AT1G08940

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010048
Physical Location & Seq
Forward (+)
2877430 .. 2879169
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G08940.1

Sequence Viewer

Length: 846 bp
ATGGTGAATGAGAAGAAGATGCTCCCAAAACGCATCATACTAATGCGTCACGGTGAGTCAGCCGGTAACATCGACGCAGGGGCTTACGCGACCACACCCGATCACAAGATCCCTCTAACGGAAGAAGGAAGAGCGCAAGCGCGTGAGGCCGGGAAGAAAATGAGAGCCCTCATATCAACCCAAAGCGGCGGTGCGTGTGGAGAGAATTGGCGCGTGTACTTCTACGTGTCGCCTTACGAGAGAACGAGGACGACTTTGAGGGAAGTAGGAAAAGGATTCTCGAGGAAGCGCGTGATAGGAGTGAGGGAAGAGTGTAGGATCAGAGAACAAGATTTTGGGAATTTTCAAGTGGAAGAGAGGATGAGAGTTGTGAAGGAGACGAGGGAACGTTTCGGTAGATTTTTCTATCGTTTTCCCGAAGGTGAATCCGCCGCCGACGTCTACGATCGTGTTTCTAGTTTTCTGGAGTCTATGTGGAGAGACGTGGACATGAACAGGCATCAAGTGGATCCATCAAGTGAACTAAACCTAGTGATTGTGTCCCACGGACTGACATCTCGAGTGTTTCTAACAAAATGGTTCAAGTGGACGGTGGCAGAGTTCGAGCGGTTGAATAACTTTGGGAACTGCGAGTTCAGAGTGATGGAGTTGGGTGCGAGCGGAGAGTACACTTTCGCGATACACCACAGTGAAGAAGAGATGTTGGATTGGGGCATGTCTAAAGATATGATTGATGATCAAAAGGATCGTGTTGATGGGTGTCGTGTAACAACATCAAACGATTCTTGTTCATTGCATCTCAATGAGTATTTTGATTTGTTAGATGTCACTGATGATGAAGAATGA

Protein Analysis

281

Amino Acids

32.68

Weight (kDa)

5.55

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 12 - 220 2.6e-26 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 441
AccBSI CCGCTC 2 cut(s) 607, 660
AccI GTMKAC 1 cut(s) 441
AccII CGCG 5 cut(s) 89, 142, 213, 291, 677
AciI CCGC 6 cut(s) 186, 189, 429, 432, 607, 660
AclI AACGTT 1 cut(s) 388
AclWI GGATC 5 cut(s) 103, 326, 503, 516, 753
AcsI RAATTY 1 cut(s) 340
AcyI GRCGYC 1 cut(s) 438
AfaI GTAC 2 cut(s) 218, 668
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 347, 583, 613
AjiI CACGTC 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AleI CACNNNNGTG 1 cut(s) 687
Alw26I GTCTC 2 cut(s) 371, 474
AlwI GGATC 5 cut(s) 103, 326, 503, 516, 753
Ama87I CYCGRG 2 cut(s) 280, 558
AoxI GGCC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 340
AspLEI GCGC 4 cut(s) 136, 142, 213, 291
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 3 cut(s) 16, 65, 434
AvaI CYCGRG 2 cut(s) 280, 558
BamHI GGATCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 169
BccI CCATC 3 cut(s) 520, 637, 749
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 371, 474
BfaI CTAG 2 cut(s) 456, 530
BisI GCNGC 2 cut(s) 187, 432
BlsI GCNGC 2 cut(s) 188, 433
Bme1390I CCNGG 1 cut(s) 151
BmeT110I CYCGRG 2 cut(s) 280, 558
BmgBI CACGTC 1 cut(s) 484
BmiI GGNNCC 1 cut(s) 510
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 4 cut(s) 9, 42, 508, 805
BpmI CTGGAG 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 151
BsaAI YACGTR 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 544
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 791
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 791
Bsh1236I CGCG 5 cut(s) 89, 142, 213, 291, 677
Bsh1285I CGRYCG 1 cut(s) 448
BshFI GGCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 448
BsiHKCI CYCGRG 2 cut(s) 280, 558
BsiSI CCGG 2 cut(s) 63, 150
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 2 cut(s) 371, 474
BsmBI CGTCTC 2 cut(s) 371, 474
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 1 cut(s) 149
BsoBI CYCGRG 2 cut(s) 280, 558
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
Bsp68I TCGCGA 1 cut(s) 677
BspACI CCGC 6 cut(s) 186, 189, 429, 432, 607, 660
BspANI GGCC 1 cut(s) 149
BspFNI CGCG 5 cut(s) 89, 142, 213, 291, 677
BspLI GGNNCC 1 cut(s) 510
BspPI GGATC 5 cut(s) 103, 326, 503, 516, 753
BspQI GCTCTTC 1 cut(s) 124
BsrBI CCGCTC 2 cut(s) 607, 660
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 1 cut(s) 62
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
BssNI GRCGYC 1 cut(s) 438
Bst4CI ACNGT 3 cut(s) 53, 592, 689
Bst6I CTCTTC 4 cut(s) 124, 303, 348, 690
BstACI GRCGYC 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 138, 658
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 1 cut(s) 366
BstFNI CGCG 5 cut(s) 89, 142, 213, 291, 677
BstHHI GCGC 4 cut(s) 136, 142, 213, 291
BstKTI GATC 7 cut(s) 103, 111, 321, 448, 511, 739, 748
BstMAI GTCTC 2 cut(s) 371, 474
BstMBI GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
BstMCI CGRYCG 1 cut(s) 448
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 149
BstUI CGCG 5 cut(s) 89, 142, 213, 291, 677
BstX2I RGATCY 2 cut(s) 108, 508
BstYI RGATCY 2 cut(s) 108, 508
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 544
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 694, 828
BtuMI TCGCGA 1 cut(s) 677
Cac8I GCNNGC 2 cut(s) 138, 658
CfoI GCGC 4 cut(s) 136, 142, 213, 291
Cfr10I RCCGGY 1 cut(s) 62
CseI GACGC 2 cut(s) 35, 83
Csp6I GTAC 2 cut(s) 217, 667
CviAII CATG 2 cut(s) 490, 715
CviJI RGCY 4 cut(s) 62, 83, 149, 167
CviKI_1 RGCY 4 cut(s) 62, 83, 149, 167
CviQI GTAC 2 cut(s) 217, 667
DpnI GATC 7 cut(s) 102, 110, 320, 447, 510, 738, 747
DpnII GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
Eam1104I CTCTTC 4 cut(s) 124, 303, 348, 690
EarI CTCTTC 4 cut(s) 124, 303, 348, 690
EciI GGCGGA 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 169
Eco88I CYCGRG 2 cut(s) 280, 558
EcoT38I GRGCYC 1 cut(s) 169
Esp3I CGTCTC 2 cut(s) 371, 474
FaeI CATG 2 cut(s) 493, 718
FaiI YATR 6 cut(s) 38, 173, 473, 491, 716, 728
FaqI GGGAC 1 cut(s) 526
FatI CATG 2 cut(s) 489, 714
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 187, 432
FokI GGATG 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 187, 432
FspBI CTAG 2 cut(s) 456, 530
GlaI GCGC 4 cut(s) 135, 141, 212, 290
GluI GCNGC 2 cut(s) 187, 432
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 1 cut(s) 149
HapII CCGG 2 cut(s) 63, 150
HgaI GACGC 2 cut(s) 35, 83
HhaI GCGC 4 cut(s) 136, 142, 213, 291
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 2 cut(s) 493, 718
Hin6I GCGC 4 cut(s) 134, 140, 211, 289
HinP1I GCGC 4 cut(s) 134, 140, 211, 289
HinfI GANTC 5 cut(s) 56, 276, 425, 467, 782
HpaII CCGG 2 cut(s) 63, 150
HphI GGTGA 3 cut(s) 16, 65, 434
Hpy166II GTNNAC 6 cut(s) 217, 442, 487, 521, 588, 669
Hpy188I TCNGA 2 cut(s) 323, 638
Hpy188III TCNNGA 5 cut(s) 280, 416, 464, 558, 676
Hpy8I GTNNAC 6 cut(s) 217, 442, 487, 521, 588, 669
Hpy99I CGWCG 2 cut(s) 77, 440
HpyAV CCTTC 3 cut(s) 119, 367, 413
HpyCH4III ACNGT 3 cut(s) 53, 592, 689
HpyCH4IV ACGT 4 cut(s) 225, 388, 438, 483
HpyCH4V TGCA 1 cut(s) 796
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpySE526I ACGT 4 cut(s) 225, 388, 438, 483
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 2 cut(s) 493, 718
HspAI GCGC 4 cut(s) 134, 140, 211, 289
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
LguI GCTCTTC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 27
LpnPI CCDG 5 cut(s) 63, 76, 163, 449, 481
LweI GCATC 4 cut(s) 9, 42, 508, 805
MaeI CTAG 2 cut(s) 456, 530
MaeII ACGT 4 cut(s) 225, 388, 438, 483
MaeIII GTNAC 4 cut(s) 47, 65, 766, 826
MalI GATC 7 cut(s) 102, 110, 320, 447, 510, 738, 747
MbiI CCGCTC 2 cut(s) 607, 660
MboI GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
MboII GAAGA 9 cut(s) 25, 28, 134, 141, 166, 320, 365, 704, 707
MflI RGATCY 2 cut(s) 108, 508
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 2 cut(s) 205, 340
MlyI GAGTC 2 cut(s) 65, 476
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 9 cut(s) 123, 139, 179, 240, 252, 276, 297, 351, 375
MslI CAYNNNNRTG 3 cut(s) 41, 687, 801
MspI CCGG 2 cut(s) 63, 150
MspR9I CCNGG 1 cut(s) 151
MvnI CGCG 5 cut(s) 89, 142, 213, 291, 677
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 1 cut(s) 151
NdeII GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
NlaIII CATG 2 cut(s) 493, 718
NlaIV GGNNCC 1 cut(s) 510
NmuCI GTSAC 2 cut(s) 47, 826
NruI TCGCGA 1 cut(s) 677
NspI RCATGY 1 cut(s) 718
OliI CACNNNNGTG 1 cut(s) 687
PaeR7I CTCGAG 2 cut(s) 280, 558
PciSI GCTCTTC 1 cut(s) 124
PfeI GAWTC 3 cut(s) 276, 425, 782
PkrI GCNGC 2 cut(s) 188, 433
Ple19I CGATCG 1 cut(s) 448
PleI GAGTC 2 cut(s) 64, 475
PpsI GAGTC 2 cut(s) 64, 475
Ppu21I YACGTR 1 cut(s) 226
Psp1406I AACGTT 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 510
PsuI RGATCY 2 cut(s) 108, 508
PvuI CGATCG 1 cut(s) 448
RruI TCGCGA 1 cut(s) 677
RsaI GTAC 2 cut(s) 218, 668
RsaNI GTAC 2 cut(s) 217, 667
RseI CAYNNNNRTG 3 cut(s) 41, 687, 801
SapI GCTCTTC 1 cut(s) 124
SatI GCNGC 2 cut(s) 187, 432
Sau3AI GATC 7 cut(s) 100, 108, 318, 445, 508, 736, 745
SchI GAGTC 2 cut(s) 65, 476
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 169
SetI ASST 6 cut(s) 228, 391, 424, 441, 486, 531
SfaNI GCATC 4 cut(s) 9, 42, 508, 805
Sfr274I CTCGAG 2 cut(s) 280, 558
SlaI CTCGAG 2 cut(s) 280, 558
SmiMI CAYNNNNRTG 3 cut(s) 41, 687, 801
SmlI CTYRAG 2 cut(s) 280, 558
SmoI CTYRAG 2 cut(s) 280, 558
Sse9I AATT 2 cut(s) 205, 340
SsiI CCGC 6 cut(s) 186, 189, 429, 432, 607, 660
SspMI CTAG 2 cut(s) 456, 530
StyD4I CCNGG 1 cut(s) 149
TaaI ACNGT 3 cut(s) 53, 592, 689
TaiI ACGT 4 cut(s) 228, 391, 441, 486
TaqI TCGA 4 cut(s) 72, 281, 559, 603
TasI AATT 2 cut(s) 205, 340
TatI WGTACW 2 cut(s) 216, 666
TauI GCSGC 2 cut(s) 189, 434
TfiI GAWTC 3 cut(s) 276, 425, 782
TscAI CASTG 2 cut(s) 694, 835
TseFI GTSAC 2 cut(s) 47, 826
Tsp45I GTSAC 2 cut(s) 47, 826
TspDTI ATGAA 2 cut(s) 506, 780
TspGWI ACGGA 2 cut(s) 134, 561
TspRI CASTG 2 cut(s) 694, 835
XapI RAATTY 1 cut(s) 340
XceI RCATGY 1 cut(s) 718
XhoI CTCGAG 2 cut(s) 280, 558
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 2 cut(s) 456, 530
ZraI GACGTC 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.