FvH4_6g05240

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010048
Physical Location & Seq
Forward (+)
2959714 .. 2962345
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05240.t1

Sequence Viewer

Length: 951 bp
ATGCATCCGATTCATAGAAATGGGAGACCTTCATCTTCATCGTCTTCACCATCATCATCATCATCTCCCTCTTCCTCACAGCCATGTCTCCCCAAACGCATAATCCTAATGCGCCACGCCGAATCCCAAGGCAATCTCGACACGGCGGCCTACACCACAACCCCGGACAACAAAATCCCTCTCACCGAACAGGGCCTGGCCCAGGCCGAGCTCGCCGGGGCCCAGCTCCGCCGCGTCATCTCCGCCGACAACGACTCCAACCACCCCGAGTGGCGCGTCTGCTTCTACGTCTCCCCCTACGAGCGCACGCGGTCGACGCTGTCACAGATCGGACGGTCGTTCTCGCGGAAACGTGTCATCGGCGTCAGGGAGGAGTGCCGGATAAGGGAGCAGGATTTCGGGAATTTCCAGGTGCAGGAGAGAATGACGGCGATTAAGCAGACCAGGGAGAAGTTTGGAAGATTCTTTTATAGGTTTCCGGAAGGGGAGTCCGCCGCTGATGTTTACGATCGCGTTTCAAGTTTTCTTGAATCTTTATGGAGGGACATAGACATGAACAGGCTTCGCCACAACCCTTGCCATGACCTCAATCTTGTAATTGTATCACATGGGTTAACATCGCGTGTCTTCCTCATGAAGTGGTTCAAGTGGTCTGTAGAGCAATTTGAGCACCTAAACAATTTTGGAAACTGTGAATTTCGAGTAATGCAATTAGGAGCTGGCGGAGAGTACAGCTTAGCAGTCCATCACACTGAGGAAGAGCTAACAGAATGGGGTCTTTCACCTGAAATGATAGCTGACCAAAAGTGGCGAGCCCGTTCTGGCAAGGGTTGGACTGACCGTTGCCCGTGGTACCTTGATTCATTCTTTGACAAGCTAGCGGACTCGGATGACACAGACAATGACGATGTTTGGCATGAAACTAACGATAAAGTTCAGCAATCAAATTGA

Protein Analysis

317

Amino Acids

36.5

Weight (kDa)

6.14

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 34 - 243 7.9e-27 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 852
AccB1I GGYRCC 1 cut(s) 852
AccI GTMKAC 1 cut(s) 314
AccII CGCG 6 cut(s) 234, 276, 310, 346, 513, 622
AccIII TCCGGA 1 cut(s) 478
AcsI RAATTY 2 cut(s) 403, 695
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 2 cut(s) 731, 854
AfiI CCNNNNNNNGG 3 cut(s) 202, 385, 415
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 3 cut(s) 519, 530, 646
AjnI CCWGG 4 cut(s) 195, 201, 408, 443
AluBI AGCT 7 cut(s) 211, 226, 719, 735, 763, 797, 877
AluI AGCT 7 cut(s) 211, 226, 719, 735, 763, 797, 877
Alw21I GWGCWC 2 cut(s) 213, 672
Alw26I GTCTC 3 cut(s) 19, 92, 295
AlwNI CAGNNNCTG 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 5 cut(s) 147, 193, 198, 204, 219
ApaI GGGCCC 1 cut(s) 223
ApoI RAATTY 2 cut(s) 403, 695
Asp700I GAANNNNTTC 1 cut(s) 641
Asp718I GGTACC 1 cut(s) 852
AspLEI GCGC 3 cut(s) 114, 276, 306
AspS9I GGNCC 4 cut(s) 193, 199, 219, 220
AsuC2I CCSGG 2 cut(s) 164, 217
AsuHPI GGTGA 3 cut(s) 39, 175, 774
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 852
BanII GRGCYC 3 cut(s) 213, 223, 817
BbsI GAAGAC 2 cut(s) 36, 619
Bbv12I GWGCWC 2 cut(s) 213, 672
BccI CCATC 2 cut(s) 58, 753
BceAI ACGGC 2 cut(s) 159, 444
BciT130I CCWGG 4 cut(s) 197, 203, 410, 445
BcnI CCSGG 2 cut(s) 164, 217
BcoDI GTCTC 3 cut(s) 19, 92, 295
BfaI CTAG 1 cut(s) 878
BfmI CTRYAG 1 cut(s) 654
BisI GCNGC 3 cut(s) 147, 232, 495
BlpI GCTNAGC 1 cut(s) 736
BlsI GCNGC 3 cut(s) 148, 233, 496
Bme1390I CCNGG 6 cut(s) 164, 197, 203, 217, 410, 445
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 4 cut(s) 193, 199, 219, 220
BmiI GGNNCC 3 cut(s) 220, 221, 854
BmrFI CCNGG 6 cut(s) 164, 197, 203, 217, 410, 445
BmsI GCATC 1 cut(s) 13
BmtI GCTAGC 1 cut(s) 881
BpiI GAAGAC 2 cut(s) 36, 619
Bpu1102I GCTNAGC 1 cut(s) 736
BpuMI CCSGG 2 cut(s) 164, 217
BsaHI GRCGYC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 6 cut(s) 127, 162, 201, 216, 444, 848
BsaWI WCCGGW 1 cut(s) 478
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 385, 415
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 4 cut(s) 197, 203, 410, 445
BseDI CCNNGG 6 cut(s) 127, 162, 201, 216, 444, 848
BseGI GGATG 2 cut(s) 4, 895
BseLI CCNNNNNNNGG 3 cut(s) 202, 385, 415
BseMII CTCAG 1 cut(s) 744
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 223
BseYI CCCAGC 1 cut(s) 222
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 6 cut(s) 234, 276, 310, 346, 513, 622
Bsh1285I CGRYCG 3 cut(s) 314, 338, 511
BshFI GGCC 5 cut(s) 149, 195, 200, 206, 221
BshNI GGYRCC 1 cut(s) 852
BsiEI CGRYCG 3 cut(s) 314, 338, 511
BsiHKAI GWGCWC 2 cut(s) 213, 672
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 4 cut(s) 164, 216, 379, 479
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 3 cut(s) 202, 385, 415
BsmAI GTCTC 3 cut(s) 19, 92, 295
BsmBI CGTCTC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 5 cut(s) 149, 195, 200, 206, 221
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 266
Bsp120I GGGCCC 1 cut(s) 219
Bsp1286I GDGCHC 4 cut(s) 213, 223, 672, 817
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 2 cut(s) 327, 508
Bsp1720I GCTNAGC 1 cut(s) 736
BspANI GGCC 5 cut(s) 149, 195, 200, 206, 221
BspCNI CTCAG 1 cut(s) 745
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 6 cut(s) 234, 276, 310, 346, 513, 622
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 3 cut(s) 220, 221, 854
BspOI GCTAGC 1 cut(s) 881
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 1 cut(s) 852
BspTNI GGTCTC 1 cut(s) 19
BssECI CCNNGG 6 cut(s) 127, 162, 201, 216, 444, 848
BssMI GATC 2 cut(s) 327, 508
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 4 cut(s) 197, 203, 410, 445
Bst4CI ACNGT 3 cut(s) 336, 692, 842
Bst6I CTCTTC 2 cut(s) 76, 753
BstACI GRCGYC 1 cut(s) 363
BstC8I GCNNGC 5 cut(s) 213, 308, 721, 813, 879
BstDEI CTNAG 2 cut(s) 736, 753
BstDSI CCRYGG 1 cut(s) 848
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 4, 895
BstFNI CGCG 6 cut(s) 234, 276, 310, 346, 513, 622
BstHHI GCGC 3 cut(s) 114, 276, 306
BstKTI GATC 2 cut(s) 330, 511
BstMAI GTCTC 3 cut(s) 19, 92, 295
BstMBI GATC 2 cut(s) 327, 508
BstMCI CGRYCG 3 cut(s) 314, 338, 511
BstMWI GCNNNNNNNGC 3 cut(s) 212, 316, 667
BstNI CCWGG 4 cut(s) 197, 203, 410, 445
BstSCI CCNGG 6 cut(s) 162, 195, 201, 215, 408, 443
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 6 cut(s) 234, 276, 310, 346, 513, 622
BstV2I GAAGAC 2 cut(s) 36, 619
BsuRI GGCC 5 cut(s) 149, 195, 200, 206, 221
BtgI CCRYGG 1 cut(s) 848
BtgZI GCGATG 1 cut(s) 603
BtsCI GGATG 2 cut(s) 4, 895
BtsIMutI CAGTG 1 cut(s) 750
Cac8I GCNNGC 5 cut(s) 213, 308, 721, 813, 879
CaiI CAGNNNCTG 1 cut(s) 196
CciI TCATGA 1 cut(s) 633
CfoI GCGC 3 cut(s) 114, 276, 306
Cfr13I GGNCC 4 cut(s) 193, 199, 219, 220
CseI GACGC 4 cut(s) 223, 265, 325, 352
Csp6I GTAC 2 cut(s) 730, 853
CspCI CAANNNNNGTGG 2 cut(s) 557, 592
CviAII CATG 6 cut(s) 84, 553, 581, 608, 634, 917
CviQI GTAC 2 cut(s) 730, 853
DdeI CTNAG 2 cut(s) 736, 753
DpnI GATC 2 cut(s) 329, 510
DpnII GATC 2 cut(s) 327, 508
Eam1104I CTCTTC 2 cut(s) 76, 753
EarI CTCTTC 2 cut(s) 76, 753
EciI GGCGGA 4 cut(s) 218, 232, 481, 738
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 3 cut(s) 213, 223, 817
Eco31I GGTCTC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 211
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 193, 219
EcoRII CCWGG 4 cut(s) 195, 201, 408, 443
EcoT14I CCWWGG 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 213, 223, 817
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 6 cut(s) 87, 556, 584, 611, 637, 920
FaqI GGGAC 1 cut(s) 557
FatI CATG 6 cut(s) 83, 552, 580, 607, 633, 916
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 3 cut(s) 147, 232, 495
FokI GGATG 1 cut(s) 902
FriOI GRGCYC 3 cut(s) 213, 223, 817
Fsp4HI GCNGC 3 cut(s) 147, 232, 495
FspBI CTAG 1 cut(s) 878
GlaI GCGC 3 cut(s) 113, 275, 305
GluI GCNGC 3 cut(s) 147, 232, 495
GsaI CCCAGC 1 cut(s) 226
HaeIII GGCC 5 cut(s) 149, 195, 200, 206, 221
HapII CCGG 4 cut(s) 164, 216, 379, 479
HgaI GACGC 4 cut(s) 223, 265, 325, 352
HhaI GCGC 3 cut(s) 114, 276, 306
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 6 cut(s) 87, 556, 584, 611, 637, 920
Hin6I GCGC 3 cut(s) 112, 274, 304
HinP1I GCGC 3 cut(s) 112, 274, 304
HincII GTYRAC 2 cut(s) 315, 615
HindII GTYRAC 2 cut(s) 315, 615
HinfI GANTC 8 cut(s) 10, 122, 254, 462, 488, 530, 860, 884
HpaI GTTAAC 1 cut(s) 615
HpaII CCGG 4 cut(s) 164, 216, 379, 479
HphI GGTGA 3 cut(s) 39, 175, 774
Hpy166II GTNNAC 3 cut(s) 315, 505, 615
Hpy188I TCNGA 3 cut(s) 9, 332, 889
Hpy188III TCNNGA 5 cut(s) 137, 400, 479, 527, 634
Hpy8I GTNNAC 3 cut(s) 315, 505, 615
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 2 cut(s) 39, 476
HpyCH4III ACNGT 3 cut(s) 336, 692, 842
HpyCH4IV ACGT 2 cut(s) 288, 352
HpyCH4V TGCA 3 cut(s) 4, 415, 709
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 316, 667
HpyF3I CTNAG 2 cut(s) 736, 753
HpySE526I ACGT 2 cut(s) 288, 352
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 6 cut(s) 87, 556, 584, 611, 637, 920
HspAI GCGC 3 cut(s) 112, 274, 304
Kpn2I TCCGGA 1 cut(s) 478
KpnI GGTACC 1 cut(s) 856
KspAI GTTAAC 1 cut(s) 615
Kzo9I GATC 2 cut(s) 327, 508
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 3 cut(s) 231, 388, 716
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 878
MaeII ACGT 2 cut(s) 288, 352
MaeIII GTNAC 1 cut(s) 321
MalI GATC 2 cut(s) 329, 510
MboI GATC 2 cut(s) 327, 508
MboII GAAGA 6 cut(s) 27, 36, 63, 471, 619, 770
MhlI GDGCHC 4 cut(s) 213, 223, 672, 817
MluCI AATT 7 cut(s) 403, 597, 662, 679, 695, 710, 946
MlyI GAGTC 3 cut(s) 248, 497, 878
MmeI TCCRAC 2 cut(s) 282, 812
MnlI CCTC 8 cut(s) 79, 85, 189, 364, 534, 596, 641, 748
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 2 cut(s) 435, 614
MslI CAYNNNNRTG 3 cut(s) 18, 82, 551
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 4 cut(s) 164, 216, 379, 479
MspR9I CCNGG 6 cut(s) 164, 197, 203, 217, 410, 445
MvaI CCWGG 4 cut(s) 197, 203, 410, 445
MvnI CGCG 6 cut(s) 234, 276, 310, 346, 513, 622
MwoI GCNNNNNNNGC 3 cut(s) 212, 316, 667
NciI CCSGG 2 cut(s) 164, 217
NdeII GATC 2 cut(s) 327, 508
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 6 cut(s) 87, 556, 584, 611, 637, 920
NlaIV GGNNCC 3 cut(s) 220, 221, 854
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 633
PciSI GCTCTTC 1 cut(s) 753
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 5 cut(s) 10, 122, 462, 530, 860
PflFI GACNNNGTC 1 cut(s) 319
PkrI GCNGC 3 cut(s) 148, 233, 496
Ple19I CGATCG 1 cut(s) 511
PleI GAGTC 3 cut(s) 248, 496, 878
PpsI GAGTC 3 cut(s) 248, 496, 878
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 4 cut(s) 195, 201, 408, 443
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 4 cut(s) 195, 201, 408, 443
PspN4I GGNNCC 3 cut(s) 220, 221, 854
PspOMI GGGCCC 1 cut(s) 219
PspPI GGNCC 4 cut(s) 193, 199, 219, 220
PstNI CAGNNNCTG 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 319
PvuI CGATCG 1 cut(s) 511
RsaI GTAC 2 cut(s) 731, 854
RsaNI GTAC 2 cut(s) 730, 853
RseI CAYNNNNRTG 3 cut(s) 18, 82, 551
SacI GAGCTC 1 cut(s) 213
SalI GTCGAC 1 cut(s) 313
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 2 cut(s) 435, 614
SatI GCNGC 3 cut(s) 147, 232, 495
Sau3AI GATC 2 cut(s) 327, 508
Sau96I GGNCC 4 cut(s) 193, 199, 219, 220
SchI GAGTC 3 cut(s) 248, 497, 878
ScrFI CCNGG 6 cut(s) 164, 197, 203, 217, 410, 445
SduI GDGCHC 4 cut(s) 213, 223, 672, 817
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 654
SmiMI CAYNNNNRTG 3 cut(s) 18, 82, 551
Sse9I AATT 7 cut(s) 403, 597, 662, 679, 695, 710, 946
SspMI CTAG 1 cut(s) 878
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 6 cut(s) 162, 195, 201, 215, 408, 443
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 3 cut(s) 336, 692, 842
TaiI ACGT 2 cut(s) 291, 355
TaqI TCGA 3 cut(s) 138, 314, 700
TasI AATT 7 cut(s) 403, 597, 662, 679, 695, 710, 946
TatI WGTACW 1 cut(s) 729
TauI GCSGC 3 cut(s) 149, 234, 497
TfiI GAWTC 5 cut(s) 10, 122, 462, 530, 860
Tru1I TTAA 2 cut(s) 435, 614
Tru9I TTAA 2 cut(s) 435, 614
TscAI CASTG 1 cut(s) 757
TseFI GTSAC 1 cut(s) 321
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 6 cut(s) 21, 27, 569, 650, 852, 933
TspRI CASTG 1 cut(s) 757
Tth111I GACNNNGTC 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 2 cut(s) 403, 695
XmiI GTMKAC 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 1 cut(s) 878
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.