MD04G1085400.v1.1

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006281
Physical Location & Seq
Forward (+)
12349520 .. 12354729
5210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1085400.v1.1.491

Sequence Viewer

Length: 1908 bp
ATGCTCAGCCAGGAGATTGTGTTTAAAAAGCAGGTTCATCAACTCCATCATCTATATAGGATACAGAAGACGCTGATGCAAAACCTTGGCTGGATGGAGTTTGGTAGATACAATTACAGCATAGCTGGAGCTGAGTCGAATCTTTTACCTCTGAAAAATTATGCTAGAGATGAACCTATGGAATTGTTAGAAGGGTGCAAGGGTGTAGATGATAAGATCCAGCCGAGGACTCCTGAGCTTAAACTTCCCTCAGACCAATTTCTTAGCTATATTGATCCAGCTGAGGTGAAGCTTTCCCTGAGCATTCAAGAGGACAGTAAGATAGAGGGAGGTACTATGAGGACTTGGTTTGATTTAGAAACTCATTCCGGCTCGCAACACGTGATTGATTTAGAAGAATCGATTGAAAGGACATCAAATGAGGGTTTGGAACAGTCACCTTCATTCAGCAGTACTGCTCCAATTACAAGCGTCGGGGGCACCCACAATTTAGAAGTTGCTGTCCTTTTTGATCCGATCATCTCAAGTAGTGTGAAGAAAAATCTATTGTTTCATGCTGCCGAGAGTTACCCTCATTTAGATGGCAGGGAGAGGAATCCTTTCAATCAAGGATTAGAAAAGTGTTCCGGTGGCATTGCCATTAACAATTCTTCCACCAGGAGGGAGCTGTTCGGTTCATATGAAACAGGGGTTCTGGACCTCAACAAAATTCAGCATGATGATTCATCTTGTCTCTCAAATGATCGTACAGTAGCCCATCTTTCAACCGCTAGCTCGTCACATTTACACGGATTAGTTGGCAAGGTAGAGGAGGGCACTCATTGTTTCGGAACTGGGAAAAAGAAAAATAGTAATAGCTCAATTGAAAGTTCTGAAAGGTTTAAAAAATACAATGCTGGAAATTTCAAAGTTGATGGATCGTGCATAAGTGGAATGGTCAATGAAAGCTTGGAGGCTGCGTCTAGATCAAAGATAGACCTCTGTGAAGCAGTTGGTTTCCACAGTAGTGACCCCAGTAATGGAAACAATGGGTTCATCATGAAACTCTTAAACAGTCGAAGTAGTTCGTGTACTTCTGCTAAACAAGTGAATTGTGAGAACAACAAAATAGAAGACCCCTTATTTCCATCTACTGATAATTGTCAAAAAACTGATCGAGATGGGCATGGCAATATATCTTCTGCTTCATGTAAACCATGCTGCATTGCCGAAGATGATTCCAGCAGCGGAAAGACTATGCAGTCTGAGATTGAATACAGAAACTCAAATCCCCTTTCCGTTGATCAGTTTTCAGGAACACATGTAGGGTCTCAAGTTTCAGAAACCTTAATGTGTGAGCAGGAGCAAAGATCTTCTGACAACAATGAATTAAAACACAAAGACCTCAACAAGGGAGGAGATTCAGCTGAACTGGATGTTTTGATCAGAGCAGCAGCTGAAGCACTTGTCGGAATCTCATTGGAGAGTTCATCCTGTTATCAAGATTGCGCTCACGAAGTAGGAGGTTCAACAAAGATGGAAACGAGGGAGAGTGAACAACCGCAGTGTTCTTCTGATTCTTATGAGTTACTCACACTAAATCTAACAGGGAGCACTGAAGATAACTATTGCGTGTCATCAAAGCCTTCTGAAGTAAATTACAACGACAGTAAGGAATTTGGCATTAAGTTGAGAAGGGGGAGGAGAATGAAAGATTTTCAGAGGGACATTCTTCCTGGTCTGGCTTCTCTTTCCAGACACGAAATCCGTGAAGATATTACCATTATGGAGACAGTTTTAAGATCAAGGGAGTACAAAAAATTAAGAGGCAAGGTGGTTGATGGTCAGAGCTGGTGTACTCCTGTCAAGAACAAACGTTCGCGACTCAACTATGTTCCGCGGAGGAAAAGAAATTTACGAGAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

636

Amino Acids

70.53

Weight (kDa)

5.82

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 490 - 594 1.6e-16 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1240
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 479
AccII CGCG 2 cut(s) 1861, 1879
AciI CCGC 5 cut(s) 768, 1227, 1541, 1877, 1879
AclI AACGTT 1 cut(s) 1855
AclWI GGATC 4 cut(s) 211, 269, 506, 925
AcsI RAATTY 4 cut(s) 708, 901, 1655, 1891
AcuI CTGAAG 3 cut(s) 1458, 1617, 1650
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 6 cut(s) 334, 454, 748, 1072, 1793, 1837
AfiI CCNNNNNNNGG 3 cut(s) 660, 1019, 1390
AflIII ACRYGT 2 cut(s) 379, 1300
AgsI TTSAA 8 cut(s) 308, 407, 604, 765, 866, 907, 1253, 1509
AjnI CCWGG 3 cut(s) 9, 656, 1714
AleI CACNNNNGTG 1 cut(s) 1005
Alw21I GWGCWC 1 cut(s) 1595
Alw26I GTCTC 3 cut(s) 737, 1314, 1763
AlwI GGATC 4 cut(s) 211, 269, 506, 925
AlwNI CAGNNNCTG 1 cut(s) 1436
ApeKI GCWGC 6 cut(s) 557, 956, 1200, 1224, 1430, 1433
ApoI RAATTY 4 cut(s) 708, 901, 1655, 1891
Asp700I GAANNNNTTC 2 cut(s) 599, 1063
AspLEI GCGC 1 cut(s) 1490
AspS9I GGNCC 1 cut(s) 697
AsuHPI GGTGA 2 cut(s) 298, 429
AsuNHI GCTAGC 1 cut(s) 770
AvaII GGWCC 1 cut(s) 697
BaeGI GKGCMC 2 cut(s) 482, 818
BanI GGYRCC 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 382
BbsI GAAGAC 2 cut(s) 74, 1119
Bbv12I GWGCWC 1 cut(s) 1595
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 6 cut(s) 544, 943, 1187, 1236, 1442, 1445
BccI CCATC 9 cut(s) 54, 88, 575, 765, 908, 1135, 1154, 1510, 1814
BciT130I CCWGG 3 cut(s) 11, 658, 1716
BciVI GTATCC 1 cut(s) 54
BclI TGATCA 2 cut(s) 1282, 1422
BcoDI GTCTC 3 cut(s) 737, 1314, 1763
BfaI CTAG 3 cut(s) 165, 771, 963
BfuAI ACCTGC 1 cut(s) 22
BfuI GTATCC 1 cut(s) 54
BglII AGATCT 1 cut(s) 1349
BisI GCNGC 6 cut(s) 558, 957, 1201, 1225, 1431, 1434
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 6 cut(s) 559, 958, 1202, 1226, 1432, 1435
BmcAI AGTACT 1 cut(s) 454
Bme1390I CCNGG 3 cut(s) 11, 658, 1716
Bme18I GGWCC 1 cut(s) 697
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 1 cut(s) 481
BmrFI CCNGG 3 cut(s) 11, 658, 1716
BmrI ACTGGG 2 cut(s) 843, 1008
BmsI GCATC 1 cut(s) 66
BmtI GCTAGC 1 cut(s) 774
BmuI ACTGGG 2 cut(s) 843, 1008
BpiI GAAGAC 2 cut(s) 74, 1119
BplI GAGNNNNNCTC 2 cut(s) 556, 588
BpmI CTGGAG 1 cut(s) 147
Bpu10I CCTNAGC 3 cut(s) 234, 282, 299
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 2 cut(s) 508, 1296
Bsa29I ATCGAT 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 1314
BsaJI CCNNGG 3 cut(s) 85, 224, 1877
BsaWI WCCGGW 1 cut(s) 626
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 944, 974
Bsc4I CCNNNNNNNGG 3 cut(s) 660, 1019, 1390
Bse1I ACTGG 3 cut(s) 838, 1014, 1416
Bse3DI GCAATG 2 cut(s) 633, 1203
BseBI CCWGG 3 cut(s) 11, 658, 1716
BseCI ATCGAT 1 cut(s) 401
BseDI CCNNGG 3 cut(s) 85, 224, 1877
BseGI GGATG 3 cut(s) 99, 1420, 1469
BseLI CCNNNNNNNGG 3 cut(s) 660, 1019, 1390
BseMI GCAATG 2 cut(s) 633, 1203
BseMII CTCAG 7 cut(s) 19, 123, 225, 264, 273, 290, 1236
BseNI ACTGG 3 cut(s) 838, 1014, 1416
BseRI GAGGAG 3 cut(s) 824, 1410, 1696
BseSI GKGCMC 2 cut(s) 482, 818
BseXI GCAGC 6 cut(s) 544, 943, 1187, 1236, 1442, 1445
Bsh1236I CGCG 2 cut(s) 1861, 1879
BshNI GGYRCC 1 cut(s) 479
BshVI ATCGAT 1 cut(s) 401
BsiHKAI GWGCWC 1 cut(s) 1595
BsiSI CCGG 2 cut(s) 369, 627
BslFI GGGAC 1 cut(s) 1718
BslI CCNNNNNNNGG 3 cut(s) 660, 1019, 1390
BsmAI GTCTC 3 cut(s) 737, 1314, 1763
BsmFI GGGAC 1 cut(s) 1718
BsmI GAATGC 1 cut(s) 303
Bso31I GGTCTC 1 cut(s) 1314
Bsp1286I GDGCHC 3 cut(s) 482, 818, 1595
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp68I TCGCGA 1 cut(s) 1861
BspACI CCGC 5 cut(s) 768, 1227, 1541, 1877, 1879
BspCNI CTCAG 7 cut(s) 18, 124, 226, 263, 274, 291, 1237
BspDI ATCGAT 1 cut(s) 401
BspFNI CGCG 2 cut(s) 1861, 1879
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 22
BspOI GCTAGC 1 cut(s) 774
BspPI GGATC 4 cut(s) 211, 269, 506, 925
BspT107I GGYRCC 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 1314
BsrDI GCAATG 2 cut(s) 633, 1203
BsrI ACTGG 3 cut(s) 838, 1014, 1416
BssECI CCNNGG 3 cut(s) 85, 224, 1877
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 3 cut(s) 11, 658, 1716
Bst4CI ACNGT 7 cut(s) 317, 435, 751, 1004, 1055, 1649, 1774
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 374, 772
BstDEI CTNAG 8 cut(s) 5, 132, 234, 250, 263, 282, 299, 1245
BstDSI CCRYGG 1 cut(s) 1877
BstENI CCTNNNNNAGG 1 cut(s) 1388
BstF5I GGATG 3 cut(s) 99, 1420, 1469
BstFNI CGCG 2 cut(s) 1861, 1879
BstHHI GCGC 1 cut(s) 1490
BstMAI GTCTC 3 cut(s) 737, 1314, 1763
BstMWI GCNNNNNNNGC 2 cut(s) 477, 1439
BstNI CCWGG 3 cut(s) 11, 658, 1716
BstNSI RCATGY 1 cut(s) 1304
BstSCI CCNGG 3 cut(s) 9, 656, 1714
BstSLI GKGCMC 2 cut(s) 482, 818
BstUI CGCG 2 cut(s) 1861, 1879
BstV1I GCAGC 6 cut(s) 544, 943, 1187, 1236, 1442, 1445
BstV2I GAAGAC 2 cut(s) 74, 1119
BstX2I RGATCY 2 cut(s) 216, 1349
BstYI RGATCY 2 cut(s) 216, 1349
Bsu15I ATCGAT 1 cut(s) 401
BsuI GTATCC 1 cut(s) 54
BsuTUI ATCGAT 1 cut(s) 401
BtgI CCRYGG 1 cut(s) 1877
BtsCI GGATG 3 cut(s) 99, 1420, 1469
BtsI GCAGTG 1 cut(s) 1550
BtsIMutI CAGTG 2 cut(s) 1550, 1593
BtuMI TCGCGA 1 cut(s) 1861
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 374, 772
CaiI CAGNNNCTG 1 cut(s) 1436
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 1 cut(s) 1490
Cfr13I GGNCC 1 cut(s) 697
Cfr42I CCGCGG 1 cut(s) 1880
ClaI ATCGAT 1 cut(s) 401
CseI GACGC 3 cut(s) 79, 460, 948
Csp6I GTAC 6 cut(s) 333, 453, 747, 1071, 1792, 1836
CviAII CATG 7 cut(s) 554, 716, 1039, 1166, 1188, 1197, 1301
CviQI GTAC 6 cut(s) 333, 453, 747, 1071, 1792, 1836
DdeI CTNAG 8 cut(s) 5, 132, 234, 250, 263, 282, 299, 1245
DraI TTTAAA 2 cut(s) 25, 883
DrdI GACNNNNNNGTC 1 cut(s) 1240
DseDI GACNNNNNNGTC 1 cut(s) 1240
Eco130I CCWWGG 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 1314
Eco47I GGWCC 1 cut(s) 697
Eco57I CTGAAG 3 cut(s) 1458, 1617, 1650
Eco72I CACGTG 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 1388
EcoRII CCWGG 3 cut(s) 9, 656, 1714
EcoT14I CCWWGG 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 7 cut(s) 557, 719, 1042, 1169, 1191, 1200, 1304
FaqI GGGAC 1 cut(s) 1718
FatI CATG 7 cut(s) 553, 715, 1038, 1165, 1187, 1196, 1300
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 2 cut(s) 1282, 1422
Fnu4HI GCNGC 6 cut(s) 558, 957, 1201, 1225, 1431, 1434
FokI GGATG 3 cut(s) 106, 1427, 1456
Fsp4HI GCNGC 6 cut(s) 558, 957, 1201, 1225, 1431, 1434
FspBI CTAG 3 cut(s) 165, 771, 963
GlaI GCGC 1 cut(s) 1489
GluI GCNGC 6 cut(s) 558, 957, 1201, 1225, 1431, 1434
GsuI CTGGAG 1 cut(s) 147
HapII CCGG 2 cut(s) 369, 627
HgaI GACGC 3 cut(s) 79, 460, 948
HhaI GCGC 1 cut(s) 1490
Hin1II CATG 7 cut(s) 557, 719, 1042, 1169, 1191, 1200, 1304
Hin6I GCGC 1 cut(s) 1488
HinP1I GCGC 1 cut(s) 1488
HindIII AAGCTT 2 cut(s) 290, 946
HpaII CCGG 2 cut(s) 369, 627
HphI GGTGA 2 cut(s) 298, 429
Hpy166II GTNNAC 4 cut(s) 1071, 1193, 1535, 1836
Hpy8I GTNNAC 4 cut(s) 1071, 1193, 1535, 1836
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 4 cut(s) 185, 450, 1635, 1668
HpyCH4III ACNGT 7 cut(s) 317, 435, 751, 1004, 1055, 1649, 1774
HpyCH4IV ACGT 2 cut(s) 381, 1855
HpyCH4V TGCA 5 cut(s) 79, 198, 924, 1203, 1240
HpyF10VI GCNNNNNNNGC 2 cut(s) 477, 1439
HpyF3I CTNAG 8 cut(s) 5, 132, 234, 250, 263, 282, 299, 1245
HpySE526I ACGT 2 cut(s) 381, 1855
Hsp92II CATG 7 cut(s) 557, 719, 1042, 1169, 1191, 1200, 1304
HspAI GCGC 1 cut(s) 1488
Ksp22I TGATCA 2 cut(s) 1282, 1422
KspI CCGCGG 1 cut(s) 1880
LmnI GCTCC 5 cut(s) 128, 463, 664, 1342, 1590
Lsp1109I GCAGC 6 cut(s) 544, 943, 1187, 1236, 1442, 1445
LweI GCATC 1 cut(s) 66
MaeI CTAG 3 cut(s) 165, 771, 963
MaeII ACGT 2 cut(s) 381, 1855
MaeIII GTNAC 5 cut(s) 435, 566, 777, 1007, 1566
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 2 cut(s) 216, 1349
MhlI GDGCHC 3 cut(s) 482, 818, 1595
MlyI GAGTC 3 cut(s) 143, 223, 1857
MmeI TCCRAC 1 cut(s) 1429
MroXI GAANNNNTTC 2 cut(s) 599, 1063
MslI CAYNNNNRTG 2 cut(s) 579, 1005
MspA1I CMGCKG 5 cut(s) 281, 1227, 1406, 1436, 1879
MspI CCGG 2 cut(s) 369, 627
MspR9I CCNGG 3 cut(s) 11, 658, 1716
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 3 cut(s) 11, 658, 1716
MvnI CGCG 2 cut(s) 1861, 1879
MwoI GCNNNNNNNGC 2 cut(s) 477, 1439
NdeI CATATG 1 cut(s) 679
NheI GCTAGC 1 cut(s) 770
NlaIII CATG 7 cut(s) 557, 719, 1042, 1169, 1191, 1200, 1304
NlaIV GGNNCC 1 cut(s) 481
NmeAIII GCCGAG 2 cut(s) 249, 586
NmuCI GTSAC 3 cut(s) 435, 777, 1007
NruI TCGCGA 1 cut(s) 1861
NspI RCATGY 1 cut(s) 1304
OliI CACNNNNGTG 1 cut(s) 1005
PagI TCATGA 1 cut(s) 1038
PciI ACATGT 1 cut(s) 1300
PctI GAATGC 1 cut(s) 303
PdmI GAANNNNTTC 2 cut(s) 599, 1063
PfeI GAWTC 8 cut(s) 139, 398, 595, 722, 1217, 1400, 1452, 1556
PkrI GCNGC 6 cut(s) 559, 958, 1202, 1226, 1432, 1435
PleI GAGTC 3 cut(s) 142, 223, 1857
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 3 cut(s) 142, 223, 1857
Ppu21I YACGTR 1 cut(s) 382
PscI ACATGT 1 cut(s) 1300
Psp1406I AACGTT 1 cut(s) 1855
Psp6I CCWGG 3 cut(s) 9, 656, 1714
PspCI CACGTG 1 cut(s) 382
PspGI CCWGG 3 cut(s) 9, 656, 1714
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 1 cut(s) 697
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 2 cut(s) 216, 1349
PvuII CAGCTG 3 cut(s) 281, 1406, 1436
RruI TCGCGA 1 cut(s) 1861
RsaI GTAC 6 cut(s) 334, 454, 748, 1072, 1793, 1837
RsaNI GTAC 6 cut(s) 333, 453, 747, 1071, 1792, 1836
RseI CAYNNNNRTG 2 cut(s) 579, 1005
SacII CCGCGG 1 cut(s) 1880
SatI GCNGC 6 cut(s) 558, 957, 1201, 1225, 1431, 1434
Sau96I GGNCC 1 cut(s) 697
ScaI AGTACT 1 cut(s) 454
SchI GAGTC 3 cut(s) 143, 223, 1857
ScrFI CCNGG 3 cut(s) 11, 658, 1716
SduI GDGCHC 3 cut(s) 482, 818, 1595
SfaNI GCATC 1 cut(s) 66
Sfr303I CCGCGG 1 cut(s) 1880
SgrBI CCGCGG 1 cut(s) 1880
SinI GGWCC 1 cut(s) 697
SmiMI CAYNNNNRTG 2 cut(s) 579, 1005
SmlI CTYRAG 2 cut(s) 523, 1311
SmoI CTYRAG 2 cut(s) 523, 1311
SsiI CCGC 5 cut(s) 768, 1227, 1541, 1877, 1879
SspMI CTAG 3 cut(s) 165, 771, 963
StyD4I CCNGG 3 cut(s) 9, 656, 1714
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 7 cut(s) 317, 435, 751, 1004, 1055, 1649, 1774
TaiI ACGT 2 cut(s) 384, 1858
TaqI TCGA 4 cut(s) 137, 401, 1057, 1156
TatI WGTACW 4 cut(s) 452, 1070, 1791, 1835
TfiI GAWTC 8 cut(s) 139, 398, 595, 722, 1217, 1400, 1452, 1556
TscAI CASTG 2 cut(s) 1550, 1600
TseFI GTSAC 3 cut(s) 435, 777, 1007
TseI GCWGC 6 cut(s) 557, 956, 1200, 1224, 1430, 1433
Tsp45I GTSAC 3 cut(s) 435, 777, 1007
TspGWI ACGGA 3 cut(s) 804, 1267, 1736
TspRI CASTG 2 cut(s) 1550, 1600
VpaK11BI GGWCC 1 cut(s) 697
XagI CCTNNNNNAGG 1 cut(s) 1388
XapI RAATTY 4 cut(s) 708, 901, 1655, 1891
XbaI TCTAGA 1 cut(s) 962
XceI RCATGY 1 cut(s) 1304
XmnI GAANNNNTTC 2 cut(s) 599, 1063
XspI CTAG 3 cut(s) 165, 771, 963
ZrmI AGTACT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.