Rroxscaffold_5G00384940

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009900
Physical Location & Seq
Forward (+)
64144709 .. 64145962
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384940.1

Sequence Viewer

Length: 573 bp
ATGGGGAACTGTCAGGCCATTGATGCTGCTGCTCTGGTGATACAGCACCCGAGTGGGAAGATAGAGAGGCTGTACTGGCCTATAAGCGCCAGCGAGGTCATGAGGTTGAACCCTGGTCATTACGTTTCTCTGATTATCCCATTGCCGGCTTCGGCTTCGGATTCGGGTTCTGATCATCATCACCAGGAGGAGGAGATTCAGGAGCAGAAGAGTATTAGTGGTGGTGCGGTGCGTTTCACAAGGGTGAAGCTTCTTCGACGTAATGAGACTCTTGCTCTTGGTCATGCCTATCGTCTGGTCACTACTCAAGAGGTTATGAAGGTGTTGAGGGCAAAGAAGTATGCGAAGACGAAGAAGCAGCAGGTGGAATCACTAATTGAGAGATCATCACAGATGGCAGTGCCGGAGAAACAGAGTTCGAGTTGTGAGGCAGAGGACAAGGAAAATATCAACCATGCTACAAATCATGAAAGGCGCCGACCCAGGACGGCATCAGGGAATGCTGCTGGTGCTGCAATGAGGTCGAAATCTTGGCGGCCCTCATTGCAGAGCATTTCTGAAGCTGCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

190

Amino Acids

21.04

Weight (kDa)

9.57

Isoelectric Point (pI)

65.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 187 1.2e-25 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 352
AccB1I GGYRCC 1 cut(s) 474
AciI CCGC 2 cut(s) 227, 535
AcyI GRCGYC 1 cut(s) 475
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 145, 190
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 3 cut(s) 112, 183, 482
AleI CACNNNNGTG 2 cut(s) 51, 242
AluBI AGCT 3 cut(s) 250, 563, 570
AluI AGCT 3 cut(s) 250, 563, 570
Alw26I GTCTC 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 3 cut(s) 15, 77, 536
ApeKI GCWGC 6 cut(s) 26, 29, 358, 503, 512, 563
AspLEI GCGC 2 cut(s) 89, 477
AspS9I GGNCC 1 cut(s) 537
AsuHPI GGTGA 3 cut(s) 49, 173, 256
AvaI CYCGRG 1 cut(s) 49
BanI GGYRCC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 353
BbvI GCAGC 6 cut(s) 13, 16, 370, 490, 499, 550
BccI CCATC 1 cut(s) 388
BceAI ACGGC 1 cut(s) 504
BcgI CGANNNNNNTGC 2 cut(s) 504, 538
BciT130I CCWGG 3 cut(s) 114, 185, 484
BclI TGATCA 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 260
BfoI RGCGCY 2 cut(s) 90, 478
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 7 cut(s) 27, 30, 359, 504, 513, 536, 564
BlsI GCNGC 7 cut(s) 28, 31, 360, 505, 514, 537, 565
Bme1390I CCNGG 3 cut(s) 114, 185, 484
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 1 cut(s) 476
BmrFI CCNGG 3 cut(s) 114, 185, 484
BmsI GCATC 2 cut(s) 13, 500
BpiI GAAGAC 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 112, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 190
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 80
Bse3DI GCAATG 3 cut(s) 140, 522, 542
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 3 cut(s) 114, 185, 484
BseDI CCNNGG 2 cut(s) 112, 482
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 145, 190
BseMI GCAATG 3 cut(s) 140, 522, 542
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 2 cut(s) 203, 206
BseXI GCAGC 6 cut(s) 13, 16, 370, 490, 499, 550
BshFI GGCC 3 cut(s) 17, 79, 538
BshNI GGYRCC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 146, 404
BslI CCNNNNNNNGG 2 cut(s) 145, 190
BsmAI GTCTC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 3 cut(s) 17, 79, 538
BsoBI CYCGRG 1 cut(s) 49
Bsp143I GATC 2 cut(s) 172, 383
BspACI CCGC 2 cut(s) 227, 535
BspANI GGCC 3 cut(s) 17, 79, 538
BspHI TCATGA 2 cut(s) 99, 466
BspLI GGNNCC 1 cut(s) 476
BspMI ACCTGC 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 474
BsrDI GCAATG 3 cut(s) 140, 522, 542
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 112, 482
BssMI GATC 2 cut(s) 172, 383
BssNI GRCGYC 1 cut(s) 475
Bst2UI CCWGG 3 cut(s) 114, 185, 484
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 475
BstC8I GCNNGC 3 cut(s) 91, 147, 568
BstH2I RGCGCY 2 cut(s) 90, 478
BstHHI GCGC 2 cut(s) 89, 477
BstKTI GATC 2 cut(s) 175, 386
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 2 cut(s) 172, 383
BstMWI GCNNNNNNNGC 5 cut(s) 23, 76, 509, 512, 544
BstNI CCWGG 3 cut(s) 114, 185, 484
BstSCI CCNGG 3 cut(s) 112, 183, 482
BstV1I GCAGC 6 cut(s) 13, 16, 370, 490, 499, 550
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 3 cut(s) 17, 79, 538
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 3 cut(s) 91, 147, 568
CciI TCATGA 2 cut(s) 99, 466
CfoI GCGC 2 cut(s) 89, 477
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 1 cut(s) 73
CviAII CATG 4 cut(s) 100, 284, 455, 467
CviJI RGCY 9 cut(s) 17, 70, 79, 149, 155, 250, 538, 563, 570
CviKI_1 RGCY 9 cut(s) 17, 70, 79, 149, 155, 250, 538, 563, 570
CviQI GTAC 1 cut(s) 73
DinI GGCGCC 1 cut(s) 476
DpnI GATC 2 cut(s) 174, 385
DpnII GATC 2 cut(s) 172, 383
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco88I CYCGRG 1 cut(s) 49
EcoRII CCWGG 3 cut(s) 112, 183, 482
EgeI GGCGCC 1 cut(s) 476
EheI GGCGCC 1 cut(s) 476
FaeI CATG 4 cut(s) 103, 287, 458, 470
FaiI YATR 7 cut(s) 83, 101, 285, 317, 342, 456, 468
FatI CATG 4 cut(s) 99, 283, 454, 466
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 7 cut(s) 27, 30, 359, 504, 513, 536, 564
Fsp4HI GCNGC 7 cut(s) 27, 30, 359, 504, 513, 536, 564
GlaI GCGC 2 cut(s) 88, 476
GluI GCNGC 7 cut(s) 27, 30, 359, 504, 513, 536, 564
HaeII RGCGCY 2 cut(s) 90, 478
HaeIII GGCC 3 cut(s) 17, 79, 538
HapII CCGG 2 cut(s) 146, 404
HhaI GCGC 2 cut(s) 89, 477
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 4 cut(s) 103, 287, 458, 470
Hin6I GCGC 2 cut(s) 87, 475
HinP1I GCGC 2 cut(s) 87, 475
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 4 cut(s) 161, 196, 268, 368
HpaII CCGG 2 cut(s) 146, 404
HphI GGTGA 3 cut(s) 49, 173, 256
Hpy188I TCNGA 4 cut(s) 132, 160, 172, 559
Hpy188III TCNNGA 4 cut(s) 100, 200, 308, 467
Hpy99I CGWCG 1 cut(s) 261
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 2 cut(s) 123, 259
HpyCH4V TGCA 3 cut(s) 515, 547, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 76, 509, 512, 544
HpySE526I ACGT 2 cut(s) 123, 259
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 4 cut(s) 103, 287, 458, 470
HspAI GCGC 2 cut(s) 87, 475
KasI GGCGCC 1 cut(s) 474
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 2 cut(s) 172, 383
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 6 cut(s) 13, 16, 370, 490, 499, 550
LweI GCATC 2 cut(s) 13, 500
MaeII ACGT 2 cut(s) 123, 259
MaeIII GTNAC 1 cut(s) 298
MalI GATC 2 cut(s) 174, 385
MboI GATC 2 cut(s) 172, 383
MboII GAAGA 5 cut(s) 70, 220, 245, 358, 364
MluCI AATT 1 cut(s) 375
Mly113I GGCGCC 1 cut(s) 475
MlyI GAGTC 1 cut(s) 262
MroNI GCCGGC 1 cut(s) 145
MslI CAYNNNNRTG 2 cut(s) 51, 242
MspI CCGG 2 cut(s) 146, 404
MspR9I CCNGG 3 cut(s) 114, 185, 484
Mva1269I GAATGC 1 cut(s) 505
MvaI CCWGG 3 cut(s) 114, 185, 484
MwoI GCNNNNNNNGC 5 cut(s) 23, 76, 509, 512, 544
NaeI GCCGGC 1 cut(s) 147
NarI GGCGCC 1 cut(s) 475
NdeII GATC 2 cut(s) 172, 383
NgoMIV GCCGGC 1 cut(s) 145
NlaIII CATG 4 cut(s) 103, 287, 458, 470
NlaIV GGNNCC 1 cut(s) 476
NmuCI GTSAC 1 cut(s) 298
OliI CACNNNNGTG 2 cut(s) 51, 242
PagI TCATGA 2 cut(s) 99, 466
PaqCI CACCTGC 1 cut(s) 352
PctI GAATGC 1 cut(s) 505
PdiI GCCGGC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 161, 196, 368
PkrI GCNGC 7 cut(s) 28, 31, 360, 505, 514, 537, 565
PleI GAGTC 1 cut(s) 262
PluTI GGCGCC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 262
Psp6I CCWGG 3 cut(s) 112, 183, 482
PspGI CCWGG 3 cut(s) 112, 183, 482
PspN4I GGNNCC 1 cut(s) 476
PspPI GGNCC 1 cut(s) 537
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 2 cut(s) 51, 242
SatI GCNGC 7 cut(s) 27, 30, 359, 504, 513, 536, 564
Sau3AI GATC 2 cut(s) 172, 383
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 3 cut(s) 114, 185, 484
SfaNI GCATC 2 cut(s) 13, 500
SfoI GGCGCC 1 cut(s) 476
SmiMI CAYNNNNRTG 2 cut(s) 51, 242
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
Sse9I AATT 1 cut(s) 375
SsiI CCGC 2 cut(s) 227, 535
SspDI GGCGCC 1 cut(s) 474
StyD4I CCNGG 3 cut(s) 112, 183, 482
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 2 cut(s) 126, 262
TaqI TCGA 3 cut(s) 256, 419, 524
TasI AATT 1 cut(s) 375
TatI WGTACW 1 cut(s) 72
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 3 cut(s) 161, 196, 368
TscAI CASTG 1 cut(s) 405
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 6 cut(s) 26, 29, 358, 503, 512, 563
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 2 cut(s) 332, 483
TspRI CASTG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.