RchiOBHm_Chr3g0453971

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010048
Physical Location & Seq
Forward (+)
4229916 .. 4232105
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42100

Sequence Viewer

Length: 921 bp
ATGCATGGGAGACCATCTTCTTCGTCTTCTACGTCTTCTTCTTCTCTCCAACCATGTCTCCCGAAACGGATAATCCTAATGCGTCACGCCGAATCCCAAGGCAATCTGGACATGGCGGCCTACACGAGCACCCCGGACAACAAAATCCCGCTCACCGAACAGGGCCTGGCCCAGGCCCAGCTCGCGGGGGCCCAACTCCGCCGCGTCGTCTCCGCCGACAACGACTCCAACCACCCCGAGTGGCGCGTCTACTTCTACGTCTCCCCTTACGAGCGCACCCGCTCCACGCTCTCCCAGATCGGACGGTCCTTCTCCCGGAAACGTGTCATCGGCGTCCGAGAGGAGTGCCGGATAAGGGAGCAGGATTTCGGGAATTTTCAGGTGGAGGAGAGAATGAAGGCCATTAAGCAGACGAGGCAGAAGTTCGGCAGATTCTTCTATCGGTTTCCGGAGGGGGAGTCCGCCGCTGATGTTTACGATCGTGTTTCCAGTTTTCTTGAATCTTTATGGAGGGACATAGACATGAACAGGCTTCGCCACAATCCTTGCCATGACCTTAATCTTGTAATTGTATCACATGGGTTAACATCGCGTGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTAGAGCAATTCGAGCACCTAAACAACTTTGGAAACTGTGAATTTCGAGTGATGCAATTAGGAGCTTGCGGAGAGTATAGCTTAGCAGTCCATCACACAGAGGAAGAGCTAATAGAATGGGGTCTTTCACGTGAAATGATAGATGACCAAAAGTGGCGAGCGCATTCGGGCAAGGGTGGGACTGACCGTTGCCCCTGGTTCGTTGATTCATTCTTTGACAAGCTAGCCGACTCTGACGACACAGACAGTGACGATGTTTGGCATGAAACCAATGACAAAGTTCAGCAATCAAATTGA

Protein Analysis

306

Amino Acids

35.53

Weight (kDa)

6.14

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 24 - 232 6e-26 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 151, 282
AccI GTMKAC 1 cut(s) 249
AccII CGCG 4 cut(s) 185, 204, 246, 592
AccIII TCCGGA 1 cut(s) 448
AcsI RAATTY 2 cut(s) 373, 665
AcvI CACGTG 1 cut(s) 755
AcyI GRCGYC 1 cut(s) 333
AfiI CCNNNNNNNGG 4 cut(s) 172, 184, 315, 355
AflIII ACRYGT 1 cut(s) 322
AgsI TTSAA 2 cut(s) 500, 616
AjnI CCWGG 3 cut(s) 165, 171, 818
AluBI AGCT 5 cut(s) 181, 689, 705, 733, 847
AluI AGCT 5 cut(s) 181, 689, 705, 733, 847
Alw21I GWGCWC 2 cut(s) 131, 642
Alw26I GTCTC 4 cut(s) 4, 62, 214, 265
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 236
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 6 cut(s) 117, 163, 168, 174, 189, 399
ApaI GGGCCC 1 cut(s) 193
ApoI RAATTY 2 cut(s) 373, 665
Asp700I GAANNNNTTC 1 cut(s) 611
AspLEI GCGC 3 cut(s) 246, 276, 787
AspS9I GGNCC 6 cut(s) 163, 169, 175, 189, 190, 306
AsuC2I CCSGG 2 cut(s) 134, 316
AsuHPI GGTGA 1 cut(s) 145
AsuNHI GCTAGC 1 cut(s) 847
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 193
BauI CACGAG 1 cut(s) 124
BbrPI CACGTG 1 cut(s) 755
BbsI GAAGAC 3 cut(s) 18, 27, 589
Bbv12I GWGCWC 2 cut(s) 131, 642
BccI CCATC 2 cut(s) 22, 723
BcgI CGANNNNNNTGC 2 cut(s) 327, 361
BciT130I CCWGG 3 cut(s) 167, 173, 820
BcnI CCSGG 2 cut(s) 134, 316
BcoDI GTCTC 4 cut(s) 4, 62, 214, 265
BfaI CTAG 1 cut(s) 848
BisI GCNGC 3 cut(s) 117, 202, 465
BlpI GCTNAGC 1 cut(s) 706
BlsI GCNGC 3 cut(s) 118, 203, 466
Bme1390I CCNGG 5 cut(s) 134, 167, 173, 316, 820
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 6 cut(s) 163, 169, 175, 189, 190, 306
BmiI GGNNCC 2 cut(s) 190, 191
BmrFI CCNGG 5 cut(s) 134, 167, 173, 316, 820
BmsI GCATC 1 cut(s) 666
BmtI GCTAGC 1 cut(s) 851
BpiI GAAGAC 3 cut(s) 18, 27, 589
Bpu1102I GCTNAGC 1 cut(s) 706
BpuMI CCSGG 2 cut(s) 134, 316
BsaAI YACGTR 1 cut(s) 755
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 4 cut(s) 97, 132, 171, 818
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 6 cut(s) 42, 72, 209, 239, 443, 473
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 184, 315, 355
Bse1I ACTGG 1 cut(s) 489
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 3 cut(s) 167, 173, 820
BseDI CCNNGG 4 cut(s) 97, 132, 171, 818
BseLI CCNNNNNNNGG 4 cut(s) 172, 184, 315, 355
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 356, 401
BseSI GKGCMC 1 cut(s) 193
BseYI CCCAGC 1 cut(s) 177
Bsh1236I CGCG 4 cut(s) 185, 204, 246, 592
Bsh1285I CGRYCG 1 cut(s) 481
BshFI GGCC 6 cut(s) 119, 165, 170, 176, 191, 401
BsiEI CGRYCG 1 cut(s) 481
BsiHKAI GWGCWC 2 cut(s) 131, 642
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 4 cut(s) 134, 316, 349, 449
BslFI GGGAC 2 cut(s) 527, 817
BslI CCNNNNNNNGG 4 cut(s) 172, 184, 315, 355
BsmAI GTCTC 4 cut(s) 4, 62, 214, 265
BsmBI CGTCTC 2 cut(s) 214, 265
BsmFI GGGAC 2 cut(s) 527, 817
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 6 cut(s) 119, 165, 170, 176, 191, 401
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 236
Bsp120I GGGCCC 1 cut(s) 189
Bsp1286I GDGCHC 3 cut(s) 131, 193, 642
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 297, 478
Bsp1720I GCTNAGC 1 cut(s) 706
BspANI GGCC 6 cut(s) 119, 165, 170, 176, 191, 401
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 4 cut(s) 185, 204, 246, 592
BspHI TCATGA 1 cut(s) 603
BspLI GGNNCC 2 cut(s) 190, 191
BspOI GCTAGC 1 cut(s) 851
BspQI GCTCTTC 1 cut(s) 723
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 2 cut(s) 151, 282
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 4 cut(s) 97, 132, 171, 818
BssMI GATC 2 cut(s) 297, 478
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 167, 173, 820
Bst4CI ACNGT 5 cut(s) 306, 625, 662, 812, 872
Bst6I CTCTTC 1 cut(s) 723
BstACI GRCGYC 1 cut(s) 333
BstBAI YACGTR 1 cut(s) 755
BstC8I GCNNGC 4 cut(s) 183, 691, 783, 849
BstDEI CTNAG 1 cut(s) 706
BstENI CCTNNNNNAGG 1 cut(s) 170
BstFNI CGCG 4 cut(s) 185, 204, 246, 592
BstHHI GCGC 3 cut(s) 246, 276, 787
BstKTI GATC 2 cut(s) 300, 481
BstMAI GTCTC 4 cut(s) 4, 62, 214, 265
BstMBI GATC 2 cut(s) 297, 478
BstMCI CGRYCG 1 cut(s) 481
BstMWI GCNNNNNNNGC 3 cut(s) 182, 415, 637
BstNI CCWGG 3 cut(s) 167, 173, 820
BstSCI CCNGG 5 cut(s) 132, 165, 171, 314, 818
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 4 cut(s) 185, 204, 246, 592
BstV2I GAAGAC 3 cut(s) 18, 27, 589
BsuRI GGCC 6 cut(s) 119, 165, 170, 176, 191, 401
BtgZI GCGATG 1 cut(s) 573
BtsIMutI CAGTG 1 cut(s) 877
Cac8I GCNNGC 4 cut(s) 183, 691, 783, 849
CaiI CAGNNNCTG 1 cut(s) 166
CciI TCATGA 1 cut(s) 603
CfoI GCGC 3 cut(s) 246, 276, 787
Cfr13I GGNCC 6 cut(s) 163, 169, 175, 189, 190, 306
CseI GACGC 4 cut(s) 71, 193, 235, 322
CspCI CAANNNNNGTGG 2 cut(s) 527, 562
CviAII CATG 8 cut(s) 5, 54, 112, 523, 551, 578, 604, 887
DdeI CTNAG 1 cut(s) 706
DpnI GATC 2 cut(s) 299, 480
DpnII GATC 2 cut(s) 297, 478
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
EciI GGCGGA 3 cut(s) 188, 202, 451
Eco130I CCWWGG 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 306
Eco72I CACGTG 1 cut(s) 755
Eco88I CYCGRG 1 cut(s) 236
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoO109I RGGNCCY 2 cut(s) 163, 189
EcoRII CCWGG 3 cut(s) 165, 171, 818
EcoT14I CCWWGG 1 cut(s) 97
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 97
Esp3I CGTCTC 2 cut(s) 214, 265
FaeI CATG 8 cut(s) 8, 57, 115, 526, 554, 581, 607, 890
FaqI GGGAC 2 cut(s) 527, 817
FatI CATG 8 cut(s) 4, 53, 111, 522, 550, 577, 603, 886
FauI CCCGC 3 cut(s) 156, 178, 287
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 3 cut(s) 117, 202, 465
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 3 cut(s) 117, 202, 465
FspBI CTAG 1 cut(s) 848
GlaI GCGC 3 cut(s) 245, 275, 786
GluI GCNGC 3 cut(s) 117, 202, 465
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 6 cut(s) 119, 165, 170, 176, 191, 401
HapII CCGG 4 cut(s) 134, 316, 349, 449
HgaI GACGC 4 cut(s) 71, 193, 235, 322
HhaI GCGC 3 cut(s) 246, 276, 787
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 8 cut(s) 8, 57, 115, 526, 554, 581, 607, 890
Hin6I GCGC 3 cut(s) 244, 274, 785
HinP1I GCGC 3 cut(s) 244, 274, 785
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HinfI GANTC 7 cut(s) 92, 224, 432, 458, 500, 830, 854
HpaI GTTAAC 1 cut(s) 585
HpaII CCGG 4 cut(s) 134, 316, 349, 449
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 4 cut(s) 250, 475, 585, 621
Hpy188I TCNGA 3 cut(s) 302, 338, 859
Hpy188III TCNNGA 6 cut(s) 61, 107, 370, 449, 497, 604
Hpy8I GTNNAC 4 cut(s) 250, 475, 585, 621
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 319, 391
HpyCH4III ACNGT 5 cut(s) 306, 625, 662, 812, 872
HpyCH4IV ACGT 4 cut(s) 32, 258, 322, 754
HpyCH4V TGCA 2 cut(s) 4, 679
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 415, 637
HpyF3I CTNAG 1 cut(s) 706
HpySE526I ACGT 4 cut(s) 32, 258, 322, 754
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 8 cut(s) 8, 57, 115, 526, 554, 581, 607, 890
HspAI GCGC 3 cut(s) 244, 274, 785
Kpn2I TCCGGA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 585
Kzo9I GATC 2 cut(s) 297, 478
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 3 cut(s) 287, 358, 686
LweI GCATC 1 cut(s) 666
MaeI CTAG 1 cut(s) 848
MaeII ACGT 4 cut(s) 32, 258, 322, 754
MaeIII GTNAC 2 cut(s) 83, 872
MalI GATC 2 cut(s) 299, 480
MbiI CCGCTC 2 cut(s) 151, 282
MboI GATC 2 cut(s) 297, 478
MboII GAAGA 9 cut(s) 9, 12, 18, 27, 30, 33, 427, 589, 740
MhlI GDGCHC 3 cut(s) 131, 193, 642
MluCI AATT 6 cut(s) 373, 567, 632, 665, 680, 916
MlyI GAGTC 3 cut(s) 218, 467, 848
MmeI TCCRAC 2 cut(s) 73, 252
MnlI CCTC 7 cut(s) 334, 379, 408, 445, 504, 611, 718
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 448
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 3 cut(s) 405, 558, 584
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 4 cut(s) 134, 316, 349, 449
MspR9I CCNGG 5 cut(s) 134, 167, 173, 316, 820
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 3 cut(s) 167, 173, 820
MvnI CGCG 4 cut(s) 185, 204, 246, 592
MwoI GCNNNNNNNGC 3 cut(s) 182, 415, 637
NciI CCSGG 2 cut(s) 134, 316
NdeII GATC 2 cut(s) 297, 478
NheI GCTAGC 1 cut(s) 847
NlaIII CATG 8 cut(s) 8, 57, 115, 526, 554, 581, 607, 890
NlaIV GGNNCC 2 cut(s) 190, 191
NmuCI GTSAC 2 cut(s) 83, 872
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 603
PciSI GCTCTTC 1 cut(s) 723
PcsI WCGNNNNNNNCGW 2 cut(s) 29, 213
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 4 cut(s) 92, 432, 500, 830
PfoI TCCNGGA 1 cut(s) 314
PkrI GCNGC 3 cut(s) 118, 203, 466
Ple19I CGATCG 1 cut(s) 481
PleI GAGTC 3 cut(s) 218, 466, 848
PmaCI CACGTG 1 cut(s) 755
PmlI CACGTG 1 cut(s) 755
PpsI GAGTC 3 cut(s) 218, 466, 848
Ppu21I YACGTR 1 cut(s) 755
Psp6I CCWGG 3 cut(s) 165, 171, 818
PspCI CACGTG 1 cut(s) 755
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 3 cut(s) 165, 171, 818
PspN4I GGNNCC 2 cut(s) 190, 191
PspOMI GGGCCC 1 cut(s) 189
PspPI GGNCC 6 cut(s) 163, 169, 175, 189, 190, 306
PstNI CAGNNNCTG 1 cut(s) 166
PvuI CGATCG 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 3 cut(s) 405, 558, 584
SatI GCNGC 3 cut(s) 117, 202, 465
Sau3AI GATC 2 cut(s) 297, 478
Sau96I GGNCC 6 cut(s) 163, 169, 175, 189, 190, 306
SchI GAGTC 3 cut(s) 218, 467, 848
ScrFI CCNGG 5 cut(s) 134, 167, 173, 316, 820
SduI GDGCHC 3 cut(s) 131, 193, 642
SfaNI GCATC 1 cut(s) 666
SinI GGWCC 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 521
Sse9I AATT 6 cut(s) 373, 567, 632, 665, 680, 916
SspMI CTAG 1 cut(s) 848
StyD4I CCNGG 5 cut(s) 132, 165, 171, 314, 818
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 5 cut(s) 306, 625, 662, 812, 872
TaiI ACGT 4 cut(s) 35, 261, 325, 757
TaqI TCGA 2 cut(s) 636, 670
TasI AATT 6 cut(s) 373, 567, 632, 665, 680, 916
TauI GCSGC 3 cut(s) 119, 204, 467
TfiI GAWTC 4 cut(s) 92, 432, 500, 830
Tru1I TTAA 3 cut(s) 405, 558, 584
Tru9I TTAA 3 cut(s) 405, 558, 584
TscAI CASTG 1 cut(s) 877
TseFI GTSAC 2 cut(s) 83, 872
Tsp45I GTSAC 2 cut(s) 83, 872
TspDTI ATGAA 5 cut(s) 410, 539, 620, 822, 903
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 1 cut(s) 877
VpaK11BI GGWCC 1 cut(s) 306
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 2 cut(s) 373, 665
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 1 cut(s) 848
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.