MD16G1087900.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009900
Physical Location & Seq
Reverse (-)
6060805 .. 6062481
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1087900.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGGGAACTGTCAAGCCATTGATGCAGCAGCTCTGGTGATACAGCATCCGAGTGGGAAGATAGAGAGGATGTATTGGCCTATAAGTGCGAGTGAGGTGATGAGGTTGAACCCCGGTCACTACGTTTCCCTCATCATTCCATTGCCGGTTTCCGAACAAAATCACAAACAAGTAGCAGAGAATCACGAGGAGGAGCACGGCAAAGCGGTGCGTTTCACTCGCATTAAGCTTCTCCGGCCGAACGAAACTCTCGCTCTTGGCCATGCCTACCGGCTGGTCACCACTCAAGAGGTTATGAAGGTTTTGAAGGCAAAAAAATATGCGAAAACGAAGAAACAGCAGCTCGAAGTGCTCGAGAAGTTGCAAACGGAGCAAGGGAAGGAGAATTCAGGTTGTTCGGGCTGCAAAGAGGAAGCAGCGAAATCTGATGAGAAGGAAAATATCAACCATGCTTCAAAACGCGAAAGGCACCGACCAGGGACATCATCAGCGAATGCTGCTGCTGCGGCTGCTGCTGCGCTGAGGTCGAAGTCTTGGCGCCCCTCGTTGCAGAGCATCTGTGAGGGCGCAACTGATCAGCGGTTTCCAAACATCGAAAGTGAACACAATGAAAGTAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

208

Amino Acids

23.08

Weight (kDa)

9.14

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 188 1.6e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 468, 537
AccI GTMKAC 1 cut(s) 616
AccII CGCG 1 cut(s) 462
AciI CCGC 3 cut(s) 206, 506, 580
AcoI YGGCCR 2 cut(s) 236, 259
AcsI RAATTY 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 538
AgsI TTSAA 3 cut(s) 109, 307, 456
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 3 cut(s) 32, 229, 343
AluI AGCT 3 cut(s) 32, 229, 343
Alw21I GWGCWC 2 cut(s) 198, 354
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 3 cut(s) 77, 236, 259
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 3 cut(s) 520, 540, 569
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 49, 109, 271
AvaI CYCGRG 1 cut(s) 353
BalI TGGCCA 1 cut(s) 261
BanI GGYRCC 2 cut(s) 468, 537
BauI CACGAG 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 198, 354
BbvCI CCTCAGC 1 cut(s) 521
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 477
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 114, 477
BmeT110I CYCGRG 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 470, 539
BmrFI CCNGG 2 cut(s) 114, 477
BmsI GCATC 3 cut(s) 13, 55, 564
Bpu10I CCTNAGC 1 cut(s) 521
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 2 cut(s) 112, 476
Bse118I RCCGGY 2 cut(s) 145, 270
Bse3DI GCAATG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 112, 476
BseGI GGATG 2 cut(s) 46, 75
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 512
BseRI GAGGAG 2 cut(s) 203, 206
BseX3I CGGCCG 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 462
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 3 cut(s) 79, 238, 261
BshNI GGYRCC 2 cut(s) 468, 537
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 2 cut(s) 198, 354
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 4 cut(s) 114, 146, 235, 271
BslFI GGGAC 1 cut(s) 493
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 3 cut(s) 79, 238, 261
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 2 cut(s) 198, 354
Bsp143I GATC 1 cut(s) 574
BspACI CCGC 3 cut(s) 206, 506, 580
BspANI GGCC 3 cut(s) 79, 238, 261
BspCNI CTCAG 1 cut(s) 513
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 2 cut(s) 470, 539
BspT107I GGYRCC 2 cut(s) 468, 537
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 2 cut(s) 145, 270
BssAI RCCGGY 2 cut(s) 145, 270
BssECI CCNNGG 2 cut(s) 112, 476
BssMI GATC 1 cut(s) 574
BssNI GRCGYC 1 cut(s) 538
BssSI CACGAG 1 cut(s) 185
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 538
BstDEI CTNAG 1 cut(s) 521
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 2 cut(s) 46, 75
BstFNI CGCG 1 cut(s) 462
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 3 cut(s) 520, 540, 569
BstKTI GATC 1 cut(s) 577
BstMBI GATC 1 cut(s) 574
BstMCI CGRYCG 1 cut(s) 239
BstNI CCWGG 1 cut(s) 477
BstPI GGTNACC 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 112, 475
BstUI CGCG 1 cut(s) 462
BstZI CGGCCG 1 cut(s) 236
BsuRI GGCC 3 cut(s) 79, 238, 261
BtsCI GGATG 2 cut(s) 46, 75
CfoI GCGC 3 cut(s) 520, 540, 569
Cfr10I RCCGGY 2 cut(s) 145, 270
CviAII CATG 2 cut(s) 263, 449
DdeI CTNAG 1 cut(s) 521
DinI GGCGCC 1 cut(s) 539
DpnI GATC 1 cut(s) 576
DpnII GATC 1 cut(s) 574
EaeI YGGCCR 2 cut(s) 236, 259
EagI CGGCCG 1 cut(s) 236
EclXI CGGCCG 1 cut(s) 236
Eco52I CGGCCG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 475
EgeI GGCGCC 1 cut(s) 539
EheI GGCGCC 1 cut(s) 539
FaeI CATG 2 cut(s) 266, 452
FaiI YATR 5 cut(s) 83, 264, 296, 321, 450
FaqI GGGAC 1 cut(s) 493
FatI CATG 2 cut(s) 262, 448
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 616
FokI GGATG 2 cut(s) 33, 82
GlaI GCGC 3 cut(s) 519, 539, 568
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 3 cut(s) 79, 238, 261
HapII CCGG 4 cut(s) 114, 146, 235, 271
HhaI GCGC 3 cut(s) 520, 540, 569
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 2 cut(s) 266, 452
Hin6I GCGC 3 cut(s) 518, 538, 567
HinP1I GCGC 3 cut(s) 518, 538, 567
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 181
HpaII CCGG 4 cut(s) 114, 146, 235, 271
HphI GGTGA 3 cut(s) 49, 109, 271
Hpy166II GTNNAC 2 cut(s) 602, 617
Hpy188I TCNGA 3 cut(s) 51, 154, 427
Hpy188III TCNNGA 3 cut(s) 185, 287, 355
Hpy8I GTNNAC 2 cut(s) 602, 617
HpyAV CCTTC 4 cut(s) 292, 301, 373, 427
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 4 cut(s) 26, 364, 405, 550
HpyF3I CTNAG 1 cut(s) 521
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 2 cut(s) 266, 452
HspAI GCGC 3 cut(s) 518, 538, 567
KasI GGCGCC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 1 cut(s) 574
LmnI GCTCC 2 cut(s) 193, 370
LpnPI CCDG 9 cut(s) 20, 127, 159, 248, 260, 284, 375, 462, 489
LweI GCATC 3 cut(s) 13, 55, 564
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 116, 277
MalI GATC 1 cut(s) 576
MboI GATC 1 cut(s) 574
MboII GAAGA 2 cut(s) 70, 343
MhlI GDGCHC 2 cut(s) 198, 354
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 1 cut(s) 385
MluNI TGGCCA 1 cut(s) 261
Mly113I GGCGCC 1 cut(s) 538
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 580
MspI CCGG 4 cut(s) 114, 146, 235, 271
MspR9I CCNGG 2 cut(s) 114, 477
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 477
MvnI CGCG 1 cut(s) 462
NarI GGCGCC 1 cut(s) 538
NciI CCSGG 1 cut(s) 114
NdeII GATC 1 cut(s) 574
NlaIII CATG 2 cut(s) 266, 452
NlaIV GGNNCC 2 cut(s) 470, 539
NmuCI GTSAC 2 cut(s) 116, 277
PaeR7I CTCGAG 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 181
PluTI GGCGCC 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 475
PspEI GGTNACC 1 cut(s) 277
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 2 cut(s) 470, 539
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 225
Sau3AI GATC 1 cut(s) 574
ScrFI CCNGG 2 cut(s) 114, 477
SduI GDGCHC 2 cut(s) 198, 354
SfaNI GCATC 3 cut(s) 13, 55, 564
SfoI GGCGCC 1 cut(s) 539
Sfr274I CTCGAG 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 285, 353
SmoI CTYRAG 2 cut(s) 285, 353
Sse9I AATT 1 cut(s) 385
SsiI CCGC 3 cut(s) 206, 506, 580
SspDI GGCGCC 1 cut(s) 537
StyD4I CCNGG 2 cut(s) 112, 475
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 126
TaqI TCGA 4 cut(s) 345, 354, 527, 594
TasI AATT 1 cut(s) 385
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseFI GTSAC 2 cut(s) 116, 277
Tsp45I GTSAC 2 cut(s) 116, 277
TspDTI ATGAA 2 cut(s) 311, 624
TspGWI ACGGA 1 cut(s) 383
XapI RAATTY 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.