Rh3BG056200

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010048
Physical Location & Seq
Forward (+)
3865610 .. 3867843
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG056200.1

Sequence Viewer

Length: 927 bp
ATGCATGGGAGACCATCTTCTTCGTCTTCTACGTCTTCGTCTTCTTCTTCTCTCCAACCATGTCTCCCGAAACGGATAATCCTAATGCGTCACGCCGAATCCCAAGGCAATCTGGACATGGCGGCCTACACGACCACCCCGGACAACAAAATCCCGCTCACCGAACAGGGCTTGGCCCAGGCCCAGCTCGCGGGGGCCCAACTCCGCCGCGTCGTCTCCGCCGACAACGACTCCAACCACCCCGAGTGGCGCGTCTACTTCTACGTCTCCCCTTACGAACGCACCCGCTCCACGCTCTCCCAGATCGGACGGTCCTTCTCCCGGACGCGTGTCATCGGCGTCCGCGAGGAGTGCCGGATAAGGGAGCAGGATTTCGGGAATTTTCAGGTGGAGGAGAGAATGAAGGCCATTAAGCAGACGAGGGAGAAGTTCGGCAGATTCTTCTATCGGTTTCCGGAGGGGGAGTCCGCCGCTGATGTTTACGATCGTGTTTCCAGTTTTCTTGAATCTTTATGGAGGGACATAGACATGAACAGGCTTCGCCACAACCCTTGCCATGACCTTAATCTTGTAATTGTATCACATGGGTTAACATCGCGTGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTAGAGCAATTCGAGCACCTAAACAACTTTGGAAACTGTGAATTTCGAGTGATGCAATTAGGAGCTGGCGGAGAGTATAGCTTAGCAGTCCATCACACAGAGAAAGAGCTAATAGAATGGGGTCTTTCACCTGAAATGATAGCTGACCAAAAGTGGCGAGCCCATTCGGGCAAGGGTTGGACTGACCGTTGCCCCTGGTTCATTGATTCATTCTTTGACAAGCTAGCCGACTCTGATGACACAGACAGTGACGATGTTTGGCATGAAACCAATGAAAAAGTTCAGCAATCAAATTGA

Protein Analysis

308

Amino Acids

35.7

Weight (kDa)

6.14

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 26 - 235 2.7e-26 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 157, 288
AccI GTMKAC 1 cut(s) 255
AccII CGCG 6 cut(s) 191, 210, 252, 328, 345, 598
AccIII TCCGGA 1 cut(s) 454
AcsI RAATTY 2 cut(s) 379, 671
AcyI GRCGYC 1 cut(s) 339
AfiI CCNNNNNNNGG 3 cut(s) 190, 321, 361
AflIII ACRYGT 1 cut(s) 326
AgsI TTSAA 2 cut(s) 506, 622
AjnI CCWGG 2 cut(s) 177, 824
AluBI AGCT 6 cut(s) 187, 695, 711, 739, 773, 853
AluI AGCT 6 cut(s) 187, 695, 711, 739, 773, 853
Alw21I GWGCWC 1 cut(s) 648
Alw26I GTCTC 4 cut(s) 4, 68, 220, 271
Ama87I CYCGRG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 5 cut(s) 123, 174, 180, 195, 405
ApaI GGGCCC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 379, 671
Asp700I GAANNNNTTC 2 cut(s) 617, 909
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 5 cut(s) 175, 181, 195, 196, 312
AsuC2I CCSGG 2 cut(s) 140, 322
AsuHPI GGTGA 2 cut(s) 151, 750
AsuNHI GCTAGC 1 cut(s) 853
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 199
BanII GRGCYC 2 cut(s) 199, 793
BbsI GAAGAC 4 cut(s) 18, 27, 33, 595
Bbv12I GWGCWC 1 cut(s) 648
BccI CCATC 2 cut(s) 22, 729
BciT130I CCWGG 2 cut(s) 179, 826
BcnI CCSGG 2 cut(s) 140, 322
BcoDI GTCTC 4 cut(s) 4, 68, 220, 271
BfaI CTAG 1 cut(s) 854
BisI GCNGC 3 cut(s) 123, 208, 471
BlpI GCTNAGC 1 cut(s) 712
BlsI GCNGC 3 cut(s) 124, 209, 472
Bme1390I CCNGG 4 cut(s) 140, 179, 322, 826
Bme18I GGWCC 1 cut(s) 312
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 5 cut(s) 175, 181, 195, 196, 312
BmiI GGNNCC 2 cut(s) 196, 197
BmrFI CCNGG 4 cut(s) 140, 179, 322, 826
BmsI GCATC 1 cut(s) 672
BmtI GCTAGC 1 cut(s) 857
BoxI GACNNNNGTC 1 cut(s) 329
BpiI GAAGAC 4 cut(s) 18, 27, 33, 595
Bpu1102I GCTNAGC 1 cut(s) 712
BpuMI CCSGG 2 cut(s) 140, 322
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 4 cut(s) 103, 138, 177, 824
BsaWI WCCGGW 1 cut(s) 454
BsaXI ACNNNNNCTCC 6 cut(s) 48, 78, 215, 245, 449, 479
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 321, 361
Bse1I ACTGG 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 2 cut(s) 179, 826
BseDI CCNNGG 4 cut(s) 103, 138, 177, 824
BseLI CCNNNNNNNGG 3 cut(s) 190, 321, 361
BseNI ACTGG 1 cut(s) 495
BseRI GAGGAG 2 cut(s) 362, 407
BseSI GKGCMC 1 cut(s) 199
BseYI CCCAGC 1 cut(s) 183
Bsh1236I CGCG 6 cut(s) 191, 210, 252, 328, 345, 598
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 5 cut(s) 125, 176, 182, 197, 407
BsiEI CGRYCG 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 648
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 4 cut(s) 140, 322, 355, 455
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 190, 321, 361
BsmAI GTCTC 4 cut(s) 4, 68, 220, 271
BsmBI CGTCTC 2 cut(s) 220, 271
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 5 cut(s) 125, 176, 182, 197, 407
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 242
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 199, 648, 793
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 2 cut(s) 303, 484
Bsp1720I GCTNAGC 1 cut(s) 712
BspANI GGCC 5 cut(s) 125, 176, 182, 197, 407
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 6 cut(s) 191, 210, 252, 328, 345, 598
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 2 cut(s) 196, 197
BspOI GCTAGC 1 cut(s) 857
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 2 cut(s) 157, 288
BsrI ACTGG 1 cut(s) 495
BssECI CCNNGG 4 cut(s) 103, 138, 177, 824
BssMI GATC 2 cut(s) 303, 484
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 2 cut(s) 179, 826
Bst4CI ACNGT 5 cut(s) 312, 631, 668, 818, 878
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 4 cut(s) 189, 697, 789, 855
BstDEI CTNAG 1 cut(s) 712
BstFNI CGCG 6 cut(s) 191, 210, 252, 328, 345, 598
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 2 cut(s) 306, 487
BstMAI GTCTC 4 cut(s) 4, 68, 220, 271
BstMBI GATC 2 cut(s) 303, 484
BstMCI CGRYCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 3 cut(s) 188, 351, 643
BstNI CCWGG 2 cut(s) 179, 826
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 4 cut(s) 138, 177, 320, 824
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 6 cut(s) 191, 210, 252, 328, 345, 598
BstV2I GAAGAC 4 cut(s) 18, 27, 33, 595
BsuRI GGCC 5 cut(s) 125, 176, 182, 197, 407
BtgZI GCGATG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 883
Cac8I GCNNGC 4 cut(s) 189, 697, 789, 855
CciI TCATGA 1 cut(s) 609
CfoI GCGC 1 cut(s) 252
Cfr13I GGNCC 5 cut(s) 175, 181, 195, 196, 312
CseI GACGC 5 cut(s) 77, 199, 241, 328, 334
CspCI CAANNNNNGTGG 2 cut(s) 533, 568
CviAII CATG 8 cut(s) 5, 60, 118, 529, 557, 584, 610, 893
DdeI CTNAG 1 cut(s) 712
DpnI GATC 2 cut(s) 305, 486
DpnII GATC 2 cut(s) 303, 484
EciI GGCGGA 4 cut(s) 194, 208, 457, 714
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 199, 793
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 195
EcoRII CCWGG 2 cut(s) 177, 824
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 199, 793
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 2 cut(s) 220, 271
FaeI CATG 8 cut(s) 8, 63, 121, 532, 560, 587, 613, 896
FaqI GGGAC 1 cut(s) 533
FatI CATG 8 cut(s) 4, 59, 117, 528, 556, 583, 609, 892
FauI CCCGC 3 cut(s) 162, 184, 293
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 123, 208, 471
FriOI GRGCYC 2 cut(s) 199, 793
Fsp4HI GCNGC 3 cut(s) 123, 208, 471
FspBI CTAG 1 cut(s) 854
GlaI GCGC 1 cut(s) 251
GluI GCNGC 3 cut(s) 123, 208, 471
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 5 cut(s) 125, 176, 182, 197, 407
HapII CCGG 4 cut(s) 140, 322, 355, 455
HgaI GACGC 5 cut(s) 77, 199, 241, 328, 334
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 8 cut(s) 8, 63, 121, 532, 560, 587, 613, 896
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HinfI GANTC 7 cut(s) 98, 230, 438, 464, 506, 836, 860
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 4 cut(s) 140, 322, 355, 455
HphI GGTGA 2 cut(s) 151, 750
Hpy166II GTNNAC 4 cut(s) 256, 481, 591, 627
Hpy188I TCNGA 2 cut(s) 308, 865
Hpy188III TCNNGA 6 cut(s) 67, 113, 376, 455, 503, 610
Hpy8I GTNNAC 4 cut(s) 256, 481, 591, 627
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 325, 397
HpyCH4III ACNGT 5 cut(s) 312, 631, 668, 818, 878
HpyCH4IV ACGT 2 cut(s) 32, 264
HpyCH4V TGCA 2 cut(s) 4, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 351, 643
HpyF3I CTNAG 1 cut(s) 712
HpySE526I ACGT 2 cut(s) 32, 264
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 8 cut(s) 8, 63, 121, 532, 560, 587, 613, 896
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 454
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 2 cut(s) 303, 484
LmnI GCTCC 3 cut(s) 293, 364, 692
LweI GCATC 1 cut(s) 672
MaeI CTAG 1 cut(s) 854
MaeII ACGT 2 cut(s) 32, 264
MaeIII GTNAC 2 cut(s) 89, 878
MalI GATC 2 cut(s) 305, 486
MbiI CCGCTC 2 cut(s) 157, 288
MboI GATC 2 cut(s) 303, 484
MboII GAAGA 9 cut(s) 9, 12, 18, 27, 33, 36, 39, 433, 595
MhlI GDGCHC 3 cut(s) 199, 648, 793
MluCI AATT 6 cut(s) 379, 573, 638, 671, 686, 922
MluI ACGCGT 1 cut(s) 326
MlyI GAGTC 3 cut(s) 224, 473, 854
MmeI TCCRAC 3 cut(s) 79, 258, 788
MnlI CCTC 6 cut(s) 340, 385, 414, 451, 510, 617
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 617, 909
MseI TTAA 3 cut(s) 411, 564, 590
MslI CAYNNNNRTG 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 4 cut(s) 140, 322, 355, 455
MspR9I CCNGG 4 cut(s) 140, 179, 322, 826
MvaI CCWGG 2 cut(s) 179, 826
MvnI CGCG 6 cut(s) 191, 210, 252, 328, 345, 598
MwoI GCNNNNNNNGC 3 cut(s) 188, 351, 643
NciI CCSGG 2 cut(s) 140, 322
NdeII GATC 2 cut(s) 303, 484
NheI GCTAGC 1 cut(s) 853
NlaIII CATG 8 cut(s) 8, 63, 121, 532, 560, 587, 613, 896
NlaIV GGNNCC 2 cut(s) 196, 197
NmuCI GTSAC 2 cut(s) 89, 878
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 609
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 219, 342
PdmI GAANNNNTTC 2 cut(s) 617, 909
PfeI GAWTC 4 cut(s) 98, 438, 506, 836
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 3 cut(s) 124, 209, 472
Ple19I CGATCG 1 cut(s) 487
PleI GAGTC 3 cut(s) 224, 472, 854
PpsI GAGTC 3 cut(s) 224, 472, 854
PshAI GACNNNNGTC 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 177, 824
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 2 cut(s) 177, 824
PspN4I GGNNCC 2 cut(s) 196, 197
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 5 cut(s) 175, 181, 195, 196, 312
PvuI CGATCG 1 cut(s) 487
RseI CAYNNNNRTG 1 cut(s) 527
SaqAI TTAA 3 cut(s) 411, 564, 590
SatI GCNGC 3 cut(s) 123, 208, 471
Sau3AI GATC 2 cut(s) 303, 484
Sau96I GGNCC 5 cut(s) 175, 181, 195, 196, 312
SchI GAGTC 3 cut(s) 224, 473, 854
ScrFI CCNGG 4 cut(s) 140, 179, 322, 826
SduI GDGCHC 3 cut(s) 199, 648, 793
SfaNI GCATC 1 cut(s) 672
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 527
Sse9I AATT 6 cut(s) 379, 573, 638, 671, 686, 922
SspMI CTAG 1 cut(s) 854
StyD4I CCNGG 4 cut(s) 138, 177, 320, 824
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 5 cut(s) 312, 631, 668, 818, 878
TaiI ACGT 2 cut(s) 35, 267
TaqI TCGA 2 cut(s) 642, 676
TasI AATT 6 cut(s) 379, 573, 638, 671, 686, 922
TauI GCSGC 3 cut(s) 125, 210, 473
TfiI GAWTC 4 cut(s) 98, 438, 506, 836
Tru1I TTAA 3 cut(s) 411, 564, 590
Tru9I TTAA 3 cut(s) 411, 564, 590
TscAI CASTG 1 cut(s) 883
TseFI GTSAC 2 cut(s) 89, 878
Tsp45I GTSAC 2 cut(s) 89, 878
TspDTI ATGAA 7 cut(s) 416, 545, 626, 820, 828, 909, 918
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 1 cut(s) 883
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 2 cut(s) 379, 671
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 2 cut(s) 617, 909
XspI CTAG 1 cut(s) 854
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.