MD13G1087400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009900
Physical Location & Seq
Reverse (-)
6123390 .. 6125066
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1087400.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGGGAACTGTCAAGCCATTGATGCAGCAGCTCTGGTGATACAGCATCCGAGTGGGAAGCTAGAGAGGATGTATTGGCCTATAAGTGCGAGTGAGGTGATGAGGTTGAACCCCGGTCACTACGTTTCCCTCATCATTCCGTTGCCGGTTTCCGAACAAGATCACGAACAAGTAGCAGAGAATCACGAGGAAGAGCACGGCAAAGCGGTGCGCTTCACTCGCGTCAAGCTTCTTCGGCCAAACGAAACTCTCGCTCTCGGCCATGCCTACCGGCTGGTTACTGCTCAAGAGGTTAAGAAGGTTTTGAAGGCGAAAAAGAAGAAACAGCAGCTCGAATTGCTCGAGAAGTTGCGAACTGGGCAAGGGAAGGAGAATTCGGGTTGCGAAGAGGCAGCAGCAGAAGTGAAATCTGATGAGAAGGAGAATAACAAGCATGCTTCAAAACGCGAAAGGCACCGATCAAGGACATCATCAGCGAATGCTGCCGCTGCTGCCGCTGCTGCTGCTGCCGCTGCTGCTGCTGCCGCACTGAGGTCCAAGTCTTGGCGCCCCTCGTTGCAGAGCATCTCCGAGGGCGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

195

Amino Acids

21.2

Weight (kDa)

9.46

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 191 2.1e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 453, 546
AccB7I CCANNNNNTGG 1 cut(s) 543
AccII CGCG 2 cut(s) 222, 447
AciI CCGC 5 cut(s) 206, 486, 495, 510, 525
AcoI YGGCCR 2 cut(s) 236, 259
AcsI RAATTY 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 547
AfiI CCNNNNNNNGG 2 cut(s) 531, 543
AgsI TTSAA 3 cut(s) 109, 307, 441
AluBI AGCT 4 cut(s) 32, 61, 229, 331
AluI AGCT 4 cut(s) 32, 61, 229, 331
Alw21I GWGCWC 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 3 cut(s) 77, 236, 259
ApoI RAATTY 1 cut(s) 373
AspLEI GCGC 2 cut(s) 213, 549
AspS9I GGNCC 1 cut(s) 534
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 49, 109
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 534
BanI GGYRCC 2 cut(s) 453, 546
BauI CACGAG 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 198
BceAI ACGGC 1 cut(s) 214
BcnI CCSGG 1 cut(s) 114
BfaI CTAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 550
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 534
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 534
BmiI GGNNCC 2 cut(s) 455, 548
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 3 cut(s) 13, 55, 573
BmuI ACTGGG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 547
BsaJI CCNNGG 2 cut(s) 112, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 543
Bse118I RCCGGY 2 cut(s) 145, 270
Bse1I ACTGG 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 112, 570
BseGI GGATG 2 cut(s) 46, 75
BseLI CCNNNNNNNGG 2 cut(s) 531, 543
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 361
Bsh1236I CGCG 2 cut(s) 222, 447
BshFI GGCC 3 cut(s) 79, 238, 261
BshNI GGYRCC 2 cut(s) 453, 546
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 3 cut(s) 114, 146, 271
BslI CCNNNNNNNGG 2 cut(s) 531, 543
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 3 cut(s) 79, 238, 261
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 160, 458
BspACI CCGC 5 cut(s) 206, 486, 495, 510, 525
BspANI GGCC 3 cut(s) 79, 238, 261
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 2 cut(s) 222, 447
BspLI GGNNCC 2 cut(s) 455, 548
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 2 cut(s) 453, 546
BsrFI RCCGGY 2 cut(s) 145, 270
BsrI ACTGG 1 cut(s) 361
BssAI RCCGGY 2 cut(s) 145, 270
BssECI CCNNGG 2 cut(s) 112, 570
BssMI GATC 2 cut(s) 160, 458
BssNI GRCGYC 1 cut(s) 547
BssSI CACGAG 1 cut(s) 185
Bst2BI CACGAG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 2 cut(s) 186, 381
BstACI GRCGYC 1 cut(s) 547
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 530
BstF5I GGATG 2 cut(s) 46, 75
BstFNI CGCG 2 cut(s) 222, 447
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 2 cut(s) 213, 549
BstKTI GATC 2 cut(s) 163, 461
BstMBI GATC 2 cut(s) 160, 458
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 1 cut(s) 112
BstUI CGCG 2 cut(s) 222, 447
BsuRI GGCC 3 cut(s) 79, 238, 261
BtsCI GGATG 2 cut(s) 46, 75
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 2 cut(s) 213, 549
Cfr10I RCCGGY 2 cut(s) 145, 270
Cfr13I GGNCC 1 cut(s) 534
CseI GACGC 1 cut(s) 211
CviAII CATG 2 cut(s) 263, 434
CviJI RGCY 9 cut(s) 17, 32, 61, 79, 229, 238, 261, 274, 331
CviKI_1 RGCY 9 cut(s) 17, 32, 61, 79, 229, 238, 261, 274, 331
DdeI CTNAG 1 cut(s) 530
DinI GGCGCC 1 cut(s) 548
DpnI GATC 2 cut(s) 162, 460
DpnII GATC 2 cut(s) 160, 458
EaeI YGGCCR 2 cut(s) 236, 259
Eam1104I CTCTTC 2 cut(s) 186, 381
EarI CTCTTC 2 cut(s) 186, 381
Eco47I GGWCC 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 373
EgeI GGCGCC 1 cut(s) 548
EheI GGCGCC 1 cut(s) 548
FaeI CATG 2 cut(s) 266, 437
FaiI YATR 3 cut(s) 83, 264, 435
FatI CATG 2 cut(s) 262, 433
FokI GGATG 2 cut(s) 33, 82
FspBI CTAG 1 cut(s) 62
GlaI GCGC 2 cut(s) 212, 548
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 3 cut(s) 79, 238, 261
HapII CCGG 3 cut(s) 114, 146, 271
HgaI GACGC 1 cut(s) 211
HhaI GCGC 2 cut(s) 213, 549
Hin1I GRCGYC 1 cut(s) 547
Hin1II CATG 2 cut(s) 266, 437
Hin6I GCGC 2 cut(s) 211, 547
HinP1I GCGC 2 cut(s) 211, 547
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 181
HpaII CCGG 3 cut(s) 114, 146, 271
HphI GGTGA 2 cut(s) 49, 109
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 4 cut(s) 51, 154, 412, 571
Hpy188III TCNNGA 4 cut(s) 164, 185, 287, 343
Hpy8I GTNNAC 1 cut(s) 580
HpyAV CCTTC 4 cut(s) 292, 301, 361, 412
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 26, 559
HpyF3I CTNAG 1 cut(s) 530
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 547
Hsp92II CATG 2 cut(s) 266, 437
HspAI GCGC 2 cut(s) 211, 547
KasI GGCGCC 1 cut(s) 546
Kzo9I GATC 2 cut(s) 160, 458
LguI GCTCTTC 1 cut(s) 186
LpnPI CCDG 6 cut(s) 20, 127, 159, 260, 284, 342
LweI GCATC 3 cut(s) 13, 55, 573
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 116, 277
MalI GATC 2 cut(s) 162, 460
MboI GATC 2 cut(s) 160, 458
MboII GAAGA 4 cut(s) 203, 224, 331, 398
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 2 cut(s) 335, 373
Mly113I GGCGCC 1 cut(s) 547
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 3 cut(s) 488, 497, 512
MspI CCGG 3 cut(s) 114, 146, 271
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 484
MvnI CGCG 2 cut(s) 222, 447
NarI GGCGCC 1 cut(s) 547
NciI CCSGG 1 cut(s) 114
NdeII GATC 2 cut(s) 160, 458
NlaIII CATG 2 cut(s) 266, 437
NlaIV GGNNCC 2 cut(s) 455, 548
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 437
PaeI GCATGC 1 cut(s) 437
PaeR7I CTCGAG 1 cut(s) 341
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 484
PfeI GAWTC 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 543
PluTI GGCGCC 1 cut(s) 550
PspN4I GGNNCC 2 cut(s) 455, 548
PspPI GGNCC 1 cut(s) 534
RseI CAYNNNNRTG 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 2 cut(s) 160, 458
Sau96I GGNCC 1 cut(s) 534
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 3 cut(s) 13, 55, 573
SfoI GGCGCC 1 cut(s) 548
Sfr274I CTCGAG 1 cut(s) 341
SinI GGWCC 1 cut(s) 534
SlaI CTCGAG 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 285, 341
SmoI CTYRAG 2 cut(s) 285, 341
SphI GCATGC 1 cut(s) 437
Sse9I AATT 2 cut(s) 335, 373
SsiI CCGC 5 cut(s) 206, 486, 495, 510, 525
SspDI GGCGCC 1 cut(s) 546
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 333, 342
TasI AATT 2 cut(s) 335, 373
TauI GCSGC 4 cut(s) 488, 497, 512, 527
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 116
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 1 cut(s) 534
Van91I CCANNNNNTGG 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 534
XapI RAATTY 1 cut(s) 373
XceI RCATGY 1 cut(s) 437
XhoI CTCGAG 1 cut(s) 341
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.