FvH4_5g30080

Partner of Y14 and

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008395
Physical Location & Seq
Reverse (-)
21008887 .. 21012189
3303 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30080.t1

Sequence Viewer

Length: 705 bp
ATGGCTCATCGCGGAGGAGGAGGAGATCAGGAGCTGAAACTGACGGAGGAGATCGGCAAATCCCTAAAAGAAGGGGAGAGGATTCTAGCGCCAACCCGGAGACCCGACGGCACGCTCCGCAAACCCATCCGCATTCGGGCCGGGTATGTGCCCCAGGAAGAAGTCGCCCTCTACCAATCCAAAGGTGCCTTGTGGAAAAAGGAGATGTCCTCACAGGCAGGGCCACCGGGTTTTGATGATACTACTCCCGTCGTGGCCGATGCGAAACCCAAGACCAAGTCTGTAAAAAGGAATGAAAGAAAGAAGGAGAAGCGGCTGCAGGCTGCTCTTGAGAAGGAGAAAACCTCAGAAGAAGCGGAAACTGGGGATACTAAGGAAGAAGAGCCACCTGCTCAAGGTGTAGGTCATGCATCAACATCAGTCCAGTCATTGACATCTCAGATGAACGAGCTTGGTGTTTCTTCTAATTCTGCTTTAGTTACCCCTTCCTCTGACTCAATAGAGGATTCAAATCTAGGAGCTCCAGTTCAAGATATCGATAAAAAAATCCGAGCACTTAAAAAAAAGATTCGACTTGCAGAAGCGCAGCAGCAGAAAACTCAGCAGAAAGATAGTAAGCCAGAGCAGTTGGAAAAGTTAACGAAACTGGAAGGATGGCGTCAGGAGTTAAAGCTTTTGGAAGATAAAAAGAAAGCTGAATCATGA

Protein Analysis

235

Amino Acids

25.83

Weight (kDa)

9.14

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mago-bind PF09282 25 - 51 6e-13 Mago binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 397
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 185
AccII CGCG 1 cut(s) 12
AciI CCGC 5 cut(s) 12, 118, 130, 313, 356
AcoI YGGCCR 1 cut(s) 255
AcyI GRCGYC 1 cut(s) 658
AfiI CCNNNNNNNGG 2 cut(s) 136, 395
AgsI TTSAA 2 cut(s) 510, 530
AjnI CCWGG 1 cut(s) 153
AloI GAACNNNNNNTCC 2 cut(s) 510, 542
AluBI AGCT 5 cut(s) 34, 451, 521, 673, 695
AluI AGCT 5 cut(s) 34, 451, 521, 673, 695
Alw21I GWGCWC 2 cut(s) 523, 556
Alw26I GTCTC 1 cut(s) 94
AlwNI CAGNNNCTG 1 cut(s) 34
AoxI GGCC 3 cut(s) 138, 221, 255
ApeKI GCWGC 4 cut(s) 316, 323, 586, 589
AspLEI GCGC 2 cut(s) 91, 586
AspS9I GGNCC 2 cut(s) 138, 221
AsuC2I CCSGG 3 cut(s) 97, 142, 228
BaeGI GKGCMC 1 cut(s) 153
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 523, 556
BbvI GCAGC 4 cut(s) 303, 310, 598, 601
BccI CCATC 2 cut(s) 134, 648
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 155
BciVI GTATCC 1 cut(s) 361
BcnI CCSGG 3 cut(s) 97, 142, 228
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 2 cut(s) 86, 515
BfmI CTRYAG 1 cut(s) 317
BfoI RGCGCY 1 cut(s) 92
BfuAI ACCTGC 1 cut(s) 397
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 5 cut(s) 314, 317, 324, 587, 590
BlsI GCNGC 5 cut(s) 315, 318, 325, 588, 591
Bme1390I CCNGG 4 cut(s) 97, 142, 155, 228
BmgT120I GGNCC 2 cut(s) 138, 221
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 4 cut(s) 97, 142, 155, 228
BmrI ACTGGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 250, 419
BmuI ACTGGG 1 cut(s) 372
BplI GAGNNNNNCTC 4 cut(s) 194, 226, 329, 361
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 350, 378
BpuMI CCSGG 3 cut(s) 97, 142, 228
Bsa29I ATCGAT 1 cut(s) 537
BsaBI GATNNNNATC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 658
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 395
Bse1I ACTGG 4 cut(s) 367, 424, 524, 651
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 2 cut(s) 126, 659
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 136, 395
BseMII CTCAG 3 cut(s) 360, 452, 614
BseNI ACTGG 4 cut(s) 367, 424, 524, 651
BseRI GAGGAG 4 cut(s) 30, 33, 36, 62
BseSI GKGCMC 1 cut(s) 153
BseXI GCAGC 4 cut(s) 303, 310, 598, 601
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 140, 223, 257
BshNI GGYRCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 523, 556
BsiSI CCGG 3 cut(s) 97, 141, 227
BslI CCNNNNNNNGG 2 cut(s) 136, 395
BsmAI GTCTC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 3 cut(s) 140, 223, 257
Bso31I GGTCTC 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 153, 523, 556
Bsp143I GATC 2 cut(s) 25, 51
BspACI CCGC 5 cut(s) 12, 118, 130, 313, 356
BspANI GGCC 3 cut(s) 140, 223, 257
BspCNI CTCAG 3 cut(s) 359, 451, 613
BspDI ATCGAT 1 cut(s) 537
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 1 cut(s) 701
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 1 cut(s) 397
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 94
BsrI ACTGG 4 cut(s) 367, 424, 524, 651
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 25, 51
BssNI GRCGYC 1 cut(s) 658
Bst2UI CCWGG 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 113, 321
BstDEI CTNAG 4 cut(s) 346, 372, 438, 600
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 2 cut(s) 126, 659
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 91, 586
BstKTI GATC 2 cut(s) 28, 54
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 2 cut(s) 25, 51
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 4 cut(s) 95, 140, 153, 226
BstSFI CTRYAG 1 cut(s) 317
BstSLI GKGCMC 1 cut(s) 153
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 4 cut(s) 303, 310, 598, 601
Bsu15I ATCGAT 1 cut(s) 537
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 3 cut(s) 140, 223, 257
BsuTUI ATCGAT 1 cut(s) 537
BtsCI GGATG 2 cut(s) 126, 659
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 113, 321
CaiI CAGNNNCTG 1 cut(s) 34
CciI TCATGA 1 cut(s) 701
CfoI GCGC 2 cut(s) 91, 586
Cfr13I GGNCC 2 cut(s) 138, 221
ClaI ATCGAT 1 cut(s) 537
CseI GACGC 1 cut(s) 647
CviAII CATG 2 cut(s) 407, 702
DdeI CTNAG 4 cut(s) 346, 372, 438, 600
DpnI GATC 2 cut(s) 27, 53
DpnII GATC 2 cut(s) 25, 51
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 521
EcoICRI GAGCTC 1 cut(s) 521
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 153
EcoRV GATATC 1 cut(s) 535
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 523
FaeI CATG 2 cut(s) 410, 705
FaiI YATR 3 cut(s) 147, 408, 703
FatI CATG 2 cut(s) 406, 701
Fnu4HI GCNGC 5 cut(s) 314, 317, 324, 587, 590
FokI GGATG 2 cut(s) 113, 666
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 5 cut(s) 314, 317, 324, 587, 590
FspBI CTAG 2 cut(s) 86, 515
GlaI GCGC 2 cut(s) 90, 585
GluI GCNGC 5 cut(s) 314, 317, 324, 587, 590
GsuI CTGGAG 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 3 cut(s) 140, 223, 257
HapII CCGG 3 cut(s) 97, 141, 227
HgaI GACGC 1 cut(s) 647
HhaI GCGC 2 cut(s) 91, 586
Hin1I GRCGYC 1 cut(s) 658
Hin1II CATG 2 cut(s) 410, 705
Hin6I GCGC 2 cut(s) 89, 584
HinP1I GCGC 2 cut(s) 89, 584
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 5 cut(s) 82, 494, 506, 568, 698
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 3 cut(s) 97, 141, 227
Hpy166II GTNNAC 1 cut(s) 639
Hpy188I TCNGA 4 cut(s) 349, 441, 493, 551
Hpy188III TCNNGA 5 cut(s) 29, 329, 530, 662, 702
Hpy8I GTNNAC 1 cut(s) 639
Hpy99I CGWCG 2 cut(s) 110, 254
HpyAV CCTTC 5 cut(s) 65, 298, 328, 495, 644
HpyCH4V TGCA 3 cut(s) 319, 410, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 4 cut(s) 346, 372, 438, 600
Hsp92I GRCGYC 1 cut(s) 658
Hsp92II CATG 2 cut(s) 410, 705
HspAI GCGC 2 cut(s) 89, 584
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 2 cut(s) 25, 51
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 4 cut(s) 31, 120, 518, 526
Lsp1109I GCAGC 4 cut(s) 303, 310, 598, 601
LweI GCATC 2 cut(s) 250, 419
MaeI CTAG 2 cut(s) 86, 515
MaeIII GTNAC 1 cut(s) 478
MalI GATC 2 cut(s) 27, 53
MboI GATC 2 cut(s) 25, 51
MboII GAAGA 6 cut(s) 170, 362, 389, 392, 453, 692
MhlI GDGCHC 3 cut(s) 153, 523, 556
MluCI AATT 1 cut(s) 466
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 609
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 3 cut(s) 558, 638, 668
MspI CCGG 3 cut(s) 97, 141, 227
MspR9I CCNGG 4 cut(s) 97, 142, 155, 228
Mva1269I GAATGC 1 cut(s) 132
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 117
NciI CCSGG 3 cut(s) 97, 142, 228
NdeII GATC 2 cut(s) 25, 51
NlaIII CATG 2 cut(s) 410, 705
NlaIV GGNNCC 1 cut(s) 187
NsiI ATGCAT 1 cut(s) 412
PagI TCATGA 1 cut(s) 701
PaqCI CACCTGC 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 1 cut(s) 132
PfeI GAWTC 4 cut(s) 82, 506, 568, 698
PflFI GACNNNGTC 1 cut(s) 277
PkrI GCNGC 5 cut(s) 315, 318, 325, 588, 591
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 138, 221
PstI CTGCAG 1 cut(s) 321
PstNI CAGNNNCTG 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 277
SacI GAGCTC 1 cut(s) 523
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 558, 638, 668
SatI GCNGC 5 cut(s) 314, 317, 324, 587, 590
Sau3AI GATC 2 cut(s) 25, 51
Sau96I GGNCC 2 cut(s) 138, 221
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 4 cut(s) 97, 142, 155, 228
SduI GDGCHC 3 cut(s) 153, 523, 556
SfaNI GCATC 2 cut(s) 250, 419
SfcI CTRYAG 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 329, 393
SmoI CTYRAG 2 cut(s) 329, 393
Sse9I AATT 1 cut(s) 466
SsiI CCGC 5 cut(s) 12, 118, 130, 313, 356
SspMI CTAG 2 cut(s) 86, 515
SstI GAGCTC 1 cut(s) 523
StyD4I CCNGG 4 cut(s) 95, 140, 153, 226
TaqI TCGA 2 cut(s) 537, 571
TasI AATT 1 cut(s) 466
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 4 cut(s) 82, 506, 568, 698
Tru1I TTAA 3 cut(s) 558, 638, 668
Tru9I TTAA 3 cut(s) 558, 638, 668
TseI GCWGC 4 cut(s) 316, 323, 586, 589
TspDTI ATGAA 2 cut(s) 309, 458
TspGWI ACGGA 1 cut(s) 59
Tth111I GACNNNGTC 1 cut(s) 277
XagI CCTNNNNNAGG 1 cut(s) 393
XspI CTAG 2 cut(s) 86, 515
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.