AT1G62530

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006281
Physical Location & Seq
Reverse (-)
23146200 .. 23148367
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G62530.1

Sequence Viewer

Length: 939 bp
ATGTATCTCCACGGTTTGTCCAAGGACGAAATTTTGTGCAAGAAACGTTTTGAATCGTATGAAGAAAGTTCTAAGAAAAATCAAATTAGAATGGTCACTCTTTTTGGGCAGCAGGTGTATACAGCAAGAGACGAGAGCTTTGTGTCGTGTAATGAGCAGGTGAATAGAAACTGCCTTAATCCACTGATGGAAGATTGTGATCTTCAACTTCCTGAAAATCATGAAAGCGTCGAATACCGAGAATTCCACAGCATTGGTTCTTCCCAAGATAGCATAGATTTGAGGATTGAAGCATATGGCACAAATTTGTGTGGCAAGAAAAGTGAGAAAGTGAATATTTCAGATGGATCATGTTTGCTGGATCTTAATAAACCCCCGGCTTACGAGCCGGTTTCAATTGATCATCAATGCGGTTTAGATGGTGTACATGAAAAAGATTGTTCAGCATCTCCAGCTTCATGTTGCACCGCAGAAAACAATTCAAGGACTGAAGGAGAAGATTCGTGTGAAGTAATTCAAATGGCAGCTGAATGTTTAGTCCACATCTCAGCTGTTTCTCATAATCAAAGTCACGGTGTCCAAGAACCGGGTCGTTCTTGCGATTCATTTGAGCTACATACTTTAGAAATAAGAGAAACTGTCCCTGAAGAGCTCTGTTGTGTATCATCAAAGGCCATATATGACTTCAGTAAGAAGAAAGAGTTTGGAGTCAAGCTGAGAAGAGGGAGGAGAATGAAGAATTTCCAAAAGGAGATACTCCCGGAGCTTGTATCGCTCTCTAGACATGAGATTCGAGAAGATATTAACCTCTTAGAGACGGTTTTTAGATCGAGAGATTACAAGAAAATGCAGGGGAAGACTAAAGATGGAAAATGCAAACCAAACCTGAGAAACACCAAAGGTTTGACTCAAACGTATGTAGGTAAAAGAAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

312

Amino Acids

35.56

Weight (kDa)

6.39

Isoelectric Point (pI)

65.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 239 - 276 1e-09 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 103, 148
Acc36I ACCTGC 2 cut(s) 103, 148
AccI GTMKAC 1 cut(s) 119
AciI CCGC 2 cut(s) 411, 468
AclI AACGTT 1 cut(s) 46
AclWI GGATC 2 cut(s) 355, 369
AcsI RAATTY 4 cut(s) 30, 242, 304, 739
AcuI CTGAAG 3 cut(s) 510, 666, 670
AfaI GTAC 1 cut(s) 426
AfiI CCNNNNNNNGG 1 cut(s) 586
AgsI TTSAA 6 cut(s) 53, 206, 290, 396, 483, 518
AluBI AGCT 8 cut(s) 138, 455, 527, 551, 613, 652, 715, 766
AluI AGCT 8 cut(s) 138, 455, 527, 551, 613, 652, 715, 766
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 123, 809
AlwI GGATC 2 cut(s) 355, 369
AoxI GGCC 1 cut(s) 672
ApeKI GCWGC 2 cut(s) 109, 524
ApoI RAATTY 4 cut(s) 30, 242, 304, 739
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 774, 806
Asp700I GAANNNNTTC 2 cut(s) 513, 740
AsuC2I CCSGG 3 cut(s) 377, 588, 761
AsuHPI GGTGA 1 cut(s) 172
BanII GRGCYC 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 121, 536
BccI CCATC 4 cut(s) 181, 338, 413, 860
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 3 cut(s) 377, 588, 761
BcoDI GTCTC 2 cut(s) 123, 809
BfaI CTAG 1 cut(s) 780
BfuAI ACCTGC 2 cut(s) 103, 148
BisI GCNGC 2 cut(s) 110, 525
BlsI GCNGC 2 cut(s) 111, 526
Bme1390I CCNGG 3 cut(s) 377, 588, 761
BmrFI CCNGG 3 cut(s) 377, 588, 761
BmsI GCATC 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 435
BpuMI CCSGG 3 cut(s) 377, 588, 761
BsaBI GATNNNNATC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 10, 21, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse118I RCCGGY 1 cut(s) 388
Bse8I GATNNNNATC 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 10, 21, 375
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMII CTCAG 3 cut(s) 561, 707, 878
BseRI GAGGAG 1 cut(s) 742
BseXI GCAGC 2 cut(s) 121, 536
BshFI GGCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 654
BsiSI CCGG 4 cut(s) 377, 389, 587, 761
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 2 cut(s) 123, 809
BsmBI CGTCTC 2 cut(s) 123, 809
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 1 cut(s) 674
Bsp1286I GDGCHC 1 cut(s) 654
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 199, 347, 361, 400, 827
BspACI CCGC 2 cut(s) 411, 468
BspANI GGCC 1 cut(s) 674
BspCNI CTCAG 3 cut(s) 560, 708, 879
BspHI TCATGA 1 cut(s) 220
BspMI ACCTGC 2 cut(s) 103, 148
BspPI GGATC 2 cut(s) 355, 369
BspQI GCTCTTC 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 388
BsrGI TGTACA 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 10, 21, 375
BssMI GATC 5 cut(s) 199, 347, 361, 400, 827
BssNAI GTATAC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 21
Bst1107I GTATAC 1 cut(s) 120
Bst4CI ACNGT 4 cut(s) 14, 575, 640, 820
Bst6I CTCTTC 2 cut(s) 642, 715
BstAUI TGTACA 1 cut(s) 424
BstDEI CTNAG 5 cut(s) 72, 547, 716, 811, 887
BstDSI CCRYGG 1 cut(s) 10
BstKTI GATC 5 cut(s) 202, 350, 364, 403, 830
BstMAI GTCTC 2 cut(s) 123, 809
BstMBI GATC 5 cut(s) 199, 347, 361, 400, 827
BstMWI GCNNNNNNNGC 2 cut(s) 452, 772
BstSCI CCNGG 3 cut(s) 375, 586, 759
BstV1I GCAGC 2 cut(s) 121, 536
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 1 cut(s) 254
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 1 cut(s) 120
BsuRI GGCC 1 cut(s) 674
BtgI CCRYGG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 182
BveI ACCTGC 2 cut(s) 103, 148
CciI TCATGA 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 388
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 425
CviAII CATG 5 cut(s) 221, 351, 428, 459, 785
CviQI GTAC 1 cut(s) 425
DdeI CTNAG 5 cut(s) 72, 547, 716, 811, 887
DpnI GATC 5 cut(s) 201, 349, 363, 402, 829
DpnII GATC 5 cut(s) 199, 347, 361, 400, 827
Eam1104I CTCTTC 2 cut(s) 642, 715
EarI CTCTTC 2 cut(s) 642, 715
Ecl136II GAGCTC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 652
Eco57I CTGAAG 3 cut(s) 510, 666, 670
EcoICRI GAGCTC 1 cut(s) 652
EcoRI GAATTC 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 21
Esp3I CGTCTC 2 cut(s) 123, 809
FaeI CATG 5 cut(s) 224, 354, 431, 462, 788
FaqI GGGAC 1 cut(s) 626
FatI CATG 5 cut(s) 220, 350, 427, 458, 784
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 110, 525
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 2 cut(s) 110, 525
FspBI CTAG 1 cut(s) 780
GluI GCNGC 2 cut(s) 110, 525
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 674
HapII CCGG 4 cut(s) 377, 389, 587, 761
HgaI GACGC 1 cut(s) 217
Hin1II CATG 5 cut(s) 224, 354, 431, 462, 788
HinfI GANTC 6 cut(s) 53, 500, 602, 708, 790, 907
HpaII CCGG 4 cut(s) 377, 389, 587, 761
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 3 cut(s) 120, 425, 541
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 5 cut(s) 212, 221, 780, 794, 831
Hpy8I GTNNAC 3 cut(s) 120, 425, 541
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 485, 924
HpyCH4III ACNGT 4 cut(s) 14, 575, 640, 820
HpyCH4IV ACGT 2 cut(s) 46, 914
HpyCH4V TGCA 4 cut(s) 39, 465, 850, 876
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 772
HpyF3I CTNAG 5 cut(s) 72, 547, 716, 811, 887
HpySE526I ACGT 2 cut(s) 46, 914
Hsp92II CATG 5 cut(s) 224, 354, 431, 462, 788
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 199, 347, 361, 400, 827
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 1 cut(s) 763
Lsp1109I GCAGC 2 cut(s) 121, 536
LweI GCATC 1 cut(s) 455
MaeI CTAG 1 cut(s) 780
MaeII ACGT 2 cut(s) 46, 914
MaeIII GTNAC 2 cut(s) 94, 569
MalI GATC 5 cut(s) 201, 349, 363, 402, 829
MboI GATC 5 cut(s) 199, 347, 361, 400, 827
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 654
MluCI AATT 8 cut(s) 30, 84, 242, 304, 396, 478, 513, 739
MlyI GAGTC 2 cut(s) 717, 901
MnlI CCTC 4 cut(s) 276, 716, 720, 818
MroXI GAANNNNTTC 2 cut(s) 513, 740
MseI TTAA 3 cut(s) 177, 366, 804
MspA1I CMGCKG 2 cut(s) 527, 551
MspI CCGG 4 cut(s) 377, 389, 587, 761
MspR9I CCNGG 3 cut(s) 377, 588, 761
MunI CAATTG 1 cut(s) 396
MwoI GCNNNNNNNGC 2 cut(s) 452, 772
NciI CCSGG 3 cut(s) 377, 588, 761
NdeI CATATG 1 cut(s) 295
NdeII GATC 5 cut(s) 199, 347, 361, 400, 827
NlaIII CATG 5 cut(s) 224, 354, 431, 462, 788
NmuCI GTSAC 2 cut(s) 94, 569
PagI TCATGA 1 cut(s) 220
PaqCI CACCTGC 2 cut(s) 103, 148
PciSI GCTCTTC 1 cut(s) 642
PdmI GAANNNNTTC 2 cut(s) 513, 740
PfeI GAWTC 4 cut(s) 53, 500, 602, 790
PfoI TCCNGGA 1 cut(s) 759
PkrI GCNGC 2 cut(s) 111, 526
PleI GAGTC 2 cut(s) 716, 901
PpsI GAGTC 2 cut(s) 716, 901
Psp124BI GAGCTC 1 cut(s) 654
Psp1406I AACGTT 1 cut(s) 46
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 2 cut(s) 527, 551
RsaI GTAC 1 cut(s) 426
RsaNI GTAC 1 cut(s) 425
SacI GAGCTC 1 cut(s) 654
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 3 cut(s) 177, 366, 804
SatI GCNGC 2 cut(s) 110, 525
Sau3AI GATC 5 cut(s) 199, 347, 361, 400, 827
SchI GAGTC 2 cut(s) 717, 901
ScrFI CCNGG 3 cut(s) 377, 588, 761
SduI GDGCHC 1 cut(s) 654
SfaNI GCATC 1 cut(s) 455
Sse9I AATT 8 cut(s) 30, 84, 242, 304, 396, 478, 513, 739
SsiI CCGC 2 cut(s) 411, 468
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 780
SstI GAGCTC 1 cut(s) 654
StyD4I CCNGG 3 cut(s) 375, 586, 759
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 4 cut(s) 14, 575, 640, 820
TaiI ACGT 2 cut(s) 49, 917
TaqI TCGA 3 cut(s) 231, 793, 830
TasI AATT 8 cut(s) 30, 84, 242, 304, 396, 478, 513, 739
TatI WGTACW 1 cut(s) 424
TfiI GAWTC 4 cut(s) 53, 500, 602, 790
Tru1I TTAA 3 cut(s) 177, 366, 804
Tru9I TTAA 3 cut(s) 177, 366, 804
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 2 cut(s) 94, 569
TseI GCWGC 2 cut(s) 109, 524
Tsp45I GTSAC 2 cut(s) 94, 569
TspDTI ATGAA 6 cut(s) 75, 237, 444, 447, 594, 749
TspRI CASTG 1 cut(s) 189
XapI RAATTY 4 cut(s) 30, 242, 304, 739
XbaI TCTAGA 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 513, 740
XspI CTAG 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.