RchiOBHm_Chr5g0046331

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010796
Physical Location & Seq
Forward (+)
42367857 .. 42371929
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32433

Sequence Viewer

Length: 1080 bp
ATGGAGATCGGCTGGCCTACCAATGTGCGCCACGTGGCACATGTCACCTTTGACAGGTTCAATGGCTTCTTGGGTTTGCCTGTTGAGTTTGAGCCTGAACTTCCCAGTAGGCCTCCGAGTGCAAGTCGAACTGTTTTTGGAGTATCTACCAAATCCATGCAATTGTCTTATGACTCTAGAGGGAATAGTGTGCCGACAATTCTCTTGCTTATGCAAGGGCGGTTGTATGCTGAAGGAGGTCTACAGGTAGAAGGGATATTCAGAATTAATGGAGAAAACAGTCAAGAGGAGCATATAAGAGGCCAGTTAAACAGGGGAGATGTACCTGAAGGCATTGACATACACTGCTTGGCAGGGCTGATCAAGGCTTGGTTTAGGGAACTCCCAGTTGGGCTTCTGGATTCTCTAGCCCCTGAGCAGGTAATGCAGTGCCAGACAGAAGACGACTGCTTAGAGCTTGTGAGGCTTCTACCCTCTACCGAAGTTGCTCTATTGGATTGGGCGGTCAACCTTATGGCTGATGTTGTCAAACACGAACATCTAAACAAGATGAATGCGCGCAATATTGCCATGGTTTTTGCACCGAACATGACTCAGATGGCAGATCCTTTGACTGCACTGATGTATGCTGTTCAAGTGATGAACTTCCTCAAGATTCTTATTCTGAAGACACTGCAAGAAAGGAAAGAAAATGTGGTAGAGGCCCGTGTCTTTTGTGTAGAACCTGCTGATGAGAATGGACACCAGAGCTGTTTGCAATCATGCCATGAAGATGTTGTCAAAGAGAGCCTCGTTGAGAACCCAATTCCTAATGGCAGGGACTGTTGTGGTACTTCTGATCAAGTTGACACTTTGGGTAATGAAATGGAAGCTGTAAACACCATGACAACTGAAGTTCAAGGGGACTCTGTGAAGAGCAAACCTGATCAATTGAATAATTCAACTTTCAAAATGGGACCTGAAAGAATAAGTGATCAGCAAGCTGCAGTATGGGGAACAGGGCCCGTTGAGAAGGCTAGGAAAATCAGCAACCTGAATCGCATAGATTCAAGGAGGGAGCTAATTGAAGCCTGGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

359

Amino Acids

40.09

Weight (kDa)

5.0

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 79 - 199 1.7e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 409, 733
AccI GTMKAC 1 cut(s) 241
AccII CGCG 1 cut(s) 559
AciI CCGC 3 cut(s) 220, 503, 1075
AclWI GGATC 1 cut(s) 599
AcuI CTGAAG 4 cut(s) 252, 348, 686, 912
AcvI CACGTG 1 cut(s) 34
AfaI GTAC 2 cut(s) 324, 832
AfiI CCNNNNNNNGG 2 cut(s) 54, 418
AflIII ACRYGT 1 cut(s) 40
AgsI TTSAA 8 cut(s) 61, 635, 899, 934, 942, 949, 1050, 1067
AjnI CCWGG 1 cut(s) 1070
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 5 cut(s) 457, 750, 872, 983, 1060
AluI AGCT 5 cut(s) 457, 750, 872, 983, 1060
AlwI GGATC 1 cut(s) 599
AlwNI CAGNNNCTG 1 cut(s) 822
AoxI GGCC 5 cut(s) 14, 110, 301, 702, 1001
ApaI GGGCCC 1 cut(s) 1005
ApeKI GCWGC 1 cut(s) 983
AseI ATTAAT 1 cut(s) 267
AspLEI GCGC 3 cut(s) 30, 559, 561
AspS9I GGNCC 4 cut(s) 703, 956, 1001, 1002
AsuHPI GGTGA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 956
BaeGI GKGCMC 1 cut(s) 1005
BanII GRGCYC 1 cut(s) 1005
BarI GAAGNNNNNNTAC 2 cut(s) 225, 257
BbrPI CACGTG 1 cut(s) 34
BbsI GAAGAC 2 cut(s) 447, 674
BbvI GCAGC 1 cut(s) 970
BccI CCATC 1 cut(s) 592
BciT130I CCWGG 1 cut(s) 1072
BclI TGATCA 4 cut(s) 360, 838, 925, 973
BfaI CTAG 3 cut(s) 177, 407, 1017
BfmI CTRYAG 2 cut(s) 242, 984
BfuAI ACCTGC 2 cut(s) 409, 733
BisI GCNGC 1 cut(s) 984
BlsI GCNGC 1 cut(s) 985
Bme1390I CCNGG 1 cut(s) 1072
Bme18I GGWCC 1 cut(s) 956
BmgT120I GGNCC 4 cut(s) 703, 956, 1001, 1002
BmiI GGNNCC 2 cut(s) 957, 1003
BmrFI CCNGG 1 cut(s) 1072
BmrI ACTGGG 2 cut(s) 99, 380
BmuI ACTGGG 2 cut(s) 99, 380
BpiI GAAGAC 2 cut(s) 447, 674
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 570
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 418
Bse1I ACTGG 3 cut(s) 105, 304, 386
BseBI CCWGG 1 cut(s) 1072
BseDI CCNNGG 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 54, 418
BseMII CTCAG 2 cut(s) 405, 608
BseNI ACTGG 3 cut(s) 105, 304, 386
BsePI GCGCGC 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 1005
BseXI GCAGC 1 cut(s) 970
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 559
BshFI GGCC 5 cut(s) 16, 112, 303, 704, 1003
BslFI GGGAC 3 cut(s) 833, 917, 969
BslI CCNNNNNNNGG 2 cut(s) 54, 418
BsmFI GGGAC 3 cut(s) 833, 917, 969
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 5 cut(s) 16, 112, 303, 704, 1003
Bsp120I GGGCCC 1 cut(s) 1001
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp143I GATC 6 cut(s) 6, 360, 604, 838, 925, 973
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 3 cut(s) 220, 503, 1075
BspANI GGCC 5 cut(s) 16, 112, 303, 704, 1003
BspCNI CTCAG 2 cut(s) 406, 607
BspFNI CGCG 1 cut(s) 559
BspLI GGNNCC 2 cut(s) 957, 1003
BspMAI CTGCAG 1 cut(s) 988
BspMI ACCTGC 2 cut(s) 409, 733
BspPI GGATC 1 cut(s) 599
BspQI GCTCTTC 1 cut(s) 908
BsrI ACTGG 3 cut(s) 105, 304, 386
BssECI CCNNGG 1 cut(s) 570
BssHII GCGCGC 1 cut(s) 557
BssMI GATC 6 cut(s) 6, 360, 604, 838, 925, 973
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 1072
Bst4CI ACNGT 3 cut(s) 133, 281, 824
Bst6I CTCTTC 1 cut(s) 908
BstAPI GCANNNNNTGC 1 cut(s) 424
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 3 cut(s) 14, 559, 981
BstDEI CTNAG 3 cut(s) 414, 451, 594
BstDSI CCRYGG 1 cut(s) 570
BstENI CCTNNNNNAGG 1 cut(s) 52
BstFNI CGCG 1 cut(s) 559
BstHHI GCGC 3 cut(s) 30, 559, 561
BstKTI GATC 6 cut(s) 9, 363, 607, 841, 928, 976
BstMBI GATC 6 cut(s) 6, 360, 604, 838, 925, 973
BstMWI GCNNNNNNNGC 2 cut(s) 424, 463
BstNI CCWGG 1 cut(s) 1072
BstNSI RCATGY 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 1070
BstSFI CTRYAG 2 cut(s) 242, 984
BstSLI GKGCMC 1 cut(s) 1005
BstUI CGCG 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 970
BstV2I GAAGAC 2 cut(s) 447, 674
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 5 cut(s) 16, 112, 303, 704, 1003
BtgI CCRYGG 1 cut(s) 570
BtsI GCAGTG 3 cut(s) 343, 434, 671
BtsIMutI CAGTG 4 cut(s) 343, 434, 617, 671
BveI ACCTGC 2 cut(s) 409, 733
Cac8I GCNNGC 3 cut(s) 14, 559, 981
CaiI CAGNNNCTG 1 cut(s) 822
CfoI GCGC 3 cut(s) 30, 559, 561
Cfr13I GGNCC 4 cut(s) 703, 956, 1001, 1002
Csp6I GTAC 2 cut(s) 323, 831
CviAII CATG 7 cut(s) 41, 157, 571, 589, 762, 767, 883
CviQI GTAC 2 cut(s) 323, 831
DdeI CTNAG 3 cut(s) 414, 451, 594
DpnI GATC 6 cut(s) 8, 362, 606, 840, 927, 975
DpnII GATC 6 cut(s) 6, 360, 604, 838, 925, 973
Eam1104I CTCTTC 1 cut(s) 908
EarI CTCTTC 1 cut(s) 908
Eco130I CCWWGG 1 cut(s) 570
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 1005
Eco47I GGWCC 1 cut(s) 956
Eco57I CTGAAG 4 cut(s) 252, 348, 686, 912
Eco72I CACGTG 1 cut(s) 34
EcoNI CCTNNNNNAGG 1 cut(s) 52
EcoO109I RGGNCCY 2 cut(s) 956, 1001
EcoRII CCWGG 1 cut(s) 1070
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 1005
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 7 cut(s) 44, 160, 574, 592, 765, 770, 886
FaqI GGGAC 3 cut(s) 833, 917, 969
FatI CATG 7 cut(s) 40, 156, 570, 588, 761, 766, 882
FbaI TGATCA 4 cut(s) 360, 838, 925, 973
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 984
FriOI GRGCYC 1 cut(s) 1005
Fsp4HI GCNGC 1 cut(s) 984
FspBI CTAG 3 cut(s) 177, 407, 1017
GlaI GCGC 3 cut(s) 29, 558, 560
GluI GCNGC 1 cut(s) 984
HaeIII GGCC 5 cut(s) 16, 112, 303, 704, 1003
HhaI GCGC 3 cut(s) 30, 559, 561
Hin1II CATG 7 cut(s) 44, 160, 574, 592, 765, 770, 886
Hin6I GCGC 3 cut(s) 28, 557, 559
HinP1I GCGC 3 cut(s) 28, 557, 559
HincII GTYRAC 2 cut(s) 508, 847
HindII GTYRAC 2 cut(s) 508, 847
HinfI GANTC 7 cut(s) 173, 401, 592, 655, 905, 1036, 1046
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 4 cut(s) 242, 508, 847, 877
Hpy188I TCNGA 5 cut(s) 117, 263, 597, 666, 838
Hpy188III TCNNGA 4 cut(s) 177, 284, 398, 652
Hpy8I GTNNAC 4 cut(s) 242, 508, 847, 877
HpyAV CCTTC 4 cut(s) 227, 245, 323, 1006
HpyCH4III ACNGT 3 cut(s) 133, 281, 824
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 9 cut(s) 122, 160, 214, 427, 581, 617, 676, 757, 986
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 463
HpyF3I CTNAG 3 cut(s) 414, 451, 594
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 7 cut(s) 44, 160, 574, 592, 765, 770, 886
HspAI GCGC 3 cut(s) 28, 557, 559
Ksp22I TGATCA 4 cut(s) 360, 838, 925, 973
Kzo9I GATC 6 cut(s) 6, 360, 604, 838, 925, 973
LguI GCTCTTC 1 cut(s) 908
LmnI GCTCC 2 cut(s) 289, 1057
Lsp1109I GCAGC 1 cut(s) 970
MaeI CTAG 3 cut(s) 177, 407, 1017
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 43
MalI GATC 6 cut(s) 8, 362, 606, 840, 927, 975
MboI GATC 6 cut(s) 6, 360, 604, 838, 925, 973
MboII GAAGA 4 cut(s) 452, 679, 782, 925
MfeI CAATTG 2 cut(s) 161, 929
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 1 cut(s) 1005
MluCI AATT 7 cut(s) 161, 198, 264, 804, 929, 937, 1062
MlyI GAGTC 3 cut(s) 167, 586, 899
MseI TTAA 2 cut(s) 267, 308
MslI CAYNNNNRTG 1 cut(s) 771
MspR9I CCNGG 1 cut(s) 1072
MunI CAATTG 2 cut(s) 161, 929
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 1 cut(s) 1072
MvnI CGCG 1 cut(s) 559
MwoI GCNNNNNNNGC 2 cut(s) 424, 463
NcoI CCATGG 1 cut(s) 570
NdeII GATC 6 cut(s) 6, 360, 604, 838, 925, 973
NlaIII CATG 7 cut(s) 44, 160, 574, 592, 765, 770, 886
NlaIV GGNNCC 2 cut(s) 957, 1003
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 44
PauI GCGCGC 1 cut(s) 557
PceI AGGCCT 1 cut(s) 112
PciI ACATGT 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 908
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 4 cut(s) 401, 655, 1036, 1046
PkrI GCNGC 1 cut(s) 985
PleI GAGTC 3 cut(s) 167, 586, 899
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 3 cut(s) 167, 586, 899
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 956
PscI ACATGT 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 956
Psp6I CCWGG 1 cut(s) 1070
PspCI CACGTG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 1070
PspN4I GGNNCC 2 cut(s) 957, 1003
PspOMI GGGCCC 1 cut(s) 1001
PspPI GGNCC 4 cut(s) 703, 956, 1001, 1002
PspPPI RGGWCCY 1 cut(s) 956
PstI CTGCAG 1 cut(s) 988
PstNI CAGNNNCTG 1 cut(s) 822
PsuI RGATCY 1 cut(s) 604
PteI GCGCGC 1 cut(s) 557
RsaI GTAC 2 cut(s) 324, 832
RsaNI GTAC 2 cut(s) 323, 831
RseI CAYNNNNRTG 1 cut(s) 771
SapI GCTCTTC 1 cut(s) 908
SaqAI TTAA 2 cut(s) 267, 308
SatI GCNGC 1 cut(s) 984
Sau3AI GATC 6 cut(s) 6, 360, 604, 838, 925, 973
Sau96I GGNCC 4 cut(s) 703, 956, 1001, 1002
SchI GAGTC 3 cut(s) 167, 586, 899
ScrFI CCNGG 1 cut(s) 1072
SduI GDGCHC 1 cut(s) 1005
SfcI CTRYAG 2 cut(s) 242, 984
SinI GGWCC 1 cut(s) 956
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 7 cut(s) 161, 198, 264, 804, 929, 937, 1062
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 3 cut(s) 220, 503, 1075
SspI AATATT 1 cut(s) 565
SspMI CTAG 3 cut(s) 177, 407, 1017
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 1070
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 3 cut(s) 133, 281, 824
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 127
TasI AATT 7 cut(s) 161, 198, 264, 804, 929, 937, 1062
TfiI GAWTC 4 cut(s) 401, 655, 1036, 1046
Tru1I TTAA 2 cut(s) 267, 308
Tru9I TTAA 2 cut(s) 267, 308
TscAI CASTG 4 cut(s) 350, 434, 624, 678
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 983
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 4 cut(s) 566, 656, 783, 876
TspRI CASTG 4 cut(s) 350, 434, 624, 678
VpaK11BI GGWCC 1 cut(s) 956
VspI ATTAAT 1 cut(s) 267
XagI CCTNNNNNAGG 1 cut(s) 52
XbaI TCTAGA 1 cut(s) 176
XceI RCATGY 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 241
XspI CTAG 3 cut(s) 177, 407, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.