AT1G11400

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008395
Physical Location & Seq
Forward (+)
3837694 .. 3841218
3525 bp
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UTR
Exon/CDS
Intron
AT1G11400.4

Sequence Viewer

Length: 615 bp
ATGGGAAGCAGAAGCGGAGAACAAGGAAAGCGAATGGCGGAACTGAGCAAAAATCTTAAAGAAGGTGAGAGGATTCTGGAGCCCACACGTAGACCTGATGGTACTCTCCGGAAACCCATTCGGATTCGACCTGGATATACTCCAGAAGATGAAGTTGTCAAATATCAGTCCAAAGGTTCTCTGATGAAGAAGGAGATGGCCTCACAAGGGCCTCCAGGTTATGAACCTGATCCAGCTCCTAAACCAAAGACTAAAGCTGCTAAGAGGAACGAACGTAAAAAAGAGAAGCGATTACAGGCTACTGCAGAAAAAGCTAACAGCTCTGAGGATGGATCAGCTAGTAATGGATCCCAATCGGTGAATGTATTGGCTTCTGAAATGGAGGCTTTGGACGTTTCGTCAAATAATGATGTATGTGGGGGAGCACCTAATCCAGGAACCACAGGAGAAGACGTAGAGAAAAGAATCCGCGCACTTAAGAAGAAAATCCGACTCACAGAAGCTCAACAGCAGAAGACTGCTTCCCGAGACCTAAACCCAGAGCAGTTAGAGAAATTCTCTAAGTTGGAAGAATGGAGGCAAGAACTCAAGGCTCTGGAGGATAAGGCGGCTTAA

Protein Analysis

204

Amino Acids

22.59

Weight (kDa)

9.46

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mago-bind PF09282 22 - 48 2.3e-13 Mago binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 91
AccII CGCG 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 4 cut(s) 15, 38, 469, 608
AclWI GGATC 4 cut(s) 224, 340, 342, 355
AcsI RAATTY 1 cut(s) 554
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 2 cut(s) 207, 434
AflII CTTAAG 1 cut(s) 476
AflIII ACRYGT 1 cut(s) 86
AhdI GACNNNNNGTC 1 cut(s) 397
AjnI CCWGG 3 cut(s) 130, 214, 433
AluBI AGCT 6 cut(s) 236, 257, 314, 321, 338, 503
AluI AGCT 6 cut(s) 236, 257, 314, 321, 338, 503
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 4 cut(s) 224, 340, 342, 355
Ama87I CYCGRG 1 cut(s) 525
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 2 cut(s) 198, 209
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 554
AspLEI GCGC 1 cut(s) 473
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 77, 370
AvaI CYCGRG 1 cut(s) 525
BamHI GGATCC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 2 cut(s) 456, 521
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 244
BccI CCATC 3 cut(s) 92, 190, 323
BciT130I CCWGG 3 cut(s) 132, 216, 435
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 303
BfrI CTTAAG 1 cut(s) 476
BisI GCNGC 2 cut(s) 258, 609
BlsI GCNGC 2 cut(s) 259, 610
Bme1390I CCNGG 3 cut(s) 132, 216, 435
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 3 cut(s) 81, 349, 439
BmrFI CCNGG 3 cut(s) 132, 216, 435
BpiI GAAGAC 2 cut(s) 456, 521
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 542, 574
BpmI CTGGAG 3 cut(s) 98, 126, 198
BpuEI CTTGAG 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 89
BsaBI GATNNNNATC 1 cut(s) 352
BsaI GGTCTC 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 434
Bse8I GATNNNNATC 1 cut(s) 352
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 3 cut(s) 132, 216, 435
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 352
BseLI CCNNNNNNNGG 2 cut(s) 207, 434
BseMII CTCAG 2 cut(s) 35, 315
BseXI GCAGC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 200, 211
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 525
BsiSI CCGG 1 cut(s) 109
BslI CCNNNNNNNGG 2 cut(s) 207, 434
BsmAI GTCTC 1 cut(s) 522
BsnI GGCC 2 cut(s) 200, 211
Bso31I GGTCTC 1 cut(s) 522
BsoBI CYCGRG 1 cut(s) 525
Bsp1286I GDGCHC 2 cut(s) 84, 427
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 3 cut(s) 229, 332, 347
BspACI CCGC 4 cut(s) 15, 38, 469, 608
BspANI GGCC 2 cut(s) 200, 211
BspCNI CTCAG 2 cut(s) 36, 316
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 81, 349, 439
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 4 cut(s) 224, 340, 342, 355
BspTI CTTAAG 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 522
BssMI GATC 3 cut(s) 229, 332, 347
Bst2UI CCWGG 3 cut(s) 132, 216, 435
BstAFI CTTAAG 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 89
BstDEI CTNAG 4 cut(s) 44, 261, 324, 561
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 471
BstHHI GCGC 1 cut(s) 473
BstKTI GATC 3 cut(s) 232, 335, 350
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 3 cut(s) 229, 332, 347
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 3 cut(s) 132, 216, 435
BstSCI CCNGG 3 cut(s) 130, 214, 433
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 2 cut(s) 456, 521
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
BsuRI GGCC 2 cut(s) 200, 211
BtsCI GGATG 1 cut(s) 334
CfoI GCGC 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 102
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 4 cut(s) 44, 261, 324, 561
DpnI GATC 3 cut(s) 231, 334, 349
DpnII GATC 3 cut(s) 229, 332, 347
DriI GACNNNNNGTC 1 cut(s) 397
Eam1105I GACNNNNNGTC 1 cut(s) 397
EciI GGCGGA 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 84
Eco31I GGTCTC 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 130, 214, 433
EcoT38I GRGCYC 1 cut(s) 84
FaiI YATR 3 cut(s) 138, 222, 415
FblI GTMKAC 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 258, 609
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 258, 609
FspBI CTAG 1 cut(s) 339
GlaI GCGC 1 cut(s) 472
GluI GCNGC 2 cut(s) 258, 609
GsuI CTGGAG 3 cut(s) 98, 126, 198
HaeIII GGCC 2 cut(s) 200, 211
HapII CCGG 1 cut(s) 109
HhaI GCGC 1 cut(s) 473
Hin6I GCGC 1 cut(s) 471
HinP1I GCGC 1 cut(s) 471
HinfI GANTC 4 cut(s) 73, 124, 465, 492
HpaII CCGG 1 cut(s) 109
HphI GGTGA 2 cut(s) 77, 370
Hpy166II GTNNAC 1 cut(s) 92
Hpy188I TCNGA 5 cut(s) 123, 183, 325, 376, 491
Hpy188III TCNNGA 5 cut(s) 77, 109, 143, 525, 596
Hpy8I GTNNAC 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 56, 184
HpyCH4IV ACGT 4 cut(s) 88, 274, 393, 453
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 4 cut(s) 44, 261, 324, 561
HpySE526I ACGT 4 cut(s) 88, 274, 393, 453
HspAI GCGC 1 cut(s) 471
Kpn2I TCCGGA 1 cut(s) 108
Kzo9I GATC 3 cut(s) 229, 332, 347
LmnI GCTCC 3 cut(s) 79, 241, 422
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 1 cut(s) 339
MaeII ACGT 4 cut(s) 88, 274, 393, 453
MalI GATC 3 cut(s) 231, 334, 349
MboI GATC 3 cut(s) 229, 332, 347
MboII GAAGA 6 cut(s) 158, 199, 461, 493, 526, 581
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 2 cut(s) 84, 427
MluCI AATT 1 cut(s) 554
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 2 cut(s) 514, 546
MnlI CCTC 8 cut(s) 63, 211, 222, 258, 319, 376, 570, 592
MroI TCCGGA 1 cut(s) 108
MseI TTAA 3 cut(s) 57, 477, 613
MspCI CTTAAG 1 cut(s) 476
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 3 cut(s) 132, 216, 435
MvaI CCWGG 3 cut(s) 132, 216, 435
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 1 cut(s) 311
NdeII GATC 3 cut(s) 229, 332, 347
NlaIV GGNNCC 3 cut(s) 81, 349, 439
PfeI GAWTC 3 cut(s) 73, 124, 465
PfoI TCCNGGA 1 cut(s) 433
PkrI GCNGC 2 cut(s) 259, 610
PleI GAGTC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 486
Ppu21I YACGTR 1 cut(s) 89
Psp6I CCWGG 3 cut(s) 130, 214, 433
PspGI CCWGG 3 cut(s) 130, 214, 433
PspN4I GGNNCC 3 cut(s) 81, 349, 439
PspPI GGNCC 1 cut(s) 209
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SaqAI TTAA 3 cut(s) 57, 477, 613
SatI GCNGC 2 cut(s) 258, 609
Sau3AI GATC 3 cut(s) 229, 332, 347
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 3 cut(s) 132, 216, 435
SduI GDGCHC 2 cut(s) 84, 427
SfcI CTRYAG 1 cut(s) 303
SmlI CTYRAG 2 cut(s) 476, 587
SmoI CTYRAG 2 cut(s) 476, 587
Sse9I AATT 1 cut(s) 554
SsiI CCGC 4 cut(s) 15, 38, 469, 608
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 3 cut(s) 130, 214, 433
TaiI ACGT 4 cut(s) 91, 277, 396, 456
TaqI TCGA 1 cut(s) 127
TasI AATT 1 cut(s) 554
TauI GCSGC 1 cut(s) 611
TfiI GAWTC 3 cut(s) 73, 124, 465
Tru1I TTAA 3 cut(s) 57, 477, 613
Tru9I TTAA 3 cut(s) 57, 477, 613
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 3 cut(s) 165, 200, 237
Vha464I CTTAAG 1 cut(s) 476
XagI CCTNNNNNAGG 1 cut(s) 432
XapI RAATTY 1 cut(s) 554
XmiI GTMKAC 1 cut(s) 91
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.