MD06G1062900.v1.1

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006281
Physical Location & Seq
Reverse (-)
10774008 .. 10779407
5400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1062900.v1.1.491

Sequence Viewer

Length: 1941 bp
ATGCGAGCATACACTAGTTCTCTAGACGATGTTGTGAGGAAGACTATGCTCAGCCAGGAGATTATGTTTAAAAAACAGGTTCATGAACTCCATCATCTATATAGGATACAGAAGACACTGATGCAGAACCTTGGCAGGATGGAGTTTGGTAGATACAATCATAGCATAGCTGGTGCTGACTTCAATCTTTTACCTCGGAGAAATTATGGAAGAGACGAACATATGGACTTGTTAGAACGGTGCAAGGGTGTAGATGATAAGATTCAGCCGAGGAATCCTGAGCTTAAACTTCACACAGATCAATGCATTAGCTATATCGATCCAGCGGAGCTGAAGCTTTCCCTGAGCATTCAAGAGGACAATAGGAAAGAGGGAGGTACTAAGAGGATTTGGTTTGATTTAGAAACTCATTCCTGCTCGCAACACATGATTGATTTAGAAGAACCGATTGAAAGGACATCAAATGAGGGCTTGGAACAGTCACCTTCCTTCAGCAGTACTGCTCCAATAACAAACGCCAGGGGCACAAACAATTCAGAAGTTTCTGTCCTCTTTGATCCGATCATCTCGAGTAGTGTGAAGAAAAATATATTCTTTCATGCTGCTGATTGTAACCCTCATTTAGATGGCAGTGAGTGGAATCCTTTCAATCAAGGATTAGAAAAGCGTTCTGGTGGCATTGCAATTAACAATTCATCCACCACAAGGCAGCATTTCGGTTCATATGAAACAGGGCTACTGGACCTTAACAAAATTCAGAATGATGATTCATCTTGTCTCTCAAATGATCATACAGTAGCCCATTTTTCAACAGCTAGCTCTTCACATTTACGCGGATTAGTTAGCAAGGTAGAGGAGGGCACTCATTGTTACGAAACTGGGAAAAAACTAAATAATAATGGCTCAATTGAAACTTTGGAAAGGTTTCAAAAATACAATGCTGGAAACGTCAAAGTTGATGGATCAAGCATAAGTGGAATGGGCAATGTAAGCTTGGAGGCTGCGCCTAGATCGAAGATAGACCTCCAGGAAGCAGTTGGTTTCCACAGTAATGACTCCAGTAATGGAAACAACGGGTTTGTCATAAAGCTCCTAAACAGTCGAAGTAGTTCGTGCACTTCTGCTAGACAAGTAAATTGTGAGAACAACAAAGTAGAAGACCCTGTATTTTCATATACTGATCATTGTCGAAAAGTTGATCCAGATGGACATGGCAATATTTCTTCTGCTTCATGTAAACCATGCTGCATTGGGGAAGATGATTCCAGAAGCGTAAAGACTATGCAGTCTGAAATTGAATATGGAACCTCAAATCCCTTTTCCGTTGATCAGTTTTCAGGAACACATGTAGGGTCTCAAGTTTCAGAAACCTTAATGGGTGAGCAGGACCGAAGATCTTCTGACAACAGTGAATTAAAACACAAAGACCTTGACAAGGGAGGGGATTCAGCTGAACTGAATGTTTTGATCAGAGCAGCTGAAGCACTTGTCAGAATCTCATTGGAGAGTTCATCATGTTGTCATGATTGCACTTACGAAGTAGGAGGTGCAAATAAGATGGAAACGAAGGGGAGTGAACAACCGCAGTGTTCTTCTGATTCTTATGAGTTACTCACACTAAATCTAATAGAGAGCACTGAAGATGACTATTGCGTGTCATTAAAGCCTTCTGAGGTGAATTACAATGACAGTAAGGAATTTGGGGTTAAGTTGAGAAGAGGGAGGAGAATGAAAGATTTTCAGAGGGACATTCTTCCAGGTCTGGCATCTCTTTCCAGACACGAAATTCGTGAAGATATTAACATTATGGAGACAGTTTTAAGATCAAGAGAGTACAAAAAGCTAAGAGGCAAGGTGGCTGATTATCAAAGCTGGTGTACTCCTGTCAAAAACAAACGTTCGCGACTCAACTATGTTCCGCGGAGGAAAAGAAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

647

Amino Acids

72.5

Weight (kDa)

6.15

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 527 - 608 4.7e-18 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1285
AccII CGCG 3 cut(s) 834, 1903, 1921
AciI CCGC 5 cut(s) 326, 834, 1583, 1919, 1921
AclI AACGTT 1 cut(s) 1897
AclWI GGATC 4 cut(s) 314, 551, 970, 1193
AcsI RAATTY 4 cut(s) 753, 1697, 1785, 1933
AcuI CTGAAG 4 cut(s) 353, 475, 1500, 1659
AfaI GTAC 4 cut(s) 379, 499, 1835, 1879
AfiI CCNNNNNNNGG 2 cut(s) 705, 1435
AflIII ACRYGT 1 cut(s) 1345
AgsI TTSAA 8 cut(s) 184, 353, 452, 649, 810, 911, 929, 1298
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 4 cut(s) 54, 518, 1026, 1756
AloI GAACNNNNNNTCC 2 cut(s) 1297, 1329
Alw21I GWGCWC 2 cut(s) 1118, 1637
Alw26I GTCTC 4 cut(s) 207, 782, 1359, 1805
Alw44I GTGCAC 1 cut(s) 1114
AlwI GGATC 4 cut(s) 314, 551, 970, 1193
Ama87I CYCGRG 1 cut(s) 568
ApaLI GTGCAC 1 cut(s) 1114
ApeKI GCWGC 5 cut(s) 602, 709, 1001, 1245, 1475
ApoI RAATTY 4 cut(s) 753, 1697, 1785, 1933
ArsI GACNNNNNNTTYG 2 cut(s) 1770, 1802
Asp700I GAANNNNTTC 4 cut(s) 644, 924, 1108, 1396
AspLEI GCGC 1 cut(s) 1006
AspS9I GGNCC 2 cut(s) 742, 1387
AsuHPI GGTGA 3 cut(s) 474, 1391, 1687
AsuNHI GCTAGC 1 cut(s) 815
AvaI CYCGRG 1 cut(s) 568
AvaII GGWCC 2 cut(s) 742, 1387
BaeGI GKGCMC 3 cut(s) 527, 863, 1118
BbsI GAAGAC 3 cut(s) 47, 119, 1164
Bbv12I GWGCWC 2 cut(s) 1118, 1637
BbvI GCAGC 5 cut(s) 589, 721, 988, 1232, 1487
BccI CCATC 6 cut(s) 99, 133, 620, 953, 1199, 1552
BciT130I CCWGG 4 cut(s) 56, 520, 1028, 1758
BciVI GTATCC 1 cut(s) 99
BclI TGATCA 4 cut(s) 787, 1180, 1327, 1467
BcoDI GTCTC 4 cut(s) 207, 782, 1359, 1805
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 5 cut(s) 15, 23, 816, 1008, 1125
BfuI GTATCC 1 cut(s) 99
BglII AGATCT 1 cut(s) 1394
BisI GCNGC 5 cut(s) 603, 710, 1002, 1246, 1476
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 5 cut(s) 604, 711, 1003, 1247, 1477
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 4 cut(s) 56, 520, 1028, 1758
Bme18I GGWCC 2 cut(s) 742, 1387
BmeT110I CYCGRG 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 742, 1387
BmiI GGNNCC 1 cut(s) 1306
BmrFI CCNGG 4 cut(s) 56, 520, 1028, 1758
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 2 cut(s) 111, 1775
BmtI GCTAGC 1 cut(s) 819
BmuI ACTGGG 1 cut(s) 888
BpiI GAAGAC 3 cut(s) 47, 119, 1164
BpmI CTGGAG 2 cut(s) 1010, 1042
Bpu10I CCTNAGC 2 cut(s) 279, 344
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 1341
Bsa29I ATCGAT 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 1359
BsaJI CCNNGG 5 cut(s) 130, 194, 269, 519, 1919
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 2 cut(s) 705, 1435
Bse1I ACTGG 3 cut(s) 744, 883, 1059
Bse3DI GCAATG 2 cut(s) 678, 991
BseBI CCWGG 4 cut(s) 56, 520, 1028, 1758
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 5 cut(s) 130, 194, 269, 519, 1919
BseGI GGATG 2 cut(s) 144, 695
BseLI CCNNNNNNNGG 2 cut(s) 705, 1435
BseMI GCAATG 2 cut(s) 678, 991
BseMII CTCAG 4 cut(s) 64, 270, 335, 1662
BseNI ACTGG 3 cut(s) 744, 883, 1059
BseRI GAGGAG 2 cut(s) 869, 1738
BseSI GKGCMC 3 cut(s) 527, 863, 1118
BseXI GCAGC 5 cut(s) 589, 721, 988, 1232, 1487
Bsh1236I CGCG 3 cut(s) 834, 1903, 1921
BshVI ATCGAT 1 cut(s) 318
BsiHKAI GWGCWC 2 cut(s) 1118, 1637
BsiHKCI CYCGRG 1 cut(s) 568
BslFI GGGAC 1 cut(s) 1760
BslI CCNNNNNNNGG 2 cut(s) 705, 1435
BsmAI GTCTC 4 cut(s) 207, 782, 1359, 1805
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 1760
BsmI GAATGC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 1359
BsoBI CYCGRG 1 cut(s) 568
Bsp1286I GDGCHC 4 cut(s) 527, 863, 1118, 1637
Bsp1720I GCTNAGC 1 cut(s) 50
Bsp68I TCGCGA 1 cut(s) 1903
BspACI CCGC 5 cut(s) 326, 834, 1583, 1919, 1921
BspCNI CTCAG 4 cut(s) 63, 271, 336, 1663
BspDI ATCGAT 1 cut(s) 318
BspFNI CGCG 3 cut(s) 834, 1903, 1921
BspHI TCATGA 2 cut(s) 82, 1522
BspLI GGNNCC 1 cut(s) 1306
BspOI GCTAGC 1 cut(s) 819
BspPI GGATC 4 cut(s) 314, 551, 970, 1193
BspQI GCTCTTC 1 cut(s) 826
BspTNI GGTCTC 1 cut(s) 1359
BsrDI GCAATG 2 cut(s) 678, 991
BsrI ACTGG 3 cut(s) 744, 883, 1059
BssECI CCNNGG 5 cut(s) 130, 194, 269, 519, 1919
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 4 cut(s) 56, 520, 1028, 1758
Bst4CI ACNGT 8 cut(s) 240, 480, 796, 1049, 1100, 1409, 1691, 1816
Bst6I CTCTTC 3 cut(s) 205, 826, 1711
BstC8I GCNNGC 3 cut(s) 6, 419, 817
BstDEI CTNAG 6 cut(s) 50, 279, 344, 381, 1671, 1844
BstDSI CCRYGG 1 cut(s) 1919
BstENI CCTNNNNNAGG 1 cut(s) 1433
BstF5I GGATG 2 cut(s) 144, 695
BstFNI CGCG 3 cut(s) 834, 1903, 1921
BstHHI GCGC 1 cut(s) 1006
BstMAI GTCTC 4 cut(s) 207, 782, 1359, 1805
BstMWI GCNNNNNNNGC 2 cut(s) 990, 1481
BstNI CCWGG 4 cut(s) 56, 520, 1028, 1758
BstNSI RCATGY 1 cut(s) 1349
BstSCI CCNGG 4 cut(s) 54, 518, 1026, 1756
BstSLI GKGCMC 3 cut(s) 527, 863, 1118
BstUI CGCG 3 cut(s) 834, 1903, 1921
BstV1I GCAGC 5 cut(s) 589, 721, 988, 1232, 1487
BstV2I GAAGAC 3 cut(s) 47, 119, 1164
BstX2I RGATCY 1 cut(s) 1394
BstYI RGATCY 1 cut(s) 1394
Bsu15I ATCGAT 1 cut(s) 318
BsuI GTATCC 1 cut(s) 99
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 1919
BtsCI GGATG 2 cut(s) 144, 695
BtsI GCAGTG 2 cut(s) 637, 1592
BtsIMutI CAGTG 5 cut(s) 116, 637, 1414, 1592, 1635
BtuMI TCGCGA 1 cut(s) 1903
Cac8I GCNNGC 3 cut(s) 6, 419, 817
CciI TCATGA 2 cut(s) 82, 1522
CfoI GCGC 1 cut(s) 1006
Cfr13I GGNCC 2 cut(s) 742, 1387
Cfr42I CCGCGG 1 cut(s) 1922
ClaI ATCGAT 1 cut(s) 318
Csp6I GTAC 4 cut(s) 378, 498, 1834, 1878
CviAII CATG 9 cut(s) 83, 427, 599, 1211, 1233, 1242, 1346, 1515, 1523
CviQI GTAC 4 cut(s) 378, 498, 1834, 1878
DdeI CTNAG 6 cut(s) 50, 279, 344, 381, 1671, 1844
DraI TTTAAA 1 cut(s) 70
DrdI GACNNNNNNGTC 1 cut(s) 1285
DseDI GACNNNNNNGTC 1 cut(s) 1285
Eam1104I CTCTTC 3 cut(s) 205, 826, 1711
EarI CTCTTC 3 cut(s) 205, 826, 1711
Eco130I CCWWGG 1 cut(s) 130
Eco31I GGTCTC 1 cut(s) 1359
Eco47I GGWCC 2 cut(s) 742, 1387
Eco57I CTGAAG 4 cut(s) 353, 475, 1500, 1659
Eco88I CYCGRG 1 cut(s) 568
EcoNI CCTNNNNNAGG 1 cut(s) 1433
EcoRII CCWGG 4 cut(s) 54, 518, 1026, 1756
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 9 cut(s) 86, 430, 602, 1214, 1236, 1245, 1349, 1518, 1526
FaqI GGGAC 1 cut(s) 1760
FatI CATG 9 cut(s) 82, 426, 598, 1210, 1232, 1241, 1345, 1514, 1522
FauNDI CATATG 2 cut(s) 222, 724
FbaI TGATCA 4 cut(s) 787, 1180, 1327, 1467
Fnu4HI GCNGC 5 cut(s) 603, 710, 1002, 1246, 1476
FokI GGATG 2 cut(s) 151, 682
Fsp4HI GCNGC 5 cut(s) 603, 710, 1002, 1246, 1476
FspBI CTAG 5 cut(s) 15, 23, 816, 1008, 1125
GlaI GCGC 1 cut(s) 1005
GluI GCNGC 5 cut(s) 603, 710, 1002, 1246, 1476
GsuI CTGGAG 2 cut(s) 1010, 1042
HhaI GCGC 1 cut(s) 1006
Hin1II CATG 9 cut(s) 86, 430, 602, 1214, 1236, 1245, 1349, 1518, 1526
Hin6I GCGC 1 cut(s) 1004
HinP1I GCGC 1 cut(s) 1004
HindIII AAGCTT 2 cut(s) 335, 991
HphI GGTGA 3 cut(s) 474, 1391, 1687
Hpy166II GTNNAC 4 cut(s) 1116, 1238, 1577, 1878
Hpy8I GTNNAC 4 cut(s) 1116, 1238, 1577, 1878
HpyAV CCTTC 4 cut(s) 495, 499, 1561, 1677
HpyCH4III ACNGT 8 cut(s) 240, 480, 796, 1049, 1100, 1409, 1691, 1816
HpyCH4IV ACGT 2 cut(s) 948, 1897
HpyCH4V TGCA 9 cut(s) 124, 243, 306, 683, 1116, 1248, 1285, 1530, 1550
HpyF10VI GCNNNNNNNGC 2 cut(s) 990, 1481
HpyF3I CTNAG 6 cut(s) 50, 279, 344, 381, 1671, 1844
HpySE526I ACGT 2 cut(s) 948, 1897
Hsp92II CATG 9 cut(s) 86, 430, 602, 1214, 1236, 1245, 1349, 1518, 1526
HspAI GCGC 1 cut(s) 1004
Ksp22I TGATCA 4 cut(s) 787, 1180, 1327, 1467
KspI CCGCGG 1 cut(s) 1922
LguI GCTCTTC 1 cut(s) 826
LmnI GCTCC 3 cut(s) 328, 508, 1095
Lsp1109I GCAGC 5 cut(s) 589, 721, 988, 1232, 1487
LweI GCATC 2 cut(s) 111, 1775
MaeI CTAG 5 cut(s) 15, 23, 816, 1008, 1125
MaeII ACGT 2 cut(s) 948, 1897
MaeIII GTNAC 4 cut(s) 480, 611, 869, 1608
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 1 cut(s) 1394
MhlI GDGCHC 4 cut(s) 527, 863, 1118, 1637
MlyI GAGTC 2 cut(s) 1049, 1899
Mph1103I ATGCAT 1 cut(s) 308
MroXI GAANNNNTTC 4 cut(s) 644, 924, 1108, 1396
MslI CAYNNNNRTG 2 cut(s) 624, 1050
MspA1I CMGCKG 4 cut(s) 326, 1451, 1478, 1921
MspR9I CCNGG 4 cut(s) 56, 520, 1028, 1758
MunI CAATTG 1 cut(s) 906
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 4 cut(s) 56, 520, 1028, 1758
MvnI CGCG 3 cut(s) 834, 1903, 1921
MwoI GCNNNNNNNGC 2 cut(s) 990, 1481
NdeI CATATG 2 cut(s) 222, 724
NheI GCTAGC 1 cut(s) 815
NlaIII CATG 9 cut(s) 86, 430, 602, 1214, 1236, 1245, 1349, 1518, 1526
NlaIV GGNNCC 1 cut(s) 1306
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 480
NruI TCGCGA 1 cut(s) 1903
NsiI ATGCAT 1 cut(s) 308
NspI RCATGY 1 cut(s) 1349
PaeR7I CTCGAG 1 cut(s) 568
PagI TCATGA 2 cut(s) 82, 1522
PciI ACATGT 1 cut(s) 1345
PciSI GCTCTTC 1 cut(s) 826
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 4 cut(s) 644, 924, 1108, 1396
PfeI GAWTC 8 cut(s) 262, 274, 640, 767, 1262, 1445, 1494, 1598
PfoI TCCNGGA 1 cut(s) 1026
PkrI GCNGC 5 cut(s) 604, 711, 1003, 1247, 1477
PleI GAGTC 2 cut(s) 1049, 1899
PpsI GAGTC 2 cut(s) 1049, 1899
PscI ACATGT 1 cut(s) 1345
Psp1406I AACGTT 1 cut(s) 1897
Psp6I CCWGG 4 cut(s) 54, 518, 1026, 1756
PspGI CCWGG 4 cut(s) 54, 518, 1026, 1756
PspN4I GGNNCC 1 cut(s) 1306
PspPI GGNCC 2 cut(s) 742, 1387
PsuI RGATCY 1 cut(s) 1394
PvuII CAGCTG 2 cut(s) 1451, 1478
RruI TCGCGA 1 cut(s) 1903
RsaI GTAC 4 cut(s) 379, 499, 1835, 1879
RsaNI GTAC 4 cut(s) 378, 498, 1834, 1878
RseI CAYNNNNRTG 2 cut(s) 624, 1050
SacII CCGCGG 1 cut(s) 1922
SapI GCTCTTC 1 cut(s) 826
SatI GCNGC 5 cut(s) 603, 710, 1002, 1246, 1476
Sau96I GGNCC 2 cut(s) 742, 1387
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 2 cut(s) 1049, 1899
ScrFI CCNGG 4 cut(s) 56, 520, 1028, 1758
SduI GDGCHC 4 cut(s) 527, 863, 1118, 1637
SfaNI GCATC 2 cut(s) 111, 1775
Sfr274I CTCGAG 1 cut(s) 568
Sfr303I CCGCGG 1 cut(s) 1922
SgrBI CCGCGG 1 cut(s) 1922
SinI GGWCC 2 cut(s) 742, 1387
SlaI CTCGAG 1 cut(s) 568
SmiMI CAYNNNNRTG 2 cut(s) 624, 1050
SmlI CTYRAG 2 cut(s) 568, 1356
SmoI CTYRAG 2 cut(s) 568, 1356
SpeI ACTAGT 1 cut(s) 14
SsiI CCGC 5 cut(s) 326, 834, 1583, 1919, 1921
SspI AATATT 1 cut(s) 1219
SspMI CTAG 5 cut(s) 15, 23, 816, 1008, 1125
StyD4I CCNGG 4 cut(s) 54, 518, 1026, 1756
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 8 cut(s) 240, 480, 796, 1049, 1100, 1409, 1691, 1816
TaiI ACGT 2 cut(s) 951, 1900
TaqI TCGA 5 cut(s) 318, 569, 1013, 1102, 1189
TaqII GACCGA 1 cut(s) 1404
TatI WGTACW 3 cut(s) 497, 1833, 1877
TfiI GAWTC 8 cut(s) 262, 274, 640, 767, 1262, 1445, 1494, 1598
TscAI CASTG 5 cut(s) 123, 637, 1414, 1592, 1642
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 5 cut(s) 602, 709, 1001, 1245, 1475
Tsp45I GTSAC 1 cut(s) 480
TspGWI ACGGA 1 cut(s) 1312
TspRI CASTG 5 cut(s) 123, 637, 1414, 1592, 1642
VneI GTGCAC 1 cut(s) 1114
VpaK11BI GGWCC 2 cut(s) 742, 1387
XagI CCTNNNNNAGG 1 cut(s) 1433
XapI RAATTY 4 cut(s) 753, 1697, 1785, 1933
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 1 cut(s) 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 1034
XhoI CTCGAG 1 cut(s) 568
XmnI GAANNNNTTC 4 cut(s) 644, 924, 1108, 1396
XspI CTAG 5 cut(s) 15, 23, 816, 1008, 1125
ZrmI AGTACT 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.