pycom06g06050

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006281
Physical Location & Seq
Forward (+)
9282943 .. 9283950
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g06050.1

Sequence Viewer

Length: 1008 bp
ATGGGCAATGTAAGCTTGGAGGCTGTGCCTAGATCGAAGATAGACCTCCAGGAAGCAGTTGGTTTCCACAGTAATGACCCCAGTAATGGAAACAATGGGTTCATCATGAAGCTCCTAAACAGTCGAAGTAGTTCGTGCACTTCTGCTAGACAAGTAAATTATGAGAACAACAGAGTAGAAGACCCTGTATTTTCATATACTGATCATTGTCGAAAAGTTGATCCAGATGGACATGGCAATATATCTTCTGCTTCATGTAAACCATGCTGCATTGGCAAAGATGATTCCAGAAGTGTAAAGACTATGCAGTCTGAAATTGAATATGGAAACTCAAATCCCTTTTCTGTTGATCAGATTTCAGGAACACATGTAGAGTCTCAAGTTTCAGAAACCTTAATGGGTGAGCAGGACCGAAGATCTTCTGACAGCAGTGAATTAAAACACAAAGACCTCAACAAGGGAGGGGATTCAGCTGAACTGGATGTTTTGATCAGAGCAGCTGAAGCACTTGTCGGAATCTCATTGGAGAGTTCATCATGTTGTCATGATTGCACTTACGAAGTAGGAGGTGCAAATAAGATGGAAACGAAGGAGAGTGAACAACCGCAGTGTTCTTCTGATTCTTATGAGTTACTCACACTAAATCTAATAGAGAGCACTGAAGATGACGATTGTGTGTCATTAAAGCCTTCTGAGGTGAATTACAATGACAGTAAGGAATTTGGCATTAAGTTGAGAAGGGGGAGGAGAATGAAAGATTTTCAGAGGGACATTCTTCCTGGTCTGGCATCTCTTTCCAGACACGAAATTCGTGAAGATATTAACATTATGGAGACAGTTTTAAGATCAAGAGAGTACAAAAAGCTAAGAGGCAAGGTAAGGTGGCTGATTATCAGAGCTGGTGTACTCCTGTCAAAAACAAACGTTCACGACTCAGCTATGTTCCGCGGAGGAAAAGAAATTTATGAGAAGCTTAAGTTGGGAATGACAGAGATTGTGGAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

336

Amino Acids

37.33

Weight (kDa)

5.43

Isoelectric Point (pI)

57.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 195 - 282 2e-16 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
AccII CGCG 1 cut(s) 948
AciI CCGC 3 cut(s) 605, 946, 948
AclI AACGTT 1 cut(s) 924
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 3 cut(s) 719, 807, 960
AcuI CTGAAG 2 cut(s) 522, 681
AfaI GTAC 2 cut(s) 857, 906
AfiI CCNNNNNNNGG 2 cut(s) 86, 457
AflII CTTAAG 1 cut(s) 974
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 1 cut(s) 320
AjnI CCWGG 2 cut(s) 48, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 962, 994
AluBI AGCT 8 cut(s) 15, 112, 473, 500, 865, 899, 938, 973
AluI AGCT 8 cut(s) 15, 112, 473, 500, 865, 899, 938, 973
Alw21I GWGCWC 2 cut(s) 140, 659
Alw26I GTCTC 2 cut(s) 381, 827
Alw44I GTGCAC 1 cut(s) 136
AlwI GGATC 1 cut(s) 215
ApaLI GTGCAC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 267, 497
ApoI RAATTY 3 cut(s) 719, 807, 960
ArsI GACNNNNNNTTYG 2 cut(s) 792, 824
Asp700I GAANNNNTTC 2 cut(s) 130, 418
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 2 cut(s) 413, 709
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 2 cut(s) 140, 659
BbvI GCAGC 2 cut(s) 254, 509
BccI CCATC 2 cut(s) 221, 574
BciT130I CCWGG 2 cut(s) 50, 780
BclI TGATCA 3 cut(s) 202, 349, 489
BcoDI GTCTC 2 cut(s) 381, 827
BfaI CTAG 2 cut(s) 30, 147
BfrI CTTAAG 1 cut(s) 974
BglII AGATCT 1 cut(s) 416
BisI GCNGC 2 cut(s) 268, 498
BlsI GCNGC 2 cut(s) 269, 499
Bme1390I CCNGG 2 cut(s) 50, 780
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 409
BmrFI CCNGG 2 cut(s) 50, 780
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 946
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 457
Bse1I ACTGG 2 cut(s) 81, 483
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 2 cut(s) 50, 780
BseDI CCNNGG 1 cut(s) 946
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 2 cut(s) 86, 457
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 684, 948
BseNI ACTGG 2 cut(s) 81, 483
BseRI GAGGAG 1 cut(s) 760
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 2 cut(s) 254, 509
Bsh1236I CGCG 1 cut(s) 948
BsiHKAI GWGCWC 2 cut(s) 140, 659
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 2 cut(s) 86, 457
BsmAI GTCTC 2 cut(s) 381, 827
BsmFI GGGAC 1 cut(s) 782
Bsp1286I GDGCHC 2 cut(s) 140, 659
Bsp143I GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
BspACI CCGC 3 cut(s) 605, 946, 948
BspCNI CTCAG 2 cut(s) 685, 947
BspFNI CGCG 1 cut(s) 948
BspHI TCATGA 2 cut(s) 105, 544
BspPI GGATC 1 cut(s) 215
BspTI CTTAAG 1 cut(s) 974
BsrDI GCAATG 1 cut(s) 13
BsrI ACTGG 2 cut(s) 81, 483
BssECI CCNNGG 1 cut(s) 946
BssMI GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
Bst2UI CCWGG 2 cut(s) 50, 780
Bst4CI ACNGT 4 cut(s) 71, 122, 713, 838
BstAFI CTTAAG 1 cut(s) 974
BstDEI CTNAG 3 cut(s) 693, 866, 934
BstDSI CCRYGG 1 cut(s) 946
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 1 cut(s) 487
BstFNI CGCG 1 cut(s) 948
BstKTI GATC 7 cut(s) 35, 205, 223, 352, 419, 492, 848
BstMAI GTCTC 2 cut(s) 381, 827
BstMBI GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
BstMWI GCNNNNNNNGC 3 cut(s) 12, 273, 503
BstNI CCWGG 2 cut(s) 50, 780
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 48, 778
BstSLI GKGCMC 1 cut(s) 140
BstUI CGCG 1 cut(s) 948
BstV1I GCAGC 2 cut(s) 254, 509
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BtgI CCRYGG 1 cut(s) 946
BtsCI GGATG 1 cut(s) 487
BtsI GCAGTG 2 cut(s) 436, 614
BtsIMutI CAGTG 3 cut(s) 436, 614, 657
CciI TCATGA 2 cut(s) 105, 544
Cfr13I GGNCC 1 cut(s) 409
Cfr42I CCGCGG 1 cut(s) 949
Csp6I GTAC 2 cut(s) 856, 905
CviAII CATG 7 cut(s) 106, 233, 255, 264, 368, 537, 545
CviQI GTAC 2 cut(s) 856, 905
DdeI CTNAG 3 cut(s) 693, 866, 934
DpnI GATC 7 cut(s) 34, 204, 222, 351, 418, 491, 847
DpnII GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 522, 681
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 48, 778
FaeI CATG 7 cut(s) 109, 236, 258, 267, 371, 540, 548
FaqI GGGAC 1 cut(s) 782
FatI CATG 7 cut(s) 105, 232, 254, 263, 367, 536, 544
FbaI TGATCA 3 cut(s) 202, 349, 489
Fnu4HI GCNGC 2 cut(s) 268, 498
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 2 cut(s) 268, 498
FspBI CTAG 2 cut(s) 30, 147
GluI GCNGC 2 cut(s) 268, 498
GsuI CTGGAG 1 cut(s) 32
Hin1II CATG 7 cut(s) 109, 236, 258, 267, 371, 540, 548
HindIII AAGCTT 2 cut(s) 13, 971
HinfI GANTC 6 cut(s) 284, 374, 467, 516, 620, 932
HphI GGTGA 2 cut(s) 413, 709
Hpy166II GTNNAC 5 cut(s) 138, 260, 599, 905, 928
Hpy188III TCNNGA 9 cut(s) 106, 224, 288, 360, 545, 798, 812, 849, 929
Hpy8I GTNNAC 5 cut(s) 138, 260, 599, 905, 928
HpyAV CCTTC 3 cut(s) 583, 699, 732
HpyCH4III ACNGT 4 cut(s) 71, 122, 713, 838
HpyCH4IV ACGT 1 cut(s) 924
HpyCH4V TGCA 5 cut(s) 138, 270, 307, 552, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 273, 503
HpyF3I CTNAG 3 cut(s) 693, 866, 934
HpySE526I ACGT 1 cut(s) 924
Hsp92II CATG 7 cut(s) 109, 236, 258, 267, 371, 540, 548
Ksp22I TGATCA 3 cut(s) 202, 349, 489
KspI CCGCGG 1 cut(s) 949
Kzo9I GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 2 cut(s) 254, 509
LweI GCATC 1 cut(s) 797
MaeI CTAG 2 cut(s) 30, 147
MaeII ACGT 1 cut(s) 924
MaeIII GTNAC 1 cut(s) 630
MalI GATC 7 cut(s) 34, 204, 222, 351, 418, 491, 847
MboI GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
MboII GAAGA 9 cut(s) 49, 191, 237, 411, 426, 606, 674, 767, 827
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 140, 659
MluCI AATT 7 cut(s) 157, 315, 434, 700, 719, 807, 960
MlyI GAGTC 2 cut(s) 383, 926
MmeI TCCRAC 1 cut(s) 493
MroXI GAANNNNTTC 2 cut(s) 130, 418
MseI TTAA 7 cut(s) 395, 437, 683, 729, 822, 842, 975
MslI CAYNNNNRTG 1 cut(s) 72
MspA1I CMGCKG 3 cut(s) 473, 500, 948
MspCI CTTAAG 1 cut(s) 974
MspR9I CCNGG 2 cut(s) 50, 780
MvaI CCWGG 2 cut(s) 50, 780
MvnI CGCG 1 cut(s) 948
MwoI GCNNNNNNNGC 3 cut(s) 12, 273, 503
NdeII GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
NlaIII CATG 7 cut(s) 109, 236, 258, 267, 371, 540, 548
NspI RCATGY 1 cut(s) 371
PagI TCATGA 2 cut(s) 105, 544
PciI ACATGT 1 cut(s) 367
PdmI GAANNNNTTC 2 cut(s) 130, 418
PfeI GAWTC 4 cut(s) 284, 467, 516, 620
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 2 cut(s) 269, 499
PleI GAGTC 2 cut(s) 382, 926
PpsI GAGTC 2 cut(s) 382, 926
PscI ACATGT 1 cut(s) 367
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 48, 778
PspGI CCWGG 2 cut(s) 48, 778
PspPI GGNCC 1 cut(s) 409
PsuI RGATCY 1 cut(s) 416
PvuII CAGCTG 2 cut(s) 473, 500
RsaI GTAC 2 cut(s) 857, 906
RsaNI GTAC 2 cut(s) 856, 905
RseI CAYNNNNRTG 1 cut(s) 72
SacII CCGCGG 1 cut(s) 949
SaqAI TTAA 7 cut(s) 395, 437, 683, 729, 822, 842, 975
SatI GCNGC 2 cut(s) 268, 498
Sau3AI GATC 7 cut(s) 32, 202, 220, 349, 416, 489, 845
Sau96I GGNCC 1 cut(s) 409
SchI GAGTC 2 cut(s) 383, 926
ScrFI CCNGG 2 cut(s) 50, 780
SduI GDGCHC 2 cut(s) 140, 659
SfaNI GCATC 1 cut(s) 797
Sfr303I CCGCGG 1 cut(s) 949
SgrBI CCGCGG 1 cut(s) 949
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 72
SmlI CTYRAG 2 cut(s) 378, 974
SmoI CTYRAG 2 cut(s) 378, 974
Sse9I AATT 7 cut(s) 157, 315, 434, 700, 719, 807, 960
SsiI CCGC 3 cut(s) 605, 946, 948
SspMI CTAG 2 cut(s) 30, 147
StyD4I CCNGG 2 cut(s) 48, 778
TaaI ACNGT 4 cut(s) 71, 122, 713, 838
TaiI ACGT 1 cut(s) 927
TaqI TCGA 3 cut(s) 35, 124, 211
TaqII GACCGA 1 cut(s) 426
TasI AATT 7 cut(s) 157, 315, 434, 700, 719, 807, 960
TatI WGTACW 2 cut(s) 855, 904
TfiI GAWTC 4 cut(s) 284, 467, 516, 620
Tru1I TTAA 7 cut(s) 395, 437, 683, 729, 822, 842, 975
Tru9I TTAA 7 cut(s) 395, 437, 683, 729, 822, 842, 975
TscAI CASTG 3 cut(s) 436, 614, 664
TseI GCWGC 2 cut(s) 267, 497
TspDTI ATGAA 6 cut(s) 91, 122, 183, 243, 522, 767
TspRI CASTG 3 cut(s) 436, 614, 664
Vha464I CTTAAG 1 cut(s) 974
VneI GTGCAC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 409
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 3 cut(s) 719, 807, 960
XceI RCATGY 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmnI GAANNNNTTC 2 cut(s) 130, 418
XspI CTAG 2 cut(s) 30, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.