RLG00000034370

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010796
Physical Location & Seq
Reverse (-)
41583692 .. 41587801
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034370

Sequence Viewer

Length: 1347 bp
ATGACAGAGCTCGTCCTCCATTCTCATCCTGACCATTTCTCTTCATCTCCAACACCCACCACCTCTTCTTCTTCTTCTTCTTCTTCTTCTCCACCACCCTCCTTAACCTCCGCACCGCCGTCTTCTTTATCCTGCGCAGCCGCGAGGGACGAGTCTTGGGTGACGGACTGTGACGCCCGGGAGCTGTCCCTGTTGGCATTGCTGGTCACGTTTTTCAGGAAGTCTTTGAGTCATTGCAAGAAGGAAAGGAGGGTGCTTTGTGCCATGGAGATCGGCTGGCCTACCAATGTGCGCCACGTGGCACATGTCACCTTTGATAGGTTCAATGGCTTCTTGGGTTTGCCTGTTGAGTTTGAGCCTGAACTTCCCAGTAGGCCTCCGAGTGCAAGTCGAACTGTTTTTGGAGTATCTACCAAATCCATGCAATTGTCTTACGACTCTAGAGGGAATAGTGTGCCGACAATTCTCTTGCTTATGCAAGGGCGGTTGTATGCTGAAGGAGGTCTACAGGTAGAAGGGATATTCAGAATTAACGGAGAAAACAGTCAAGAGGAGCATATAAGAGGCCAATTAAACAGGGGAGATGTACCAGAAGGCATTGACATACACTGCTTGGCAGGGCTGATCAAGGCTTGGTTTAGGGAACTCCCAGTTGGGCTTCTGGATTCTCTAGCCCCTGAGCAGGTAATGCAGTGCCAGACAGAAGATGACTGCTTAGAGCTTGTGAGGCTTCTACCCTCTACCGAAGTTGCTCTGTTGGATTGGGCGGTCAACCTTATGGCTGATGTTGTCGAACACGAACATCTAAACAAGATGAATGCGCGCAACATTGCCATGGTTTTTGCACCGAACATGACTCAGATGGCAGATCCTTTGACTGCACTGGTGTATGCTGTTCAAGTGATGAACTTCCTCAAGATTCTTATTCTGAAGACACTGCGAGAAAGGAAAGAAAATGTGGTAGAGGCCTGTGTCTTATGTGTAGAACCTGCTGATGAGAATGGACACCAGAGCTGTTCGCAATCATGCCGTGAAGATGTTGTCGAAGATAATGAAAAGAGCCTGGTTGAGAACCTAATTCCTAATGGCAGGGACTGTTGTGGTACTTCTGATCAAGTTGACACTTTGGGTAATGAAATGGAAGCTGTAAACACAGTTGAAGTTCAAGGGGACTCTGTGAAGAGCACACCTGATCAATTGAATAATTCAACTTTCAAAATGGGACCTGAAAGAACAAGTGATCGGCAAGCTGCAGTATGGAGAACAGGGCCCGTTGAGAAGGCTAGGAAAATCAGCAACCTGAGTCGCATAGATTCAAGGAGGGAGCTAATTGAAGCCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

449

Amino Acids

49.6

Weight (kDa)

5.12

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 167 - 287 3.3e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 136
Acc36I ACCTGC 2 cut(s) 673, 997
AccI GTMKAC 1 cut(s) 505
AccII CGCG 2 cut(s) 143, 823
AciI CCGC 6 cut(s) 111, 116, 141, 484, 767, 1342
AclWI GGATC 1 cut(s) 863
AcuI CTGAAG 2 cut(s) 516, 950
AcvI CACGTG 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 2 cut(s) 588, 1105
AfiI CCNNNNNNNGG 2 cut(s) 318, 682
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 9 cut(s) 325, 899, 1160, 1166, 1201, 1209, 1216, 1317, 1334
AjnI CCWGG 2 cut(s) 1062, 1337
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 7 cut(s) 10, 184, 721, 1014, 1145, 1250, 1327
AluI AGCT 7 cut(s) 10, 184, 721, 1014, 1145, 1250, 1327
Alw21I GWGCWC 2 cut(s) 12, 1187
AlwI GGATC 1 cut(s) 863
AlwNI CAGNNNCTG 1 cut(s) 1095
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 5 cut(s) 278, 374, 565, 966, 1268
ApaI GGGCCC 1 cut(s) 1272
ApeKI GCWGC 2 cut(s) 137, 1250
AspLEI GCGC 4 cut(s) 137, 294, 823, 825
AspS9I GGNCC 3 cut(s) 1223, 1268, 1269
AsuC2I CCSGG 2 cut(s) 178, 179
AsuHPI GGTGA 2 cut(s) 172, 301
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 1 cut(s) 1223
BaeGI GKGCMC 1 cut(s) 1272
BanII GRGCYC 2 cut(s) 12, 1272
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbrPI CACGTG 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 114, 938
Bbv12I GWGCWC 2 cut(s) 12, 1187
BbvI GCAGC 2 cut(s) 149, 1237
BccI CCATC 1 cut(s) 856
BceAI ACGGC 2 cut(s) 103, 1014
BciT130I CCWGG 2 cut(s) 1064, 1339
BclI TGATCA 3 cut(s) 624, 1111, 1192
BcnI CCSGG 2 cut(s) 178, 179
BfaI CTAG 3 cut(s) 441, 671, 1284
BfmI CTRYAG 2 cut(s) 506, 1251
BfuAI ACCTGC 2 cut(s) 673, 997
BisI GCNGC 3 cut(s) 138, 141, 1251
BlsI GCNGC 3 cut(s) 139, 142, 1252
Bme1390I CCNGG 4 cut(s) 178, 179, 1064, 1339
Bme18I GGWCC 1 cut(s) 1223
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 1223, 1268, 1269
BmiI GGNNCC 2 cut(s) 1224, 1270
BmrFI CCNGG 4 cut(s) 178, 179, 1064, 1339
BmrI ACTGGG 2 cut(s) 363, 644
BmuI ACTGGG 2 cut(s) 363, 644
BpiI GAAGAC 2 cut(s) 114, 938
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 2 cut(s) 178, 179
BsaAI YACGTR 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 177, 264, 834
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 682
Bse1I ACTGG 3 cut(s) 369, 650, 888
Bse3DI GCAATG 3 cut(s) 197, 232, 828
BseBI CCWGG 2 cut(s) 1064, 1339
BseDI CCNNGG 3 cut(s) 177, 264, 834
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 2 cut(s) 318, 682
BseMI GCAATG 3 cut(s) 197, 232, 828
BseMII CTCAG 3 cut(s) 669, 872, 1292
BseNI ACTGG 3 cut(s) 369, 650, 888
BsePI GCGCGC 1 cut(s) 821
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 1272
BseXI GCAGC 2 cut(s) 149, 1237
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 2 cut(s) 143, 823
BshFI GGCC 5 cut(s) 280, 376, 567, 968, 1270
BsiHKAI GWGCWC 2 cut(s) 12, 1187
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 5 cut(s) 161, 172, 1106, 1184, 1236
BslI CCNNNNNNNGG 2 cut(s) 318, 682
BsmFI GGGAC 5 cut(s) 161, 172, 1106, 1184, 1236
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 5 cut(s) 280, 376, 567, 968, 1270
BsoBI CYCGRG 1 cut(s) 177
Bsp120I GGGCCC 1 cut(s) 1268
Bsp1286I GDGCHC 3 cut(s) 12, 1187, 1272
Bsp143I GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
Bsp19I CCATGG 2 cut(s) 264, 834
BspACI CCGC 6 cut(s) 111, 116, 141, 484, 767, 1342
BspANI GGCC 5 cut(s) 280, 376, 567, 968, 1270
BspCNI CTCAG 3 cut(s) 670, 871, 1293
BspFNI CGCG 2 cut(s) 143, 823
BspLI GGNNCC 2 cut(s) 1224, 1270
BspMAI CTGCAG 1 cut(s) 1255
BspMI ACCTGC 2 cut(s) 673, 997
BspPI GGATC 1 cut(s) 863
BspQI GCTCTTC 1 cut(s) 1175
BsrDI GCAATG 3 cut(s) 197, 232, 828
BsrI ACTGG 3 cut(s) 369, 650, 888
BssECI CCNNGG 3 cut(s) 177, 264, 834
BssHII GCGCGC 1 cut(s) 821
BssMI GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 264, 834
Bst2UI CCWGG 2 cut(s) 1064, 1339
Bst4CI ACNGT 5 cut(s) 170, 397, 545, 1097, 1156
Bst6I CTCTTC 3 cut(s) 46, 70, 1175
BstACI GRCGYC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 278, 823, 1248
BstDEI CTNAG 4 cut(s) 678, 715, 858, 1301
BstDSI CCRYGG 2 cut(s) 264, 834
BstENI CCTNNNNNAGG 1 cut(s) 316
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 2 cut(s) 143, 823
BstHHI GCGC 4 cut(s) 137, 294, 823, 825
BstKTI GATC 6 cut(s) 273, 627, 871, 1114, 1195, 1243
BstMBI GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
BstMWI GCNNNNNNNGC 2 cut(s) 688, 727
BstNI CCWGG 2 cut(s) 1064, 1339
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 4 cut(s) 176, 177, 1062, 1337
BstSFI CTRYAG 2 cut(s) 506, 1251
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 2 cut(s) 143, 823
BstV1I GCAGC 2 cut(s) 149, 1237
BstV2I GAAGAC 2 cut(s) 114, 938
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
BsuRI GGCC 5 cut(s) 280, 376, 567, 968, 1270
BtgI CCRYGG 2 cut(s) 264, 834
BtsCI GGATG 1 cut(s) 25
BtsI GCAGTG 3 cut(s) 607, 698, 935
BtsIMutI CAGTG 4 cut(s) 607, 698, 881, 935
BveI ACCTGC 2 cut(s) 673, 997
Cac8I GCNNGC 3 cut(s) 278, 823, 1248
CaiI CAGNNNCTG 1 cut(s) 1095
CfoI GCGC 4 cut(s) 137, 294, 823, 825
Cfr13I GGNCC 3 cut(s) 1223, 1268, 1269
Cfr9I CCCGGG 1 cut(s) 177
CseI GACGC 1 cut(s) 182
Csp6I GTAC 2 cut(s) 587, 1104
CviAII CATG 6 cut(s) 265, 305, 421, 835, 853, 1026
CviQI GTAC 2 cut(s) 587, 1104
DdeI CTNAG 4 cut(s) 678, 715, 858, 1301
DpnI GATC 6 cut(s) 272, 626, 870, 1113, 1194, 1242
DpnII GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
Eam1104I CTCTTC 3 cut(s) 46, 70, 1175
EarI CTCTTC 3 cut(s) 46, 70, 1175
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 264, 834
Eco147I AGGCCT 2 cut(s) 376, 968
Eco24I GRGCYC 2 cut(s) 12, 1272
Eco47I GGWCC 1 cut(s) 1223
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 516, 950
Eco72I CACGTG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoO109I RGGNCCY 2 cut(s) 1223, 1268
EcoRII CCWGG 2 cut(s) 1062, 1337
EcoT14I CCWWGG 2 cut(s) 264, 834
EcoT38I GRGCYC 2 cut(s) 12, 1272
ErhI CCWWGG 2 cut(s) 264, 834
FaeI CATG 6 cut(s) 268, 308, 424, 838, 856, 1029
FaqI GGGAC 5 cut(s) 161, 172, 1106, 1184, 1236
FatI CATG 6 cut(s) 264, 304, 420, 834, 852, 1025
FbaI TGATCA 3 cut(s) 624, 1111, 1192
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 138, 141, 1251
FokI GGATG 1 cut(s) 12
FriOI GRGCYC 2 cut(s) 12, 1272
Fsp4HI GCNGC 3 cut(s) 138, 141, 1251
FspBI CTAG 3 cut(s) 441, 671, 1284
FspI TGCGCA 1 cut(s) 136
GlaI GCGC 4 cut(s) 136, 293, 822, 824
GluI GCNGC 3 cut(s) 138, 141, 1251
HaeIII GGCC 5 cut(s) 280, 376, 567, 968, 1270
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 182
HhaI GCGC 4 cut(s) 137, 294, 823, 825
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 6 cut(s) 268, 308, 424, 838, 856, 1029
Hin6I GCGC 4 cut(s) 135, 292, 821, 823
HinP1I GCGC 4 cut(s) 135, 292, 821, 823
HincII GTYRAC 2 cut(s) 772, 1120
HindII GTYRAC 2 cut(s) 772, 1120
HinfI GANTC 9 cut(s) 152, 229, 437, 665, 856, 919, 1172, 1303, 1313
HpaII CCGG 1 cut(s) 178
HphI GGTGA 2 cut(s) 172, 301
Hpy166II GTNNAC 4 cut(s) 506, 772, 1120, 1150
Hpy188I TCNGA 5 cut(s) 381, 527, 861, 930, 1111
Hpy188III TCNNGA 6 cut(s) 29, 217, 441, 548, 662, 916
Hpy8I GTNNAC 4 cut(s) 506, 772, 1120, 1150
HpyAV CCTTC 5 cut(s) 235, 491, 509, 587, 1273
HpyCH4III ACNGT 5 cut(s) 170, 397, 545, 1097, 1156
HpyCH4IV ACGT 2 cut(s) 209, 297
HpyCH4V TGCA 8 cut(s) 237, 386, 424, 478, 691, 845, 881, 1253
HpyF10VI GCNNNNNNNGC 2 cut(s) 688, 727
HpyF3I CTNAG 4 cut(s) 678, 715, 858, 1301
HpySE526I ACGT 2 cut(s) 209, 297
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 6 cut(s) 268, 308, 424, 838, 856, 1029
HspAI GCGC 4 cut(s) 135, 292, 821, 823
Ksp22I TGATCA 3 cut(s) 624, 1111, 1192
Kzo9I GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
LguI GCTCTTC 1 cut(s) 1175
LmnI GCTCC 3 cut(s) 181, 553, 1324
Lsp1109I GCAGC 2 cut(s) 149, 1237
MaeI CTAG 3 cut(s) 441, 671, 1284
MaeII ACGT 2 cut(s) 209, 297
MaeIII GTNAC 4 cut(s) 160, 170, 205, 307
MalI GATC 6 cut(s) 272, 626, 870, 1113, 1194, 1242
MboI GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
MfeI CAATTG 2 cut(s) 425, 1196
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 3 cut(s) 12, 1187, 1272
MluCI AATT 8 cut(s) 425, 462, 528, 569, 1077, 1196, 1204, 1329
MlyI GAGTC 6 cut(s) 161, 238, 431, 850, 1166, 1312
MmeI TCCRAC 2 cut(s) 74, 738
MseI TTAA 3 cut(s) 104, 531, 572
MslI CAYNNNNRTG 1 cut(s) 833
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 4 cut(s) 178, 179, 1064, 1339
MunI CAATTG 2 cut(s) 425, 1196
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 2 cut(s) 1064, 1339
MvnI CGCG 2 cut(s) 143, 823
MwoI GCNNNNNNNGC 2 cut(s) 688, 727
NciI CCSGG 2 cut(s) 178, 179
NcoI CCATGG 2 cut(s) 264, 834
NdeII GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
NlaIII CATG 6 cut(s) 268, 308, 424, 838, 856, 1029
NlaIV GGNNCC 2 cut(s) 1224, 1270
NmuCI GTSAC 4 cut(s) 160, 170, 205, 307
NsbI TGCGCA 1 cut(s) 136
NspI RCATGY 1 cut(s) 308
PauI GCGCGC 1 cut(s) 821
PceI AGGCCT 2 cut(s) 376, 968
PciI ACATGT 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 1175
PctI GAATGC 1 cut(s) 823
PfeI GAWTC 3 cut(s) 665, 919, 1313
PkrI GCNGC 3 cut(s) 139, 142, 1252
PleI GAGTC 6 cut(s) 160, 237, 431, 850, 1166, 1311
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 6 cut(s) 160, 237, 431, 850, 1166, 1311
Ppu21I YACGTR 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 1223
PscI ACATGT 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 1223
Psp6I CCWGG 2 cut(s) 1062, 1337
PspCI CACGTG 1 cut(s) 298
PspGI CCWGG 2 cut(s) 1062, 1337
PspN4I GGNNCC 2 cut(s) 1224, 1270
PspOMI GGGCCC 1 cut(s) 1268
PspPI GGNCC 3 cut(s) 1223, 1268, 1269
PspPPI RGGWCCY 1 cut(s) 1223
PstI CTGCAG 1 cut(s) 1255
PstNI CAGNNNCTG 1 cut(s) 1095
PsuI RGATCY 1 cut(s) 868
PteI GCGCGC 1 cut(s) 821
RsaI GTAC 2 cut(s) 588, 1105
RsaNI GTAC 2 cut(s) 587, 1104
RseI CAYNNNNRTG 1 cut(s) 833
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 1175
SaqAI TTAA 3 cut(s) 104, 531, 572
SatI GCNGC 3 cut(s) 138, 141, 1251
Sau3AI GATC 6 cut(s) 270, 624, 868, 1111, 1192, 1240
Sau96I GGNCC 3 cut(s) 1223, 1268, 1269
SchI GAGTC 6 cut(s) 161, 238, 431, 850, 1166, 1312
ScrFI CCNGG 4 cut(s) 178, 179, 1064, 1339
SduI GDGCHC 3 cut(s) 12, 1187, 1272
SfcI CTRYAG 2 cut(s) 506, 1251
SinI GGWCC 1 cut(s) 1223
SmaI CCCGGG 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
Sse9I AATT 8 cut(s) 425, 462, 528, 569, 1077, 1196, 1204, 1329
SseBI AGGCCT 2 cut(s) 376, 968
SsiI CCGC 6 cut(s) 111, 116, 141, 484, 767, 1342
SspMI CTAG 3 cut(s) 441, 671, 1284
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 2 cut(s) 376, 968
StyD4I CCNGG 4 cut(s) 176, 177, 1062, 1337
StyI CCWWGG 2 cut(s) 264, 834
TaaI ACNGT 5 cut(s) 170, 397, 545, 1097, 1156
TaiI ACGT 2 cut(s) 212, 300
TaqI TCGA 3 cut(s) 391, 792, 1044
TasI AATT 8 cut(s) 425, 462, 528, 569, 1077, 1196, 1204, 1329
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 3 cut(s) 665, 919, 1313
Tru1I TTAA 3 cut(s) 104, 531, 572
Tru9I TTAA 3 cut(s) 104, 531, 572
TscAI CASTG 4 cut(s) 614, 698, 888, 942
TseFI GTSAC 4 cut(s) 160, 170, 205, 307
TseI GCWGC 2 cut(s) 137, 1250
Tsp45I GTSAC 4 cut(s) 160, 170, 205, 307
TspDTI ATGAA 5 cut(s) 33, 830, 920, 1068, 1149
TspGWI ACGGA 2 cut(s) 179, 549
TspMI CCCGGG 1 cut(s) 177
TspRI CASTG 4 cut(s) 614, 698, 888, 942
VpaK11BI GGWCC 1 cut(s) 1223
XagI CCTNNNNNAGG 1 cut(s) 316
XbaI TCTAGA 1 cut(s) 440
XceI RCATGY 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 3 cut(s) 441, 671, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.