MD04G1210300.v1.1

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010048
Physical Location & Seq
Reverse (-)
29489510 .. 29491787
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1210300.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCAGCAACAAAACTGCAAGAGCAGATTACAGCAAGTTCTACCGAAGCGGATAATCCTAATGCGGCACGGCGAGTCTCAAGGGAATCTCGACACCGCCGCCTACACCATCACCCCCGACAACAAAATCCCACTCACCCAAGAAGGCCTGGCCCAGGCCCAGCTCGCCGGCGCCGAGATCCACCGCGTCGTCTCCGACGCCGCCGGCGCTGACAGCCCCAACTGGAGCGTCTGCTTCTACGTCTCCCCTTACGAGCGGACGCGCTCCACGCTCAGCCAGATCGGACGGTCCTTCTCCAGGAAACGTGTGATCGGGGTCCGCGAGGAGTGCCGGATACGGGAGCAGGATTTCGGGAACTTCCAAGTGCAGGAGCGGATGAAGGCCATTAAGGAGACCAGGGAGCGGTTCGGCCGGTTCTTCTACCGGTTTCCGGAGGGGGAGTCCGCCGCCGACGTCTACGATCGCGTTTCGAGTTTTCTTGAATCCTTGTGGAGGGACATAGACATGAACAGGCTTCGCCATGACCCTTGCCACGACCTCAATCTCATAATCATATCACATGGGCTAACGTCACGCGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTCGAGCAATTCGAGCACCTAAACAACCTTGGGAATTGTGAATTTCGAGTGATGCAATTAGGAAAGGGTGGAGAGTACAGCTTAGCAATTCATCACACCGAGGAAGAACTGCAAGAATGGGGTCTATCACCCGAAATGATAGCCGACCAAAAGTGGCGAGCCCAAGCCAACAGGGGCGACTGGAATGAACGTTGCCCCTGGTACCTCGATGCTTTTTTCGACAAACTAGCTGAGCCGGAAGAAGATAGCGGAGACAGTGACGAGATCTGGCATGAAACTGATGAAAAAATTCCGCAAACGAATTGCAGTTCTTGA

Protein Analysis

307

Amino Acids

35.68

Weight (kDa)

5.48

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 18 - 227 5.7e-26 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 807
AccB1I GGYRCC 2 cut(s) 170, 807
AccBSI CCGCTC 3 cut(s) 256, 373, 403
AccI GTMKAC 1 cut(s) 456
AccII CGCG 5 cut(s) 186, 262, 321, 465, 576
AccIII TCCGGA 1 cut(s) 430
AclI AACGTT 1 cut(s) 796
AclWI GGATC 1 cut(s) 172
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 647, 894
AcyI GRCGYC 3 cut(s) 171, 198, 453
AfaI GTAC 2 cut(s) 683, 809
AfiI CCNNNNNNNGG 7 cut(s) 154, 297, 337, 367, 402, 430, 492
AflIII ACRYGT 2 cut(s) 304, 574
AgeI ACCGGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 482, 598
AjnI CCWGG 5 cut(s) 147, 153, 296, 395, 803
AluBI AGCT 3 cut(s) 163, 687, 836
AluI AGCT 3 cut(s) 163, 687, 836
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 5 cut(s) 81, 196, 247, 386, 852
AlwI GGATC 1 cut(s) 172
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 5 cut(s) 145, 150, 156, 381, 409
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 2 cut(s) 647, 894
AsiGI ACCGGT 1 cut(s) 423
Asp700I GAANNNNTTC 2 cut(s) 593, 894
Asp718I GGTACC 1 cut(s) 807
AspLEI GCGC 3 cut(s) 173, 209, 264
AspS9I GGNCC 4 cut(s) 151, 157, 288, 316
AsuHPI GGTGA 3 cut(s) 103, 127, 726
AvaII GGWCC 2 cut(s) 288, 316
BanI GGYRCC 2 cut(s) 170, 807
BanII GRGCYC 1 cut(s) 769
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 116
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 5 cut(s) 149, 155, 298, 397, 805
BciVI GTATCC 1 cut(s) 327
BcoDI GTCTC 5 cut(s) 81, 196, 247, 386, 852
BfaI CTAG 1 cut(s) 833
BfoI RGCGCY 2 cut(s) 174, 210
BfuI GTATCC 1 cut(s) 327
BglII AGATCT 1 cut(s) 870
BisI GCNGC 5 cut(s) 5, 65, 99, 201, 447
BlpI GCTNAGC 3 cut(s) 272, 688, 837
BlsI GCNGC 5 cut(s) 6, 66, 100, 202, 448
Bme1390I CCNGG 5 cut(s) 149, 155, 298, 397, 805
Bme18I GGWCC 2 cut(s) 288, 316
BmgT120I GGNCC 4 cut(s) 151, 157, 288, 316
BmiI GGNNCC 3 cut(s) 172, 317, 809
BmrFI CCNGG 5 cut(s) 149, 155, 298, 397, 805
BmsI GCATC 2 cut(s) 648, 805
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 2 cut(s) 244, 280
Bpu1102I GCTNAGC 3 cut(s) 272, 688, 837
BpuEI CTTGAG 1 cut(s) 63
BsaHI GRCGYC 3 cut(s) 171, 198, 453
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 5 cut(s) 153, 396, 634, 705, 803
BsaWI WCCGGW 2 cut(s) 423, 430
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 7 cut(s) 154, 297, 337, 367, 402, 430, 492
Bse118I RCCGGY 4 cut(s) 167, 203, 411, 423
Bse1I ACTGG 2 cut(s) 227, 791
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 5 cut(s) 149, 155, 298, 397, 805
BseDI CCNNGG 5 cut(s) 153, 396, 634, 705, 803
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 7 cut(s) 154, 297, 337, 367, 402, 430, 492
BseMII CTCAG 2 cut(s) 286, 828
BseNI ACTGG 2 cut(s) 227, 791
BseRI GAGGAG 1 cut(s) 338
BseX3I CGGCCG 1 cut(s) 409
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 386
Bsh1236I CGCG 5 cut(s) 186, 262, 321, 465, 576
Bsh1285I CGRYCG 3 cut(s) 412, 463, 609
BshFI GGCC 5 cut(s) 147, 152, 158, 383, 411
BshNI GGYRCC 2 cut(s) 170, 807
BshTI ACCGGT 1 cut(s) 423
BsiEI CGRYCG 3 cut(s) 412, 463, 609
BsiHKAI GWGCWC 1 cut(s) 624
BsiSI CCGG 7 cut(s) 168, 204, 331, 412, 424, 431, 842
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 7 cut(s) 154, 297, 337, 367, 402, 430, 492
BsmAI GTCTC 5 cut(s) 81, 196, 247, 386, 852
BsmBI CGTCTC 2 cut(s) 196, 247
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 5 cut(s) 147, 152, 158, 383, 411
Bso31I GGTCTC 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 624, 769
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 5 cut(s) 177, 279, 309, 460, 870
Bsp1720I GCTNAGC 3 cut(s) 272, 688, 837
BspANI GGCC 5 cut(s) 147, 152, 158, 383, 411
BspCNI CTCAG 2 cut(s) 285, 829
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 5 cut(s) 186, 262, 321, 465, 576
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 3 cut(s) 172, 317, 809
BspPI GGATC 1 cut(s) 172
BspT107I GGYRCC 2 cut(s) 170, 807
BspTNI GGTCTC 1 cut(s) 386
BsrBI CCGCTC 3 cut(s) 256, 373, 403
BsrFI RCCGGY 4 cut(s) 167, 203, 411, 423
BsrI ACTGG 2 cut(s) 227, 791
BssAI RCCGGY 4 cut(s) 167, 203, 411, 423
BssECI CCNNGG 5 cut(s) 153, 396, 634, 705, 803
BssMI GATC 5 cut(s) 177, 279, 309, 460, 870
BssNI GRCGYC 3 cut(s) 171, 198, 453
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 5 cut(s) 149, 155, 298, 397, 805
Bst4CI ACNGT 3 cut(s) 288, 607, 863
BstACI GRCGYC 3 cut(s) 171, 198, 453
BstC8I GCNNGC 4 cut(s) 165, 169, 205, 765
BstDEI CTNAG 3 cut(s) 272, 688, 837
BstENI CCTNNNNNAGG 3 cut(s) 152, 295, 490
BstF5I GGATG 1 cut(s) 381
BstFNI CGCG 5 cut(s) 186, 262, 321, 465, 576
BstH2I RGCGCY 2 cut(s) 174, 210
BstHHI GCGC 3 cut(s) 173, 209, 264
BstKTI GATC 5 cut(s) 180, 282, 312, 463, 873
BstMAI GTCTC 5 cut(s) 81, 196, 247, 386, 852
BstMBI GATC 5 cut(s) 177, 279, 309, 460, 870
BstMCI CGRYCG 3 cut(s) 412, 463, 609
BstMWI GCNNNNNNNGC 6 cut(s) 164, 206, 213, 268, 327, 619
BstNI CCWGG 5 cut(s) 149, 155, 298, 397, 805
BstSCI CCNGG 5 cut(s) 147, 153, 296, 395, 803
BstUI CGCG 5 cut(s) 186, 262, 321, 465, 576
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 2 cut(s) 177, 870
BstYI RGATCY 2 cut(s) 177, 870
BstZI CGGCCG 1 cut(s) 409
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 5 cut(s) 147, 152, 158, 383, 411
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 868
Cac8I GCNNGC 4 cut(s) 165, 169, 205, 765
CciI TCATGA 1 cut(s) 585
CfoI GCGC 3 cut(s) 173, 209, 264
Cfr10I RCCGGY 4 cut(s) 167, 203, 411, 423
Cfr13I GGNCC 4 cut(s) 151, 157, 288, 316
CseI GACGC 5 cut(s) 175, 206, 217, 268, 565
Csp6I GTAC 2 cut(s) 682, 808
CspAI ACCGGT 1 cut(s) 423
CviAII CATG 5 cut(s) 505, 521, 560, 586, 878
CviQI GTAC 2 cut(s) 682, 808
DdeI CTNAG 3 cut(s) 272, 688, 837
DinI GGCGCC 1 cut(s) 172
DpnI GATC 5 cut(s) 179, 281, 311, 462, 872
DpnII GATC 5 cut(s) 177, 279, 309, 460, 870
EaeI YGGCCR 1 cut(s) 409
EagI CGGCCG 1 cut(s) 409
EciI GGCGGA 1 cut(s) 433
EclXI CGGCCG 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 634
Eco147I AGGCCT 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 769
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 288, 316
Eco52I CGGCCG 1 cut(s) 409
EcoNI CCTNNNNNAGG 3 cut(s) 152, 295, 490
EcoRII CCWGG 5 cut(s) 147, 153, 296, 395, 803
EcoT14I CCWWGG 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 769
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 634
Esp3I CGTCTC 2 cut(s) 196, 247
FaeI CATG 5 cut(s) 508, 524, 563, 589, 881
FaiI YATR 8 cut(s) 500, 506, 522, 548, 554, 561, 587, 879
FaqI GGGAC 1 cut(s) 509
FatI CATG 5 cut(s) 504, 520, 559, 585, 877
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 5 cut(s) 5, 65, 99, 201, 447
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 5 cut(s) 5, 65, 99, 201, 447
FspBI CTAG 1 cut(s) 833
GlaI GCGC 3 cut(s) 172, 208, 263
GluI GCNGC 5 cut(s) 5, 65, 99, 201, 447
GsaI CCCAGC 1 cut(s) 163
GsuI CTGGAG 2 cut(s) 244, 280
HaeII RGCGCY 2 cut(s) 174, 210
HaeIII GGCC 5 cut(s) 147, 152, 158, 383, 411
HapII CCGG 7 cut(s) 168, 204, 331, 412, 424, 431, 842
HgaI GACGC 5 cut(s) 175, 206, 217, 268, 565
HhaI GCGC 3 cut(s) 173, 209, 264
Hin1I GRCGYC 3 cut(s) 171, 198, 453
Hin1II CATG 5 cut(s) 508, 524, 563, 589, 881
Hin6I GCGC 3 cut(s) 171, 207, 262
HinP1I GCGC 3 cut(s) 171, 207, 262
HinfI GANTC 4 cut(s) 74, 85, 440, 482
HpaII CCGG 7 cut(s) 168, 204, 331, 412, 424, 431, 842
HphI GGTGA 3 cut(s) 103, 127, 726
Hpy166II GTNNAC 2 cut(s) 457, 603
Hpy188I TCNGA 2 cut(s) 196, 284
Hpy188III TCNNGA 6 cut(s) 89, 352, 431, 479, 586, 918
Hpy8I GTNNAC 2 cut(s) 457, 603
Hpy99I CGWCG 3 cut(s) 191, 200, 455
HpyAV CCTTC 3 cut(s) 137, 301, 373
HpyCH4III ACNGT 3 cut(s) 288, 607, 863
HpyCH4IV ACGT 5 cut(s) 240, 304, 453, 569, 796
HpyCH4V TGCA 6 cut(s) 4, 18, 367, 661, 718, 912
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 206, 213, 268, 327, 619
HpyF3I CTNAG 3 cut(s) 272, 688, 837
HpySE526I ACGT 5 cut(s) 240, 304, 453, 569, 796
Hsp92I GRCGYC 3 cut(s) 171, 198, 453
Hsp92II CATG 5 cut(s) 508, 524, 563, 589, 881
HspAI GCGC 3 cut(s) 171, 207, 262
KasI GGCGCC 1 cut(s) 170
Kpn2I TCCGGA 1 cut(s) 430
KpnI GGTACC 1 cut(s) 811
KroI GCCGGC 2 cut(s) 167, 203
KroNI GCCGGC 2 cut(s) 169, 205
Kzo9I GATC 5 cut(s) 177, 279, 309, 460, 870
LmnI GCTCC 5 cut(s) 225, 269, 340, 370, 400
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 2 cut(s) 648, 805
MaeI CTAG 1 cut(s) 833
MaeII ACGT 5 cut(s) 240, 304, 453, 569, 796
MaeIII GTNAC 2 cut(s) 570, 863
MalI GATC 5 cut(s) 179, 281, 311, 462, 872
MbiI CCGCTC 3 cut(s) 256, 373, 403
MboI GATC 5 cut(s) 177, 279, 309, 460, 870
MboII GAAGA 5 cut(s) 409, 571, 722, 857, 860
MflI RGATCY 2 cut(s) 177, 870
MhlI GDGCHC 2 cut(s) 624, 769
MluCI AATT 7 cut(s) 614, 640, 647, 662, 693, 894, 907
MluI ACGCGT 1 cut(s) 574
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 2 cut(s) 83, 449
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 7 cut(s) 316, 427, 486, 548, 593, 700, 821
MreI CGCCGGCG 2 cut(s) 167, 203
MroI TCCGGA 1 cut(s) 430
MroNI GCCGGC 2 cut(s) 167, 203
MroXI GAANNNNTTC 2 cut(s) 593, 894
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 503
MspI CCGG 7 cut(s) 168, 204, 331, 412, 424, 431, 842
MspR9I CCNGG 5 cut(s) 149, 155, 298, 397, 805
MvaI CCWGG 5 cut(s) 149, 155, 298, 397, 805
MvnI CGCG 5 cut(s) 186, 262, 321, 465, 576
MwoI GCNNNNNNNGC 6 cut(s) 164, 206, 213, 268, 327, 619
NaeI GCCGGC 2 cut(s) 169, 205
NarI GGCGCC 1 cut(s) 171
NdeII GATC 5 cut(s) 177, 279, 309, 460, 870
NgoMIV GCCGGC 2 cut(s) 167, 203
NlaIII CATG 5 cut(s) 508, 524, 563, 589, 881
NlaIV GGNNCC 3 cut(s) 172, 317, 809
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 570, 863
PagI TCATGA 1 cut(s) 585
PceI AGGCCT 1 cut(s) 147
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 318, 615
PdiI GCCGGC 2 cut(s) 169, 205
PdmI GAANNNNTTC 2 cut(s) 593, 894
PfeI GAWTC 2 cut(s) 85, 482
PfoI TCCNGGA 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 5 cut(s) 6, 66, 100, 202, 448
Ple19I CGATCG 1 cut(s) 463
PleI GAGTC 2 cut(s) 82, 448
PluTI GGCGCC 1 cut(s) 174
PpsI GAGTC 2 cut(s) 82, 448
Psp1406I AACGTT 1 cut(s) 796
Psp6I CCWGG 5 cut(s) 147, 153, 296, 395, 803
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 5 cut(s) 147, 153, 296, 395, 803
PspN4I GGNNCC 3 cut(s) 172, 317, 809
PspPI GGNCC 4 cut(s) 151, 157, 288, 316
PsuI RGATCY 2 cut(s) 177, 870
PvuI CGATCG 1 cut(s) 463
RsaI GTAC 2 cut(s) 683, 809
RsaNI GTAC 2 cut(s) 682, 808
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 5 cut(s) 5, 65, 99, 201, 447
Sau3AI GATC 5 cut(s) 177, 279, 309, 460, 870
Sau96I GGNCC 4 cut(s) 151, 157, 288, 316
SchI GAGTC 2 cut(s) 83, 449
ScrFI CCNGG 5 cut(s) 149, 155, 298, 397, 805
SduI GDGCHC 2 cut(s) 624, 769
SfaNI GCATC 2 cut(s) 648, 805
SfoI GGCGCC 1 cut(s) 172
SgrAI CRCCGGYG 2 cut(s) 167, 203
SinI GGWCC 2 cut(s) 288, 316
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 7 cut(s) 614, 640, 647, 662, 693, 894, 907
SseBI AGGCCT 1 cut(s) 147
SspDI GGCGCC 1 cut(s) 170
SspMI CTAG 1 cut(s) 833
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 5 cut(s) 147, 153, 296, 395, 803
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 3 cut(s) 288, 607, 863
TaiI ACGT 5 cut(s) 243, 307, 456, 572, 799
TaqI TCGA 7 cut(s) 90, 470, 609, 618, 652, 813, 825
TasI AATT 7 cut(s) 614, 640, 647, 662, 693, 894, 907
TatI WGTACW 1 cut(s) 681
TauI GCSGC 4 cut(s) 67, 101, 203, 449
TfiI GAWTC 2 cut(s) 85, 482
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 868
TseFI GTSAC 2 cut(s) 570, 863
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 2 cut(s) 570, 863
TspDTI ATGAA 7 cut(s) 392, 521, 602, 686, 807, 894, 903
TspRI CASTG 1 cut(s) 868
VpaK11BI GGWCC 2 cut(s) 288, 316
XagI CCTNNNNNAGG 3 cut(s) 152, 295, 490
XapI RAATTY 2 cut(s) 647, 894
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 2 cut(s) 593, 894
XspI CTAG 1 cut(s) 833
ZraI GACGTC 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.