Rroxscaffold_6G00430790

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010048
Physical Location & Seq
Forward (+)
50901452 .. 50903318
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430790.1

Sequence Viewer

Length: 984 bp
ATGCATGGGAGACCATCTTCTTCGTCTTCTACGTCTTCGTCTTCTTGTTCTCTACAACCATGTCTGCCGAAACGGATAATCCTAATGCGTCACGCCGAATCCCAAGGCAATCTGGACATGGCGGCCTACACGACCACCCCGGACAACAAAATCCCGCTCACCGAACAGGGCCTGGCCCAGGCCCAGCTCGCCGGGGCCCAACTCCGCCGCGTCGTCTCCGCCGACAACGACTCCAACCACCCCGAGTGGCGCGTCTACTTCTACGTCTCCCCTTACGAGCGCACCCGCTCCACGCTCTCCCAGATCGGACGGTCCTTCTCCCGGACACGTGTCATCGGCGTCCGCGAGGAGTGCCGGATAAGGGAGCAGGATTTCGGGAATTTTCAGGTGGAGGAGAGAATGAAGGCCATTAAGCAGACCAGGGAGAAGTTCGGCAGATTCTTCTATCGGTTTCCGGAGGGGAGTCCGCCGCTGATGTTTACGATCGTGTTTCCATTTTACTGCGTTTTGTCTACAGAATTGAATGTAGAGCTAATAAGACAGATGATTGCAGGTTTTCTTGAATCTTTATGGAGGGACATAGACATGAACAGGCTTCGCCACAACCCCTGCCATGACCTTAATCTTGTAATTGTATCACATGGGTTAACATCGCGTGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTAGAGCAATTCGAGCACCTAAACAACTTTGGAAACTGTGAATTTCGAGTGATGCAATTAGGAGCTGGCGGAGAGTATAGCTTAGCAGTCCATCACACAGAGGAAGAGCTAATAGAATGGGGTCTTTCACCTGAAATGATAGCTGACCAAAAGTGGCGAGCCCATTCGGGCAAGGGTTGGACTGACCGTTGCCCCTGGTTCATTGATTCATTCTTTGACAAGCTAGCCGACTCTGATGACACAGACAGTGACGATGTTTGGCATGAAACCAATGATAAAGTTCAGCAATCAAATTGA

Protein Analysis

327

Amino Acids

37.93

Weight (kDa)

6.02

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 26 - 156 2.4e-13 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 542
AccBSI CCGCTC 2 cut(s) 157, 288
AccI GTMKAC 2 cut(s) 255, 512
AccII CGCG 4 cut(s) 210, 252, 345, 655
AccIII TCCGGA 1 cut(s) 454
AcsI RAATTY 2 cut(s) 379, 728
AcvI CACGTG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 339
AfiI CCNNNNNNNGG 3 cut(s) 178, 321, 361
AflIII ACRYGT 2 cut(s) 326, 328
AgsI TTSAA 3 cut(s) 523, 563, 679
AjnI CCWGG 4 cut(s) 171, 177, 419, 881
AluBI AGCT 7 cut(s) 187, 532, 752, 768, 796, 830, 910
AluI AGCT 7 cut(s) 187, 532, 752, 768, 796, 830, 910
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 3 cut(s) 4, 220, 271
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 6 cut(s) 123, 169, 174, 180, 195, 405
ApaI GGGCCC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 379, 728
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 2 cut(s) 252, 282
AspS9I GGNCC 6 cut(s) 169, 175, 181, 195, 196, 312
AsuC2I CCSGG 3 cut(s) 140, 193, 322
AsuHPI GGTGA 2 cut(s) 151, 807
AsuNHI GCTAGC 1 cut(s) 910
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 199
BanII GRGCYC 2 cut(s) 199, 850
BbrPI CACGTG 1 cut(s) 329
BbsI GAAGAC 4 cut(s) 18, 27, 33, 652
Bbv12I GWGCWC 1 cut(s) 705
BccI CCATC 2 cut(s) 22, 786
BciT130I CCWGG 4 cut(s) 173, 179, 421, 883
BcnI CCSGG 3 cut(s) 140, 193, 322
BcoDI GTCTC 3 cut(s) 4, 220, 271
BfaI CTAG 1 cut(s) 911
BfmI CTRYAG 1 cut(s) 513
BfuAI ACCTGC 1 cut(s) 542
BisI GCNGC 3 cut(s) 123, 208, 470
BlpI GCTNAGC 1 cut(s) 769
BlsI GCNGC 3 cut(s) 124, 209, 471
Bme1390I CCNGG 7 cut(s) 140, 173, 179, 193, 322, 421, 883
Bme18I GGWCC 1 cut(s) 312
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 6 cut(s) 169, 175, 181, 195, 196, 312
BmiI GGNNCC 2 cut(s) 196, 197
BmrFI CCNGG 7 cut(s) 140, 173, 179, 193, 322, 421, 883
BmsI GCATC 1 cut(s) 729
BmtI GCTAGC 1 cut(s) 914
BoxI GACNNNNGTC 1 cut(s) 329
BpiI GAAGAC 4 cut(s) 18, 27, 33, 652
Bpu1102I GCTNAGC 1 cut(s) 769
BpuMI CCSGG 3 cut(s) 140, 193, 322
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 6 cut(s) 103, 138, 177, 192, 420, 881
BsaWI WCCGGW 1 cut(s) 454
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 321, 361
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 4 cut(s) 173, 179, 421, 883
BseDI CCNNGG 6 cut(s) 103, 138, 177, 192, 420, 881
BseLI CCNNNNNNNGG 3 cut(s) 178, 321, 361
BseRI GAGGAG 2 cut(s) 362, 407
BseSI GKGCMC 1 cut(s) 199
BseYI CCCAGC 1 cut(s) 183
Bsh1236I CGCG 4 cut(s) 210, 252, 345, 655
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 6 cut(s) 125, 171, 176, 182, 197, 407
BsiEI CGRYCG 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 5 cut(s) 140, 192, 322, 355, 455
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 3 cut(s) 178, 321, 361
BsmAI GTCTC 3 cut(s) 4, 220, 271
BsmBI CGTCTC 2 cut(s) 220, 271
BsmFI GGGAC 1 cut(s) 590
BsnI GGCC 6 cut(s) 125, 171, 176, 182, 197, 407
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 242
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 199, 705, 850
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 2 cut(s) 303, 483
Bsp1720I GCTNAGC 1 cut(s) 769
BspANI GGCC 6 cut(s) 125, 171, 176, 182, 197, 407
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 4 cut(s) 210, 252, 345, 655
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 2 cut(s) 196, 197
BspMI ACCTGC 1 cut(s) 542
BspOI GCTAGC 1 cut(s) 914
BspQI GCTCTTC 1 cut(s) 786
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 2 cut(s) 157, 288
BssECI CCNNGG 6 cut(s) 103, 138, 177, 192, 420, 881
BssMI GATC 2 cut(s) 303, 483
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 4 cut(s) 173, 179, 421, 883
Bst4CI ACNGT 5 cut(s) 312, 688, 725, 875, 935
Bst6I CTCTTC 1 cut(s) 786
BstACI GRCGYC 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 4 cut(s) 189, 754, 846, 912
BstDEI CTNAG 1 cut(s) 769
BstENI CCTNNNNNAGG 1 cut(s) 176
BstFNI CGCG 4 cut(s) 210, 252, 345, 655
BstHHI GCGC 2 cut(s) 252, 282
BstKTI GATC 2 cut(s) 306, 486
BstMAI GTCTC 3 cut(s) 4, 220, 271
BstMBI GATC 2 cut(s) 303, 483
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 3 cut(s) 188, 351, 700
BstNI CCWGG 4 cut(s) 173, 179, 421, 883
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 7 cut(s) 138, 171, 177, 191, 320, 419, 881
BstSFI CTRYAG 1 cut(s) 513
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 4 cut(s) 210, 252, 345, 655
BstV2I GAAGAC 4 cut(s) 18, 27, 33, 652
BsuRI GGCC 6 cut(s) 125, 171, 176, 182, 197, 407
BtgZI GCGATG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 940
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 4 cut(s) 189, 754, 846, 912
CaiI CAGNNNCTG 1 cut(s) 172
CciI TCATGA 1 cut(s) 666
CfoI GCGC 2 cut(s) 252, 282
Cfr13I GGNCC 6 cut(s) 169, 175, 181, 195, 196, 312
CseI GACGC 4 cut(s) 77, 199, 241, 328
CviAII CATG 8 cut(s) 5, 60, 118, 586, 614, 641, 667, 950
DdeI CTNAG 1 cut(s) 769
DpnI GATC 2 cut(s) 305, 485
DpnII GATC 2 cut(s) 303, 483
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 4 cut(s) 194, 208, 456, 771
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 199, 850
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 312
Eco72I CACGTG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 242
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoO109I RGGNCCY 2 cut(s) 169, 195
EcoRII CCWGG 4 cut(s) 171, 177, 419, 881
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 199, 850
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 2 cut(s) 220, 271
FaeI CATG 8 cut(s) 8, 63, 121, 589, 617, 644, 670, 953
FaqI GGGAC 1 cut(s) 590
FatI CATG 8 cut(s) 4, 59, 117, 585, 613, 640, 666, 949
FauI CCCGC 2 cut(s) 162, 293
FblI GTMKAC 2 cut(s) 255, 512
Fnu4HI GCNGC 3 cut(s) 123, 208, 470
FriOI GRGCYC 2 cut(s) 199, 850
Fsp4HI GCNGC 3 cut(s) 123, 208, 470
FspBI CTAG 1 cut(s) 911
GlaI GCGC 2 cut(s) 251, 281
GluI GCNGC 3 cut(s) 123, 208, 470
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 6 cut(s) 125, 171, 176, 182, 197, 407
HapII CCGG 5 cut(s) 140, 192, 322, 355, 455
HgaI GACGC 4 cut(s) 77, 199, 241, 328
HhaI GCGC 2 cut(s) 252, 282
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 8 cut(s) 8, 63, 121, 589, 617, 644, 670, 953
Hin6I GCGC 2 cut(s) 250, 280
HinP1I GCGC 2 cut(s) 250, 280
HincII GTYRAC 1 cut(s) 648
HindII GTYRAC 1 cut(s) 648
HinfI GANTC 7 cut(s) 98, 230, 438, 463, 563, 893, 917
HpaI GTTAAC 1 cut(s) 648
HpaII CCGG 5 cut(s) 140, 192, 322, 355, 455
HphI GGTGA 2 cut(s) 151, 807
Hpy166II GTNNAC 5 cut(s) 256, 480, 513, 648, 684
Hpy188I TCNGA 2 cut(s) 308, 922
Hpy188III TCNNGA 5 cut(s) 113, 376, 455, 560, 667
Hpy8I GTNNAC 5 cut(s) 256, 480, 513, 648, 684
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 325, 397
HpyCH4III ACNGT 5 cut(s) 312, 688, 725, 875, 935
HpyCH4IV ACGT 3 cut(s) 32, 264, 328
HpyCH4V TGCA 3 cut(s) 4, 551, 742
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 351, 700
HpyF3I CTNAG 1 cut(s) 769
HpySE526I ACGT 3 cut(s) 32, 264, 328
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 8 cut(s) 8, 63, 121, 589, 617, 644, 670, 953
HspAI GCGC 2 cut(s) 250, 280
Kpn2I TCCGGA 1 cut(s) 454
KspAI GTTAAC 1 cut(s) 648
Kzo9I GATC 2 cut(s) 303, 483
LguI GCTCTTC 1 cut(s) 786
LmnI GCTCC 3 cut(s) 293, 364, 749
LweI GCATC 1 cut(s) 729
MaeI CTAG 1 cut(s) 911
MaeII ACGT 3 cut(s) 32, 264, 328
MaeIII GTNAC 2 cut(s) 89, 935
MalI GATC 2 cut(s) 305, 485
MbiI CCGCTC 2 cut(s) 157, 288
MboI GATC 2 cut(s) 303, 483
MboII GAAGA 8 cut(s) 9, 12, 18, 27, 33, 433, 652, 803
MhlI GDGCHC 3 cut(s) 199, 705, 850
MluCI AATT 7 cut(s) 379, 518, 630, 695, 728, 743, 979
MlyI GAGTC 3 cut(s) 224, 472, 911
MmeI TCCRAC 2 cut(s) 258, 845
MnlI CCTC 6 cut(s) 340, 385, 451, 567, 674, 781
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 3 cut(s) 411, 621, 647
MslI CAYNNNNRTG 1 cut(s) 584
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 5 cut(s) 140, 192, 322, 355, 455
MspR9I CCNGG 7 cut(s) 140, 173, 179, 193, 322, 421, 883
MvaI CCWGG 4 cut(s) 173, 179, 421, 883
MvnI CGCG 4 cut(s) 210, 252, 345, 655
MwoI GCNNNNNNNGC 3 cut(s) 188, 351, 700
NciI CCSGG 3 cut(s) 140, 193, 322
NdeII GATC 2 cut(s) 303, 483
NheI GCTAGC 1 cut(s) 910
NlaIII CATG 8 cut(s) 8, 63, 121, 589, 617, 644, 670, 953
NlaIV GGNNCC 2 cut(s) 196, 197
NmuCI GTSAC 2 cut(s) 89, 935
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 786
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 219, 342
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 4 cut(s) 98, 438, 563, 893
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 3 cut(s) 124, 209, 471
Ple19I CGATCG 1 cut(s) 486
PleI GAGTC 3 cut(s) 224, 471, 911
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 3 cut(s) 224, 471, 911
Ppu21I YACGTR 1 cut(s) 329
PshAI GACNNNNGTC 1 cut(s) 329
Psp6I CCWGG 4 cut(s) 171, 177, 419, 881
PspCI CACGTG 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 4 cut(s) 171, 177, 419, 881
PspN4I GGNNCC 2 cut(s) 196, 197
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 6 cut(s) 169, 175, 181, 195, 196, 312
PstNI CAGNNNCTG 1 cut(s) 172
PvuI CGATCG 1 cut(s) 486
RseI CAYNNNNRTG 1 cut(s) 584
SapI GCTCTTC 1 cut(s) 786
SaqAI TTAA 3 cut(s) 411, 621, 647
SatI GCNGC 3 cut(s) 123, 208, 470
Sau3AI GATC 2 cut(s) 303, 483
Sau96I GGNCC 6 cut(s) 169, 175, 181, 195, 196, 312
SchI GAGTC 3 cut(s) 224, 472, 911
ScrFI CCNGG 7 cut(s) 140, 173, 179, 193, 322, 421, 883
SduI GDGCHC 3 cut(s) 199, 705, 850
SfaNI GCATC 1 cut(s) 729
SfcI CTRYAG 1 cut(s) 513
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 584
Sse9I AATT 7 cut(s) 379, 518, 630, 695, 728, 743, 979
SspMI CTAG 1 cut(s) 911
StyD4I CCNGG 7 cut(s) 138, 171, 177, 191, 320, 419, 881
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 5 cut(s) 312, 688, 725, 875, 935
TaiI ACGT 3 cut(s) 35, 267, 331
TaqI TCGA 2 cut(s) 699, 733
TasI AATT 7 cut(s) 379, 518, 630, 695, 728, 743, 979
TauI GCSGC 3 cut(s) 125, 210, 472
TfiI GAWTC 4 cut(s) 98, 438, 563, 893
Tru1I TTAA 3 cut(s) 411, 621, 647
Tru9I TTAA 3 cut(s) 411, 621, 647
TscAI CASTG 1 cut(s) 940
TseFI GTSAC 2 cut(s) 89, 935
Tsp45I GTSAC 2 cut(s) 89, 935
TspDTI ATGAA 6 cut(s) 416, 602, 683, 877, 885, 966
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 1 cut(s) 940
VpaK11BI GGWCC 1 cut(s) 312
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 2 cut(s) 379, 728
XmiI GTMKAC 2 cut(s) 255, 512
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 1 cut(s) 911
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.