FvH4_5g00200

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006281
Physical Location & Seq
Reverse (-)
142709 .. 146440
3732 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00200.t3

Sequence Viewer

Length: 1806 bp
ATGTTGATGCGAGGAGATTTTGATCTGAACTCGATACAAGCTTATTCGGGTTCTCTGAACGATGTCTTGAGGAACACTATGCTGAACCAGGAGCTGGTATTTAAAGACCAGGTTCATGAACTCCACCATTTATATAGGACACAGAGAACACTGATGCAGAATCTCGACCAGAAAGAGCATCATAGATACAATGTCAGCAACGCAAGTGCTCAGTCAAATCTGTCACATTGGACAAATTCTACAAGAGATGAACCAAGGGACTTGCTAGAAGGACACAAGAGTATATGTGATAATAGTCAGCTGAGGCCTCCTGGTCCTAGACATCTCTTAGATCCATGCAACAAACATATTGATCCAGCAGAGCTGAAGCTTTCTCTGAGAGCTCGAGAGGGCAGCAGAAGGGAGGGAAGCACTAAGAGAAATTGGTTTGATGCAGGATCTCATTCCTCCTCACCAATTATGATTGATTTAGAAGAAGATGAAAGTGACTGGAATCCTTTAGATCAAGGATTAAATAGGTGTTTTGGTGGGATCTCAATTAACAAGTCATCCACCAGCCAACAGTTCAGTTCTTGTGAAGCAAGGCTTCTCGACCTAAATAAAACCCAGCCCGATGATTCTTCTTGTCTCTCAAGTGATCCTACGGTAGTCCATCCTTCAACAGCAAGCTCATCGCATGCTTTCCATGGGGTAGCTGGCAAGATAAAAGAAAGCACTGTCTGTTTCACTACTGTGAAAAAGGATCATGGTGGAAATCTTTCTTCATTTTCCACCAGGGAAAATGAGAAAGCAGAATATTTGGGCAGGAAGTTGAAAGATGATGGATTCAGAATAAGTCAAATGGACCCTGTAACTTTGGAGAGAGCCGTGTTTAGCTCCAAATCAGATCTTTGTGAAGCAGTTGGCTGCAACAGCACTGACCGTAAAAATGGAACTGATGGGTTCATCATGAAACTCCTGAACAGTCGAACCCATTCATGTGCAGCTTCTGAACAAGCAAATTTGGTAAACACCAAAGTAGAAGACTCTGCATTTCCATATTTTGGTTATAAGAAAACAACAGATCAAGATGGACACGGCAATATATCTTCTTCTGCAAGTAAGCCGTATTGTATCGGTGATAACGATTCCAGTAGTGTGAAGACCATACTATCTGACATTGAGACCAGAAACTCAAACCCTTTTTCTTCCGATCAGTTTTCAGCAACATATGTGGGGTCTCAAGTTTCTGAAACTTTTGTGGGTGAGCAGAACCAAATATCTACAGACAGCAGTGAATCTAAACACAAATGCATCAACAGGGAAGAAGAATCAGCTGAAGTGAATGCTTTGACCAGAGAGGCAGCTGAAGCACTTCTCGGAATGTCATTGGACAGCTCCTTTTGTTATAAAGATTGTGCTGGTAACGTGGGAGAATTAAACAAGTTCGATAAGGAGAGTGAGAAACCACAGTATACTTGTGATTTTTTTGAGTTAATGACACTAGCACTTACGGAGAGCAGTGTGGATGACTATTCTGTGTCTTCAAATCCTTCTGAATCGTGTTTCACAGAAAATAAGGATGTTGGTCTAAAGTTGAGAAGGGGAAGGAGATTAAAAGATTTTCAGAAGGACATACTTCCTGGTTTGGCATCTCTTTCCAGGCATGAAATTCGAGAAGATATAAACATTATGGAGGGAGTTCTAAGGTCAAGAGAGTACAAGAGATTACAGGCCAAAATCGCTGATGGCCACGGCTGGTGTACTCCTGTCAGAAACAAACGTTCGCGACTGAACCGTGTCACGCAGAGCAAAAAATTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

602

Amino Acids

67.22

Weight (kDa)

6.05

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 478 - 563 1.8e-20 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1050, 1389
AccB7I CCANNNNNTGG 2 cut(s) 94, 1043
AccI GTMKAC 1 cut(s) 1454
AccII CGCG 1 cut(s) 1768
AclI AACGTT 1 cut(s) 1762
AclWI GGATC 6 cut(s) 326, 347, 445, 539, 632, 750
AcoI YGGCCR 1 cut(s) 1729
AcsI RAATTY 4 cut(s) 235, 1000, 1650, 1796
AcuI CTGAAG 3 cut(s) 386, 1338, 1368
AfaI GTAC 2 cut(s) 1700, 1744
AfiI CCNNNNNNNGG 2 cut(s) 94, 1043
AgsI TTSAA 3 cut(s) 660, 814, 1527
AjnI CCWGG 6 cut(s) 87, 108, 310, 773, 1621, 1640
AleI CACNNNNGTG 1 cut(s) 731
Alw21I GWGCWC 2 cut(s) 211, 385
Alw26I GTCTC 3 cut(s) 632, 1157, 1224
AlwI GGATC 6 cut(s) 326, 347, 445, 539, 632, 750
AlwNI CAGNNNCTG 2 cut(s) 94, 989
Ama87I CYCGRG 1 cut(s) 384
AoxI GGCC 3 cut(s) 305, 1713, 1729
ApeKI GCWGC 4 cut(s) 393, 906, 983, 1343
ApoI RAATTY 4 cut(s) 235, 1000, 1650, 1796
Asp700I GAANNNNTTC 3 cut(s) 757, 973, 1353
AspS9I GGNCC 2 cut(s) 314, 844
AsuHPI GGTGA 3 cut(s) 444, 1130, 1256
AvaI CYCGRG 1 cut(s) 384
AvaII GGWCC 2 cut(s) 314, 844
BalI TGGCCA 1 cut(s) 1731
BanII GRGCYC 1 cut(s) 385
BbsI GAAGAC 3 cut(s) 1029, 1148, 1515
Bbv12I GWGCWC 2 cut(s) 211, 385
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 4 cut(s) 405, 893, 995, 1355
BccI CCATC 5 cut(s) 660, 815, 932, 1064, 1721
BceAI ACGGC 4 cut(s) 851, 1090, 1093, 1750
BcgI CGANNNNNNTGC 4 cut(s) 654, 688, 1634, 1668
BciT130I CCWGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
BcoDI GTCTC 3 cut(s) 632, 1157, 1224
BfaI CTAG 3 cut(s) 266, 318, 1484
BfmI CTRYAG 1 cut(s) 1263
BglII AGATCT 1 cut(s) 886
BisI GCNGC 4 cut(s) 394, 907, 984, 1344
BlsI GCNGC 4 cut(s) 395, 908, 985, 1345
Bme1390I CCNGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
Bme18I GGWCC 2 cut(s) 314, 844
BmeT110I CYCGRG 1 cut(s) 384
BmgT120I GGNCC 2 cut(s) 314, 844
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
BmsI GCATC 5 cut(s) 144, 187, 421, 1302, 1640
BpiI GAAGAC 3 cut(s) 1029, 1148, 1515
Bpu10I CCTNAGC 1 cut(s) 302
BpuEI CTTGAG 3 cut(s) 88, 616, 1206
BsaBI GATNNNNATC 1 cut(s) 21
BsaI GGTCTC 2 cut(s) 1157, 1224
BsaJI CCNNGG 4 cut(s) 254, 685, 774, 1732
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 1043
Bse1I ACTGG 2 cut(s) 494, 1131
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
BseDI CCNNGG 4 cut(s) 254, 685, 774, 1732
BseGI GGATG 4 cut(s) 548, 652, 1513, 1567
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 94, 1043
BseMII CTCAG 3 cut(s) 224, 293, 368
BseNI ACTGG 2 cut(s) 494, 1131
BseRI GAGGAG 2 cut(s) 27, 439
BseXI GCAGC 4 cut(s) 405, 893, 995, 1355
BseYI CCCAGC 1 cut(s) 606
BsgI GTGCAG 1 cut(s) 1002
Bsh1236I CGCG 1 cut(s) 1768
BshFI GGCC 3 cut(s) 307, 1715, 1731
BsiHKAI GWGCWC 2 cut(s) 211, 385
BsiHKCI CYCGRG 1 cut(s) 384
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 2 cut(s) 94, 1043
BsmAI GTCTC 3 cut(s) 632, 1157, 1224
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 1330
BsnI GGCC 3 cut(s) 307, 1715, 1731
Bso31I GGTCTC 2 cut(s) 1157, 1224
BsoBI CYCGRG 1 cut(s) 384
Bsp1286I GDGCHC 2 cut(s) 211, 385
Bsp19I CCATGG 1 cut(s) 685
Bsp68I TCGCGA 1 cut(s) 1768
BspANI GGCC 3 cut(s) 307, 1715, 1731
BspCNI CTCAG 3 cut(s) 223, 294, 369
BspFNI CGCG 1 cut(s) 1768
BspHI TCATGA 2 cut(s) 115, 948
BspLI GGNNCC 1 cut(s) 846
BspPI GGATC 6 cut(s) 326, 347, 445, 539, 632, 750
BspTNI GGTCTC 2 cut(s) 1157, 1224
BsrI ACTGG 2 cut(s) 494, 1131
BssECI CCNNGG 4 cut(s) 254, 685, 774, 1732
BssNAI GTATAC 1 cut(s) 1455
BssT1I CCWWGG 2 cut(s) 254, 685
Bst1107I GTATAC 1 cut(s) 1455
Bst2UI CCWGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
Bst4CI ACNGT 8 cut(s) 564, 646, 718, 733, 923, 965, 1452, 1778
BstC8I GCNNGC 3 cut(s) 667, 678, 697
BstDEI CTNAG 6 cut(s) 210, 302, 328, 377, 414, 1685
BstDSI CCRYGG 2 cut(s) 685, 1732
BstF5I GGATG 4 cut(s) 548, 652, 1513, 1567
BstFNI CGCG 1 cut(s) 1768
BstMAI GTCTC 3 cut(s) 632, 1157, 1224
BstMWI GCNNNNNNNGC 3 cut(s) 912, 1349, 1721
BstNI CCWGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
BstNSI RCATGY 1 cut(s) 680
BstSCI CCNGG 6 cut(s) 87, 108, 310, 773, 1621, 1640
BstSFI CTRYAG 1 cut(s) 1263
BstUI CGCG 1 cut(s) 1768
BstV1I GCAGC 4 cut(s) 405, 893, 995, 1355
BstV2I GAAGAC 3 cut(s) 1029, 1148, 1515
BstX2I RGATCY 4 cut(s) 331, 437, 531, 886
BstYI RGATCY 4 cut(s) 331, 437, 531, 886
BstZ17I GTATAC 1 cut(s) 1455
BsuRI GGCC 3 cut(s) 307, 1715, 1731
BtgI CCRYGG 2 cut(s) 685, 1732
BtgZI GCGATG 1 cut(s) 657
BtsCI GGATG 4 cut(s) 548, 652, 1513, 1567
BtsI GCAGTG 2 cut(s) 1279, 1507
BtsIMutI CAGTG 5 cut(s) 149, 714, 915, 1279, 1507
BtuMI TCGCGA 1 cut(s) 1768
Cac8I GCNNGC 3 cut(s) 667, 678, 697
CaiI CAGNNNCTG 2 cut(s) 94, 989
CciI TCATGA 2 cut(s) 115, 948
Cfr13I GGNCC 2 cut(s) 314, 844
CsiI ACCWGGT 1 cut(s) 108
Csp6I GTAC 2 cut(s) 1699, 1743
CspCI CAANNNNNGTGG 2 cut(s) 1194, 1229
CviAII CATG 8 cut(s) 116, 336, 677, 686, 746, 949, 978, 1646
CviQI GTAC 2 cut(s) 1699, 1743
DdeI CTNAG 6 cut(s) 210, 302, 328, 377, 414, 1685
DraI TTTAAA 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 1729
Ecl136II GAGCTC 1 cut(s) 383
Eco130I CCWWGG 2 cut(s) 254, 685
Eco147I AGGCCT 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 2 cut(s) 1157, 1224
Eco47I GGWCC 2 cut(s) 314, 844
Eco53kI GAGCTC 1 cut(s) 383
Eco57I CTGAAG 3 cut(s) 386, 1338, 1368
Eco88I CYCGRG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 383
EcoRII CCWGG 6 cut(s) 87, 108, 310, 773, 1621, 1640
EcoT14I CCWWGG 2 cut(s) 254, 685
EcoT22I ATGCAT 1 cut(s) 1295
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 254, 685
FaeI CATG 8 cut(s) 119, 339, 680, 689, 749, 952, 981, 1649
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 272
FatI CATG 8 cut(s) 115, 335, 676, 685, 745, 948, 977, 1645
FauNDI CATATG 1 cut(s) 1210
FblI GTMKAC 1 cut(s) 1454
Fnu4HI GCNGC 4 cut(s) 394, 907, 984, 1344
FokI GGATG 4 cut(s) 535, 639, 1520, 1574
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 4 cut(s) 394, 907, 984, 1344
FspBI CTAG 3 cut(s) 266, 318, 1484
GluI GCNGC 4 cut(s) 394, 907, 984, 1344
GsaI CCCAGC 1 cut(s) 610
HaeIII GGCC 3 cut(s) 307, 1715, 1731
Hin1II CATG 8 cut(s) 119, 339, 680, 689, 749, 952, 981, 1649
HindIII AAGCTT 2 cut(s) 39, 368
HinfI GANTC 9 cut(s) 160, 493, 617, 825, 1025, 1127, 1277, 1310, 1538
HphI GGTGA 3 cut(s) 444, 1130, 1256
Hpy166II GTNNAC 3 cut(s) 1009, 1455, 1743
Hpy8I GTNNAC 3 cut(s) 1009, 1455, 1743
HpyAV CCTTC 7 cut(s) 263, 393, 666, 1542, 1575, 1581, 1603
HpyCH4III ACNGT 8 cut(s) 564, 646, 718, 733, 923, 965, 1452, 1778
HpyCH4IV ACGT 2 cut(s) 1407, 1762
HpyCH4V TGCA 8 cut(s) 157, 339, 434, 909, 983, 1031, 1097, 1293
HpyF10VI GCNNNNNNNGC 3 cut(s) 912, 1349, 1721
HpyF3I CTNAG 6 cut(s) 210, 302, 328, 377, 414, 1685
HpySE526I ACGT 2 cut(s) 1407, 1762
Hsp92II CATG 8 cut(s) 119, 339, 680, 689, 749, 952, 981, 1649
LmnI GCTCC 3 cut(s) 91, 881, 1382
Lsp1109I GCAGC 4 cut(s) 405, 893, 995, 1355
LweI GCATC 5 cut(s) 144, 187, 421, 1302, 1640
MabI ACCWGGT 1 cut(s) 108
MaeI CTAG 3 cut(s) 266, 318, 1484
MaeII ACGT 2 cut(s) 1407, 1762
MaeIII GTNAC 5 cut(s) 222, 485, 850, 1403, 1780
MflI RGATCY 4 cut(s) 331, 437, 531, 886
MhlI GDGCHC 2 cut(s) 211, 385
MlsI TGGCCA 1 cut(s) 1731
MluCI AATT 8 cut(s) 235, 421, 456, 537, 1000, 1415, 1650, 1796
MluNI TGGCCA 1 cut(s) 1731
MlyI GAGTC 1 cut(s) 1019
Mox20I TGGCCA 1 cut(s) 1731
Mph1103I ATGCAT 1 cut(s) 1295
MroXI GAANNNNTTC 3 cut(s) 757, 973, 1353
MscI TGGCCA 1 cut(s) 1731
MseI TTAA 6 cut(s) 102, 512, 540, 1418, 1475, 1595
MslI CAYNNNNRTG 2 cut(s) 731, 978
Msp20I TGGCCA 1 cut(s) 1731
MspA1I CMGCKG 3 cut(s) 301, 1316, 1346
MspR9I CCNGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
Mva1269I GAATGC 1 cut(s) 1330
MvaI CCWGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
MvnI CGCG 1 cut(s) 1768
MwoI GCNNNNNNNGC 3 cut(s) 912, 1349, 1721
NcoI CCATGG 1 cut(s) 685
NdeI CATATG 1 cut(s) 1210
NlaIII CATG 8 cut(s) 119, 339, 680, 689, 749, 952, 981, 1649
NlaIV GGNNCC 1 cut(s) 846
NmuCI GTSAC 3 cut(s) 222, 485, 1780
NruI TCGCGA 1 cut(s) 1768
NsiI ATGCAT 1 cut(s) 1295
NspI RCATGY 1 cut(s) 680
OliI CACNNNNGTG 1 cut(s) 731
PaeI GCATGC 1 cut(s) 680
PaeR7I CTCGAG 1 cut(s) 384
PagI TCATGA 2 cut(s) 115, 948
PceI AGGCCT 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 1122
PctI GAATGC 1 cut(s) 1330
PdmI GAANNNNTTC 3 cut(s) 757, 973, 1353
PfeI GAWTC 8 cut(s) 160, 493, 617, 825, 1127, 1277, 1310, 1538
PflMI CCANNNNNTGG 2 cut(s) 94, 1043
PkrI GCNGC 4 cut(s) 395, 908, 985, 1345
PleI GAGTC 1 cut(s) 1019
PpsI GAGTC 1 cut(s) 1019
PsiI TTATAA 2 cut(s) 1050, 1389
Psp124BI GAGCTC 1 cut(s) 385
Psp1406I AACGTT 1 cut(s) 1762
Psp6I CCWGG 6 cut(s) 87, 108, 310, 773, 1621, 1640
PspFI CCCAGC 1 cut(s) 606
PspGI CCWGG 6 cut(s) 87, 108, 310, 773, 1621, 1640
PspN4I GGNNCC 1 cut(s) 846
PspPI GGNCC 2 cut(s) 314, 844
PstNI CAGNNNCTG 2 cut(s) 94, 989
PsuI RGATCY 4 cut(s) 331, 437, 531, 886
PvuII CAGCTG 3 cut(s) 301, 1316, 1346
RruI TCGCGA 1 cut(s) 1768
RsaI GTAC 2 cut(s) 1700, 1744
RsaNI GTAC 2 cut(s) 1699, 1743
RseI CAYNNNNRTG 2 cut(s) 731, 978
SacI GAGCTC 1 cut(s) 385
SaqAI TTAA 6 cut(s) 102, 512, 540, 1418, 1475, 1595
SatI GCNGC 4 cut(s) 394, 907, 984, 1344
Sau96I GGNCC 2 cut(s) 314, 844
SchI GAGTC 1 cut(s) 1019
ScrFI CCNGG 6 cut(s) 89, 110, 312, 775, 1623, 1642
SduI GDGCHC 2 cut(s) 211, 385
SexAI ACCWGGT 1 cut(s) 108
SfaNI GCATC 5 cut(s) 144, 187, 421, 1302, 1640
SfcI CTRYAG 1 cut(s) 1263
Sfr274I CTCGAG 1 cut(s) 384
SinI GGWCC 2 cut(s) 314, 844
SlaI CTCGAG 1 cut(s) 384
SmiMI CAYNNNNRTG 2 cut(s) 731, 978
SmlI CTYRAG 4 cut(s) 67, 384, 631, 1221
SmoI CTYRAG 4 cut(s) 67, 384, 631, 1221
SphI GCATGC 1 cut(s) 680
Sse9I AATT 8 cut(s) 235, 421, 456, 537, 1000, 1415, 1650, 1796
SseBI AGGCCT 1 cut(s) 307
SspI AATATT 1 cut(s) 797
SspMI CTAG 3 cut(s) 266, 318, 1484
SstI GAGCTC 1 cut(s) 385
StuI AGGCCT 1 cut(s) 307
StyD4I CCNGG 6 cut(s) 87, 108, 310, 773, 1621, 1640
StyI CCWWGG 2 cut(s) 254, 685
TaaI ACNGT 8 cut(s) 564, 646, 718, 733, 923, 965, 1452, 1778
TaiI ACGT 2 cut(s) 1410, 1765
TaqI TCGA 7 cut(s) 32, 165, 385, 591, 967, 1428, 1654
TasI AATT 8 cut(s) 235, 421, 456, 537, 1000, 1415, 1650, 1796
TatI WGTACW 2 cut(s) 1698, 1742
TfiI GAWTC 8 cut(s) 160, 493, 617, 825, 1127, 1277, 1310, 1538
Tru1I TTAA 6 cut(s) 102, 512, 540, 1418, 1475, 1595
Tru9I TTAA 6 cut(s) 102, 512, 540, 1418, 1475, 1595
TscAI CASTG 5 cut(s) 156, 721, 922, 1279, 1507
TseFI GTSAC 3 cut(s) 222, 485, 1780
TseI GCWGC 4 cut(s) 393, 906, 983, 1343
Tsp45I GTSAC 3 cut(s) 222, 485, 1780
TspDTI ATGAA 9 cut(s) 104, 132, 264, 495, 753, 934, 965, 966, 1662
TspGWI ACGGA 1 cut(s) 1508
TspRI CASTG 5 cut(s) 156, 721, 922, 1279, 1507
Van91I CCANNNNNTGG 2 cut(s) 94, 1043
VpaK11BI GGWCC 2 cut(s) 314, 844
XapI RAATTY 4 cut(s) 235, 1000, 1650, 1796
XceI RCATGY 1 cut(s) 680
XcmI CCANNNNNNNNNTGG 1 cut(s) 692
XhoI CTCGAG 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 1454
XmnI GAANNNNTTC 3 cut(s) 757, 973, 1353
XspI CTAG 3 cut(s) 266, 318, 1484
Zsp2I ATGCAT 1 cut(s) 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.