGNNNCTG 1 cut(s) 1089
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 4 cut(s) 272, 368, 960, 1268
ApaI GGGCCC 1 cut(s) 1272
ApeKI GCWGC 2 cut(s) 175, 1250
AseI ATTAAT 1 cut(s) 525
AspLEI GCGC 4 cut(s) 131, 288, 817, 819
AspS9I GGNCC 2 cut(s) 1268, 1269
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 166, 295
AvaI CYCGRG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 1272
BanII GRGCYC 2 cut(s) 12, 1272
BarI GAAGNNNNNNTAC 2 cut(s) 483, 515
BbrPI CACGTG 1 cut(s) 292
BbsI GAAGAC 2 cut(s) 108, 932
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 187, 1237
BccI CCATC 1 cut(s) 850
BceAI ACGGC 2 cut(s) 97, 947
BciT130I CCWGG 2 cut(s) 228, 1339
BclI TGATCA 4 cut(s) 618, 1105, 1192, 1240
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 1216
BfaI CTAG 2 cut(s) 665, 1284
BfmI CTRYAG 2 cut(s) 500, 1251
BfuAI ACCTGC 3 cut(s) 134, 667, 991
BisI GCNGC 2 cut(s) 176, 1251
BlsI GCNGC 2 cut(s) 177, 1252
Bme1390I CCNGG 3 cut(s) 172, 228, 1339
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 1268, 1269
BmiI GGNNCC 1 cut(s) 1270
BmrFI CCNGG 3 cut(s) 172, 228, 1339
BmrI ACTGGG 2 cut(s) 357, 638
BmuI ACTGGG 2 cut(s) 357, 638
BpiI GAAGAC 2 cut(s) 108, 932
BpmI CTGGAG 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 893
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 1216
BsaJI CCNNGG 3 cut(s) 137, 258, 828
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 676
Bse1I ACTGG 2 cut(s) 363, 644
Bse3DI GCAATG 2 cut(s) 191, 822
BseBI CCWGG 2 cut(s) 228, 1339
BseDI CCNNGG 3 cut(s) 137, 258, 828
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 2 cut(s) 312, 676
BseMI GCAATG 2 cut(s) 191, 822
BseMII CTCAG 3 cut(s) 663, 866, 1292
BseNI ACTGG 2 cut(s) 363, 644
BsePI GCGCGC 1 cut(s) 815
BseRI GAGGAG 1 cut(s) 560
BseSI GKGCMC 1 cut(s) 1272
BseXI GCAGC 2 cut(s) 187, 1237
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 817
BshFI GGCC 4 cut(s) 274, 370, 962, 1270
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 172
BslFI GGGAC 4 cut(s) 155, 166, 1100, 1184
BslI CCNNNNNNNGG 2 cut(s) 312, 676
BsmAI GTCTC 1 cut(s) 1216
BsmFI GGGAC 4 cut(s) 155, 166, 1100, 1184
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 4 cut(s) 274, 370, 962, 1270
Bso31I GGTCTC 1 cut(s) 1216
BsoBI CYCGRG 1 cut(s) 136
Bsp120I GGGCCC 1 cut(s) 1268
Bsp1286I GDGCHC 2 cut(s) 12, 1272
Bsp143I GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
Bsp19I CCATGG 2 cut(s) 258, 828
BspACI CCGC 5 cut(s) 105, 110, 478, 761, 1342
BspANI GGCC 4 cut(s) 274, 370, 962, 1270
BspCNI CTCAG 3 cut(s) 664, 865, 1293
BspFNI CGCG 1 cut(s) 817
BspLI GGNNCC 1 cut(s) 1270
BspMAI CTGCAG 1 cut(s) 1255
BspMI ACCTGC 3 cut(s) 134, 667, 991
BspPI GGATC 1 cut(s) 857
BspQI GCTCTTC 1 cut(s) 1175
BspTNI GGTCTC 1 cut(s) 1216
BsrDI GCAATG 2 cut(s) 191, 822
BsrI ACTGG 2 cut(s) 363, 644
BssECI CCNNGG 3 cut(s) 137, 258, 828
BssHII GCGCGC 1 cut(s) 815
BssMI GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 2 cut(s) 258, 828
Bst2UI CCWGG 2 cut(s) 228, 1339
Bst4CI ACNGT 4 cut(s) 164, 391, 539, 1091
Bst6I CTCTTC 4 cut(s) 46, 76, 1044, 1175
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 3 cut(s) 272, 817, 1248
BstDEI CTNAG 4 cut(s) 672, 709, 852, 1301
BstDSI CCRYGG 2 cut(s) 258, 828
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 1 cut(s) 583
BstFNI CGCG 1 cut(s) 817
BstHHI GCGC 4 cut(s) 131, 288, 817, 819
BstKTI GATC 6 cut(s) 267, 621, 865, 1108, 1195, 1243
BstMAI GTCTC 1 cut(s) 1216
BstMBI GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
BstMWI GCNNNNNNNGC 2 cut(s) 682, 721
BstNI CCWGG 2 cut(s) 228, 1339
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 3 cut(s) 170, 226, 1337
BstSFI CTRYAG 2 cut(s) 500, 1251
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 1 cut(s) 817
BstV1I GCAGC 2 cut(s) 187, 1237
BstV2I GAAGAC 2 cut(s) 108, 932
BstX2I RGATCY 1 cut(s) 862
BstYI RGATCY 1 cut(s) 862
BsuRI GGCC 4 cut(s) 274, 370, 962, 1270
BtgI CCRYGG 2 cut(s) 258, 828
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 3 cut(s) 601, 692, 929
BtsIMutI CAGTG 4 cut(s) 601, 692, 875, 929
BveI ACCTGC 3 cut(s) 134, 667, 991
Cac8I GCNNGC 3 cut(s) 272, 817, 1248
CaiI CAGNNNCTG 1 cut(s) 1089
CfoI GCGC 4 cut(s) 131, 288, 817, 819
Cfr13I GGNCC 2 cut(s) 1268, 1269
CseI GACGC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 581, 1098
CviAII CATG 8 cut(s) 259, 299, 415, 829, 847, 1020, 1025, 1150
CviQI GTAC 2 cut(s) 581, 1098
DdeI CTNAG 4 cut(s) 672, 709, 852, 1301
DpnI GATC 6 cut(s) 266, 620, 864, 1107, 1194, 1242
DpnII GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
Eam1104I CTCTTC 4 cut(s) 46, 76, 1044, 1175
EarI CTCTTC 4 cut(s) 46, 76, 1044, 1175
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 258, 828
Eco147I AGGCCT 1 cut(s) 370
Eco24I GRGCYC 2 cut(s) 12, 1272
Eco31I GGTCTC 1 cut(s) 1216
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 510, 944
Eco72I CACGTG 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRII CCWGG 2 cut(s) 226, 1337
EcoT14I CCWWGG 2 cut(s) 258, 828
EcoT38I GRGCYC 2 cut(s) 12, 1272
ErhI CCWWGG 2 cut(s) 258, 828
FaeI CATG 8 cut(s) 262, 302, 418, 832, 850, 1023, 1028, 1153
FaqI GGGAC 4 cut(s) 155, 166, 1100, 1184
FatI CATG 8 cut(s) 258, 298, 414, 828, 846, 1019, 1024, 1149
FbaI TGATCA 4 cut(s) 618, 1105, 1192, 1240
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 2 cut(s) 176, 1251
FokI GGATG 1 cut(s) 590
FriOI GRGCYC 2 cut(s) 12, 1272
Fsp4HI GCNGC 2 cut(s) 176, 1251
FspBI CTAG 2 cut(s) 665, 1284
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 4 cut(s) 130, 287, 816, 818
GluI GCNGC 2 cut(s) 176, 1251
GsuI CTGGAG 1 cut(s) 418
HaeIII GGCC 4 cut(s) 274, 370, 962, 1270
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 176
HhaI GCGC 4 cut(s) 131, 288, 817, 819
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 8 cut(s) 262, 302, 418, 832, 850, 1023, 1028, 1153
Hin6I GCGC 4 cut(s) 129, 286, 815, 817
HinP1I GCGC 4 cut(s) 129, 286, 815, 817
HincII GTYRAC 2 cut(s) 766, 1114
HindII GTYRAC 2 cut(s) 766, 1114
HinfI GANTC 9 cut(s) 146, 223, 431, 659, 850, 913, 1172, 1303, 1313
HpaII CCGG 1 cut(s) 172
HphI GGTGA 2 cut(s) 166, 295
Hpy166II GTNNAC 4 cut(s) 500, 766, 1114, 1144
Hpy188I TCNGA 5 cut(s) 375, 521, 855, 924, 1105
Hpy188III TCNNGA 6 cut(s) 29, 211, 435, 542, 656, 910
Hpy8I GTNNAC 4 cut(s) 500, 766, 1114, 1144
HpyAV CCTTC 4 cut(s) 485, 503, 581, 1273
HpyCH4III ACNGT 4 cut(s) 164, 391, 539, 1091
HpyCH4IV ACGT 2 cut(s) 203, 291
HpyCH4V TGCA 7 cut(s) 380, 418, 472, 685, 839, 875, 1253
HpyF10VI GCNNNNNNNGC 2 cut(s) 682, 721
HpyF3I CTNAG 4 cut(s) 672, 709, 852, 1301
HpySE526I ACGT 2 cut(s) 203, 291
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 8 cut(s) 262, 302, 418, 832, 850, 1023, 1028, 1153
HspAI GCGC 4 cut(s) 129, 286, 815, 817
Ksp22I TGATCA 4 cut(s) 618, 1105, 1192, 1240
Kzo9I GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
LguI GCTCTTC 1 cut(s) 1175
LmnI GCTCC 2 cut(s) 247, 547
Lsp1109I GCAGC 2 cut(s) 187, 1237
MaeI CTAG 2 cut(s) 665, 1284
MaeII ACGT 2 cut(s) 203, 291
MaeIII GTNAC 4 cut(s) 154, 164, 199, 301
MalI GATC 6 cut(s) 266, 620, 864, 1107, 1194, 1242
MboI GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
MfeI CAATTG 2 cut(s) 1155, 1196
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 2 cut(s) 12, 1272
MluCI AATT 8 cut(s) 456, 522, 563, 1071, 1155, 1196, 1204, 1329
MlyI GAGTC 6 cut(s) 155, 232, 425, 844, 1166, 1312
MmeI TCCRAC 1 cut(s) 74
MseI TTAA 4 cut(s) 98, 122, 525, 566
MslI CAYNNNNRTG 2 cut(s) 827, 1029
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 3 cut(s) 172, 228, 1339
MunI CAATTG 2 cut(s) 1155, 1196
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 2 cut(s) 228, 1339
MvnI CGCG 1 cut(s) 817
MwoI GCNNNNNNNGC 2 cut(s) 682, 721
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 2 cut(s) 258, 828
NdeII GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
NlaIII CATG 8 cut(s) 262, 302, 418, 832, 850, 1023, 1028, 1153
NlaIV GGNNCC 1 cut(s) 1270
NmuCI GTSAC 4 cut(s) 154, 164, 199, 301
NsbI TGCGCA 1 cut(s) 130
NspI RCATGY 1 cut(s) 302
PauI GCGCGC 1 cut(s) 815
PceI AGGCCT 1 cut(s) 370
PciI ACATGT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 1175
PctI GAATGC 1 cut(s) 817
PfeI GAWTC 3 cut(s) 659, 913, 1313
PkrI GCNGC 2 cut(s) 177, 1252
PleI GAGTC 6 cut(s) 154, 231, 425, 844, 1166, 1311
PmaCI CACGTG 1 cut(s) 292
PmlI CACGTG 1 cut(s) 292
PpsI GAGTC 6 cut(s) 154, 231, 425, 844, 1166, 1311
Ppu21I YACGTR 1 cut(s) 292
PscI ACATGT 1 cut(s) 298
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 226, 1337
PspCI CACGTG 1 cut(s) 292
PspGI CCWGG 2 cut(s) 226, 1337
PspN4I GGNNCC 1 cut(s) 1270
PspOMI GGGCCC 1 cut(s) 1268
PspPI GGNCC 2 cut(s) 1268, 1269
PstI CTGCAG 1 cut(s) 1255
PstNI CAGNNNCTG 1 cut(s) 1089
PsuI RGATCY 1 cut(s) 862
PteI GCGCGC 1 cut(s) 815
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 2 cut(s) 582, 1099
RsaNI GTAC 2 cut(s) 581, 1098
RseI CAYNNNNRTG 2 cut(s) 827, 1029
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 1175
SaqAI TTAA 4 cut(s) 98, 122, 525, 566
SatI GCNGC 2 cut(s) 176, 1251
Sau3AI GATC 6 cut(s) 264, 618, 862, 1105, 1192, 1240
Sau96I GGNCC 2 cut(s) 1268, 1269
SchI GAGTC 6 cut(s) 155, 232, 425, 844, 1166, 1312
ScrFI CCNGG 3 cut(s) 172, 228, 1339
SduI GDGCHC 2 cut(s) 12, 1272
SfcI CTRYAG 2 cut(s) 500, 1251
SmiMI CAYNNNNRTG 2 cut(s) 827, 1029
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
Sse9I AATT 8 cut(s) 456, 522, 563, 1071, 1155, 1196, 1204, 1329
SseBI AGGCCT 1 cut(s) 370
SsiI CCGC 5 cut(s) 105, 110, 478, 761, 1342
SspMI CTAG 2 cut(s) 665, 1284
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 370
StyD4I CCNGG 3 cut(s) 170, 226, 1337
StyI CCWWGG 2 cut(s) 258, 828
TaaI ACNGT 4 cut(s) 164, 391, 539, 1091
TaiI ACGT 2 cut(s) 206, 294
TaqI TCGA 2 cut(s) 385, 786
TasI AATT 8 cut(s) 456, 522, 563, 1071, 1155, 1196, 1204, 1329
TfiI GAWTC 3 cut(s) 659, 913, 1313
Tru1I TTAA 4 cut(s) 98, 122, 525, 566
Tru9I TTAA 4 cut(s) 98, 122, 525, 566
TscAI CASTG 4 cut(s) 608, 692, 882, 936
TseFI GTSAC 4 cut(s) 154, 164, 199, 301
TseI GCWGC 2 cut(s) 175, 1250
Tsp45I GTSAC 4 cut(s) 154, 164, 199, 301
TspDTI ATGAA 6 cut(s) 33, 824, 914, 1041, 1062, 1143
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 4 cut(s) 608, 692, 882, 936
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 1 cut(s) 310
XceI RCATGY 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 499
XspI CTAG 2 cut(s) 665, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.