Rroxscaffold_1G00035550

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010796
Physical Location & Seq
Forward (+)
51667587 .. 51672276
4690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035550.1

Sequence Viewer

Length: 1359 bp
ATGACAGAGCTCGTCCTCCGTTCTCATCCTGACCATTTCTCTTCATCTCCAACACCCACCACCACCTCCACCTCTTCTTCTTCTTCTTCTTCTTCTACTCCACCCTCCTTAACCTCCGCACCGCCGTCTTCTTTAACCTGCGCACCCCCGAGGGACGAGTCTTGGGTGACGGACTGTGACGCCCGGCAGCTGTCCCTGTTGGCATTGCTGGTCACGTTTTTCAGGAAGTCTTTGAGTCATTGCAAGAGGGAAAGGAGGGAGCTTTGTGCCATGGAGATCGGCTGGCCTACCAATGTGCGCCACGTGGCACATGTCACCTTTGATAGGTTCAATGGCTTCTTGGGTTTGCCTGTTGAGTTTGAGCCTGAACTTCCCAGTAGGCCTCCGAGTGCAAGCCGAACTGTTTTTGGAGTATCTACCAAATCCATGCAATTGTCTTATGACTCTAGAGGGAATAGTGTGCCGACAATTCTCTTGCTTATGCAAGGGCGGTTGTATGCTGAAGGAGGTCTACAAGTAGAAGGGATATTCAGAATTAATGGAGAAAACAGTCAAGAGGAGCATATAAGAGGCCAATTAAACAGGGGAGATGTACCAGAAGGCATTGACATACACTGCTTGGCGGGGCTGATCAAGGCTTGGTTTAGGGAACTCCCAGTTGGGCTTCTGGATTCTCTAGCCCCTGAGCAGGTAATGCAGTGCCAGACAGAAGATGACTGCTTAGAGCTTGTGAGGCTTCTACCCTCTACCGAAGTTGCTCTATTGGATTGGGCGGTCAACCTTATGGCTGATGTTGTTGAACATGAAGATCTAAACAAGATGAATTCGCGCAACATTGCCATGGTTTTTGCACCGAACATGACTCAGATGGCAGATCCTTTGACTGCACTGATGTATGCTGTTCAAGTGATGAACTTCCTCAAGGTTCTTATTCTGAAGACACTGCGAGAAAGGAAAGAAAATGTGGTAGAGGCCCTTGTCTTTTGTGTAGAACCTGCTGATGAGAATGGACACCAGAGCTGTTCGCAATCATGCCGTGAAGATGTTGTCGAAGATAATGAAAAGAGCCTGGTTGAGAACGTAATTCCTAATGGCAGGGACTGTTGTGGTACTTCTGATCAAGTTGACACTTTGGGTAATGAAATGGAAGCTGTAAACACCATGACAACTGAAGTTCAAGGGGACTCTGTGAAGAGCAAACCTGATCAATTGAATAATTCAACTTTCAAAATGGGACCTGAAAGAACAAGTGATCAGCAAGCTGCAGTATGGGGAACAGTGCCCGTTGAGAAGGCTAGGCAAATCAGCAACCTGACTCGCATAGATTCAAGGAGGGAGCTAACTGAAGCCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

452

Amino Acids

50.17

Weight (kDa)

4.95

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 169 - 289 3.5e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 142
Acc36I ACCTGC 3 cut(s) 146, 679, 1003
AccI GTMKAC 1 cut(s) 511
AccII CGCG 1 cut(s) 829
AciI CCGC 6 cut(s) 117, 122, 490, 623, 773, 1354
AclWI GGATC 1 cut(s) 869
AcsI RAATTY 1 cut(s) 823
AcuI CTGAAG 3 cut(s) 522, 956, 1191
AcvI CACGTG 1 cut(s) 304
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 2 cut(s) 594, 1111
AfiI CCNNNNNNNGG 2 cut(s) 324, 688
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 8 cut(s) 331, 800, 905, 1178, 1213, 1221, 1228, 1329
AjnI CCWGG 2 cut(s) 1068, 1349
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 8 cut(s) 10, 190, 262, 727, 1020, 1151, 1262, 1339
AluI AGCT 8 cut(s) 10, 190, 262, 727, 1020, 1151, 1262, 1339
Alw21I GWGCWC 1 cut(s) 12
AlwI GGATC 1 cut(s) 869
AlwNI CAGNNNCTG 1 cut(s) 1101
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 4 cut(s) 284, 380, 571, 972
ApeKI GCWGC 2 cut(s) 187, 1262
ApoI RAATTY 1 cut(s) 823
AseI ATTAAT 1 cut(s) 537
AspLEI GCGC 3 cut(s) 143, 300, 831
AspS9I GGNCC 2 cut(s) 973, 1235
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 2 cut(s) 178, 307
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 1235
BaeGI GKGCMC 1 cut(s) 1284
BanII GRGCYC 1 cut(s) 12
BarI GAAGNNNNNNTAC 2 cut(s) 495, 527
BbrPI CACGTG 1 cut(s) 304
BbsI GAAGAC 2 cut(s) 120, 944
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 199, 1249
BccI CCATC 1 cut(s) 862
BceAI ACGGC 2 cut(s) 109, 1020
BciT130I CCWGG 2 cut(s) 1070, 1351
BclI TGATCA 4 cut(s) 630, 1117, 1204, 1252
BcnI CCSGG 1 cut(s) 184
BfaI CTAG 3 cut(s) 447, 677, 1296
BfmI CTRYAG 1 cut(s) 1263
BfuAI ACCTGC 3 cut(s) 146, 679, 1003
BglII AGATCT 1 cut(s) 808
BisI GCNGC 2 cut(s) 188, 1263
BlsI GCNGC 2 cut(s) 189, 1264
Bme1390I CCNGG 3 cut(s) 184, 1070, 1351
Bme18I GGWCC 1 cut(s) 1235
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 973, 1235
BmiI GGNNCC 1 cut(s) 1236
BmrFI CCNGG 3 cut(s) 184, 1070, 1351
BmrI ACTGGG 2 cut(s) 369, 650
BmuI ACTGGG 2 cut(s) 369, 650
BpiI GAAGAC 2 cut(s) 120, 944
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 905
BpuMI CCSGG 1 cut(s) 184
BsaAI YACGTR 1 cut(s) 304
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 149, 270, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 688
Bse1I ACTGG 2 cut(s) 375, 656
Bse3DI GCAATG 3 cut(s) 203, 238, 834
BseBI CCWGG 2 cut(s) 1070, 1351
BseDI CCNNGG 3 cut(s) 149, 270, 840
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 2 cut(s) 324, 688
BseMI GCAATG 3 cut(s) 203, 238, 834
BseMII CTCAG 2 cut(s) 675, 878
BseNI ACTGG 2 cut(s) 375, 656
BseRI GAGGAG 1 cut(s) 572
BseSI GKGCMC 1 cut(s) 1284
BseXI GCAGC 2 cut(s) 199, 1249
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 829
BshFI GGCC 4 cut(s) 286, 382, 573, 974
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 5 cut(s) 167, 178, 1112, 1196, 1248
BslI CCNNNNNNNGG 2 cut(s) 324, 688
BsmFI GGGAC 5 cut(s) 167, 178, 1112, 1196, 1248
BsnI GGCC 4 cut(s) 286, 382, 573, 974
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 12, 1284
Bsp143I GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
Bsp19I CCATGG 2 cut(s) 270, 840
BspACI CCGC 6 cut(s) 117, 122, 490, 623, 773, 1354
BspANI GGCC 4 cut(s) 286, 382, 573, 974
BspCNI CTCAG 2 cut(s) 676, 877
BspFNI CGCG 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 1236
BspMAI CTGCAG 1 cut(s) 1267
BspMI ACCTGC 3 cut(s) 146, 679, 1003
BspPI GGATC 1 cut(s) 869
BspQI GCTCTTC 1 cut(s) 1187
BsrDI GCAATG 3 cut(s) 203, 238, 834
BsrI ACTGG 2 cut(s) 375, 656
BssECI CCNNGG 3 cut(s) 149, 270, 840
BssMI GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 270, 840
Bst2UI CCWGG 2 cut(s) 1070, 1351
Bst4CI ACNGT 5 cut(s) 176, 403, 551, 1103, 1279
Bst6I CTCTTC 3 cut(s) 46, 79, 1187
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 694
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 284, 394, 1260
BstDEI CTNAG 3 cut(s) 684, 721, 864
BstDSI CCRYGG 2 cut(s) 270, 840
BstENI CCTNNNNNAGG 1 cut(s) 322
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 829
BstHHI GCGC 3 cut(s) 143, 300, 831
BstKTI GATC 7 cut(s) 279, 633, 811, 877, 1120, 1207, 1255
BstMBI GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
BstMWI GCNNNNNNNGC 2 cut(s) 694, 733
BstNI CCWGG 2 cut(s) 1070, 1351
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 3 cut(s) 182, 1068, 1349
BstSFI CTRYAG 1 cut(s) 1263
BstSLI GKGCMC 1 cut(s) 1284
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 2 cut(s) 199, 1249
BstV2I GAAGAC 2 cut(s) 120, 944
BstX2I RGATCY 2 cut(s) 808, 874
BstYI RGATCY 2 cut(s) 808, 874
BsuRI GGCC 4 cut(s) 286, 382, 573, 974
BtgI CCRYGG 2 cut(s) 270, 840
BtsCI GGATG 1 cut(s) 25
BtsI GCAGTG 3 cut(s) 613, 704, 941
BtsIMutI CAGTG 5 cut(s) 613, 704, 887, 941, 1284
BveI ACCTGC 3 cut(s) 146, 679, 1003
Cac8I GCNNGC 3 cut(s) 284, 394, 1260
CaiI CAGNNNCTG 1 cut(s) 1101
CfoI GCGC 3 cut(s) 143, 300, 831
Cfr13I GGNCC 2 cut(s) 973, 1235
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 593, 1110
CviAII CATG 8 cut(s) 271, 311, 427, 803, 841, 859, 1032, 1162
CviQI GTAC 2 cut(s) 593, 1110
DdeI CTNAG 3 cut(s) 684, 721, 864
DpnI GATC 7 cut(s) 278, 632, 810, 876, 1119, 1206, 1254
DpnII GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
Eam1104I CTCTTC 3 cut(s) 46, 79, 1187
EarI CTCTTC 3 cut(s) 46, 79, 1187
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 270, 840
Eco147I AGGCCT 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 1235
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 3 cut(s) 522, 956, 1191
Eco72I CACGTG 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 973, 1235
EcoRI GAATTC 1 cut(s) 823
EcoRII CCWGG 2 cut(s) 1068, 1349
EcoT14I CCWWGG 2 cut(s) 270, 840
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 270, 840
FaeI CATG 8 cut(s) 274, 314, 430, 806, 844, 862, 1035, 1165
FaqI GGGAC 5 cut(s) 167, 178, 1112, 1196, 1248
FatI CATG 8 cut(s) 270, 310, 426, 802, 840, 858, 1031, 1161
FauI CCCGC 1 cut(s) 616
FbaI TGATCA 4 cut(s) 630, 1117, 1204, 1252
FblI GTMKAC 1 cut(s) 511
Fnu4HI GCNGC 2 cut(s) 188, 1263
FokI GGATG 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 2 cut(s) 188, 1263
FspBI CTAG 3 cut(s) 447, 677, 1296
FspI TGCGCA 1 cut(s) 142
GlaI GCGC 3 cut(s) 142, 299, 830
GluI GCNGC 2 cut(s) 188, 1263
HaeIII GGCC 4 cut(s) 286, 382, 573, 974
HapII CCGG 1 cut(s) 184
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 143, 300, 831
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 8 cut(s) 274, 314, 430, 806, 844, 862, 1035, 1165
Hin6I GCGC 3 cut(s) 141, 298, 829
HinP1I GCGC 3 cut(s) 141, 298, 829
HincII GTYRAC 2 cut(s) 778, 1126
HindII GTYRAC 2 cut(s) 778, 1126
HinfI GANTC 8 cut(s) 158, 235, 443, 671, 862, 1184, 1315, 1325
HpaII CCGG 1 cut(s) 184
HphI GGTGA 2 cut(s) 178, 307
Hpy166II GTNNAC 4 cut(s) 512, 778, 1126, 1156
Hpy188I TCNGA 5 cut(s) 387, 533, 867, 936, 1117
Hpy188III TCNNGA 5 cut(s) 29, 223, 447, 554, 668
Hpy8I GTNNAC 4 cut(s) 512, 778, 1126, 1156
HpyAV CCTTC 4 cut(s) 497, 515, 593, 1285
HpyCH4III ACNGT 5 cut(s) 176, 403, 551, 1103, 1279
HpyCH4IV ACGT 3 cut(s) 215, 303, 1080
HpyCH4V TGCA 8 cut(s) 243, 392, 430, 484, 697, 851, 887, 1265
HpyF10VI GCNNNNNNNGC 2 cut(s) 694, 733
HpyF3I CTNAG 3 cut(s) 684, 721, 864
HpySE526I ACGT 3 cut(s) 215, 303, 1080
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 8 cut(s) 274, 314, 430, 806, 844, 862, 1035, 1165
HspAI GCGC 3 cut(s) 141, 298, 829
Ksp22I TGATCA 4 cut(s) 630, 1117, 1204, 1252
Kzo9I GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
LguI GCTCTTC 1 cut(s) 1187
LmnI GCTCC 3 cut(s) 259, 559, 1336
Lsp1109I GCAGC 2 cut(s) 199, 1249
MaeI CTAG 3 cut(s) 447, 677, 1296
MaeII ACGT 3 cut(s) 215, 303, 1080
MaeIII GTNAC 4 cut(s) 166, 176, 211, 313
MalI GATC 7 cut(s) 278, 632, 810, 876, 1119, 1206, 1254
MboI GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
MfeI CAATTG 2 cut(s) 431, 1208
MflI RGATCY 2 cut(s) 808, 874
MhlI GDGCHC 2 cut(s) 12, 1284
MluCI AATT 8 cut(s) 431, 468, 534, 575, 823, 1083, 1208, 1216
MlyI GAGTC 6 cut(s) 167, 244, 437, 856, 1178, 1309
MmeI TCCRAC 1 cut(s) 74
MseI TTAA 4 cut(s) 110, 134, 537, 578
MslI CAYNNNNRTG 1 cut(s) 839
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 3 cut(s) 184, 1070, 1351
MunI CAATTG 2 cut(s) 431, 1208
MvaI CCWGG 2 cut(s) 1070, 1351
MvnI CGCG 1 cut(s) 829
MwoI GCNNNNNNNGC 2 cut(s) 694, 733
NciI CCSGG 1 cut(s) 184
NcoI CCATGG 2 cut(s) 270, 840
NdeII GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
NlaIII CATG 8 cut(s) 274, 314, 430, 806, 844, 862, 1035, 1165
NlaIV GGNNCC 1 cut(s) 1236
NmuCI GTSAC 4 cut(s) 166, 176, 211, 313
NsbI TGCGCA 1 cut(s) 142
NspI RCATGY 1 cut(s) 314
PceI AGGCCT 1 cut(s) 382
PciI ACATGT 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 1187
PfeI GAWTC 2 cut(s) 671, 1325
PkrI GCNGC 2 cut(s) 189, 1264
PleI GAGTC 6 cut(s) 166, 243, 437, 856, 1178, 1309
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 6 cut(s) 166, 243, 437, 856, 1178, 1309
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 1235
PscI ACATGT 1 cut(s) 310
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 1235
Psp6I CCWGG 2 cut(s) 1068, 1349
PspCI CACGTG 1 cut(s) 304
PspGI CCWGG 2 cut(s) 1068, 1349
PspN4I GGNNCC 1 cut(s) 1236
PspPI GGNCC 2 cut(s) 973, 1235
PspPPI RGGWCCY 1 cut(s) 1235
PstI CTGCAG 1 cut(s) 1267
PstNI CAGNNNCTG 1 cut(s) 1101
PsuI RGATCY 2 cut(s) 808, 874
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 2 cut(s) 594, 1111
RsaNI GTAC 2 cut(s) 593, 1110
RseI CAYNNNNRTG 1 cut(s) 839
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 1187
SaqAI TTAA 4 cut(s) 110, 134, 537, 578
SatI GCNGC 2 cut(s) 188, 1263
Sau3AI GATC 7 cut(s) 276, 630, 808, 874, 1117, 1204, 1252
Sau96I GGNCC 2 cut(s) 973, 1235
SchI GAGTC 6 cut(s) 167, 244, 437, 856, 1178, 1309
ScrFI CCNGG 3 cut(s) 184, 1070, 1351
SduI GDGCHC 2 cut(s) 12, 1284
SfcI CTRYAG 1 cut(s) 1263
SinI GGWCC 1 cut(s) 1235
SmiMI CAYNNNNRTG 1 cut(s) 839
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 8 cut(s) 431, 468, 534, 575, 823, 1083, 1208, 1216
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 6 cut(s) 117, 122, 490, 623, 773, 1354
SspMI CTAG 3 cut(s) 447, 677, 1296
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 3 cut(s) 182, 1068, 1349
StyI CCWWGG 2 cut(s) 270, 840
TaaI ACNGT 5 cut(s) 176, 403, 551, 1103, 1279
TaiI ACGT 3 cut(s) 218, 306, 1083
TaqI TCGA 1 cut(s) 1050
TasI AATT 8 cut(s) 431, 468, 534, 575, 823, 1083, 1208, 1216
TfiI GAWTC 2 cut(s) 671, 1325
Tru1I TTAA 4 cut(s) 110, 134, 537, 578
Tru9I TTAA 4 cut(s) 110, 134, 537, 578
TscAI CASTG 5 cut(s) 620, 704, 894, 948, 1284
TseFI GTSAC 4 cut(s) 166, 176, 211, 313
TseI GCWGC 2 cut(s) 187, 1262
Tsp45I GTSAC 4 cut(s) 166, 176, 211, 313
TspDTI ATGAA 6 cut(s) 33, 819, 836, 926, 1074, 1155
TspGWI ACGGA 2 cut(s) 8, 185
TspRI CASTG 5 cut(s) 620, 704, 894, 948, 1284
VpaK11BI GGWCC 1 cut(s) 1235
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 322
XapI RAATTY 1 cut(s) 823
XbaI TCTAGA 1 cut(s) 446
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 511
XspI CTAG 3 cut(s) 447, 677, 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.