AT1G60010

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009900
Physical Location & Seq
Reverse (-)
22095285 .. 22096620
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60010.1

Sequence Viewer

Length: 522 bp
ATGGGAAATTGTCAAGCGGTTGATGCGGCTGCGTTGGTTCTACAACATCCTGATGGCAAGATCGATAGATATTACGGTCCTGTATCCGTTAGTGAGATCATGCGTATGTATCCTGGTCACTACGTTTCTCTTATTATTCCTTTGCCGGAGAAGAATATTCCGGCGACAACAACAACAACAGATGATAAGAGTGAGAGAAAGGTTGTGAGGTTCACGCGCGTGAAACTTCTCCGACCAACGGAGAATCTTGTACTTGGTCATGCTTATCGTCTCATCACTTCACAAGAGGTTATGAAGGTTTTAAGAGCCAAGAAGTACGCGAAAACAAAGAAACACCAAAGTGAAACGAGTAAGGAGAAGAAGAAGCCATCATCGGAGAAGAAGATTGATGAAGAATCCGACAAGAATCAGAATCTGGAAACGAAAGATGAGAAGCAACGTTCAGTGTTAACGAATTCAGCTTCATCGAGATCAAAAACATGGAGGCCATCATTGCAGAGCATCTCTGAAGCTACGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

173

Amino Acids

19.6

Weight (kDa)

9.71

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 170 9.6e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 217, 219, 320
AciI CCGC 2 cut(s) 17, 26
AclI AACGTT 1 cut(s) 439
AcsI RAATTY 1 cut(s) 454
AfaI GTAC 2 cut(s) 252, 317
AfiI CCNNNNNNNGG 1 cut(s) 238
AjnI CCWGG 1 cut(s) 112
AleI CACNNNNGTG 2 cut(s) 218, 339
AluBI AGCT 2 cut(s) 461, 512
AluI AGCT 2 cut(s) 461, 512
Alw26I GTCTC 1 cut(s) 275
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 1 cut(s) 485
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 454
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 77
AvaII GGWCC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 47, 376, 496
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 2 cut(s) 94, 120
BcoDI GTCTC 1 cut(s) 275
BfuI GTATCC 2 cut(s) 94, 120
BisI GCNGC 2 cut(s) 27, 30
BlsI GCNGC 2 cut(s) 28, 31
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 13, 510
Bsa29I ATCGAT 1 cut(s) 63
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 63
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 16
Bsh1236I CGCG 3 cut(s) 217, 219, 320
BshFI GGCC 1 cut(s) 487
BshVI ATCGAT 1 cut(s) 63
BsiSI CCGG 2 cut(s) 146, 161
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 1 cut(s) 275
BsmBI CGTCTC 1 cut(s) 275
BsnI GGCC 1 cut(s) 487
Bsp143I GATC 3 cut(s) 60, 96, 470
BspACI CCGC 2 cut(s) 17, 26
BspANI GGCC 1 cut(s) 487
BspDI ATCGAT 1 cut(s) 63
BspFNI CGCG 3 cut(s) 217, 219, 320
BsrDI GCAATG 1 cut(s) 491
BssMI GATC 3 cut(s) 60, 96, 470
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 77
BstF5I GGATG 1 cut(s) 46
BstFNI CGCG 3 cut(s) 217, 219, 320
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 3 cut(s) 63, 99, 473
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 3 cut(s) 60, 96, 470
BstMWI GCNNNNNNNGC 2 cut(s) 23, 493
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 3 cut(s) 217, 219, 320
BstV1I GCAGC 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 63
BsuI GTATCC 2 cut(s) 94, 120
BsuRI GGCC 1 cut(s) 487
BsuTUI ATCGAT 1 cut(s) 63
BtsCI GGATG 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 450
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 63
Csp6I GTAC 2 cut(s) 251, 316
CviAII CATG 3 cut(s) 100, 260, 480
CviJI RGCY 6 cut(s) 29, 308, 367, 461, 487, 512
CviKI_1 RGCY 6 cut(s) 29, 308, 367, 461, 487, 512
CviQI GTAC 2 cut(s) 251, 316
DpnI GATC 3 cut(s) 62, 98, 472
DpnII GATC 3 cut(s) 60, 96, 470
Eco47I GGWCC 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 3 cut(s) 103, 263, 483
FaiI YATR 5 cut(s) 101, 107, 261, 293, 481
FatI CATG 3 cut(s) 99, 259, 479
Fnu4HI GCNGC 2 cut(s) 27, 30
FokI GGATG 1 cut(s) 33
Fsp4HI GCNGC 2 cut(s) 27, 30
GlaI GCGC 1 cut(s) 218
GluI GCNGC 2 cut(s) 27, 30
HaeIII GGCC 1 cut(s) 487
HapII CCGG 2 cut(s) 146, 161
HhaI GCGC 1 cut(s) 219
Hin1II CATG 3 cut(s) 103, 263, 483
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 4 cut(s) 244, 395, 406, 412
HpaI GTTAAC 1 cut(s) 450
HpaII CCGG 2 cut(s) 146, 161
Hpy166II GTNNAC 2 cut(s) 213, 450
Hpy188I TCNGA 5 cut(s) 233, 376, 400, 411, 508
Hpy188III TCNNGA 3 cut(s) 50, 416, 468
Hpy8I GTNNAC 2 cut(s) 213, 450
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 123, 439
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 493
HpySE526I ACGT 2 cut(s) 123, 439
Hsp92II CATG 3 cut(s) 103, 263, 483
HspAI GCGC 1 cut(s) 217
KspAI GTTAAC 1 cut(s) 450
Kzo9I GATC 3 cut(s) 60, 96, 470
LpnPI CCDG 7 cut(s) 63, 93, 99, 126, 159, 174, 401
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 2 cut(s) 13, 510
MaeII ACGT 2 cut(s) 123, 439
MaeIII GTNAC 1 cut(s) 116
MalI GATC 3 cut(s) 62, 98, 472
MboI GATC 3 cut(s) 60, 96, 470
MboII GAAGA 6 cut(s) 163, 370, 373, 391, 394, 404
MluCI AATT 2 cut(s) 7, 454
MmeI TCCRAC 2 cut(s) 256, 423
MnlI CCTC 3 cut(s) 201, 280, 477
MseI TTAA 2 cut(s) 302, 449
MslI CAYNNNNRTG 4 cut(s) 51, 104, 218, 339
MspI CCGG 2 cut(s) 146, 161
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 3 cut(s) 217, 219, 320
MwoI GCNNNNNNNGC 2 cut(s) 23, 493
NdeII GATC 3 cut(s) 60, 96, 470
NlaIII CATG 3 cut(s) 103, 263, 483
NmuCI GTSAC 1 cut(s) 116
OliI CACNNNNGTG 2 cut(s) 218, 339
PfeI GAWTC 4 cut(s) 244, 395, 406, 412
PkrI GCNGC 2 cut(s) 28, 31
Psp1406I AACGTT 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 1 cut(s) 77
PstNI CAGNNNCTG 1 cut(s) 415
RsaI GTAC 2 cut(s) 252, 317
RsaNI GTAC 2 cut(s) 251, 316
RseI CAYNNNNRTG 4 cut(s) 51, 104, 218, 339
SaqAI TTAA 2 cut(s) 302, 449
SatI GCNGC 2 cut(s) 27, 30
Sau3AI GATC 3 cut(s) 60, 96, 470
Sau96I GGNCC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 114
SetI ASST 8 cut(s) 126, 204, 212, 291, 300, 442, 463, 514
SfaNI GCATC 2 cut(s) 13, 510
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 4 cut(s) 51, 104, 218, 339
Sse9I AATT 2 cut(s) 7, 454
SsiI CCGC 2 cut(s) 17, 26
SspI AATATT 1 cut(s) 157
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 126, 442
TaqI TCGA 2 cut(s) 63, 467
TasI AATT 2 cut(s) 7, 454
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 4 cut(s) 244, 395, 406, 412
Tru1I TTAA 2 cut(s) 302, 449
Tru9I TTAA 2 cut(s) 302, 449
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 308, 405, 453
TspGWI ACGGA 2 cut(s) 76, 254
TspRI CASTG 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.