Rorug04G0352800

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009900
Physical Location & Seq
Reverse (-)
52483714 .. 52486668
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0352800.1

Sequence Viewer

Length: 861 bp
ATGTCATTTACAATCTGCCAACCCCATGCGCACCTTAGCCCACCCCTCCCTCCCATGTCTATCCTACGCCGCCTCAACCCCTCCCACCTAACCCTCCACCGCCGCCTCGCCACCGTCGCAGCAGCCGTACGCCAAGACACCACTCTCTGGACCCCGGCCCCGCTCTCCCGGGTTGAGCCGGCCGCCGAGTCCCTCTTCCACGTCCGCGTCGACCTCTCCGACGCGCCCGACCTCGCTTCCTCCTACACGCGCGCCGGGCAGTACCTCCAGCTCCGCGTCCCGGACGTGGAGAAGCCCTCGTTCTTGGCCATCGCGTCGCCGCCGGCCCTGGCGGCGAAGGGCGTGTTCGAGTTCTTGGTGAAGAGCGTGGAGGGGTCCATCGCCGAGCTGCTGTGCGGGCTGAAGAAGGGGGACGTTGTGGAGCTGACCCAGGCCATGGGGAAAGGCTTCGAAATCGATCGGATCGAGCCGCCGGAGAATTATCCAACGGTTCTAATTTTCGCCACGGGATCTGGAATCAGTCCAATCAGGTCTCTGATTGAATCAGGGTTTAACGCCGACAAGCGATCCGACGTGAAGGTCTTCTATGGGGCCAGAAACCTTGACAGAATGGCTTATCAGGATAGATTTAAAGATTGGGAAGCTTCAGGTGTAAAGATAGTTCCTGTATTGTCACAACCACACGACGGCTGGACTGGACAAAGTGGTTATGTGCAGGCTGCTTTTACTAGAGCCAAGCAAGTTTATAACCCTCTCTCCACCGCTGCTGTTCTTTGTGGGCAGAAACAGATGGCTGAGGAAGTTACCTCAATTCTTTTAGCAGATGGAGTTGCAAGTGAAAAAATACTGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

286

Amino Acids

31.2

Weight (kDa)

7.74

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 168 - 268 2.8e-16 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AasI GACNNNNNNGTC 1 cut(s) 578
Acc16I TGCGCA 1 cut(s) 30
AccB7I CCANNNNNTGG 2 cut(s) 147, 436
AccBSI CCGCTC 1 cut(s) 163
AccI GTMKAC 1 cut(s) 210
AccII CGCG 6 cut(s) 207, 224, 250, 252, 276, 314
AclWI GGATC 3 cut(s) 470, 517, 561
AcoI YGGCCR 2 cut(s) 180, 306
AcsI RAATTY 1 cut(s) 853
AcuI CTGAAG 2 cut(s) 422, 630
AfaI GTAC 2 cut(s) 129, 263
AfiI CCNNNNNNNGG 5 cut(s) 147, 280, 286, 436, 686
AgsI TTSAA 1 cut(s) 542
AjiI CACGTC 3 cut(s) 202, 286, 574
AjnI CCWGG 2 cut(s) 327, 429
AluBI AGCT 4 cut(s) 271, 388, 424, 644
AluI AGCT 4 cut(s) 271, 388, 424, 644
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 3 cut(s) 470, 517, 561
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 6 cut(s) 156, 180, 306, 324, 432, 591
ApeKI GCWGC 5 cut(s) 119, 122, 388, 719, 764
ApoI RAATTY 1 cut(s) 853
Asp700I GAANNNNTTC 3 cut(s) 446, 581, 854
AspLEI GCGC 4 cut(s) 31, 226, 252, 254
AspS9I GGNCC 5 cut(s) 150, 157, 325, 375, 591
AsuC2I CCSGG 5 cut(s) 155, 169, 170, 256, 281
AsuHPI GGTGA 1 cut(s) 370
AsuII TTCGAA 1 cut(s) 450
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 2 cut(s) 150, 375
BalI TGGCCA 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 574
BbvCI CCTCAGC 1 cut(s) 795
BbvI GCAGC 5 cut(s) 131, 134, 375, 706, 751
BccI CCATC 4 cut(s) 317, 386, 784, 818
BceAI ACGGC 2 cut(s) 110, 703
BciT130I CCWGG 2 cut(s) 329, 431
BcnI CCSGG 5 cut(s) 155, 169, 170, 256, 281
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 1 cut(s) 729
BglI GCCNNNNNGGC 1 cut(s) 332
Bme1390I CCNGG 7 cut(s) 155, 169, 170, 256, 281, 329, 431
Bme18I GGWCC 2 cut(s) 150, 375
BmeT110I CYCGRG 1 cut(s) 168
BmgBI CACGTC 3 cut(s) 202, 286, 574
BmgT120I GGNCC 5 cut(s) 150, 157, 325, 375, 591
BmiI GGNNCC 4 cut(s) 152, 159, 376, 592
BmrFI CCNGG 7 cut(s) 155, 169, 170, 256, 281, 329, 431
BpiI GAAGAC 1 cut(s) 574
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 35, 795
Bpu14I TTCGAA 1 cut(s) 450
BpuMI CCSGG 5 cut(s) 155, 169, 170, 256, 281
Bsa29I ATCGAT 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 6 cut(s) 153, 168, 327, 429, 435, 504
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 280, 286, 436, 686
Bse118I RCCGGY 2 cut(s) 178, 322
Bse1I ACTGG 1 cut(s) 700
BseBI CCWGG 2 cut(s) 329, 431
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 6 cut(s) 153, 168, 327, 429, 435, 504
BseLI CCNNNNNNNGG 5 cut(s) 147, 280, 286, 436, 686
BseMII CTCAG 1 cut(s) 786
BseNI ACTGG 1 cut(s) 700
BsePI GCGCGC 1 cut(s) 250
BseX3I CGGCCG 1 cut(s) 180
BseXI GCAGC 5 cut(s) 131, 134, 375, 706, 751
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 6 cut(s) 207, 224, 250, 252, 276, 314
Bsh1285I CGRYCG 2 cut(s) 183, 460
BshFI GGCC 6 cut(s) 158, 182, 308, 326, 434, 593
BshVI ATCGAT 1 cut(s) 456
BsiEI CGRYCG 2 cut(s) 183, 460
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 7 cut(s) 155, 169, 179, 255, 281, 323, 473
BsiWI CGTACG 1 cut(s) 127
BslFI GGGAC 3 cut(s) 175, 263, 425
BslI CCNNNNNNNGG 5 cut(s) 147, 280, 286, 436, 686
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 3 cut(s) 175, 263, 425
BsnI GGCC 6 cut(s) 158, 182, 308, 326, 434, 593
Bso31I GGTCTC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 450
Bsp143I GATC 4 cut(s) 457, 462, 509, 566
Bsp19I CCATGG 1 cut(s) 435
BspANI GGCC 6 cut(s) 158, 182, 308, 326, 434, 593
BspCNI CTCAG 1 cut(s) 787
BspDI ATCGAT 1 cut(s) 456
BspFNI CGCG 6 cut(s) 207, 224, 250, 252, 276, 314
BspLI GGNNCC 4 cut(s) 152, 159, 376, 592
BspPI GGATC 3 cut(s) 470, 517, 561
BspQI GCTCTTC 1 cut(s) 356
BspT104I TTCGAA 1 cut(s) 450
BspTNI GGTCTC 1 cut(s) 537
BsrBI CCGCTC 1 cut(s) 163
BsrFI RCCGGY 2 cut(s) 178, 322
BsrI ACTGG 1 cut(s) 700
BssAI RCCGGY 2 cut(s) 178, 322
BssECI CCNNGG 6 cut(s) 153, 168, 327, 429, 435, 504
BssHII GCGCGC 1 cut(s) 250
BssMI GATC 4 cut(s) 457, 462, 509, 566
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 329, 431
Bst4CI ACNGT 2 cut(s) 115, 490
Bst6I CTCTTC 2 cut(s) 200, 356
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 5 cut(s) 180, 252, 324, 398, 717
BstDEI CTNAG 2 cut(s) 35, 795
BstDSI CCRYGG 2 cut(s) 435, 504
BstFNI CGCG 6 cut(s) 207, 224, 250, 252, 276, 314
BstHHI GCGC 4 cut(s) 31, 226, 252, 254
BstKTI GATC 4 cut(s) 460, 465, 512, 569
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 4 cut(s) 457, 462, 509, 566
BstMCI CGRYCG 2 cut(s) 183, 460
BstMWI GCNNNNNNNGC 4 cut(s) 116, 256, 332, 397
BstNI CCWGG 2 cut(s) 329, 431
BstSCI CCNGG 7 cut(s) 153, 167, 168, 254, 279, 327, 429
BstUI CGCG 6 cut(s) 207, 224, 250, 252, 276, 314
BstV1I GCAGC 5 cut(s) 131, 134, 375, 706, 751
BstV2I GAAGAC 1 cut(s) 574
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BstZI CGGCCG 1 cut(s) 180
Bsu15I ATCGAT 1 cut(s) 456
BsuRI GGCC 6 cut(s) 158, 182, 308, 326, 434, 593
BsuTUI ATCGAT 1 cut(s) 456
BtgI CCRYGG 2 cut(s) 435, 504
BtgZI GCGATG 2 cut(s) 295, 364
BtrI CACGTC 3 cut(s) 202, 286, 574
Cac8I GCNNGC 5 cut(s) 180, 252, 324, 398, 717
CfoI GCGC 4 cut(s) 31, 226, 252, 254
Cfr10I RCCGGY 2 cut(s) 178, 322
Cfr13I GGNCC 5 cut(s) 150, 157, 325, 375, 591
Cfr9I CCCGGG 1 cut(s) 168
ClaI ATCGAT 1 cut(s) 456
CseI GACGC 4 cut(s) 196, 230, 265, 303
Csp6I GTAC 2 cut(s) 128, 262
CviAII CATG 3 cut(s) 26, 55, 436
CviQI GTAC 2 cut(s) 128, 262
DdeI CTNAG 2 cut(s) 35, 795
DpnI GATC 4 cut(s) 459, 464, 511, 568
DpnII GATC 4 cut(s) 457, 462, 509, 566
DraI TTTAAA 1 cut(s) 631
DrdI GACNNNNNNGTC 1 cut(s) 578
DseDI GACNNNNNNGTC 1 cut(s) 578
EaeI YGGCCR 2 cut(s) 180, 306
EagI CGGCCG 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 200, 356
EarI CTCTTC 2 cut(s) 200, 356
EclXI CGGCCG 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 150, 375
Eco52I CGGCCG 1 cut(s) 180
Eco57I CTGAAG 2 cut(s) 422, 630
Eco88I CYCGRG 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 327, 429
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 3 cut(s) 29, 58, 439
FaiI YATR 6 cut(s) 27, 56, 437, 588, 711, 747
FaqI GGGAC 3 cut(s) 175, 263, 425
FatI CATG 3 cut(s) 25, 54, 435
FauI CCCGC 2 cut(s) 168, 389
FblI GTMKAC 1 cut(s) 210
FspAI RTGCGCAY 1 cut(s) 30
FspBI CTAG 1 cut(s) 729
FspI TGCGCA 1 cut(s) 30
GlaI GCGC 4 cut(s) 30, 225, 251, 253
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 6 cut(s) 158, 182, 308, 326, 434, 593
HapII CCGG 7 cut(s) 155, 169, 179, 255, 281, 323, 473
HgaI GACGC 4 cut(s) 196, 230, 265, 303
HhaI GCGC 4 cut(s) 31, 226, 252, 254
Hin1II CATG 3 cut(s) 29, 58, 439
Hin6I GCGC 4 cut(s) 29, 224, 250, 252
HinP1I GCGC 4 cut(s) 29, 224, 250, 252
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 3 cut(s) 188, 516, 542
HpaII CCGG 7 cut(s) 155, 169, 179, 255, 281, 323, 473
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 4 cut(s) 220, 462, 537, 571
Hpy188III TCNNGA 3 cut(s) 148, 513, 620
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 6 cut(s) 119, 212, 224, 319, 575, 689
HpyAV CCTTC 3 cut(s) 331, 400, 571
HpyCH4III ACNGT 2 cut(s) 115, 490
HpyCH4IV ACGT 4 cut(s) 201, 285, 414, 573
HpyCH4V TGCA 2 cut(s) 715, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 256, 332, 397
HpyF3I CTNAG 2 cut(s) 35, 795
HpySE526I ACGT 4 cut(s) 201, 285, 414, 573
Hsp92II CATG 3 cut(s) 29, 58, 439
HspAI GCGC 4 cut(s) 29, 224, 250, 252
KroI GCCGGC 2 cut(s) 178, 322
KroNI GCCGGC 2 cut(s) 180, 324
Kzo9I GATC 4 cut(s) 457, 462, 509, 566
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 276, 421
Lsp1109I GCAGC 5 cut(s) 131, 134, 375, 706, 751
MaeI CTAG 1 cut(s) 729
MaeII ACGT 4 cut(s) 201, 285, 414, 573
MaeIII GTNAC 2 cut(s) 672, 802
MalI GATC 4 cut(s) 459, 464, 511, 568
MbiI CCGCTC 1 cut(s) 163
MboI GATC 4 cut(s) 457, 462, 509, 566
MboII GAAGA 4 cut(s) 187, 373, 415, 574
MflI RGATCY 1 cut(s) 509
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 4 cut(s) 478, 495, 810, 853
MluNI TGGCCA 1 cut(s) 308
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 3 cut(s) 243, 509, 594
Mox20I TGGCCA 1 cut(s) 308
MroNI GCCGGC 2 cut(s) 178, 322
MroXI GAANNNNTTC 3 cut(s) 446, 581, 854
MscI TGGCCA 1 cut(s) 308
MseI TTAA 2 cut(s) 552, 630
Msp20I TGGCCA 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 7 cut(s) 155, 169, 179, 255, 281, 323, 473
MspR9I CCNGG 7 cut(s) 155, 169, 170, 256, 281, 329, 431
MvaI CCWGG 2 cut(s) 329, 431
MvnI CGCG 6 cut(s) 207, 224, 250, 252, 276, 314
MwoI GCNNNNNNNGC 4 cut(s) 116, 256, 332, 397
NaeI GCCGGC 2 cut(s) 180, 324
NciI CCSGG 5 cut(s) 155, 169, 170, 256, 281
NcoI CCATGG 1 cut(s) 435
NdeII GATC 4 cut(s) 457, 462, 509, 566
NgoMIV GCCGGC 2 cut(s) 178, 322
NlaIII CATG 3 cut(s) 29, 58, 439
NlaIV GGNNCC 4 cut(s) 152, 159, 376, 592
NmeAIII GCCGAG 2 cut(s) 211, 409
NmuCI GTSAC 1 cut(s) 672
NsbI TGCGCA 1 cut(s) 30
NspV TTCGAA 1 cut(s) 450
PauI GCGCGC 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 4 cut(s) 123, 207, 216, 462
PdiI GCCGGC 2 cut(s) 180, 324
PdmI GAANNNNTTC 3 cut(s) 446, 581, 854
PfeI GAWTC 2 cut(s) 516, 542
Pfl23II CGTACG 1 cut(s) 127
PflMI CCANNNNNTGG 2 cut(s) 147, 436
PfoI TCCNGGA 1 cut(s) 279
Ple19I CGATCG 1 cut(s) 460
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PsiI TTATAA 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 327, 429
PspGI CCWGG 2 cut(s) 327, 429
PspLI CGTACG 1 cut(s) 127
PspN4I GGNNCC 4 cut(s) 152, 159, 376, 592
PspPI GGNCC 5 cut(s) 150, 157, 325, 375, 591
PsrI GAACNNNNNNTAC 2 cut(s) 645, 677
PsuI RGATCY 1 cut(s) 509
PteI GCGCGC 1 cut(s) 250
PvuI CGATCG 1 cut(s) 460
RsaI GTAC 2 cut(s) 129, 263
RsaNI GTAC 2 cut(s) 128, 262
SalI GTCGAC 1 cut(s) 209
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 552, 630
Sau3AI GATC 4 cut(s) 457, 462, 509, 566
Sau96I GGNCC 5 cut(s) 150, 157, 325, 375, 591
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 7 cut(s) 155, 169, 170, 256, 281, 329, 431
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 2 cut(s) 150, 375
SmaI CCCGGG 1 cut(s) 170
Sse9I AATT 4 cut(s) 478, 495, 810, 853
SspMI CTAG 1 cut(s) 729
StyD4I CCNGG 7 cut(s) 153, 167, 168, 254, 279, 327, 429
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 115, 490
TaiI ACGT 4 cut(s) 204, 288, 417, 576
TaqI TCGA 5 cut(s) 210, 348, 450, 456, 465
TasI AATT 4 cut(s) 478, 495, 810, 853
TauI GCSGC 6 cut(s) 72, 105, 185, 322, 335, 472
TfiI GAWTC 2 cut(s) 516, 542
Tru1I TTAA 2 cut(s) 552, 630
Tru9I TTAA 2 cut(s) 552, 630
TseFI GTSAC 1 cut(s) 672
TseI GCWGC 5 cut(s) 119, 122, 388, 719, 764
Tsp45I GTSAC 1 cut(s) 672
TspMI CCCGGG 1 cut(s) 168
Van91I CCANNNNNTGG 2 cut(s) 147, 436
VpaK11BI GGWCC 2 cut(s) 150, 375
XapI RAATTY 1 cut(s) 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 687
XmaI CCCGGG 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 3 cut(s) 446, 581, 854
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.