AT1G12120

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006281
Physical Location & Seq
Reverse (-)
4114674 .. 4117614
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12120.1

Sequence Viewer

Length: 1452 bp
ATGTATTTCTTCGGTTTGTCCAAAGAAGAAGTGTTGTCACAGAAACATGTTGTATCATATAAAGAGACTTCTAAGCAGAACCCAATCAATATGGTCACTCTGTTTGGCAGACAGGTGTATCAGGTAAAAAGTGAGAGCTCACCTAAGCTTCTGAAGAAAGATATCGATCTTAGGCTTCCTGCAGATCACTACGCTTATACTAACTTTCATGAGACACTTGGTTCTGAGAAATTTTACAGCAATGGTAGTTCCCGAGATACCGTAGAGATGAGATTTCATCCGGGGCACGAAGCTAATAATGTGAAGGAAGTATCAGCCACAAGTTGGACTGGCAAGAGAAATCCAAGAATTGTTATTGATCTTGAAGAACCTCCTCCCACATGGGTCTCCCATAGAGAGACTACAGAGCATGCTCAGGCTTCTGTTAGCTATGTTACTGTGAGAAATCCTGACACAAAACCGTCGTTCTTTGATCGGATCAGCTCAAACATTCTGATGGATGTAGACCAATCACTCGTTGAGGATGATAGTGATAAGACAACTAGTAAGGAGTCAAGTTTACTGGATCTTAATAGCACACCGGTTGATGAGTCTGTTTCCGAGCCTCGTTACTCTCTCTTACAAGATCTGAACTGTGCATACATCGAGGAGGAGACAGAAACGAGCTACGAGAAGAGTGGAATAGATGATGGTTCTACACCGCTTTGCTCTCCTCAATGCCAAAATGTCCATGAAAAAGACGGCACAGCATCTCCAGCTTCGGATACTTCTTGCTGCACCACTGAAAACAACTCGAGGATTGAGTCAAGAAGAAGTTCTTCTCCTCGAGCACTGCAGCCTTCTTGCCGTACCCGGCTTGAGTTTACCAACACAGAAGACTTACTGGAGGAGAATGGATGTTGTAACGAGGAGGATGATTCGTCTGAAGTGATTCAAATGGCAGCTGAGTCATTAGTCCACATATCGGAGATATCGTATCAAAACCAAGACTTGCAATCAAAGCTTGTGTTAAGAACCAACAGCTCATCTGAAGATCAAGATTTTCCAGATAAACCGGAGATGGGTAAAGCGAAACCGGGATGTTCCTACGATTCATATGAGCGACACACGTTAGGAATAAGCGAAACCAACACTGAAGAAGACTTCTGTGTGTCATCAATGGCTCTAGATGAACTTAATAACATCACAAGAGATAATAACAAAGAGATTGGACTCAAGCTGAGAAGAGGGAGAAGAATGAAGAACTTCCAAAAAGAGATACTCCCGAGCTTAACGTCGCTTTCTAGACATGAGATCCGGGAAGACATGAACATCTTGGAAGCAGTTTTGAGATCAAGAGAGTACAAGAAGATGCAGGGAAAGACGAAAGATGTCAAATTCAAAGCAAACCCAAGAAACAAACGTTCACCACCACAAAGATGCGTTGGTAAAAGAAGGCGAAAAACCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

483

Amino Acids

55.0

Weight (kDa)

5.46

Isoelectric Point (pI)

64.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 362 - 445 3e-09 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 324
AccI GTMKAC 1 cut(s) 504
AciI CCGC 1 cut(s) 701
AclI AACGTT 1 cut(s) 1402
AclWI GGATC 3 cut(s) 485, 573, 1288
AcsI RAATTY 2 cut(s) 230, 1376
AcuI CTGAAG 4 cut(s) 173, 945, 1050, 1155
AfaI GTAC 2 cut(s) 850, 1343
AfiI CCNNNNNNNGG 3 cut(s) 324, 964, 1061
AflIII ACRYGT 2 cut(s) 46, 1107
AgeI ACCGGT 1 cut(s) 580
AgsI TTSAA 3 cut(s) 365, 935, 1381
AhlI ACTAGT 1 cut(s) 542
Alw21I GWGCWC 2 cut(s) 140, 832
Alw26I GTCTC 5 cut(s) 59, 206, 391, 392, 647
AlwI GGATC 3 cut(s) 485, 573, 1288
Ama87I CYCGRG 4 cut(s) 252, 793, 825, 1264
ApeKI GCWGC 3 cut(s) 774, 835, 941
ApoI RAATTY 2 cut(s) 230, 1376
AsiGI ACCGGT 1 cut(s) 580
Asp700I GAANNNNTTC 4 cut(s) 814, 817, 930, 1244
AsuC2I CCSGG 4 cut(s) 282, 853, 1077, 1298
AsuHPI GGTGA 2 cut(s) 132, 1398
AvaI CYCGRG 4 cut(s) 252, 793, 825, 1264
BaeGI GKGCMC 1 cut(s) 288
BaeI ACNNNNGTAYC 2 cut(s) 101, 134
BanII GRGCYC 1 cut(s) 140
BbsI GAAGAC 3 cut(s) 882, 1146, 1308
Bbv12I GWGCWC 2 cut(s) 140, 832
BbvI GCAGC 3 cut(s) 761, 847, 953
BccI CCATC 3 cut(s) 490, 683, 1054
BceAI ACGGC 2 cut(s) 757, 831
BciVI GTATCC 1 cut(s) 757
BcnI CCSGG 4 cut(s) 282, 853, 1077, 1298
BcoDI GTCTC 5 cut(s) 59, 206, 391, 392, 647
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 3 cut(s) 543, 1166, 1284
BfmI CTRYAG 3 cut(s) 180, 402, 833
BfuI GTATCC 1 cut(s) 757
BglII AGATCT 1 cut(s) 625
BisI GCNGC 3 cut(s) 775, 836, 942
BlsI GCNGC 3 cut(s) 776, 837, 943
Bme1390I CCNGG 4 cut(s) 282, 853, 1077, 1298
BmeT110I CYCGRG 4 cut(s) 252, 793, 825, 1264
BmrFI CCNGG 4 cut(s) 282, 853, 1077, 1298
BmsI GCATC 3 cut(s) 758, 1341, 1409
BpiI GAAGAC 3 cut(s) 882, 1146, 1308
BpmI CTGGAG 2 cut(s) 738, 905
Bpu10I CCTNAGC 2 cut(s) 144, 414
BpuEI CTTGAG 2 cut(s) 878, 1199
BpuMI CCSGG 4 cut(s) 282, 853, 1077, 1298
Bsa29I ATCGAT 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 2 cut(s) 580, 1054
BsaXI ACNNNNNCTCC 4 cut(s) 736, 766, 959, 989
Bsc4I CCNNNNNNNGG 3 cut(s) 324, 964, 1061
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 3 cut(s) 334, 567, 888
Bse3DI GCAATG 1 cut(s) 247
Bse8I GATNNNNATC 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 6 cut(s) 277, 505, 529, 902, 919, 1085
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 3 cut(s) 324, 964, 1061
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 4 cut(s) 216, 428, 936, 1211
BseNI ACTGG 3 cut(s) 334, 567, 888
BseRI GAGGAG 7 cut(s) 363, 662, 665, 702, 813, 902, 923
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 3 cut(s) 761, 847, 953
BsgI GTGCAG 1 cut(s) 760
BshTI ACCGGT 1 cut(s) 580
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 140, 832
BsiHKCI CYCGRG 4 cut(s) 252, 793, 825, 1264
BsiSI CCGG 6 cut(s) 281, 581, 853, 1055, 1076, 1297
BslI CCNNNNNNNGG 3 cut(s) 324, 964, 1061
BsmAI GTCTC 5 cut(s) 59, 206, 391, 392, 647
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 4 cut(s) 252, 793, 825, 1264
Bsp1286I GDGCHC 3 cut(s) 140, 288, 832
BspACI CCGC 1 cut(s) 701
BspCNI CTCAG 4 cut(s) 217, 427, 937, 1212
BspDI ATCGAT 1 cut(s) 165
BspHI TCATGA 1 cut(s) 208
BspMAI CTGCAG 2 cut(s) 184, 837
BspPI GGATC 3 cut(s) 485, 573, 1288
BspTNI GGTCTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 3 cut(s) 334, 567, 888
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 281
Bst4CI ACNGT 4 cut(s) 262, 439, 462, 635
Bst6I CTCTTC 2 cut(s) 668, 1219
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 7 cut(s) 72, 144, 170, 225, 414, 945, 1220
BstF5I GGATG 6 cut(s) 277, 505, 529, 902, 919, 1085
BstMAI GTCTC 5 cut(s) 59, 206, 391, 392, 647
BstMWI GCNNNNNNNGC 2 cut(s) 755, 1000
BstNSI RCATGY 2 cut(s) 50, 413
BstSCI CCNGG 4 cut(s) 280, 851, 1075, 1296
BstSFI CTRYAG 3 cut(s) 180, 402, 833
BstSLI GKGCMC 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 761, 847, 953
BstV2I GAAGAC 3 cut(s) 882, 1146, 1308
BstX2I RGATCY 3 cut(s) 565, 625, 1293
BstYI RGATCY 3 cut(s) 565, 625, 1293
Bsu15I ATCGAT 1 cut(s) 165
BsuI GTATCC 1 cut(s) 757
BsuTUI ATCGAT 1 cut(s) 165
BtsCI GGATG 6 cut(s) 277, 505, 529, 902, 919, 1085
BtsI GCAGTG 1 cut(s) 830
BtsIMutI CAGTG 3 cut(s) 780, 830, 1131
Cac8I GCNNGC 1 cut(s) 411
CciI TCATGA 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 580
ClaI ATCGAT 1 cut(s) 165
Csp6I GTAC 2 cut(s) 849, 1342
CspAI ACCGGT 1 cut(s) 580
CviAII CATG 7 cut(s) 47, 209, 381, 410, 731, 1289, 1306
CviQI GTAC 2 cut(s) 849, 1342
DdeI CTNAG 7 cut(s) 72, 144, 170, 225, 414, 945, 1220
Eam1104I CTCTTC 2 cut(s) 668, 1219
EarI CTCTTC 2 cut(s) 668, 1219
Ecl136II GAGCTC 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 391
Eco32I GATATC 2 cut(s) 163, 972
Eco53kI GAGCTC 1 cut(s) 138
Eco57I CTGAAG 4 cut(s) 173, 945, 1050, 1155
Eco88I CYCGRG 4 cut(s) 252, 793, 825, 1264
EcoICRI GAGCTC 1 cut(s) 138
EcoRV GATATC 2 cut(s) 163, 972
EcoT38I GRGCYC 1 cut(s) 140
FaeI CATG 7 cut(s) 50, 212, 384, 413, 734, 1292, 1309
FalI AAGNNNNNCTT 2 cut(s) 802, 834
FatI CATG 7 cut(s) 46, 208, 380, 409, 730, 1288, 1305
FauNDI CATATG 1 cut(s) 1096
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 775, 836, 942
FokI GGATG 6 cut(s) 264, 512, 536, 909, 926, 1092
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 775, 836, 942
FspBI CTAG 3 cut(s) 543, 1166, 1284
GluI GCNGC 3 cut(s) 775, 836, 942
GsuI CTGGAG 2 cut(s) 738, 905
HapII CCGG 6 cut(s) 281, 581, 853, 1055, 1076, 1297
Hin1II CATG 7 cut(s) 50, 212, 384, 413, 734, 1292, 1309
HindIII AAGCTT 2 cut(s) 146, 1001
HinfI GANTC 8 cut(s) 551, 590, 803, 917, 931, 947, 1091, 1212
HpaII CCGG 6 cut(s) 281, 581, 853, 1055, 1076, 1297
HphI GGTGA 2 cut(s) 132, 1398
Hpy166II GTNNAC 5 cut(s) 505, 560, 864, 958, 1406
Hpy8I GTNNAC 5 cut(s) 505, 560, 864, 958, 1406
Hpy99I CGWCG 2 cut(s) 466, 1279
HpyAV CCTTC 3 cut(s) 298, 849, 1428
HpyCH4III ACNGT 4 cut(s) 262, 439, 462, 635
HpyCH4IV ACGT 3 cut(s) 1109, 1274, 1402
HpyCH4V TGCA 6 cut(s) 182, 638, 777, 835, 994, 1354
HpyF10VI GCNNNNNNNGC 2 cut(s) 755, 1000
HpyF3I CTNAG 7 cut(s) 72, 144, 170, 225, 414, 945, 1220
HpySE526I ACGT 3 cut(s) 1109, 1274, 1402
Hsp92II CATG 7 cut(s) 50, 212, 384, 413, 734, 1292, 1309
Lsp1109I GCAGC 3 cut(s) 761, 847, 953
LweI GCATC 3 cut(s) 758, 1341, 1409
MaeI CTAG 3 cut(s) 543, 1166, 1284
MaeII ACGT 3 cut(s) 1109, 1274, 1402
MaeIII GTNAC 5 cut(s) 36, 94, 433, 608, 902
MflI RGATCY 3 cut(s) 565, 625, 1293
MhlI GDGCHC 3 cut(s) 140, 288, 832
MluCI AATT 3 cut(s) 230, 348, 1376
MlyI GAGTC 5 cut(s) 560, 599, 812, 956, 1206
MmeI TCCRAC 1 cut(s) 305
MroXI GAANNNNTTC 4 cut(s) 814, 817, 930, 1244
MseI TTAA 4 cut(s) 570, 1010, 1176, 1271
MslI CAYNNNNRTG 2 cut(s) 494, 1417
MspA1I CMGCKG 1 cut(s) 944
MspI CCGG 6 cut(s) 281, 581, 853, 1055, 1076, 1297
MspR9I CCNGG 4 cut(s) 282, 853, 1077, 1298
MwoI GCNNNNNNNGC 2 cut(s) 755, 1000
NciI CCSGG 4 cut(s) 282, 853, 1077, 1298
NdeI CATATG 1 cut(s) 1096
NlaIII CATG 7 cut(s) 50, 212, 384, 413, 734, 1292, 1309
NmuCI GTSAC 2 cut(s) 36, 94
NspI RCATGY 2 cut(s) 50, 413
PaeI GCATGC 1 cut(s) 413
PaeR7I CTCGAG 2 cut(s) 793, 825
PagI TCATGA 1 cut(s) 208
PciI ACATGT 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 971
PdmI GAANNNNTTC 4 cut(s) 814, 817, 930, 1244
PfeI GAWTC 3 cut(s) 917, 931, 1091
PflMI CCANNNNNTGG 1 cut(s) 324
PfoI TCCNGGA 1 cut(s) 1296
PinAI ACCGGT 1 cut(s) 580
PkrI GCNGC 3 cut(s) 776, 837, 943
PleI GAGTC 5 cut(s) 559, 598, 811, 955, 1206
PpsI GAGTC 5 cut(s) 559, 598, 811, 955, 1206
PscI ACATGT 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 140
Psp1406I AACGTT 1 cut(s) 1402
PspXI VCTCGAGB 2 cut(s) 793, 825
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
PstI CTGCAG 2 cut(s) 184, 837
PsuI RGATCY 3 cut(s) 565, 625, 1293
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 2 cut(s) 850, 1343
RsaNI GTAC 2 cut(s) 849, 1342
RseI CAYNNNNRTG 2 cut(s) 494, 1417
SacI GAGCTC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 570, 1010, 1176, 1271
SatI GCNGC 3 cut(s) 775, 836, 942
SchI GAGTC 5 cut(s) 560, 599, 812, 956, 1206
ScrFI CCNGG 4 cut(s) 282, 853, 1077, 1298
SduI GDGCHC 3 cut(s) 140, 288, 832
SfaNI GCATC 3 cut(s) 758, 1341, 1409
SfcI CTRYAG 3 cut(s) 180, 402, 833
Sfr274I CTCGAG 2 cut(s) 793, 825
SlaI CTCGAG 2 cut(s) 793, 825
SmiMI CAYNNNNRTG 2 cut(s) 494, 1417
SmlI CTYRAG 4 cut(s) 793, 825, 857, 1214
SmoI CTYRAG 4 cut(s) 793, 825, 857, 1214
SpeI ACTAGT 1 cut(s) 542
SphI GCATGC 1 cut(s) 413
Sse9I AATT 3 cut(s) 230, 348, 1376
SsiI CCGC 1 cut(s) 701
SspMI CTAG 3 cut(s) 543, 1166, 1284
SstI GAGCTC 1 cut(s) 140
StyD4I CCNGG 4 cut(s) 280, 851, 1075, 1296
TaaI ACNGT 4 cut(s) 262, 439, 462, 635
TaiI ACGT 3 cut(s) 1112, 1277, 1405
TaqI TCGA 4 cut(s) 165, 645, 794, 826
TasI AATT 3 cut(s) 230, 348, 1376
TatI WGTACW 1 cut(s) 1341
TfiI GAWTC 3 cut(s) 917, 931, 1091
Tru1I TTAA 4 cut(s) 570, 1010, 1176, 1271
Tru9I TTAA 4 cut(s) 570, 1010, 1176, 1271
TscAI CASTG 3 cut(s) 787, 837, 1138
TseFI GTSAC 2 cut(s) 36, 94
TseI GCWGC 3 cut(s) 774, 835, 941
Tsp45I GTSAC 2 cut(s) 36, 94
TspDTI ATGAA 7 cut(s) 197, 266, 747, 1083, 1185, 1253, 1322
TspRI CASTG 3 cut(s) 787, 837, 1138
Van91I CCANNNNNTGG 1 cut(s) 324
XapI RAATTY 2 cut(s) 230, 1376
XbaI TCTAGA 2 cut(s) 1165, 1283
XceI RCATGY 2 cut(s) 50, 413
XhoI CTCGAG 2 cut(s) 793, 825
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 4 cut(s) 814, 817, 930, 1244
XspI CTAG 3 cut(s) 543, 1166, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.