AT1G10530

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009900
Physical Location & Seq
Reverse (-)
3471456 .. 3472674
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10530.1

Sequence Viewer

Length: 501 bp
ATGGGGAATTGTCAGGCGGTGAATGCGGCAGTGCTGGTTCTACAACATCCCGGCGGGATTATCGACCGGTACTATAGCTCTGTATCCGTCACCGAGGTCATGGCTATGTATCCTGGTCACTACGTCTCTCTCATCATTCCTTTGTCAGAGGAGGAAGAGAAGAATATTCCAGCGACGGAGAAGGGAGATGATAAGAAGCAGAGGAAGGCTGTGAGGTTTACACGTGTCCAGCTTCTCCGGCCAACGGAGAATCTTGTGCTCGGTCACGCTTACCGGCTCATTACTTCACAAGAAGTGATGAAGGTGTTACGGGAAAAGAAGAGCGCCAAGACGAAAAAACATCAGATTGAGAAGACAACCACGGCGAAGAAGTTCTCGGACAAGAAAGTTCCGGAGAAGAAACAAGGGAAGCAATTTCGTGTTATAAGGAATTCAACTTCCCTCTTAAAGTCTAAAACATGGAGGCCGTCTCTTCAGAGCATCTCCGAAGCTACTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

166

Amino Acids

18.81

Weight (kDa)

10.0

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 163 1.2e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 3 cut(s) 17, 26, 54
AcoI YGGCCR 1 cut(s) 239
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 458
AcvI CACGTG 1 cut(s) 224
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 244
AflIII ACRYGT 2 cut(s) 221, 223
AgeI ACCGGT 1 cut(s) 66
AgsI TTSAA 1 cut(s) 435
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 4 cut(s) 78, 232, 491, 498
AluI AGCT 4 cut(s) 78, 232, 491, 498
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 130, 474
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 2 cut(s) 239, 464
ApoI RAATTY 1 cut(s) 430
AsiGI ACCGGT 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 31, 82
BbrPI CACGTG 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 261
BceAI ACGGC 2 cut(s) 378, 451
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 2 cut(s) 94, 120
BcnI CCSGG 1 cut(s) 51
BcoDI GTCTC 2 cut(s) 130, 474
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 73
BfoI RGCGCY 1 cut(s) 327
BfuI GTATCC 2 cut(s) 94, 120
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 2 cut(s) 51, 114
BmrFI CCNGG 2 cut(s) 51, 114
BmsI GCATC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 359
BplI GAGNNNNNCTC 2 cut(s) 454, 486
BpuMI CCSGG 1 cut(s) 51
BsaAI YACGTR 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 93, 360
BsaWI WCCGGW 2 cut(s) 66, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse118I RCCGGY 2 cut(s) 66, 273
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 93, 360
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 164
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 2 cut(s) 241, 466
BshTI ACCGGT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 5 cut(s) 51, 67, 238, 274, 392
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 2 cut(s) 130, 474
BsmBI CGTCTC 2 cut(s) 130, 474
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 2 cut(s) 241, 466
Bsp1286I GDGCHC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 391
BspACI CCGC 3 cut(s) 17, 26, 54
BspANI GGCC 2 cut(s) 241, 466
BspEI TCCGGA 1 cut(s) 391
BspQI GCTCTTC 1 cut(s) 314
BsrFI RCCGGY 2 cut(s) 66, 273
BssAI RCCGGY 2 cut(s) 66, 273
BssECI CCNNGG 2 cut(s) 93, 360
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 3 cut(s) 150, 314, 477
BstBAI YACGTR 1 cut(s) 224
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 1 cut(s) 326
BstMAI GTCTC 2 cut(s) 130, 474
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 2 cut(s) 23, 238
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 2 cut(s) 49, 112
BstSFI CTRYAG 1 cut(s) 73
BstV2I GAAGAC 1 cut(s) 359
BsuI GTATCC 2 cut(s) 94, 120
BsuRI GGCC 2 cut(s) 241, 466
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
CfoI GCGC 1 cut(s) 326
Cfr10I RCCGGY 2 cut(s) 66, 273
Csp6I GTAC 1 cut(s) 70
CspAI ACCGGT 1 cut(s) 66
CviAII CATG 2 cut(s) 100, 459
CviJI RGCY 9 cut(s) 78, 104, 209, 232, 241, 277, 466, 491, 498
CviKI_1 RGCY 9 cut(s) 78, 104, 209, 232, 241, 277, 466, 491, 498
CviQI GTAC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 239
Eam1104I CTCTTC 3 cut(s) 150, 314, 477
EarI CTCTTC 3 cut(s) 150, 314, 477
Eco57I CTGAAG 1 cut(s) 458
Eco72I CACGTG 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 112
Esp3I CGTCTC 2 cut(s) 130, 474
FaeI CATG 2 cut(s) 103, 462
FaiI YATR 5 cut(s) 75, 101, 107, 425, 460
FatI CATG 2 cut(s) 99, 458
FauI CCCGC 1 cut(s) 47
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 33
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 1 cut(s) 495
GlaI GCGC 1 cut(s) 325
GluI GCNGC 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 241, 466
HapII CCGG 5 cut(s) 51, 67, 238, 274, 392
HhaI GCGC 1 cut(s) 326
Hin1II CATG 2 cut(s) 103, 462
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 1 cut(s) 250
HpaII CCGG 5 cut(s) 51, 67, 238, 274, 392
HphI GGTGA 2 cut(s) 31, 82
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 5 cut(s) 148, 345, 379, 477, 487
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 1 cut(s) 219
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 3 cut(s) 175, 199, 295
HpyCH4IV ACGT 2 cut(s) 123, 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 238
HpySE526I ACGT 2 cut(s) 123, 223
Hsp92II CATG 2 cut(s) 103, 462
HspAI GCGC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 314
LweI GCATC 1 cut(s) 489
MaeI CTAG 1 cut(s) 495
MaeII ACGT 2 cut(s) 123, 223
MaeIII GTNAC 4 cut(s) 88, 116, 263, 306
MboII GAAGA 7 cut(s) 167, 172, 331, 364, 379, 409, 464
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 3 cut(s) 7, 413, 430
MnlI CCTC 7 cut(s) 88, 142, 145, 195, 207, 452, 456
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 371
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 104
MspI CCGG 5 cut(s) 51, 67, 238, 274, 392
MspR9I CCNGG 2 cut(s) 51, 114
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 2 cut(s) 23, 238
NciI CCSGG 1 cut(s) 51
NlaIII CATG 2 cut(s) 103, 462
NmuCI GTSAC 3 cut(s) 88, 116, 263
PciSI GCTCTTC 1 cut(s) 314
PctI GAATGC 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 250
PinAI ACCGGT 1 cut(s) 66
PkrI GCNGC 1 cut(s) 28
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 224
PsiI TTATAA 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 112
PspCI CACGTG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 112
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 1 cut(s) 27
ScrFI CCNGG 2 cut(s) 51, 114
SduI GDGCHC 1 cut(s) 261
SetI ASST 9 cut(s) 80, 99, 126, 218, 226, 234, 306, 493, 500
SfaNI GCATC 1 cut(s) 489
SfcI CTRYAG 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 104
Sse9I AATT 3 cut(s) 7, 413, 430
SsiI CCGC 3 cut(s) 17, 26, 54
SspI AATATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 2 cut(s) 49, 112
TaiI ACGT 2 cut(s) 126, 226
TaqI TCGA 1 cut(s) 63
TaqII GACCGA 1 cut(s) 251
TasI AATT 3 cut(s) 7, 413, 430
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 3 cut(s) 88, 116, 263
Tsp45I GTSAC 3 cut(s) 88, 116, 263
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 3 cut(s) 76, 191, 260
TspRI CASTG 1 cut(s) 36
XapI RAATTY 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.