Prupe.1G258800_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009900
Physical Location & Seq
Forward (+)
26837753 .. 26840065
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G258800.1

Sequence Viewer

Length: 555 bp
ATGGGAAACTGTCAGGCTATTGATGCTGCAGCTCTGGTGGTACAGCATCCAAGTGGGAAGATAGAGAGGATGTATTGGCCTATCAGTGCAAGCGAGGTGATGAGATTGAACCCTGGTCACTATGTTTCTTTGATCATTCCATTGCCGGTTTCTGATCAACATCAGGAAGAGGTAGAGAGCCAGGAGCACAACAAGACTGTGCGTTTCACTCGTGTTAAGCTTCTTAGACCCAACGAAACTCTTGCTCTTGGCCATGCCTATCGGCTGGTCACAACTCAAGAGGTTGTAAAGGTGCTGAGGGCAAAGAAGTACGCAAAAACAAAGAAGCAGCAGCTGGAAACACTTGAGAAGTTGCAAGCAGGGCAAGAGAAACAGAGTTCAGGTTGTGATGAGGCAGAAGAAGGGAAGTCTGAGAAGGAGAATATCAACCATGCAACAAAACACGAAAGGCACCGACCCAGGACAGCGAATGCTGCTGCCGCAACTGCTGCTGCTGCACTGAGGTCAAAGTCTTGGCGCCCCTCATTGCAGAGCATCTCTGAGGGTGTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

185

Amino Acids

20.51

Weight (kDa)

9.37

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 450, 516
AciI CCGC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 517
AfaI GTAC 2 cut(s) 42, 311
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 3 cut(s) 112, 180, 458
AluBI AGCT 4 cut(s) 32, 220, 334, 552
AluI AGCT 4 cut(s) 32, 220, 334, 552
Alw21I GWGCWC 1 cut(s) 189
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 2 cut(s) 77, 250
ApeKI GCWGC 9 cut(s) 26, 29, 328, 331, 473, 476, 488, 491, 494
AspLEI GCGC 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 109
BalI TGGCCA 1 cut(s) 252
BanI GGYRCC 2 cut(s) 450, 516
BauI CACGAG 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 9 cut(s) 13, 41, 340, 343, 460, 463, 475, 478, 481
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 3 cut(s) 114, 182, 460
BclI TGATCA 2 cut(s) 132, 154
BfmI CTRYAG 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 520
Bme1390I CCNGG 3 cut(s) 114, 182, 460
BmiI GGNNCC 2 cut(s) 452, 518
BmrFI CCNGG 3 cut(s) 114, 182, 460
BmsI GCATC 3 cut(s) 13, 55, 543
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 2 cut(s) 261, 365
BsaBI GATNNNNATC 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 517
BsaJI CCNNGG 2 cut(s) 112, 458
Bse118I RCCGGY 1 cut(s) 145
Bse3DI GCAATG 2 cut(s) 140, 524
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 3 cut(s) 114, 182, 460
BseDI CCNNGG 2 cut(s) 112, 458
BseGI GGATG 2 cut(s) 46, 75
BseJI GATNNNNATC 1 cut(s) 159
BseMI GCAATG 2 cut(s) 140, 524
BseMII CTCAG 4 cut(s) 287, 402, 491, 531
BseXI GCAGC 9 cut(s) 13, 41, 340, 343, 460, 463, 475, 478, 481
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 2 cut(s) 79, 252
BshNI GGYRCC 2 cut(s) 450, 516
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 146
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 2 cut(s) 79, 252
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 2 cut(s) 132, 154
BspACI CCGC 1 cut(s) 480
BspANI GGCC 2 cut(s) 79, 252
BspCNI CTCAG 4 cut(s) 288, 403, 492, 532
BspLI GGNNCC 2 cut(s) 452, 518
BspMAI CTGCAG 1 cut(s) 31
BspT107I GGYRCC 2 cut(s) 450, 516
BsrDI GCAATG 2 cut(s) 140, 524
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 112, 458
BssMI GATC 2 cut(s) 132, 154
BssNI GRCGYC 1 cut(s) 517
BssSI CACGAG 1 cut(s) 210
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 3 cut(s) 114, 182, 460
Bst4CI ACNGT 2 cut(s) 11, 199
Bst6I CTCTTC 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 517
BstAPI GCANNNNNTGC 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 91, 357
BstDEI CTNAG 5 cut(s) 224, 296, 411, 500, 540
BstF5I GGATG 2 cut(s) 46, 75
BstH2I RGCGCY 1 cut(s) 520
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 2 cut(s) 135, 157
BstMBI GATC 2 cut(s) 132, 154
BstMWI GCNNNNNNNGC 7 cut(s) 23, 361, 473, 479, 485, 488, 494
BstNI CCWGG 3 cut(s) 114, 182, 460
BstSCI CCNGG 3 cut(s) 112, 180, 458
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 9 cut(s) 13, 41, 340, 343, 460, 463, 475, 478, 481
BsuRI GGCC 2 cut(s) 79, 252
BtsCI GGATG 2 cut(s) 46, 75
BtsIMutI CAGTG 2 cut(s) 91, 497
Cac8I GCNNGC 2 cut(s) 91, 357
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 1 cut(s) 519
Cfr10I RCCGGY 1 cut(s) 145
Csp6I GTAC 2 cut(s) 41, 310
CviAII CATG 2 cut(s) 254, 431
CviJI RGCY 9 cut(s) 17, 32, 79, 180, 220, 252, 265, 334, 552
CviKI_1 RGCY 9 cut(s) 17, 32, 79, 180, 220, 252, 265, 334, 552
CviQI GTAC 2 cut(s) 41, 310
DdeI CTNAG 5 cut(s) 224, 296, 411, 500, 540
DinI GGCGCC 1 cut(s) 518
DpnI GATC 2 cut(s) 134, 156
DpnII GATC 2 cut(s) 132, 154
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 112, 180, 458
EgeI GGCGCC 1 cut(s) 518
EheI GGCGCC 1 cut(s) 518
FaeI CATG 2 cut(s) 257, 434
FaiI YATR 3 cut(s) 123, 255, 432
FatI CATG 2 cut(s) 253, 430
FbaI TGATCA 2 cut(s) 132, 154
FokI GGATG 2 cut(s) 33, 82
GlaI GCGC 1 cut(s) 518
HaeII RGCGCY 1 cut(s) 520
HaeIII GGCC 2 cut(s) 79, 252
HapII CCGG 1 cut(s) 146
HhaI GCGC 1 cut(s) 519
Hin1I GRCGYC 1 cut(s) 517
Hin1II CATG 2 cut(s) 257, 434
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HindIII AAGCTT 1 cut(s) 218
HpaII CCGG 1 cut(s) 146
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 154, 412, 541
Hpy188III TCNNGA 2 cut(s) 164, 278
HpyAV CCTTC 2 cut(s) 395, 409
HpyCH4III ACNGT 2 cut(s) 11, 199
HpyCH4V TGCA 6 cut(s) 29, 89, 355, 434, 497, 529
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 361, 473, 479, 485, 488, 494
HpyF3I CTNAG 5 cut(s) 224, 296, 411, 500, 540
Hsp92I GRCGYC 1 cut(s) 517
Hsp92II CATG 2 cut(s) 257, 434
HspAI GCGC 1 cut(s) 517
KasI GGCGCC 1 cut(s) 516
Ksp22I TGATCA 2 cut(s) 132, 154
Kzo9I GATC 2 cut(s) 132, 154
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 9 cut(s) 13, 41, 340, 343, 460, 463, 475, 478, 481
LweI GCATC 3 cut(s) 13, 55, 543
MaeIII GTNAC 2 cut(s) 116, 268
MalI GATC 2 cut(s) 134, 156
MboI GATC 2 cut(s) 132, 154
MboII GAAGA 3 cut(s) 70, 179, 410
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 252
MluNI TGGCCA 1 cut(s) 252
Mly113I GGCGCC 1 cut(s) 517
MnlI CCTC 9 cut(s) 60, 88, 163, 274, 291, 385, 495, 532, 535
Mox20I TGGCCA 1 cut(s) 252
MscI TGGCCA 1 cut(s) 252
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 3 cut(s) 114, 182, 460
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 3 cut(s) 114, 182, 460
MwoI GCNNNNNNNGC 7 cut(s) 23, 361, 473, 479, 485, 488, 494
NarI GGCGCC 1 cut(s) 517
NdeII GATC 2 cut(s) 132, 154
NlaIII CATG 2 cut(s) 257, 434
NlaIV GGNNCC 2 cut(s) 452, 518
NmuCI GTSAC 2 cut(s) 116, 268
PctI GAATGC 1 cut(s) 475
PluTI GGCGCC 1 cut(s) 520
Psp6I CCWGG 3 cut(s) 112, 180, 458
PspGI CCWGG 3 cut(s) 112, 180, 458
PspN4I GGNNCC 2 cut(s) 452, 518
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 2 cut(s) 42, 311
RsaNI GTAC 2 cut(s) 41, 310
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 2 cut(s) 132, 154
ScrFI CCNGG 3 cut(s) 114, 182, 460
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 3 cut(s) 13, 55, 543
SfcI CTRYAG 1 cut(s) 27
SfoI GGCGCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 276, 344
SmoI CTYRAG 2 cut(s) 276, 344
SsiI CCGC 1 cut(s) 480
SspDI GGCGCC 1 cut(s) 516
StyD4I CCNGG 3 cut(s) 112, 180, 458
TaaI ACNGT 2 cut(s) 11, 199
TauI GCSGC 1 cut(s) 482
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 2 cut(s) 91, 504
TseFI GTSAC 2 cut(s) 116, 268
TseI GCWGC 9 cut(s) 26, 29, 328, 331, 473, 476, 488, 491, 494
Tsp45I GTSAC 2 cut(s) 116, 268
TspRI CASTG 2 cut(s) 91, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.