AT1G10310

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010491
Physical Location & Seq
Reverse (-)
3381447 .. 3384003
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10310.1

Sequence Viewer

Length: 729 bp
ATGACGATGGCGACGCCTTTCAGTGGGGCTGCGAACTCTATTGTGGCGGCGAGGACCGTTCTGATAACGGGAGTGAGCAAAGGTCTGGGAAGAGCCCTAGCTCTCGAGCTGGCCAAGAGGGGTCACACTGTGATAGGATGCGCTCGTAGTCAAGAGAAGCTCACCGCTCTTCAATCGGAGCTTTCCTCTTCGACCAATCATCTCCTCCTCACCGCTGATGTGAAATCAAACAGCAGTGTCGAAGAAATGGCACACACAATTGTGGAGAAGAAGGGTGTCCCAGATATCATAGTTAACAATGCTGGTACCATCAATAAGAACAGCAAGATTTGGGAAGTCTCTGCTGAAGATTTTGACAATGTCATGGATACAAACGTTAAAGGAGTTGCAAATGTGCTGCGCCATTTTATCCCTTTGATGCTCCCACGCAAGCAAGGAATTATTGTAAATATGTCTTCTGGATGGGGAAGATCTGGTGCTGCCCTGGTTGCACCTTACTGTGCATCAAAATGGGCGATTGAGGGTTTAAGCCGAGCAGTGGCAAAGGAAGTAGTTGAAGGAATGGCAGTAGTTGCATTAAATCCAGGCGTGATCAACACAGAATTACTCACTTCCTGCTTCGGGAACTCAGCTTCCTTGTATCAAGCTCCTGATGCTTGGGCTGTGAAGGCTGCGACGATGATTCTGAATCTTACAGCGGGTGACAATGGCGGATCTCTCACAGTCTAA

Protein Analysis

242

Amino Acids

25.32

Weight (kDa)

8.44

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 19 - 205 1.7e-48 short chain dehydrogenase
KR PF08659 19 - 189 2.7e-12 KR domain
Epimerase PF01370 20 - 193 2.5e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 24 - 210 9.4e-36 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 167
AciI CCGC 5 cut(s) 47, 165, 213, 698, 711
AclI AACGTT 1 cut(s) 375
AclWI GGATC 1 cut(s) 721
AcoI YGGCCR 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 366
AcyI GRCGYC 1 cut(s) 14
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 3 cut(s) 23, 538, 621
AgsI TTSAA 2 cut(s) 173, 557
AjnI CCWGG 2 cut(s) 483, 583
AleI CACNNNNGTG 1 cut(s) 260
AloI GAACNNNNNNTCC 2 cut(s) 617, 649
AluBI AGCT 6 cut(s) 101, 109, 160, 181, 632, 647
AluI AGCT 6 cut(s) 101, 109, 160, 181, 632, 647
Alw26I GTCTC 1 cut(s) 343
AlwI GGATC 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 4 cut(s) 29, 397, 479, 671
Asp718I GGTACC 1 cut(s) 305
AspLEI GCGC 2 cut(s) 143, 402
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 3 cut(s) 154, 202, 713
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 54
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BalI TGGCCA 1 cut(s) 113
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 4 cut(s) 16, 384, 466, 658
BccI CCATC 2 cut(s) 317, 456
BciT130I CCWGG 2 cut(s) 485, 585
BciVI GTATCC 1 cut(s) 361
BclI TGATCA 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 343
BfaI CTAG 1 cut(s) 98
BfuI GTATCC 1 cut(s) 361
BglII AGATCT 1 cut(s) 470
BisI GCNGC 5 cut(s) 30, 48, 398, 480, 672
BlsI GCNGC 5 cut(s) 31, 49, 399, 481, 673
Bme1390I CCNGG 2 cut(s) 485, 585
Bme18I GGWCC 1 cut(s) 54
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 2 cut(s) 485, 585
BmsI GCATC 4 cut(s) 128, 408, 512, 643
BpiI GAAGAC 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 483
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 538, 621
BseBI CCWGG 2 cut(s) 485, 585
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 2 cut(s) 143, 467
BseLI CCNNNNNNNGG 3 cut(s) 23, 538, 621
BseMII CTCAG 1 cut(s) 642
BseRI GAGGAG 2 cut(s) 194, 197
BseXI GCAGC 4 cut(s) 16, 384, 466, 658
BshFI GGCC 1 cut(s) 113
BshNI GGYRCC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 3 cut(s) 23, 538, 621
BsmAI GTCTC 1 cut(s) 343
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 3 cut(s) 470, 591, 713
BspACI CCGC 5 cut(s) 47, 165, 213, 698, 711
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 1 cut(s) 641
BspLI GGNNCC 1 cut(s) 307
BspPI GGATC 1 cut(s) 721
BspQI GCTCTTC 2 cut(s) 85, 174
BspT107I GGYRCC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 3 cut(s) 470, 591, 713
BssNI GRCGYC 1 cut(s) 14
Bst2UI CCWGG 2 cut(s) 485, 585
Bst4CI ACNGT 4 cut(s) 58, 130, 500, 724
Bst6I CTCTTC 3 cut(s) 85, 174, 193
BstACI GRCGYC 1 cut(s) 14
BstAPI GCANNNNNTGC 1 cut(s) 572
BstC8I GCNNGC 2 cut(s) 111, 431
BstDEI CTNAG 1 cut(s) 628
BstF5I GGATG 2 cut(s) 143, 467
BstHHI GCGC 2 cut(s) 143, 402
BstKTI GATC 3 cut(s) 473, 594, 716
BstMAI GTCTC 1 cut(s) 343
BstMBI GATC 3 cut(s) 470, 591, 713
BstMWI GCNNNNNNNGC 4 cut(s) 488, 572, 653, 668
BstNI CCWGG 2 cut(s) 485, 585
BstSCI CCNGG 2 cut(s) 483, 583
BstV1I GCAGC 4 cut(s) 16, 384, 466, 658
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 2 cut(s) 470, 713
BstYI RGATCY 2 cut(s) 470, 713
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 1 cut(s) 113
BtsCI GGATG 2 cut(s) 143, 467
BtsI GCAGTG 2 cut(s) 241, 543
BtsIMutI CAGTG 4 cut(s) 28, 126, 241, 543
Cac8I GCNNGC 2 cut(s) 111, 431
CfoI GCGC 2 cut(s) 143, 402
Cfr13I GGNCC 1 cut(s) 54
CseI GACGC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 306
CviAII CATG 1 cut(s) 364
CviQI GTAC 1 cut(s) 306
DdeI CTNAG 1 cut(s) 628
DpnI GATC 3 cut(s) 472, 593, 715
DpnII GATC 3 cut(s) 470, 591, 713
DraIII CACNNNGTG 1 cut(s) 130
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 3 cut(s) 85, 174, 193
EarI CTCTTC 3 cut(s) 85, 174, 193
EciI GGCGGA 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 97
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 483, 583
EcoRV GATATC 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 97
FaeI CATG 1 cut(s) 367
FaiI YATR 3 cut(s) 290, 365, 452
FaqI GGGAC 1 cut(s) 263
FatI CATG 1 cut(s) 363
FauI CCCGC 1 cut(s) 691
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 5 cut(s) 30, 48, 398, 480, 672
FokI GGATG 2 cut(s) 150, 474
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 5 cut(s) 30, 48, 398, 480, 672
FspBI CTAG 1 cut(s) 98
GlaI GCGC 2 cut(s) 142, 401
GluI GCNGC 5 cut(s) 30, 48, 398, 480, 672
HaeIII GGCC 1 cut(s) 113
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 143, 402
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 2 cut(s) 141, 400
HinP1I GCGC 2 cut(s) 141, 400
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 2 cut(s) 682, 688
HpaI GTTAAC 1 cut(s) 295
HphI GGTGA 3 cut(s) 154, 202, 713
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 63, 178, 687
Hpy188III TCNNGA 5 cut(s) 104, 152, 459, 622, 650
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 2 cut(s) 16, 679
HpyAV CCTTC 3 cut(s) 265, 551, 661
HpyCH4III ACNGT 4 cut(s) 58, 130, 500, 724
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 4 cut(s) 389, 491, 503, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 488, 572, 653, 668
HpyF3I CTNAG 1 cut(s) 628
HpySE526I ACGT 1 cut(s) 375
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 2 cut(s) 141, 400
KpnI GGTACC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 591
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 3 cut(s) 470, 591, 713
LguI GCTCTTC 2 cut(s) 85, 174
LmnI GCTCC 3 cut(s) 178, 426, 652
Lsp1109I GCAGC 4 cut(s) 16, 384, 466, 658
LweI GCATC 4 cut(s) 128, 408, 512, 643
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 122, 701
MalI GATC 3 cut(s) 472, 593, 715
MbiI CCGCTC 1 cut(s) 167
MboI GATC 3 cut(s) 470, 591, 713
MboII GAAGA 8 cut(s) 102, 161, 180, 254, 280, 359, 447, 480
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 2 cut(s) 470, 713
MhlI GDGCHC 1 cut(s) 97
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 3 cut(s) 258, 438, 602
MluNI TGGCCA 1 cut(s) 113
MnlI CCTC 6 cut(s) 45, 111, 196, 215, 218, 514
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
MseI TTAA 4 cut(s) 294, 378, 527, 578
MslI CAYNNNNRTG 2 cut(s) 260, 508
Msp20I TGGCCA 1 cut(s) 113
MspA1I CMGCKG 2 cut(s) 215, 698
MspR9I CCNGG 2 cut(s) 485, 585
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 2 cut(s) 485, 585
MwoI GCNNNNNNNGC 4 cut(s) 488, 572, 653, 668
NdeII GATC 3 cut(s) 470, 591, 713
NlaIII CATG 1 cut(s) 367
NlaIV GGNNCC 1 cut(s) 307
NmeAIII GCCGAG 1 cut(s) 557
NmuCI GTSAC 2 cut(s) 122, 701
OliI CACNNNNGTG 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 104
PciSI GCTCTTC 2 cut(s) 85, 174
PfeI GAWTC 2 cut(s) 682, 688
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 5 cut(s) 31, 49, 399, 481, 673
Psp1406I AACGTT 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 483, 583
PspGI CCWGG 2 cut(s) 483, 583
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 54
PsuI RGATCY 2 cut(s) 470, 713
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
RseI CAYNNNNRTG 2 cut(s) 260, 508
SapI GCTCTTC 2 cut(s) 85, 174
SaqAI TTAA 4 cut(s) 294, 378, 527, 578
SatI GCNGC 5 cut(s) 30, 48, 398, 480, 672
Sau3AI GATC 3 cut(s) 470, 591, 713
Sau96I GGNCC 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 485, 585
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 85, 103, 111, 162, 183, 378, 496, 634, 649
SfaNI GCATC 4 cut(s) 128, 408, 512, 643
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 54
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 260, 508
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 3 cut(s) 258, 438, 602
SsiI CCGC 5 cut(s) 47, 165, 213, 698, 711
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 483, 583
TaaI ACNGT 4 cut(s) 58, 130, 500, 724
TaiI ACGT 1 cut(s) 378
TaqI TCGA 3 cut(s) 105, 191, 240
TasI AATT 3 cut(s) 258, 438, 602
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 682, 688
Tru1I TTAA 4 cut(s) 294, 378, 527, 578
Tru9I TTAA 4 cut(s) 294, 378, 527, 578
TscAI CASTG 4 cut(s) 28, 133, 241, 543
TseFI GTSAC 2 cut(s) 122, 701
TseI GCWGC 4 cut(s) 29, 397, 479, 671
Tsp45I GTSAC 2 cut(s) 122, 701
TspRI CASTG 4 cut(s) 28, 133, 241, 543
Tth111I GACNNNGTC 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.