MD12G1224600.v1.1

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010048
Physical Location & Seq
Reverse (-)
30072443 .. 30074258
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1224600.v1.1.491

Sequence Viewer

Length: 912 bp
ATGCAGCAACAAAATTGCAAGAGGAGATTACAGCCAGTTCTACCGAAGCGGATAATCCTAATGCGGCATGGCGAGTCCGAAGGGAATCTCGATCACGCCGCCTACACCACCACCCCCGACAACAAAATCCCACTCACCCAAGAGGGCCTGGCACAGGCCCGGCTCGCCGGGGCCGAGCTCCACCGCGTCGTCTCCGACACCACCGGCGCCGACAGCCCCAACTGGCGCATCTACTTCTACGTCTCCCCTTACGAGCGGACGCGGTCCACGCTCTGCCAGATCGGACGGTCATTCTCCAGGACGCGGATCATCGGGGTCCGCGAGGAGTGCCGGATACGGGAGCAGGATTTCGGGAACTTCCAAGAGCAGGATCGGATGAAGGCCACTAAGGAGACCAGGGAGCAGTTCGGCCGGTTCTTCTACCGGTTTCCGGAGGGGGAGTCCGCCGCCGACGTCTACGATCGCGTTTCGAGTTTTCTTGAATCCTTATGGAGGGACATAGACATGAACAGGCTTCGCCACGACCCTTGCCACGACCTCAATCTTATAATAATATCGCACGGGTTAGCATCACGCGTCTTCCTCATGAAGTGGTTCAAGTGGACGGTCGAGCAATTTGAGCTCCTAATCAACCTTGGAAATTGCGAATTTCGAGTGATGCAATTAGGAAAGGGTGGAGAGTACAGCTTAGCAATTCATCACACTGAGGAAGAACTACAAGAATGGGGTCTCTCACCCGAAATGATAGCGGACCAAAAGTGGCGAGCCCGCGCCAACAGGGTTGATTGGACTGAACGTTGCCCCTGGTACCTTGATTCATTTTTCGACCAACTAGCTGAGTCGGAAGAAGACAACGGAGACAGTGACGACATTTGGCATGAAACTGATGAAAAAATTCCGCAAACGAATTGA

Protein Analysis

304

Amino Acids

35.7

Weight (kDa)

5.39

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 18 - 227 4.4e-26 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AatII GACGTC 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 807
AccB1I GGYRCC 2 cut(s) 206, 807
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 456
AccII CGCG 7 cut(s) 186, 262, 304, 321, 465, 576, 771
AccIII TCCGGA 1 cut(s) 430
AclI AACGTT 1 cut(s) 796
AclWI GGATC 2 cut(s) 314, 378
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 647, 894
AcyI GRCGYC 2 cut(s) 207, 453
AfaI GTAC 2 cut(s) 683, 809
AfiI CCNNNNNNNGG 6 cut(s) 154, 303, 337, 367, 430, 492
AflIII ACRYGT 1 cut(s) 574
AgeI ACCGGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 482, 598
AjnI CCWGG 4 cut(s) 147, 296, 395, 803
AluBI AGCT 4 cut(s) 178, 622, 687, 836
AluI AGCT 4 cut(s) 178, 622, 687, 836
Alw21I GWGCWC 2 cut(s) 180, 624
Alw26I GTCTC 5 cut(s) 196, 247, 386, 734, 852
AlwI GGATC 2 cut(s) 314, 378
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 5 cut(s) 145, 156, 171, 381, 409
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 2 cut(s) 647, 894
AsiGI ACCGGT 1 cut(s) 423
Asp700I GAANNNNTTC 2 cut(s) 593, 894
Asp718I GGTACC 1 cut(s) 807
AspLEI GCGC 3 cut(s) 209, 228, 773
AspS9I GGNCC 6 cut(s) 145, 157, 171, 264, 316, 751
AsuC2I CCSGG 2 cut(s) 160, 169
AsuHPI GGTGA 2 cut(s) 127, 726
AvaII GGWCC 3 cut(s) 264, 316, 751
BanI GGYRCC 2 cut(s) 206, 807
BanII GRGCYC 3 cut(s) 180, 624, 769
BbsI GAAGAC 2 cut(s) 571, 855
Bbv12I GWGCWC 2 cut(s) 180, 624
BbvI GCAGC 1 cut(s) 16
BciT130I CCWGG 4 cut(s) 149, 298, 397, 805
BciVI GTATCC 1 cut(s) 327
BcnI CCSGG 2 cut(s) 160, 169
BcoDI GTCTC 5 cut(s) 196, 247, 386, 734, 852
BfaI CTAG 1 cut(s) 833
BfoI RGCGCY 1 cut(s) 210
BfuI GTATCC 1 cut(s) 327
BisI GCNGC 4 cut(s) 5, 65, 99, 447
BlpI GCTNAGC 1 cut(s) 688
BlsI GCNGC 4 cut(s) 6, 66, 100, 448
Bme1390I CCNGG 6 cut(s) 149, 160, 169, 298, 397, 805
Bme18I GGWCC 3 cut(s) 264, 316, 751
BmgT120I GGNCC 6 cut(s) 145, 157, 171, 264, 316, 751
BmiI GGNNCC 4 cut(s) 172, 208, 317, 809
BmrFI CCNGG 6 cut(s) 149, 160, 169, 298, 397, 805
BmsI GCATC 3 cut(s) 237, 578, 648
BpiI GAAGAC 2 cut(s) 571, 855
BpmI CTGGAG 1 cut(s) 280
Bpu1102I GCTNAGC 1 cut(s) 688
BpuMI CCSGG 2 cut(s) 160, 169
BsaHI GRCGYC 2 cut(s) 207, 453
BsaI GGTCTC 2 cut(s) 386, 734
BsaJI CCNNGG 4 cut(s) 168, 396, 634, 803
BsaWI WCCGGW 2 cut(s) 423, 430
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 6 cut(s) 154, 303, 337, 367, 430, 492
Bse118I RCCGGY 3 cut(s) 203, 411, 423
Bse1I ACTGG 2 cut(s) 35, 227
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 4 cut(s) 149, 298, 397, 805
BseDI CCNNGG 4 cut(s) 168, 396, 634, 803
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 6 cut(s) 154, 303, 337, 367, 430, 492
BseMII CTCAG 2 cut(s) 696, 828
BseNI ACTGG 2 cut(s) 35, 227
BseRI GAGGAG 2 cut(s) 37, 338
BseX3I CGGCCG 1 cut(s) 409
BseXI GCAGC 1 cut(s) 16
Bsh1236I CGCG 7 cut(s) 186, 262, 304, 321, 465, 576, 771
Bsh1285I CGRYCG 3 cut(s) 412, 463, 609
BshFI GGCC 5 cut(s) 147, 158, 173, 383, 411
BshNI GGYRCC 2 cut(s) 206, 807
BshTI ACCGGT 1 cut(s) 423
BsiEI CGRYCG 3 cut(s) 412, 463, 609
BsiHKAI GWGCWC 2 cut(s) 180, 624
BsiSI CCGG 7 cut(s) 160, 168, 204, 331, 412, 424, 431
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 6 cut(s) 154, 303, 337, 367, 430, 492
BsmAI GTCTC 5 cut(s) 196, 247, 386, 734, 852
BsmBI CGTCTC 2 cut(s) 196, 247
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 5 cut(s) 147, 158, 173, 383, 411
Bso31I GGTCTC 2 cut(s) 386, 734
Bsp1286I GDGCHC 3 cut(s) 180, 624, 769
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 5 cut(s) 91, 279, 306, 370, 460
Bsp1720I GCTNAGC 1 cut(s) 688
BspANI GGCC 5 cut(s) 147, 158, 173, 383, 411
BspCNI CTCAG 2 cut(s) 697, 829
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 7 cut(s) 186, 262, 304, 321, 465, 576, 771
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 4 cut(s) 172, 208, 317, 809
BspPI GGATC 2 cut(s) 314, 378
BspT107I GGYRCC 2 cut(s) 206, 807
BspTNI GGTCTC 2 cut(s) 386, 734
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 3 cut(s) 203, 411, 423
BsrI ACTGG 2 cut(s) 35, 227
BssAI RCCGGY 3 cut(s) 203, 411, 423
BssECI CCNNGG 4 cut(s) 168, 396, 634, 803
BssMI GATC 5 cut(s) 91, 279, 306, 370, 460
BssNI GRCGYC 2 cut(s) 207, 453
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 4 cut(s) 149, 298, 397, 805
Bst4CI ACNGT 3 cut(s) 288, 607, 863
BstACI GRCGYC 2 cut(s) 207, 453
BstC8I GCNNGC 3 cut(s) 165, 765, 769
BstDEI CTNAG 4 cut(s) 387, 688, 705, 837
BstENI CCTNNNNNAGG 2 cut(s) 152, 490
BstF5I GGATG 1 cut(s) 381
BstFNI CGCG 7 cut(s) 186, 262, 304, 321, 465, 576, 771
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 3 cut(s) 209, 228, 773
BstKTI GATC 5 cut(s) 94, 282, 309, 373, 463
BstMAI GTCTC 5 cut(s) 196, 247, 386, 734, 852
BstMBI GATC 5 cut(s) 91, 279, 306, 370, 460
BstMCI CGRYCG 3 cut(s) 412, 463, 609
BstMWI GCNNNNNNNGC 5 cut(s) 164, 213, 268, 327, 619
BstNI CCWGG 4 cut(s) 149, 298, 397, 805
BstSCI CCNGG 6 cut(s) 147, 158, 167, 296, 395, 803
BstUI CGCG 7 cut(s) 186, 262, 304, 321, 465, 576, 771
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 2 cut(s) 571, 855
BstZI CGGCCG 1 cut(s) 409
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 5 cut(s) 147, 158, 173, 383, 411
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 702, 868
Cac8I GCNNGC 3 cut(s) 165, 765, 769
CciI TCATGA 1 cut(s) 585
CfoI GCGC 3 cut(s) 209, 228, 773
Cfr10I RCCGGY 3 cut(s) 203, 411, 423
Cfr13I GGNCC 6 cut(s) 145, 157, 171, 264, 316, 751
CseI GACGC 4 cut(s) 175, 268, 310, 565
Csp6I GTAC 2 cut(s) 682, 808
CspAI ACCGGT 1 cut(s) 423
CspCI CAANNNNNGTGG 2 cut(s) 509, 544
CviAII CATG 4 cut(s) 68, 505, 586, 878
CviQI GTAC 2 cut(s) 682, 808
DdeI CTNAG 4 cut(s) 387, 688, 705, 837
DinI GGCGCC 1 cut(s) 208
DpnI GATC 5 cut(s) 93, 281, 308, 372, 462
DpnII GATC 5 cut(s) 91, 279, 306, 370, 460
EaeI YGGCCR 1 cut(s) 409
EagI CGGCCG 1 cut(s) 409
EciI GGCGGA 1 cut(s) 433
Ecl136II GAGCTC 2 cut(s) 178, 622
EclXI CGGCCG 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 634
Eco24I GRGCYC 3 cut(s) 180, 624, 769
Eco31I GGTCTC 2 cut(s) 386, 734
Eco47I GGWCC 3 cut(s) 264, 316, 751
Eco52I CGGCCG 1 cut(s) 409
Eco53kI GAGCTC 2 cut(s) 178, 622
EcoICRI GAGCTC 2 cut(s) 178, 622
EcoNI CCTNNNNNAGG 2 cut(s) 152, 490
EcoO109I RGGNCCY 1 cut(s) 145
EcoRII CCWGG 4 cut(s) 147, 296, 395, 803
EcoT14I CCWWGG 1 cut(s) 634
EcoT38I GRGCYC 3 cut(s) 180, 624, 769
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 634
Esp3I CGTCTC 2 cut(s) 196, 247
FaeI CATG 4 cut(s) 71, 508, 589, 881
FaiI YATR 7 cut(s) 69, 490, 500, 506, 548, 587, 879
FaqI GGGAC 1 cut(s) 509
FatI CATG 4 cut(s) 67, 504, 585, 877
FauI CCCGC 1 cut(s) 776
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 4 cut(s) 5, 65, 99, 447
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 3 cut(s) 180, 624, 769
Fsp4HI GCNGC 4 cut(s) 5, 65, 99, 447
FspBI CTAG 1 cut(s) 833
GlaI GCGC 3 cut(s) 208, 227, 772
GluI GCNGC 4 cut(s) 5, 65, 99, 447
GsuI CTGGAG 1 cut(s) 280
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 5 cut(s) 147, 158, 173, 383, 411
HapII CCGG 7 cut(s) 160, 168, 204, 331, 412, 424, 431
HgaI GACGC 4 cut(s) 175, 268, 310, 565
HhaI GCGC 3 cut(s) 209, 228, 773
Hin1I GRCGYC 2 cut(s) 207, 453
Hin1II CATG 4 cut(s) 71, 508, 589, 881
Hin6I GCGC 3 cut(s) 207, 226, 771
HinP1I GCGC 3 cut(s) 207, 226, 771
HinfI GANTC 6 cut(s) 74, 85, 440, 482, 815, 839
HpaII CCGG 7 cut(s) 160, 168, 204, 331, 412, 424, 431
HphI GGTGA 2 cut(s) 127, 726
Hpy166II GTNNAC 3 cut(s) 267, 457, 603
Hpy188I TCNGA 5 cut(s) 79, 196, 284, 375, 844
Hpy188III TCNNGA 5 cut(s) 89, 352, 431, 479, 586
Hpy8I GTNNAC 3 cut(s) 267, 457, 603
Hpy99I CGWCG 2 cut(s) 191, 455
HpyAV CCTTC 2 cut(s) 74, 373
HpyCH4III ACNGT 3 cut(s) 288, 607, 863
HpyCH4IV ACGT 3 cut(s) 240, 453, 796
HpyCH4V TGCA 3 cut(s) 4, 18, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 213, 268, 327, 619
HpyF3I CTNAG 4 cut(s) 387, 688, 705, 837
HpySE526I ACGT 3 cut(s) 240, 453, 796
Hsp92I GRCGYC 2 cut(s) 207, 453
Hsp92II CATG 4 cut(s) 71, 508, 589, 881
HspAI GCGC 3 cut(s) 207, 226, 771
KasI GGCGCC 1 cut(s) 206
Kpn2I TCCGGA 1 cut(s) 430
KpnI GGTACC 1 cut(s) 811
Kzo9I GATC 5 cut(s) 91, 279, 306, 370, 460
LmnI GCTCC 4 cut(s) 183, 340, 400, 627
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 237, 578, 648
MaeI CTAG 1 cut(s) 833
MaeII ACGT 3 cut(s) 240, 453, 796
MaeIII GTNAC 1 cut(s) 863
MalI GATC 5 cut(s) 93, 281, 308, 372, 462
MbiI CCGCTC 1 cut(s) 256
MboI GATC 5 cut(s) 91, 279, 306, 370, 460
MboII GAAGA 5 cut(s) 409, 571, 722, 857, 860
MhlI GDGCHC 3 cut(s) 180, 624, 769
MluCI AATT 8 cut(s) 13, 614, 640, 647, 662, 693, 894, 907
MluI ACGCGT 1 cut(s) 574
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 3 cut(s) 83, 449, 848
MmeI TCCRAC 2 cut(s) 219, 822
MnlI CCTC 8 cut(s) 15, 136, 316, 427, 486, 548, 593, 700
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 2 cut(s) 593, 894
MslI CAYNNNNRTG 1 cut(s) 503
MspI CCGG 7 cut(s) 160, 168, 204, 331, 412, 424, 431
MspR9I CCNGG 6 cut(s) 149, 160, 169, 298, 397, 805
MvaI CCWGG 4 cut(s) 149, 298, 397, 805
MvnI CGCG 7 cut(s) 186, 262, 304, 321, 465, 576, 771
MwoI GCNNNNNNNGC 5 cut(s) 164, 213, 268, 327, 619
NarI GGCGCC 1 cut(s) 207
NciI CCSGG 2 cut(s) 160, 169
NdeII GATC 5 cut(s) 91, 279, 306, 370, 460
NlaIII CATG 4 cut(s) 71, 508, 589, 881
NlaIV GGNNCC 4 cut(s) 172, 208, 317, 809
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 863
PagI TCATGA 1 cut(s) 585
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 318
PdmI GAANNNNTTC 2 cut(s) 593, 894
PfeI GAWTC 3 cut(s) 85, 482, 815
PflFI GACNNNGTC 1 cut(s) 262
PfoI TCCNGGA 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 4 cut(s) 6, 66, 100, 448
Ple19I CGATCG 1 cut(s) 463
PleI GAGTC 3 cut(s) 82, 448, 847
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 3 cut(s) 82, 448, 847
PsiI TTATAA 1 cut(s) 548
Psp124BI GAGCTC 2 cut(s) 180, 624
Psp1406I AACGTT 1 cut(s) 796
Psp6I CCWGG 4 cut(s) 147, 296, 395, 803
PspGI CCWGG 4 cut(s) 147, 296, 395, 803
PspN4I GGNNCC 4 cut(s) 172, 208, 317, 809
PspPI GGNCC 6 cut(s) 145, 157, 171, 264, 316, 751
PsyI GACNNNGTC 1 cut(s) 262
PvuI CGATCG 1 cut(s) 463
RsaI GTAC 2 cut(s) 683, 809
RsaNI GTAC 2 cut(s) 682, 808
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 2 cut(s) 180, 624
SatI GCNGC 4 cut(s) 5, 65, 99, 447
Sau3AI GATC 5 cut(s) 91, 279, 306, 370, 460
Sau96I GGNCC 6 cut(s) 145, 157, 171, 264, 316, 751
SchI GAGTC 3 cut(s) 83, 449, 848
ScrFI CCNGG 6 cut(s) 149, 160, 169, 298, 397, 805
SduI GDGCHC 3 cut(s) 180, 624, 769
SfaNI GCATC 3 cut(s) 237, 578, 648
SfoI GGCGCC 1 cut(s) 208
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 3 cut(s) 264, 316, 751
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 8 cut(s) 13, 614, 640, 647, 662, 693, 894, 907
SspDI GGCGCC 1 cut(s) 206
SspMI CTAG 1 cut(s) 833
SstI GAGCTC 2 cut(s) 180, 624
StyD4I CCNGG 6 cut(s) 147, 158, 167, 296, 395, 803
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 3 cut(s) 288, 607, 863
TaiI ACGT 3 cut(s) 243, 456, 799
TaqI TCGA 5 cut(s) 90, 470, 609, 652, 825
TasI AATT 8 cut(s) 13, 614, 640, 647, 662, 693, 894, 907
TatI WGTACW 1 cut(s) 681
TauI GCSGC 3 cut(s) 67, 101, 449
TfiI GAWTC 3 cut(s) 85, 482, 815
TscAI CASTG 2 cut(s) 709, 868
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 7 cut(s) 392, 521, 602, 686, 807, 894, 903
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 2 cut(s) 709, 868
Tth111I GACNNNGTC 1 cut(s) 262
VpaK11BI GGWCC 3 cut(s) 264, 316, 751
XagI CCTNNNNNAGG 2 cut(s) 152, 490
XapI RAATTY 2 cut(s) 647, 894
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 2 cut(s) 593, 894
XspI CTAG 1 cut(s) 833
ZraI GACGTC 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.