RchiOBHm_Chr5g0045671

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010796
Physical Location & Seq
Reverse (-)
41685343 .. 41690006
4664 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32373

Sequence Viewer

Length: 1341 bp
ATGACAGAGCTCGTCCTCCATTCTCATCCTGACCATTTCTCTACATCTCCAACACCCACCACCTCTTCTTCTTCTTCTTCTTCTCCACCACCCTCCTTAACCTCCGCACCGCCGTCTTCTTTAACCTGCGCACCCCCGAGGGATGAGTCTTGGGTGACGGACTGTGACGCCCGGCAGCAGCTGTCCCTGTTGGCATTGCTGGTCACGTTTTTCAGGAAGTCTTTGAGTCCTTGCAAGAGGGAAAGGAGGGAGCTTTGCGCCATGGAGATCGGCTGGCCTACCAATGTGCGCCACGTGGCACATGTCACCTTTGACAGGTTCAATGGCTTCTTGGGTTTGCCTGTTGAGTTTGAGCCTGAACTTCCCAGTAGGCCTCCGAGTGCAAGTCGAACTGTTTTTGGAGTATCTACCAAATCCATGCAATTGTCTTATGACTCTAGAGGGAATAGTGTGCCGACAATTCTCTTGCTTATGCAAGGGCGGTTGTATGCTGAAGGAGGTCTACAGGTAGAAGGGATATTCAGAATTAATGGAGAAAACAGTCAAGAGGAGCATATAAGAGGCCAGTTAAACAGGGGAGATGTACCTGAAGGCATTGACATACACTGCTTGGCAGGGCTGATCAAGGCTTGGTTTAGGGAACTCCCAGTTGGGCTTCTGGATTCTCTAGCCCCTGAGCAGGTAATGCAGTGCCAGACAGAAGACGACTGCTTAGAGCTTGTGAGGCTTCTACCCTCTACCGAAGTTGCTCTATTGGATTGGGCGGTCAACCTTATGGCTGATGTTGTCAAACACGAACATCTAAACAAGATGAATGCGCGCAATATTGCCATGGTTTTTGCACCGAACATGACTCAGATGGCAGATCCTTTGACTGCACTGATGTATGCTGTTCAAGTGATGAACTTCCTCAAGATTCTTATTCTGAAGACACTGCAAGAAAGGAAAGAAAATGTGGTAGAGGCCCGTGTCTTTTGTGTAGAACCTGCTGATGAGAATGGACACCAGAGCTGTTTGCAATCATGCCATGAAGATGTTGTCAAAGAGAGCCTCGTTGAGAACCCAATTCCTAATGGCAGGGACTGTTGTGGTACTTCTGATCAAGTTGACACTTTGGGTAATGAAATGGAAGCTGTAAACACCATGACAACTGAAGTTCAAGGGGACTCTGTGAAGAGCAAACCTGATCAATTGAATAATTCAACTTTCAAAATGGGACCTGAAAGAATAAGTGATCAGCAAGCTGCAGTATGGGGAACAGGGCCCGTTGAGAAGGCTAGGAAAATCAGCAACCTGAATCGCATAGATTCAAGGAGGGAGCTAATTGAAGCCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

446

Amino Acids

49.55

Weight (kDa)

5.21

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 166 - 286 2.7e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 3 cut(s) 134, 670, 994
AccI GTMKAC 1 cut(s) 502
AccII CGCG 1 cut(s) 820
AciI CCGC 5 cut(s) 105, 110, 481, 764, 1336
AclWI GGATC 1 cut(s) 860
AcuI CTGAAG 4 cut(s) 513, 609, 947, 1173
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 585, 1093
AfiI CCNNNNNNNGG 2 cut(s) 315, 679
AflIII ACRYGT 1 cut(s) 301
AgsI TTSAA 8 cut(s) 322, 896, 1160, 1195, 1203, 1210, 1311, 1328
AjnI CCWGG 1 cut(s) 1331
AjuI GAANNNNNNNTTGG 2 cut(s) 633, 665
AluBI AGCT 8 cut(s) 10, 181, 253, 718, 1011, 1133, 1244, 1321
AluI AGCT 8 cut(s) 10, 181, 253, 718, 1011, 1133, 1244, 1321
Alw21I GWGCWC 1 cut(s) 12
AlwI GGATC 1 cut(s) 860
AlwNI CAGNNNCTG 2 cut(s) 181, 1083
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 5 cut(s) 275, 371, 562, 963, 1262
ApaI GGGCCC 1 cut(s) 1266
ApeKI GCWGC 3 cut(s) 175, 178, 1244
AseI ATTAAT 1 cut(s) 528
AspLEI GCGC 5 cut(s) 131, 260, 291, 820, 822
AspS9I GGNCC 4 cut(s) 964, 1217, 1262, 1263
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 166, 298
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 1 cut(s) 1217
BaeGI GKGCMC 1 cut(s) 1266
BanII GRGCYC 2 cut(s) 12, 1266
BarI GAAGNNNNNNTAC 2 cut(s) 486, 518
BbrPI CACGTG 1 cut(s) 295
BbsI GAAGAC 3 cut(s) 108, 708, 935
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 187, 190, 1231
BccI CCATC 1 cut(s) 853
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 1333
BclI TGATCA 4 cut(s) 621, 1099, 1186, 1234
BcnI CCSGG 1 cut(s) 172
BfaI CTAG 3 cut(s) 438, 668, 1278
BfmI CTRYAG 2 cut(s) 503, 1245
BfuAI ACCTGC 3 cut(s) 134, 670, 994
BisI GCNGC 3 cut(s) 176, 179, 1245
BlsI GCNGC 3 cut(s) 177, 180, 1246
Bme1390I CCNGG 2 cut(s) 172, 1333
Bme18I GGWCC 1 cut(s) 1217
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 4 cut(s) 964, 1217, 1262, 1263
BmiI GGNNCC 2 cut(s) 1218, 1264
BmrFI CCNGG 2 cut(s) 172, 1333
BmrI ACTGGG 2 cut(s) 360, 641
BmuI ACTGGG 2 cut(s) 360, 641
BpiI GAAGAC 3 cut(s) 108, 708, 935
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 896
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 137, 261, 831
Bsc4I CCNNNNNNNGG 2 cut(s) 315, 679
Bse1I ACTGG 3 cut(s) 366, 565, 647
Bse3DI GCAATG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 1333
BseDI CCNNGG 3 cut(s) 137, 261, 831
BseGI GGATG 2 cut(s) 25, 148
BseLI CCNNNNNNNGG 2 cut(s) 315, 679
BseMI GCAATG 1 cut(s) 194
BseMII CTCAG 2 cut(s) 666, 869
BseNI ACTGG 3 cut(s) 366, 565, 647
BsePI GCGCGC 1 cut(s) 818
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 1266
BseXI GCAGC 3 cut(s) 187, 190, 1231
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 820
BshFI GGCC 5 cut(s) 277, 373, 564, 965, 1264
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 172
BslFI GGGAC 4 cut(s) 169, 1094, 1178, 1230
BslI CCNNNNNNNGG 2 cut(s) 315, 679
BsmFI GGGAC 4 cut(s) 169, 1094, 1178, 1230
BsmI GAATGC 1 cut(s) 820
BsnI GGCC 5 cut(s) 277, 373, 564, 965, 1264
BsoBI CYCGRG 1 cut(s) 136
Bsp120I GGGCCC 1 cut(s) 1262
Bsp1286I GDGCHC 2 cut(s) 12, 1266
Bsp143I GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
Bsp19I CCATGG 2 cut(s) 261, 831
BspACI CCGC 5 cut(s) 105, 110, 481, 764, 1336
BspANI GGCC 5 cut(s) 277, 373, 564, 965, 1264
BspCNI CTCAG 2 cut(s) 667, 868
BspFNI CGCG 1 cut(s) 820
BspLI GGNNCC 2 cut(s) 1218, 1264
BspMAI CTGCAG 1 cut(s) 1249
BspMI ACCTGC 3 cut(s) 134, 670, 994
BspPI GGATC 1 cut(s) 860
BspQI GCTCTTC 1 cut(s) 1169
BsrDI GCAATG 1 cut(s) 194
BsrI ACTGG 3 cut(s) 366, 565, 647
BssECI CCNNGG 3 cut(s) 137, 261, 831
BssHII GCGCGC 1 cut(s) 818
BssMI GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 2 cut(s) 261, 831
Bst2UI CCWGG 1 cut(s) 1333
Bst4CI ACNGT 4 cut(s) 164, 394, 542, 1085
Bst6I CTCTTC 2 cut(s) 70, 1169
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 685
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 3 cut(s) 275, 820, 1242
BstDEI CTNAG 3 cut(s) 675, 712, 855
BstDSI CCRYGG 2 cut(s) 261, 831
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 25, 148
BstFNI CGCG 1 cut(s) 820
BstHHI GCGC 5 cut(s) 131, 260, 291, 820, 822
BstKTI GATC 6 cut(s) 270, 624, 868, 1102, 1189, 1237
BstMBI GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
BstMWI GCNNNNNNNGC 2 cut(s) 685, 724
BstNI CCWGG 1 cut(s) 1333
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 2 cut(s) 170, 1331
BstSFI CTRYAG 2 cut(s) 503, 1245
BstSLI GKGCMC 1 cut(s) 1266
BstUI CGCG 1 cut(s) 820
BstV1I GCAGC 3 cut(s) 187, 190, 1231
BstV2I GAAGAC 3 cut(s) 108, 708, 935
BstX2I RGATCY 1 cut(s) 865
BstYI RGATCY 1 cut(s) 865
BsuRI GGCC 5 cut(s) 277, 373, 564, 965, 1264
BtgI CCRYGG 2 cut(s) 261, 831
BtsCI GGATG 2 cut(s) 25, 148
BtsI GCAGTG 3 cut(s) 604, 695, 932
BtsIMutI CAGTG 4 cut(s) 604, 695, 878, 932
BveI ACCTGC 3 cut(s) 134, 670, 994
Cac8I GCNNGC 3 cut(s) 275, 820, 1242
CaiI CAGNNNCTG 2 cut(s) 181, 1083
CfoI GCGC 5 cut(s) 131, 260, 291, 820, 822
Cfr13I GGNCC 4 cut(s) 964, 1217, 1262, 1263
CseI GACGC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 584, 1092
CviAII CATG 8 cut(s) 262, 302, 418, 832, 850, 1023, 1028, 1144
CviQI GTAC 2 cut(s) 584, 1092
DdeI CTNAG 3 cut(s) 675, 712, 855
DpnI GATC 6 cut(s) 269, 623, 867, 1101, 1188, 1236
DpnII GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
Eam1104I CTCTTC 2 cut(s) 70, 1169
EarI CTCTTC 2 cut(s) 70, 1169
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 261, 831
Eco147I AGGCCT 1 cut(s) 373
Eco24I GRGCYC 2 cut(s) 12, 1266
Eco47I GGWCC 1 cut(s) 1217
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 4 cut(s) 513, 609, 947, 1173
Eco72I CACGTG 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoO109I RGGNCCY 2 cut(s) 1217, 1262
EcoRII CCWGG 1 cut(s) 1331
EcoT14I CCWWGG 2 cut(s) 261, 831
EcoT38I GRGCYC 2 cut(s) 12, 1266
ErhI CCWWGG 2 cut(s) 261, 831
FaeI CATG 8 cut(s) 265, 305, 421, 835, 853, 1026, 1031, 1147
FaqI GGGAC 4 cut(s) 169, 1094, 1178, 1230
FatI CATG 8 cut(s) 261, 301, 417, 831, 849, 1022, 1027, 1143
FbaI TGATCA 4 cut(s) 621, 1099, 1186, 1234
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 3 cut(s) 176, 179, 1245
FokI GGATG 2 cut(s) 12, 155
FriOI GRGCYC 2 cut(s) 12, 1266
Fsp4HI GCNGC 3 cut(s) 176, 179, 1245
FspBI CTAG 3 cut(s) 438, 668, 1278
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 5 cut(s) 130, 259, 290, 819, 821
GluI GCNGC 3 cut(s) 176, 179, 1245
HaeIII GGCC 5 cut(s) 277, 373, 564, 965, 1264
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 176
HhaI GCGC 5 cut(s) 131, 260, 291, 820, 822
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 8 cut(s) 265, 305, 421, 835, 853, 1026, 1031, 1147
Hin6I GCGC 5 cut(s) 129, 258, 289, 818, 820
HinP1I GCGC 5 cut(s) 129, 258, 289, 818, 820
HincII GTYRAC 2 cut(s) 769, 1108
HindII GTYRAC 2 cut(s) 769, 1108
HinfI GANTC 9 cut(s) 146, 226, 434, 662, 853, 916, 1166, 1297, 1307
HpaII CCGG 1 cut(s) 172
HphI GGTGA 2 cut(s) 166, 298
Hpy166II GTNNAC 4 cut(s) 503, 769, 1108, 1138
Hpy188I TCNGA 5 cut(s) 378, 524, 858, 927, 1099
Hpy188III TCNNGA 6 cut(s) 29, 214, 438, 545, 659, 913
Hpy8I GTNNAC 4 cut(s) 503, 769, 1108, 1138
HpyAV CCTTC 4 cut(s) 488, 506, 584, 1267
HpyCH4III ACNGT 4 cut(s) 164, 394, 542, 1085
HpyCH4IV ACGT 2 cut(s) 206, 294
HpyF10VI GCNNNNNNNGC 2 cut(s) 685, 724
HpyF3I CTNAG 3 cut(s) 675, 712, 855
HpySE526I ACGT 2 cut(s) 206, 294
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 8 cut(s) 265, 305, 421, 835, 853, 1026, 1031, 1147
HspAI GCGC 5 cut(s) 129, 258, 289, 818, 820
Ksp22I TGATCA 4 cut(s) 621, 1099, 1186, 1234
Kzo9I GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
LguI GCTCTTC 1 cut(s) 1169
LmnI GCTCC 3 cut(s) 250, 550, 1318
Lsp1109I GCAGC 3 cut(s) 187, 190, 1231
MaeI CTAG 3 cut(s) 438, 668, 1278
MaeII ACGT 2 cut(s) 206, 294
MaeIII GTNAC 4 cut(s) 154, 164, 202, 304
MalI GATC 6 cut(s) 269, 623, 867, 1101, 1188, 1236
MboI GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
MfeI CAATTG 2 cut(s) 422, 1190
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 2 cut(s) 12, 1266
MluCI AATT 7 cut(s) 422, 459, 525, 1065, 1190, 1198, 1323
MlyI GAGTC 5 cut(s) 155, 235, 428, 847, 1160
MmeI TCCRAC 1 cut(s) 74
MseI TTAA 4 cut(s) 98, 122, 528, 569
MslI CAYNNNNRTG 1 cut(s) 1032
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 2 cut(s) 172, 1333
MunI CAATTG 2 cut(s) 422, 1190
Mva1269I GAATGC 1 cut(s) 820
MvaI CCWGG 1 cut(s) 1333
MvnI CGCG 1 cut(s) 820
MwoI GCNNNNNNNGC 2 cut(s) 685, 724
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 2 cut(s) 261, 831
NdeII GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
NlaIII CATG 8 cut(s) 265, 305, 421, 835, 853, 1026, 1031, 1147
NlaIV GGNNCC 2 cut(s) 1218, 1264
NmuCI GTSAC 4 cut(s) 154, 164, 202, 304
NsbI TGCGCA 1 cut(s) 130
NspI RCATGY 1 cut(s) 305
PauI GCGCGC 1 cut(s) 818
PceI AGGCCT 1 cut(s) 373
PciI ACATGT 1 cut(s) 301
PciSI GCTCTTC 1 cut(s) 1169
PctI GAATGC 1 cut(s) 820
PfeI GAWTC 4 cut(s) 662, 916, 1297, 1307
PkrI GCNGC 3 cut(s) 177, 180, 1246
PleI GAGTC 5 cut(s) 154, 234, 428, 847, 1160
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
PpsI GAGTC 5 cut(s) 154, 234, 428, 847, 1160
Ppu21I YACGTR 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 1217
PscI ACATGT 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 1217
Psp6I CCWGG 1 cut(s) 1331
PspCI CACGTG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 1331
PspN4I GGNNCC 2 cut(s) 1218, 1264
PspOMI GGGCCC 1 cut(s) 1262
PspPI GGNCC 4 cut(s) 964, 1217, 1262, 1263
PspPPI RGGWCCY 1 cut(s) 1217
PstI CTGCAG 1 cut(s) 1249
PstNI CAGNNNCTG 2 cut(s) 181, 1083
PsuI RGATCY 1 cut(s) 865
PteI GCGCGC 1 cut(s) 818
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 2 cut(s) 585, 1093
RsaNI GTAC 2 cut(s) 584, 1092
RseI CAYNNNNRTG 1 cut(s) 1032
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 1169
SaqAI TTAA 4 cut(s) 98, 122, 528, 569
SatI GCNGC 3 cut(s) 176, 179, 1245
Sau3AI GATC 6 cut(s) 267, 621, 865, 1099, 1186, 1234
Sau96I GGNCC 4 cut(s) 964, 1217, 1262, 1263
SchI GAGTC 5 cut(s) 155, 235, 428, 847, 1160
ScrFI CCNGG 2 cut(s) 172, 1333
SduI GDGCHC 2 cut(s) 12, 1266
SfcI CTRYAG 2 cut(s) 503, 1245
SinI GGWCC 1 cut(s) 1217
SmiMI CAYNNNNRTG 1 cut(s) 1032
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 7 cut(s) 422, 459, 525, 1065, 1190, 1198, 1323
SseBI AGGCCT 1 cut(s) 373
SsiI CCGC 5 cut(s) 105, 110, 481, 764, 1336
SspI AATATT 1 cut(s) 826
SspMI CTAG 3 cut(s) 438, 668, 1278
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 373
StyD4I CCNGG 2 cut(s) 170, 1331
StyI CCWWGG 2 cut(s) 261, 831
TaaI ACNGT 4 cut(s) 164, 394, 542, 1085
TaiI ACGT 2 cut(s) 209, 297
TaqI TCGA 1 cut(s) 388
TasI AATT 7 cut(s) 422, 459, 525, 1065, 1190, 1198, 1323
TfiI GAWTC 4 cut(s) 662, 916, 1297, 1307
Tru1I TTAA 4 cut(s) 98, 122, 528, 569
Tru9I TTAA 4 cut(s) 98, 122, 528, 569
TscAI CASTG 4 cut(s) 611, 695, 885, 939
TseFI GTSAC 4 cut(s) 154, 164, 202, 304
TseI GCWGC 3 cut(s) 175, 178, 1244
Tsp45I GTSAC 4 cut(s) 154, 164, 202, 304
TspDTI ATGAA 4 cut(s) 827, 917, 1044, 1137
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 4 cut(s) 611, 695, 885, 939
VpaK11BI GGWCC 1 cut(s) 1217
VspI ATTAAT 1 cut(s) 528
XagI CCTNNNNNAGG 1 cut(s) 313
XbaI TCTAGA 1 cut(s) 437
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 3 cut(s) 438, 668, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.