RLG00000005832

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009900
Physical Location & Seq
Reverse (-)
2214151 .. 2214926
776 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005832

Sequence Viewer

Length: 579 bp
ATGGGGAACTGTCAGGCTATTGATGCTGCTGCTCTGGTGATACAGCACCCGAGTGGGAAGATAGAGAGGCTGTACTGGACTATAAGCGCCAGCGAGGTCATGAGGTTGAACCCTGGTCATTACGTTTCTCTGATTATCCCATTGCCGGCTTCGGCTTCGGATTCGGATTCGGGTTCTGATCATCATCACCAGGAGGAGGAGATTCAGGAGCAGAAGAGTAGTGGCGGTGATGCGGTGCGTTTCACAAGGGTGAAGCTTCTTCGACCTAATGAGACTCTTGCTCTTGGTCATGCCTATCGTCTGGTCACTACTCAAGAGGTTATGAAGGTGTTGAGGGCAAAGAAGTATGCGAAGACGAAGAAGCAGCAGGTGGAATCACTAATTGAGAGATCATCACAGACGGCAGTGCCGGAGAAACAGAGTTCGAGTTGCGAGGCAGAGGACAAGGAAAATATCAACCATGCTACAAATCATGAAAGGCGCCGACCGAGGACGGCATCAGGGAATGCTGCTGGTGCTGCAATGAGGTCGAAATCTTGGCGGCCCTCATTGCAGAGCATTTCTGAAGCTGCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

193

Amino Acids

21.16

Weight (kDa)

9.12

Isoelectric Point (pI)

63.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 189 8.7e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 480
AciI CCGC 3 cut(s) 225, 233, 541
AcyI GRCGYC 1 cut(s) 481
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 145, 196
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 2 cut(s) 112, 189
AleI CACNNNNGTG 2 cut(s) 51, 248
AluBI AGCT 3 cut(s) 256, 569, 576
AluI AGCT 3 cut(s) 256, 569, 576
Alw26I GTCTC 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 6 cut(s) 26, 29, 364, 509, 518, 569
AspLEI GCGC 2 cut(s) 89, 483
AspS9I GGNCC 1 cut(s) 543
AsuHPI GGTGA 4 cut(s) 49, 179, 239, 262
AvaI CYCGRG 1 cut(s) 49
BanI GGYRCC 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 359
BbvI GCAGC 6 cut(s) 13, 16, 376, 496, 505, 556
BceAI ACGGC 2 cut(s) 417, 510
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 2 cut(s) 114, 191
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 266
BfoI RGCGCY 2 cut(s) 90, 484
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 7 cut(s) 27, 30, 365, 510, 519, 542, 570
BlsI GCNGC 7 cut(s) 28, 31, 366, 511, 520, 543, 571
Bme1390I CCNGG 2 cut(s) 114, 191
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 543
BmiI GGNNCC 1 cut(s) 482
BmrFI CCNGG 2 cut(s) 114, 191
BmsI GCATC 3 cut(s) 13, 220, 506
BpiI GAAGAC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 112, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 196
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 80
Bse3DI GCAATG 3 cut(s) 140, 528, 548
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 2 cut(s) 114, 191
BseDI CCNNGG 2 cut(s) 112, 488
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 145, 196
BseMI GCAATG 3 cut(s) 140, 528, 548
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 2 cut(s) 209, 212
BseXI GCAGC 6 cut(s) 13, 16, 376, 496, 505, 556
Bsh1285I CGRYCG 1 cut(s) 488
BshFI GGCC 1 cut(s) 544
BshNI GGYRCC 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 146, 410
BslI CCNNNNNNNGG 2 cut(s) 145, 196
BsmAI GTCTC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 49
Bsp143I GATC 2 cut(s) 178, 389
BspACI CCGC 3 cut(s) 225, 233, 541
BspANI GGCC 1 cut(s) 544
BspHI TCATGA 2 cut(s) 99, 472
BspLI GGNNCC 1 cut(s) 482
BspMI ACCTGC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 480
BsrDI GCAATG 3 cut(s) 140, 528, 548
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 112, 488
BssMI GATC 2 cut(s) 178, 389
BssNI GRCGYC 1 cut(s) 481
Bst2UI CCWGG 2 cut(s) 114, 191
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 91, 147, 574
BstH2I RGCGCY 2 cut(s) 90, 484
BstHHI GCGC 2 cut(s) 89, 483
BstKTI GATC 2 cut(s) 181, 392
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 178, 389
BstMCI CGRYCG 1 cut(s) 488
BstMWI GCNNNNNNNGC 4 cut(s) 23, 515, 518, 550
BstNI CCWGG 2 cut(s) 114, 191
BstSCI CCNGG 2 cut(s) 112, 189
BstV1I GCAGC 6 cut(s) 13, 16, 376, 496, 505, 556
BstV2I GAAGAC 1 cut(s) 359
BsuRI GGCC 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 3 cut(s) 91, 147, 574
CciI TCATGA 2 cut(s) 99, 472
CfoI GCGC 2 cut(s) 89, 483
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 543
Csp6I GTAC 1 cut(s) 73
CviAII CATG 4 cut(s) 100, 290, 461, 473
CviJI RGCY 8 cut(s) 17, 70, 149, 155, 256, 544, 569, 576
CviKI_1 RGCY 8 cut(s) 17, 70, 149, 155, 256, 544, 569, 576
CviQI GTAC 1 cut(s) 73
DinI GGCGCC 1 cut(s) 482
DpnI GATC 2 cut(s) 180, 391
DpnII GATC 2 cut(s) 178, 389
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 112, 189
EgeI GGCGCC 1 cut(s) 482
EheI GGCGCC 1 cut(s) 482
FaeI CATG 4 cut(s) 103, 293, 464, 476
FaiI YATR 7 cut(s) 83, 101, 291, 323, 348, 462, 474
FatI CATG 4 cut(s) 99, 289, 460, 472
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 7 cut(s) 27, 30, 365, 510, 519, 542, 570
Fsp4HI GCNGC 7 cut(s) 27, 30, 365, 510, 519, 542, 570
GlaI GCGC 2 cut(s) 88, 482
GluI GCNGC 7 cut(s) 27, 30, 365, 510, 519, 542, 570
HaeII RGCGCY 2 cut(s) 90, 484
HaeIII GGCC 1 cut(s) 544
HapII CCGG 2 cut(s) 146, 410
HhaI GCGC 2 cut(s) 89, 483
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 4 cut(s) 103, 293, 464, 476
Hin6I GCGC 2 cut(s) 87, 481
HinP1I GCGC 2 cut(s) 87, 481
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 5 cut(s) 161, 167, 202, 274, 374
HpaII CCGG 2 cut(s) 146, 410
HphI GGTGA 4 cut(s) 49, 179, 239, 262
Hpy188I TCNGA 5 cut(s) 132, 160, 166, 178, 565
Hpy188III TCNNGA 4 cut(s) 100, 206, 314, 473
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 521, 553, 572
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 515, 518, 550
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 4 cut(s) 103, 293, 464, 476
HspAI GCGC 2 cut(s) 87, 481
KasI GGCGCC 1 cut(s) 480
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 2 cut(s) 178, 389
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 6 cut(s) 13, 16, 376, 496, 505, 556
LweI GCATC 3 cut(s) 13, 220, 506
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 180, 391
MboI GATC 2 cut(s) 178, 389
MboII GAAGA 5 cut(s) 70, 226, 251, 364, 370
MluCI AATT 1 cut(s) 381
Mly113I GGCGCC 1 cut(s) 481
MlyI GAGTC 1 cut(s) 268
MroNI GCCGGC 1 cut(s) 145
MslI CAYNNNNRTG 2 cut(s) 51, 248
MspI CCGG 2 cut(s) 146, 410
MspR9I CCNGG 2 cut(s) 114, 191
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 2 cut(s) 114, 191
MwoI GCNNNNNNNGC 4 cut(s) 23, 515, 518, 550
NaeI GCCGGC 1 cut(s) 147
NarI GGCGCC 1 cut(s) 481
NdeII GATC 2 cut(s) 178, 389
NgoMIV GCCGGC 1 cut(s) 145
NlaIII CATG 4 cut(s) 103, 293, 464, 476
NlaIV GGNNCC 1 cut(s) 482
NmuCI GTSAC 1 cut(s) 304
OliI CACNNNNGTG 2 cut(s) 51, 248
PagI TCATGA 2 cut(s) 99, 472
PaqCI CACCTGC 1 cut(s) 358
PctI GAATGC 1 cut(s) 511
PdiI GCCGGC 1 cut(s) 147
PfeI GAWTC 4 cut(s) 161, 167, 202, 374
PkrI GCNGC 7 cut(s) 28, 31, 366, 511, 520, 543, 571
PleI GAGTC 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 268
Psp6I CCWGG 2 cut(s) 112, 189
PspGI CCWGG 2 cut(s) 112, 189
PspN4I GGNNCC 1 cut(s) 482
PspPI GGNCC 1 cut(s) 543
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 2 cut(s) 51, 248
SatI GCNGC 7 cut(s) 27, 30, 365, 510, 519, 542, 570
Sau3AI GATC 2 cut(s) 178, 389
Sau96I GGNCC 1 cut(s) 543
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 2 cut(s) 114, 191
SfaNI GCATC 3 cut(s) 13, 220, 506
SfoI GGCGCC 1 cut(s) 482
SmiMI CAYNNNNRTG 2 cut(s) 51, 248
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
Sse9I AATT 1 cut(s) 381
SsiI CCGC 3 cut(s) 225, 233, 541
SspDI GGCGCC 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 112, 189
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 262, 425, 530
TaqII GACCGA 1 cut(s) 502
TasI AATT 1 cut(s) 381
TatI WGTACW 1 cut(s) 72
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 4 cut(s) 161, 167, 202, 374
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 6 cut(s) 26, 29, 364, 509, 518, 569
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 338, 489
TspRI CASTG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.