FvH4_4g34950

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009900
Physical Location & Seq
Forward (+)
32452846 .. 32454416
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34950.t1

Sequence Viewer

Length: 576 bp
ATGGGGAACTGTCAGGCCATTGATGCTGCGGCTCTGGTGATACAGCACCCGAGTGGGAAGATAGAGAGGCTGTACTGGCCTATTAGCGCCAGCGAGGTCATGAGGTTGAACCCTGGTCATTATGTTTCTTTGATTATCCCATTGCCGGCTTCGGAATCGGGTTCTGATCATCATCATCATAACCAAGAGGAGGAGATTCGGGAGCACAAGAGTAGTGGTGGTGGTGGTCCGGTGCGTTTCACAAGGGTGAAGCTTCTTCGGCCTAATGAGACTCTTGCTCTTGGCCATGCCTATCGCTTGGTCACTACACAAGAGGTTATGAAGGTGTTGAGGGCAAAGAAGTACGCGAAGACGAAGAAGCTGCAGGTGGAATCACTACTTGAGAGAACATCACAGACGGCAGTACCAGAGAAACAGAGTTCGAGTTGTGAGGCAGAGAACAAGGAAAATATCAACCATGCCACAAATCATGAAAGGCGCCGACCGAGGACGGCATCAGGGAATGCTGCTGGTGCTGCAATGAGGTCGAAATCTTGGCGGCCTTCATTGCAGAGCATTTCTGAAGCTGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

192

Amino Acids

21.1

Weight (kDa)

9.69

Isoelectric Point (pI)

60.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 188 1e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 355
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 477
AccII CGCG 1 cut(s) 347
AciI CCGC 2 cut(s) 29, 538
AcoI YGGCCR 1 cut(s) 283
AcyI GRCGYC 1 cut(s) 478
AfaI GTAC 3 cut(s) 74, 344, 405
AfiI CCNNNNNNNGG 2 cut(s) 145, 190
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 1 cut(s) 112
AleI CACNNNNGTG 2 cut(s) 51, 245
AluBI AGCT 4 cut(s) 253, 361, 566, 573
AluI AGCT 4 cut(s) 253, 361, 566, 573
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 5 cut(s) 15, 77, 260, 283, 539
ApeKI GCWGC 4 cut(s) 26, 361, 506, 515
AspLEI GCGC 2 cut(s) 89, 480
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 49, 259
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 227
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BalI TGGCCA 1 cut(s) 285
BanI GGYRCC 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 13, 348, 493, 502
BceAI ACGGC 2 cut(s) 414, 507
BcgI CGANNNNNNTGC 4 cut(s) 343, 377, 507, 541
BciT130I CCWGG 1 cut(s) 114
BclI TGATCA 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 362
BfoI RGCGCY 2 cut(s) 90, 481
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 6 cut(s) 27, 30, 362, 507, 516, 539
BlsI GCNGC 6 cut(s) 28, 31, 363, 508, 517, 540
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 13, 503
BpiI GAAGAC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 112, 485
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 190
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 80
Bse3DI GCAATG 3 cut(s) 140, 525, 545
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 112, 485
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 145, 190
BseMI GCAATG 3 cut(s) 140, 525, 545
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 2 cut(s) 203, 206
BseXI GCAGC 4 cut(s) 13, 348, 493, 502
BseYI CCCAGC 1 cut(s) 566
Bsh1236I CGCG 1 cut(s) 347
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 5 cut(s) 17, 79, 262, 285, 541
BshNI GGYRCC 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 146, 230
BslI CCNNNNNNNGG 2 cut(s) 145, 190
BsmAI GTCTC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 5 cut(s) 17, 79, 262, 285, 541
BsoBI CYCGRG 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 2 cut(s) 29, 538
BspANI GGCC 5 cut(s) 17, 79, 262, 285, 541
BspFNI CGCG 1 cut(s) 347
BspHI TCATGA 2 cut(s) 99, 469
BspLI GGNNCC 1 cut(s) 479
BspMAI CTGCAG 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 477
BsrDI GCAATG 3 cut(s) 140, 525, 545
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 112, 485
BssMI GATC 1 cut(s) 166
BssNI GRCGYC 1 cut(s) 478
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 478
BstC8I GCNNGC 2 cut(s) 91, 147
BstFNI CGCG 1 cut(s) 347
BstH2I RGCGCY 2 cut(s) 90, 481
BstHHI GCGC 2 cut(s) 89, 480
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 166
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 6 cut(s) 23, 76, 259, 512, 515, 547
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 1 cut(s) 362
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 4 cut(s) 13, 348, 493, 502
BstV2I GAAGAC 1 cut(s) 356
BsuRI GGCC 5 cut(s) 17, 79, 262, 285, 541
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 91, 147
CciI TCATGA 2 cut(s) 99, 469
CfoI GCGC 2 cut(s) 89, 480
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 3 cut(s) 73, 343, 404
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 4 cut(s) 100, 287, 458, 470
CviQI GTAC 3 cut(s) 73, 343, 404
DinI GGCGCC 1 cut(s) 479
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 112
EgeI GGCGCC 1 cut(s) 479
EheI GGCGCC 1 cut(s) 479
FaeI CATG 4 cut(s) 103, 290, 461, 473
FaiI YATR 7 cut(s) 101, 123, 180, 288, 320, 459, 471
FatI CATG 4 cut(s) 99, 286, 457, 469
FbaI TGATCA 1 cut(s) 166
Fnu4HI GCNGC 6 cut(s) 27, 30, 362, 507, 516, 539
Fsp4HI GCNGC 6 cut(s) 27, 30, 362, 507, 516, 539
GlaI GCGC 2 cut(s) 88, 479
GluI GCNGC 6 cut(s) 27, 30, 362, 507, 516, 539
GsaI CCCAGC 1 cut(s) 570
HaeII RGCGCY 2 cut(s) 90, 481
HaeIII GGCC 5 cut(s) 17, 79, 262, 285, 541
HapII CCGG 2 cut(s) 146, 230
HhaI GCGC 2 cut(s) 89, 480
Hin1I GRCGYC 1 cut(s) 478
Hin1II CATG 4 cut(s) 103, 290, 461, 473
Hin6I GCGC 2 cut(s) 87, 478
HinP1I GCGC 2 cut(s) 87, 478
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 4 cut(s) 155, 196, 271, 371
HpaII CCGG 2 cut(s) 146, 230
HphI GGTGA 2 cut(s) 49, 259
Hpy188I TCNGA 3 cut(s) 154, 166, 562
Hpy188III TCNNGA 3 cut(s) 100, 200, 470
HpyAV CCTTC 2 cut(s) 316, 552
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 364, 518, 550
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 76, 259, 512, 515, 547
Hsp92I GRCGYC 1 cut(s) 478
Hsp92II CATG 4 cut(s) 103, 290, 461, 473
HspAI GCGC 2 cut(s) 87, 478
KasI GGCGCC 1 cut(s) 477
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 2 cut(s) 202, 570
Lsp1109I GCAGC 4 cut(s) 13, 348, 493, 502
LweI GCATC 2 cut(s) 13, 503
MaeIII GTNAC 1 cut(s) 301
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 4 cut(s) 70, 248, 361, 367
MhlI GDGCHC 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 285
MluNI TGGCCA 1 cut(s) 285
Mly113I GGCGCC 1 cut(s) 478
MlyI GAGTC 1 cut(s) 265
Mox20I TGGCCA 1 cut(s) 285
MroNI GCCGGC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 285
MslI CAYNNNNRTG 2 cut(s) 51, 245
Msp20I TGGCCA 1 cut(s) 285
MspI CCGG 2 cut(s) 146, 230
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 6 cut(s) 23, 76, 259, 512, 515, 547
NaeI GCCGGC 1 cut(s) 147
NarI GGCGCC 1 cut(s) 478
NdeII GATC 1 cut(s) 166
NgoMIV GCCGGC 1 cut(s) 145
NlaIII CATG 4 cut(s) 103, 290, 461, 473
NlaIV GGNNCC 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 301
OliI CACNNNNGTG 2 cut(s) 51, 245
PagI TCATGA 2 cut(s) 99, 469
PaqCI CACCTGC 1 cut(s) 355
PctI GAATGC 1 cut(s) 508
PdiI GCCGGC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 155, 196, 371
PkrI GCNGC 6 cut(s) 28, 31, 363, 508, 517, 540
PleI GAGTC 1 cut(s) 265
PluTI GGCGCC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 1 cut(s) 227
PstI CTGCAG 1 cut(s) 366
RsaI GTAC 3 cut(s) 74, 344, 405
RsaNI GTAC 3 cut(s) 73, 343, 404
RseI CAYNNNNRTG 2 cut(s) 51, 245
SatI GCNGC 6 cut(s) 27, 30, 362, 507, 516, 539
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 2 cut(s) 13, 503
SfcI CTRYAG 1 cut(s) 362
SfoI GGCGCC 1 cut(s) 479
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 51, 245
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 2 cut(s) 29, 538
SspDI GGCGCC 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 422, 527
TaqII GACCGA 1 cut(s) 499
TatI WGTACW 1 cut(s) 72
TauI GCSGC 2 cut(s) 32, 541
TfiI GAWTC 3 cut(s) 155, 196, 371
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 4 cut(s) 26, 361, 506, 515
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 3 cut(s) 335, 486, 534
VpaK11BI GGWCC 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.