MD08G1225100.v1.1

Partner of Y14 and

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008395
Physical Location & Seq
Forward (+)
29024333 .. 29028957
4625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1225100.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCAAGCAGCAACAATGGAAGCGGAGGAGGAAAAGATCAGCCGCTGGAGAAATTGGCGGAGCTTAGCAAAACCCTAAAAGAAGGGGAGAGGCTTCTGGCGCCAACCCGACGACCCGACGGTACGCTCCGTAAACCCATCCGCATTCGGGCTGGATATGTTCCCCAAGAGGAAGTCGCCATTTACCAATCCAAAGGTGCTTTGTGGAAAAAGGAGATGGCCTCAGTGGCTGGACCTCCGGGTTATGATGATACTTCCGTTGATACAGCGAAACCCAAGACTAAGTCTGTAAAGAGGAACGAAAGAAAAAAGGAGAAGCGGATACAGGCTGCTCTTGAAAAGGAGAAAACCTCAGAAAGTGGGGAAACTAAGGAAGAAGAAGTCGAAAATTTAGGCCATGTATCAGAAGCTGTCGAGTCATTGGCATCTCAGATGAACGAGCTAGGTGTTTCTTCTAGTCCTCCTTTAGTTACCCCTCCCTCTGATTCAACAGAGGATTCGAATCCAGCTGGTCCAGCTCAAGATATAGATAAAAGAATTCGAGCTCTTAAAAAGAAGATTCGACTTGCAGAAGCTCAACAGCAGAAAACTAATCAGCAAGATATCAAGTCAGAGCAGTTGGAGAAGTTGACGAAACTGGAGGGTTGGCGTAAGGAGCTTAAGCTTTTGGAGGATAAAAAGAAGGCTGAGCCGGCCGCACTGTGA

Protein Analysis

235

Amino Acids

25.78

Weight (kDa)

9.12

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mago-bind PF09282 29 - 55 1.2e-12 Mago binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AciI CCGC 6 cut(s) 24, 44, 59, 142, 319, 696
AcoI YGGCCR 1 cut(s) 693
AcsI RAATTY 2 cut(s) 388, 537
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 1 cut(s) 124
AfiI CCNNNNNNNGG 1 cut(s) 148
AflII CTTAAG 1 cut(s) 659
AgsI TTSAA 2 cut(s) 338, 489
AluBI AGCT 9 cut(s) 64, 410, 442, 509, 518, 545, 575, 658, 664
AluI AGCT 9 cut(s) 64, 410, 442, 509, 518, 545, 575, 658, 664
Alw21I GWGCWC 1 cut(s) 547
AlwNI CAGNNNCTG 3 cut(s) 46, 230, 410
AoxI GGCC 3 cut(s) 219, 394, 693
ApeKI GCWGC 2 cut(s) 9, 329
ApoI RAATTY 2 cut(s) 388, 537
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 233, 512
AsuC2I CCSGG 1 cut(s) 240
AsuII TTCGAA 1 cut(s) 500
AvaII GGWCC 2 cut(s) 233, 512
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BanI GGYRCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 2 cut(s) 21, 316
BccI CCATC 2 cut(s) 146, 211
BciVI GTATCC 1 cut(s) 315
BcnI CCSGG 1 cut(s) 240
BfaI CTAG 2 cut(s) 443, 456
BfoI RGCGCY 1 cut(s) 104
BfrI CTTAAG 1 cut(s) 659
BfuI GTATCC 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 4 cut(s) 10, 44, 330, 696
BlpI GCTNAGC 2 cut(s) 65, 687
BlsI GCNGC 4 cut(s) 11, 45, 331, 697
Bme1390I CCNGG 1 cut(s) 240
Bme18I GGWCC 2 cut(s) 233, 512
BmgT120I GGNCC 2 cut(s) 233, 512
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 434
BplI GAGNNNNNCTC 4 cut(s) 206, 238, 335, 367
BpmI CTGGAG 2 cut(s) 68, 659
Bpu1102I GCTNAGC 2 cut(s) 65, 687
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 240
BsaBI GATNNNNATC 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 101
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 501
BseGI GGATG 1 cut(s) 138
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 4 cut(s) 237, 366, 443, 678
BseNI ACTGG 1 cut(s) 642
BseRI GAGGAG 1 cut(s) 42
BseX3I CGGCCG 1 cut(s) 693
BseXI GCAGC 2 cut(s) 21, 316
Bsh1285I CGRYCG 1 cut(s) 696
BshFI GGCC 3 cut(s) 221, 396, 695
BshNI GGYRCC 1 cut(s) 100
BsiEI CGRYCG 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 2 cut(s) 239, 692
BslI CCNNNNNNNGG 1 cut(s) 148
BsmI GAATGC 1 cut(s) 144
BsnI GGCC 3 cut(s) 221, 396, 695
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 1 cut(s) 37
Bsp1720I GCTNAGC 2 cut(s) 65, 687
BspACI CCGC 6 cut(s) 24, 44, 59, 142, 319, 696
BspANI GGCC 3 cut(s) 221, 396, 695
BspCNI CTCAG 4 cut(s) 236, 365, 442, 679
BspLI GGNNCC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 100
BspTI CTTAAG 1 cut(s) 659
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 1 cut(s) 642
BssAI RCCGGY 1 cut(s) 691
BssMI GATC 1 cut(s) 37
BssNI GRCGYC 1 cut(s) 101
Bst4CI ACNGT 2 cut(s) 122, 702
BstACI GRCGYC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 659
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 7, 693
BstDEI CTNAG 7 cut(s) 65, 223, 282, 352, 369, 429, 687
BstF5I GGATG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 1 cut(s) 40
BstMBI GATC 1 cut(s) 37
BstMCI CGRYCG 1 cut(s) 696
BstMWI GCNNNNNNNGC 5 cut(s) 100, 227, 515, 655, 692
BstSCI CCNGG 1 cut(s) 238
BstV1I GCAGC 2 cut(s) 21, 316
BstZI CGGCCG 1 cut(s) 693
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 3 cut(s) 221, 396, 695
BtsCI GGATG 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 231, 698
Cac8I GCNNGC 2 cut(s) 7, 693
CaiI CAGNNNCTG 3 cut(s) 46, 230, 410
CfoI GCGC 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 2 cut(s) 233, 512
Csp6I GTAC 1 cut(s) 123
CviAII CATG 1 cut(s) 398
CviQI GTAC 1 cut(s) 123
DdeI CTNAG 7 cut(s) 65, 223, 282, 352, 369, 429, 687
DinI GGCGCC 1 cut(s) 102
DpnI GATC 1 cut(s) 39
DpnII GATC 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 693
EagI CGGCCG 1 cut(s) 693
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 545
EclXI CGGCCG 1 cut(s) 693
Eco24I GRGCYC 1 cut(s) 547
Eco32I GATATC 1 cut(s) 604
Eco47I GGWCC 2 cut(s) 233, 512
Eco52I CGGCCG 1 cut(s) 693
Eco53kI GAGCTC 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 537
EcoRV GATATC 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 547
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
FaeI CATG 1 cut(s) 401
FaiI YATR 4 cut(s) 159, 246, 399, 527
FatI CATG 1 cut(s) 397
Fnu4HI GCNGC 4 cut(s) 10, 44, 330, 696
FokI GGATG 1 cut(s) 125
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 4 cut(s) 10, 44, 330, 696
FspBI CTAG 2 cut(s) 443, 456
GlaI GCGC 1 cut(s) 102
GluI GCNGC 4 cut(s) 10, 44, 330, 696
GsuI CTGGAG 2 cut(s) 68, 659
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 3 cut(s) 221, 396, 695
HapII CCGG 2 cut(s) 239, 692
HhaI GCGC 1 cut(s) 103
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 1 cut(s) 401
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HincII GTYRAC 1 cut(s) 630
HindII GTYRAC 1 cut(s) 630
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 5 cut(s) 416, 485, 497, 502, 559
HpaII CCGG 2 cut(s) 239, 692
Hpy166II GTNNAC 2 cut(s) 134, 630
Hpy188I TCNGA 5 cut(s) 355, 406, 432, 484, 613
Hpy188III TCNNGA 2 cut(s) 335, 521
Hpy8I GTNNAC 2 cut(s) 134, 630
Hpy99I CGWCG 2 cut(s) 114, 122
HpyAV CCTTC 2 cut(s) 77, 676
HpyCH4III ACNGT 2 cut(s) 122, 702
HpyCH4V TGCA 1 cut(s) 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 227, 515, 655, 692
HpyF3I CTNAG 7 cut(s) 65, 223, 282, 352, 369, 429, 687
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 1 cut(s) 401
HspAI GCGC 1 cut(s) 101
KasI GGCGCC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 691
KroNI GCCGGC 1 cut(s) 693
Kzo9I GATC 1 cut(s) 37
LmnI GCTCC 3 cut(s) 61, 132, 655
Lsp1109I GCAGC 2 cut(s) 21, 316
LweI GCATC 1 cut(s) 434
MaeI CTAG 2 cut(s) 443, 456
MaeIII GTNAC 1 cut(s) 469
MalI GATC 1 cut(s) 39
MboI GATC 1 cut(s) 37
MboII GAAGA 4 cut(s) 386, 389, 444, 568
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 3 cut(s) 53, 388, 537
Mly113I GGCGCC 1 cut(s) 101
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 600
MroNI GCCGGC 1 cut(s) 691
MseI TTAA 2 cut(s) 549, 660
MspA1I CMGCKG 2 cut(s) 46, 509
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 2 cut(s) 239, 692
MspR9I CCNGG 1 cut(s) 240
Mva1269I GAATGC 1 cut(s) 144
MwoI GCNNNNNNNGC 5 cut(s) 100, 227, 515, 655, 692
NaeI GCCGGC 1 cut(s) 693
NarI GGCGCC 1 cut(s) 101
NciI CCSGG 1 cut(s) 240
NdeII GATC 1 cut(s) 37
NgoMIV GCCGGC 1 cut(s) 691
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 1 cut(s) 102
NspV TTCGAA 1 cut(s) 500
PctI GAATGC 1 cut(s) 144
PdiI GCCGGC 1 cut(s) 693
PfeI GAWTC 4 cut(s) 485, 497, 502, 559
PflFI GACNNNGTC 1 cut(s) 283
PkrI GCNGC 4 cut(s) 11, 45, 331, 697
PleI GAGTC 1 cut(s) 424
PluTI GGCGCC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 547
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 233, 512
PstNI CAGNNNCTG 3 cut(s) 46, 230, 410
PsyI GACNNNGTC 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 509
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 2 cut(s) 549, 660
SatI GCNGC 4 cut(s) 10, 44, 330, 696
Sau3AI GATC 1 cut(s) 37
Sau96I GGNCC 2 cut(s) 233, 512
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 1 cut(s) 547
SfaNI GCATC 1 cut(s) 434
SfoI GGCGCC 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 2 cut(s) 233, 512
SmlI CTYRAG 2 cut(s) 519, 659
SmoI CTYRAG 2 cut(s) 519, 659
Sse9I AATT 3 cut(s) 53, 388, 537
SsiI CCGC 6 cut(s) 24, 44, 59, 142, 319, 696
SspDI GGCGCC 1 cut(s) 100
SspMI CTAG 2 cut(s) 443, 456
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 122, 702
TaqI TCGA 5 cut(s) 384, 414, 500, 541, 562
TasI AATT 3 cut(s) 53, 388, 537
TauI GCSGC 2 cut(s) 46, 698
TfiI GAWTC 4 cut(s) 485, 497, 502, 559
Tru1I TTAA 2 cut(s) 549, 660
Tru9I TTAA 2 cut(s) 549, 660
TscAI CASTG 2 cut(s) 231, 705
TseI GCWGC 2 cut(s) 9, 329
TspDTI ATGAA 1 cut(s) 449
TspGWI ACGGA 2 cut(s) 119, 247
TspRI CASTG 2 cut(s) 231, 705
Tth111I GACNNNGTC 1 cut(s) 283
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 233, 512
XapI RAATTY 2 cut(s) 388, 537
XspI CTAG 2 cut(s) 443, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.