Rw3G004190

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010048
Physical Location & Seq
Forward (+)
3686424 .. 3688634
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004190.1

Sequence Viewer

Length: 930 bp
ATGCATGGGAGACCATCTTCTTCGTCTTCTACGTCTTCGTCTTCTTCTTCTTCTCTCCAACCATGTCTCCCGAAACGGATAATCCTAATGCGTCACGCCGAATCCCAAGGCAATCTGGACATGGCGGCCTACACGAGCACCCCGGACAACAAAATCCCGCTCACCGAACAGGGCCTGGCCCAGGCCCAGCTCGCGGGGGCCCAACTCCGCCGCGTCGTCTCCGCCGACAACGACTCCAACCACCCCGAGTGGCGCGTCTACTTCTACGTCTCCCCTTACGAGCGCACCCGCTCCACGCTCTCCCAGATCGGACGGTCCTTCTCCCGGAAACGTGTCATCGGCGTCCGCGAGGAGTGCCGGATAAGGGAGCAGGATTTCGGGAATTTTCAGGTGGAGGAGAGAATGAAGGCCATTAAGCAGACGAGGGAGAAGTTCGGCAGATTCTTCTATCGGTTTCCGGAGGGGGAGTCCGCCGCTGATGTTTACGATCGTGTTTCCAGTTTTCTTGAATCTTTATGGAGGGACATAGACATGAACAGGCTTCGCCACAATCCTTGCCATGACCTTAATCTTGTAATTGTATCACATGGGTTAACATCGCGTGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTAGAGCAATTCGAGCACCTATACAACTTTGGAAACTGTGAATTTCGAGTGATGCAATTAGGAGCTGGCGGAGAGTATAGCTTAGCAGTCCATCACACAGAGGAAGAGCTAATAGAATGGGGTCTTTCACCTGAAATGATAGCTGACCAAAAGTGGCGAGCCCATTCGGGCAAGGGTTTGACTGACCGTTGCCCCTGGTTCATTGATTCATTCTTTGACAAGCTAGCCGACTCTGATGACACAGACAGTGACGATGTTTGGCATGAAACCAATGACAAAGTTCAGCAATCAAATTGA

Protein Analysis

309

Amino Acids

35.76

Weight (kDa)

6.02

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 27 - 236 5.2e-26 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 160, 291
AccI GTMKAC 1 cut(s) 258
AccII CGCG 5 cut(s) 194, 213, 255, 348, 601
AccIII TCCGGA 1 cut(s) 457
AcsI RAATTY 2 cut(s) 382, 674
AcyI GRCGYC 1 cut(s) 342
AfiI CCNNNNNNNGG 4 cut(s) 181, 193, 324, 364
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 2 cut(s) 509, 625
AjnI CCWGG 3 cut(s) 174, 180, 827
AluBI AGCT 6 cut(s) 190, 698, 714, 742, 776, 856
AluI AGCT 6 cut(s) 190, 698, 714, 742, 776, 856
Alw21I GWGCWC 2 cut(s) 140, 651
Alw26I GTCTC 4 cut(s) 4, 71, 223, 274
AlwNI CAGNNNCTG 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 6 cut(s) 126, 172, 177, 183, 198, 408
ApaI GGGCCC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 382, 674
Asp700I GAANNNNTTC 1 cut(s) 620
AspLEI GCGC 2 cut(s) 255, 285
AspS9I GGNCC 6 cut(s) 172, 178, 184, 198, 199, 315
AsuC2I CCSGG 2 cut(s) 143, 325
AsuHPI GGTGA 2 cut(s) 154, 753
AsuNHI GCTAGC 1 cut(s) 856
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 202
BanII GRGCYC 2 cut(s) 202, 796
BauI CACGAG 1 cut(s) 133
BbsI GAAGAC 4 cut(s) 18, 27, 33, 598
Bbv12I GWGCWC 2 cut(s) 140, 651
BccI CCATC 2 cut(s) 22, 732
BciT130I CCWGG 3 cut(s) 176, 182, 829
BcnI CCSGG 2 cut(s) 143, 325
BcoDI GTCTC 4 cut(s) 4, 71, 223, 274
BfaI CTAG 1 cut(s) 857
BisI GCNGC 3 cut(s) 126, 211, 474
BlpI GCTNAGC 1 cut(s) 715
BlsI GCNGC 3 cut(s) 127, 212, 475
Bme1390I CCNGG 5 cut(s) 143, 176, 182, 325, 829
Bme18I GGWCC 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 6 cut(s) 172, 178, 184, 198, 199, 315
BmiI GGNNCC 2 cut(s) 199, 200
BmrFI CCNGG 5 cut(s) 143, 176, 182, 325, 829
BmsI GCATC 1 cut(s) 675
BmtI GCTAGC 1 cut(s) 860
BpiI GAAGAC 4 cut(s) 18, 27, 33, 598
Bpu1102I GCTNAGC 1 cut(s) 715
BpuMI CCSGG 2 cut(s) 143, 325
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 4 cut(s) 106, 141, 180, 827
BsaWI WCCGGW 1 cut(s) 457
BsaXI ACNNNNNCTCC 6 cut(s) 51, 81, 218, 248, 452, 482
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 193, 324, 364
Bse1I ACTGG 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 3 cut(s) 176, 182, 829
BseDI CCNNGG 4 cut(s) 106, 141, 180, 827
BseLI CCNNNNNNNGG 4 cut(s) 181, 193, 324, 364
BseNI ACTGG 1 cut(s) 498
BseRI GAGGAG 2 cut(s) 365, 410
BseSI GKGCMC 1 cut(s) 202
BseYI CCCAGC 1 cut(s) 186
Bsh1236I CGCG 5 cut(s) 194, 213, 255, 348, 601
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 6 cut(s) 128, 174, 179, 185, 200, 410
BsiEI CGRYCG 1 cut(s) 490
BsiHKAI GWGCWC 2 cut(s) 140, 651
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 4 cut(s) 143, 325, 358, 458
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 4 cut(s) 181, 193, 324, 364
BsmAI GTCTC 4 cut(s) 4, 71, 223, 274
BsmBI CGTCTC 2 cut(s) 223, 274
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 6 cut(s) 128, 174, 179, 185, 200, 410
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 245
Bsp120I GGGCCC 1 cut(s) 198
Bsp1286I GDGCHC 4 cut(s) 140, 202, 651, 796
Bsp13I TCCGGA 1 cut(s) 457
Bsp143I GATC 2 cut(s) 306, 487
Bsp1720I GCTNAGC 1 cut(s) 715
BspANI GGCC 6 cut(s) 128, 174, 179, 185, 200, 410
BspEI TCCGGA 1 cut(s) 457
BspFNI CGCG 5 cut(s) 194, 213, 255, 348, 601
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 199, 200
BspOI GCTAGC 1 cut(s) 860
BspQI GCTCTTC 1 cut(s) 732
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 2 cut(s) 160, 291
BsrI ACTGG 1 cut(s) 498
BssECI CCNNGG 4 cut(s) 106, 141, 180, 827
BssMI GATC 2 cut(s) 306, 487
BssNI GRCGYC 1 cut(s) 342
BssSI CACGAG 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 133
Bst2UI CCWGG 3 cut(s) 176, 182, 829
Bst4CI ACNGT 5 cut(s) 315, 634, 671, 821, 881
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 4 cut(s) 192, 700, 792, 858
BstDEI CTNAG 1 cut(s) 715
BstENI CCTNNNNNAGG 1 cut(s) 179
BstFNI CGCG 5 cut(s) 194, 213, 255, 348, 601
BstHHI GCGC 2 cut(s) 255, 285
BstKTI GATC 2 cut(s) 309, 490
BstMAI GTCTC 4 cut(s) 4, 71, 223, 274
BstMBI GATC 2 cut(s) 306, 487
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 3 cut(s) 191, 354, 646
BstNI CCWGG 3 cut(s) 176, 182, 829
BstSCI CCNGG 5 cut(s) 141, 174, 180, 323, 827
BstSLI GKGCMC 1 cut(s) 202
BstUI CGCG 5 cut(s) 194, 213, 255, 348, 601
BstV2I GAAGAC 4 cut(s) 18, 27, 33, 598
BsuRI GGCC 6 cut(s) 128, 174, 179, 185, 200, 410
BtgZI GCGATG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 886
Cac8I GCNNGC 4 cut(s) 192, 700, 792, 858
CaiI CAGNNNCTG 1 cut(s) 175
CciI TCATGA 1 cut(s) 612
CfoI GCGC 2 cut(s) 255, 285
Cfr13I GGNCC 6 cut(s) 172, 178, 184, 198, 199, 315
CseI GACGC 4 cut(s) 80, 202, 244, 331
CspCI CAANNNNNGTGG 2 cut(s) 536, 571
CviAII CATG 8 cut(s) 5, 63, 121, 532, 560, 587, 613, 896
DdeI CTNAG 1 cut(s) 715
DpnI GATC 2 cut(s) 308, 489
DpnII GATC 2 cut(s) 306, 487
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
EciI GGCGGA 4 cut(s) 197, 211, 460, 717
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 2 cut(s) 202, 796
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoO109I RGGNCCY 2 cut(s) 172, 198
EcoRII CCWGG 3 cut(s) 174, 180, 827
EcoT14I CCWWGG 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 202, 796
ErhI CCWWGG 1 cut(s) 106
Esp3I CGTCTC 2 cut(s) 223, 274
FaeI CATG 8 cut(s) 8, 66, 124, 535, 563, 590, 616, 899
FaqI GGGAC 1 cut(s) 536
FatI CATG 8 cut(s) 4, 62, 120, 531, 559, 586, 612, 895
FauI CCCGC 3 cut(s) 165, 187, 296
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 3 cut(s) 126, 211, 474
FriOI GRGCYC 2 cut(s) 202, 796
Fsp4HI GCNGC 3 cut(s) 126, 211, 474
FspBI CTAG 1 cut(s) 857
GlaI GCGC 2 cut(s) 254, 284
GluI GCNGC 3 cut(s) 126, 211, 474
GsaI CCCAGC 1 cut(s) 190
HaeIII GGCC 6 cut(s) 128, 174, 179, 185, 200, 410
HapII CCGG 4 cut(s) 143, 325, 358, 458
HgaI GACGC 4 cut(s) 80, 202, 244, 331
HhaI GCGC 2 cut(s) 255, 285
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 8 cut(s) 8, 66, 124, 535, 563, 590, 616, 899
Hin6I GCGC 2 cut(s) 253, 283
HinP1I GCGC 2 cut(s) 253, 283
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HinfI GANTC 7 cut(s) 101, 233, 441, 467, 509, 839, 863
HpaI GTTAAC 1 cut(s) 594
HpaII CCGG 4 cut(s) 143, 325, 358, 458
HphI GGTGA 2 cut(s) 154, 753
Hpy166II GTNNAC 4 cut(s) 259, 484, 594, 630
Hpy188I TCNGA 2 cut(s) 311, 868
Hpy188III TCNNGA 6 cut(s) 70, 116, 379, 458, 506, 613
Hpy8I GTNNAC 4 cut(s) 259, 484, 594, 630
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 328, 400
HpyCH4III ACNGT 5 cut(s) 315, 634, 671, 821, 881
HpyCH4IV ACGT 3 cut(s) 32, 267, 331
HpyCH4V TGCA 2 cut(s) 4, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 354, 646
HpyF3I CTNAG 1 cut(s) 715
HpySE526I ACGT 3 cut(s) 32, 267, 331
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 8 cut(s) 8, 66, 124, 535, 563, 590, 616, 899
HspAI GCGC 2 cut(s) 253, 283
Kpn2I TCCGGA 1 cut(s) 457
KspAI GTTAAC 1 cut(s) 594
Kzo9I GATC 2 cut(s) 306, 487
LguI GCTCTTC 1 cut(s) 732
LmnI GCTCC 3 cut(s) 296, 367, 695
LweI GCATC 1 cut(s) 675
MaeI CTAG 1 cut(s) 857
MaeII ACGT 3 cut(s) 32, 267, 331
MaeIII GTNAC 2 cut(s) 92, 881
MalI GATC 2 cut(s) 308, 489
MbiI CCGCTC 2 cut(s) 160, 291
MboI GATC 2 cut(s) 306, 487
MhlI GDGCHC 4 cut(s) 140, 202, 651, 796
MluCI AATT 6 cut(s) 382, 576, 641, 674, 689, 925
MlyI GAGTC 3 cut(s) 227, 476, 857
MmeI TCCRAC 2 cut(s) 82, 261
MnlI CCTC 7 cut(s) 343, 388, 417, 454, 513, 620, 727
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 620
MseI TTAA 3 cut(s) 414, 567, 593
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 4 cut(s) 143, 325, 358, 458
MspR9I CCNGG 5 cut(s) 143, 176, 182, 325, 829
MvaI CCWGG 3 cut(s) 176, 182, 829
MvnI CGCG 5 cut(s) 194, 213, 255, 348, 601
MwoI GCNNNNNNNGC 3 cut(s) 191, 354, 646
NciI CCSGG 2 cut(s) 143, 325
NdeII GATC 2 cut(s) 306, 487
NheI GCTAGC 1 cut(s) 856
NlaIII CATG 8 cut(s) 8, 66, 124, 535, 563, 590, 616, 899
NlaIV GGNNCC 2 cut(s) 199, 200
NmuCI GTSAC 2 cut(s) 92, 881
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 612
PciSI GCTCTTC 1 cut(s) 732
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 222, 345
PdmI GAANNNNTTC 1 cut(s) 620
PfeI GAWTC 4 cut(s) 101, 441, 509, 839
PfoI TCCNGGA 1 cut(s) 323
PkrI GCNGC 3 cut(s) 127, 212, 475
Ple19I CGATCG 1 cut(s) 490
PleI GAGTC 3 cut(s) 227, 475, 857
PpsI GAGTC 3 cut(s) 227, 475, 857
Psp6I CCWGG 3 cut(s) 174, 180, 827
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 3 cut(s) 174, 180, 827
PspN4I GGNNCC 2 cut(s) 199, 200
PspOMI GGGCCC 1 cut(s) 198
PspPI GGNCC 6 cut(s) 172, 178, 184, 198, 199, 315
PstNI CAGNNNCTG 1 cut(s) 175
PvuI CGATCG 1 cut(s) 490
RseI CAYNNNNRTG 1 cut(s) 530
SapI GCTCTTC 1 cut(s) 732
SaqAI TTAA 3 cut(s) 414, 567, 593
SatI GCNGC 3 cut(s) 126, 211, 474
Sau3AI GATC 2 cut(s) 306, 487
Sau96I GGNCC 6 cut(s) 172, 178, 184, 198, 199, 315
SchI GAGTC 3 cut(s) 227, 476, 857
ScrFI CCNGG 5 cut(s) 143, 176, 182, 325, 829
SduI GDGCHC 4 cut(s) 140, 202, 651, 796
SfaNI GCATC 1 cut(s) 675
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 530
Sse9I AATT 6 cut(s) 382, 576, 641, 674, 689, 925
SspMI CTAG 1 cut(s) 857
StyD4I CCNGG 5 cut(s) 141, 174, 180, 323, 827
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 5 cut(s) 315, 634, 671, 821, 881
TaiI ACGT 3 cut(s) 35, 270, 334
TaqI TCGA 2 cut(s) 645, 679
TasI AATT 6 cut(s) 382, 576, 641, 674, 689, 925
TauI GCSGC 3 cut(s) 128, 213, 476
TfiI GAWTC 4 cut(s) 101, 441, 509, 839
Tru1I TTAA 3 cut(s) 414, 567, 593
Tru9I TTAA 3 cut(s) 414, 567, 593
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 2 cut(s) 92, 881
Tsp45I GTSAC 2 cut(s) 92, 881
TspDTI ATGAA 6 cut(s) 419, 548, 629, 823, 831, 912
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 1 cut(s) 886
VpaK11BI GGWCC 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 179
XapI RAATTY 2 cut(s) 382, 674
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 1 cut(s) 857
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.