RLG00000003583

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006281
Physical Location & Seq
Reverse (-)
50297978 .. 50300697
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003583

Sequence Viewer

Length: 1827 bp
ATGGGCCAAAGAAAAATGTTGATGCGAGGAGATTTTGATCTGAATTCGATGCAACCGTATACTGGTTCTCTGAACGATGTCTTGAGGAACACTATGCTCAACCAGGAGCTGGTATTTAAAGGCCAGGTTCAAGAACTCCACCATCTATATAGGACACAGAAGATGCTGATGCAGAATCTCGACGGAAAAGGGTATCATAGATACAATGTCAGCAAGGCGAGTGCTCAGTCAAATCTGTCACATTGGACAAATTCTATGATAGATGAACCAAGGGACTTGTTTGAAGGACACAAGAGTACATGTGATAAGATTCAGCCAAGGCCTCTTGGTCTTAAGCATCCCTTAGATCCGCTCAACAAACATATTGATCCAGCGGAGCTGAAGCTTTCCCTGAGAATTCAAGAGGACAGCAGGAGGGAGGGAAGCACGAAGAGAAATTGGTTTGATGTAGGATCTCATTCTTCCTCACCCATTGTCATTGATTTAGAGGAAGATGAAAGTGACTGGAATCCTTTCAATAAAGGATTGAATAGGTGTTCTGGTGGTATCTCAATTAACAAGTTATCCACCAGGAGCCAACAGTTCAGTTCATATGAAGCAGGGCTTCTGGACCTTAATAAAACCCAGCCCGATGATTCATCTTGTCTCTCCAATGATCCTACGGTAGTCCATCCTTCAACAGCCAGCTCATCGCATGCTTTCCGTGGGTTAGCTGGCAAGATAAAAGAGGGCACTGTTTGTTTCACTGCTGAGAAAAAGGAACATGGTGGAAATTTTTCTTCCTTGAATTCCACCAGGGAAAATGAGAAAACAGAATACCTGGGCAGGAGTTTGAAAGTTGATGGATTTAGAGCAAGTGAAATGGACAGTGCAAGTTTGGAGGCAGCTGTGTCTAGATCCAAATCAGACGTTTGTGAAGCAGTTGGCTGCAACAGCAATGACCCCAAAAATGGAAGTGATGGGTTCATCCTGAAACTTCTAAACAGTCGAACTCATTCATGTGGAGCTTCTAAACAAGCAAACTTGGATAACACCAAAGTAGAAGACCCTGTATTTCCATATTCTGGTCATAGTAAAAAAACAGATCAAGATGGACATGGCAATATATCTTCTTCTGCAAGTAAGCCGTGCTGCATTGGTGATAATGATTCCAGTAGTGTGAAGACCATACTATCTGAGGTTGAGTTCGGAAACTCAAACCCTTTTTCTTCTGATCAGTTTTCAGCAACACATGTAGGATCTCAAGTTTCGGAAACTTTATTGGGTGAGCAGGACCAAAGATCTTCCGACAGCAGTGAATCTAAACACAAATGCGTCAACAGGGAAGAAGAATCAGCTGAAGTGGATGCTTTGACCAGCGAGGCAGCTGAAGCACTTCTTGGAATGTCATTGGAGAGCTCCTTTTGTTATCAAGATTCTGCGGGCAAAGTGGGAGAATCAAATAAGGTCGAAAAGGAGAGTGAGAAACCACAGTATTCTTGTGATTTTTTTGAGTTAATGACACTAACCCTTATGGAGAGCAGTGTGGATGACTATTCTGTGTCATCAAATCCTTCTGAAGTAAGTTTCACAGAAAATAAGGATGTCGGTTTAAAGTTAAGAAGGGGAAGGAGATTGAAAGATTTTCAGAAGGACATACTTCCTGGTCTGGCATCTCTTTCCAGACATGAAATTCGCGAAGATATAAACATCATGGAGGGAGTTTTAAGGTCAAGAGAGTACAAAAGATTACAAGCCAAAATGGCTGATGGTCACGGCTGGTGTACTCCTGTCAGAAACAAACGTTCGCGACTGAACCATGTTACGCGGAGGAAAAAATTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

609

Amino Acids

67.61

Weight (kDa)

6.2

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 479 - 571 1.8e-21 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 109, 1064
AccBSI CCGCTC 1 cut(s) 352
AccI GTMKAC 1 cut(s) 59
AccII CGCG 3 cut(s) 1677, 1789, 1807
AciI CCGC 4 cut(s) 350, 374, 1421, 1807
AclI AACGTT 1 cut(s) 1783
AclWI GGATC 6 cut(s) 341, 362, 460, 650, 891, 1246
AcsI RAATTY 7 cut(s) 43, 250, 396, 772, 787, 1671, 1817
AcuI CTGAAG 4 cut(s) 401, 1359, 1389, 1578
AfaI GTAC 3 cut(s) 298, 1721, 1765
AfiI CCNNNNNNNGG 4 cut(s) 62, 109, 950, 1064
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 2 cut(s) 299, 1231
AgsI TTSAA 9 cut(s) 131, 284, 401, 517, 529, 678, 787, 835, 1618
AjnI CCWGG 6 cut(s) 102, 123, 569, 794, 819, 1642
AjuI GAANNNNNNNTTGG 2 cut(s) 1362, 1394
Alw21I GWGCWC 2 cut(s) 226, 1400
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 6 cut(s) 341, 362, 460, 650, 891, 1246
AlwNI CAGNNNCTG 1 cut(s) 109
AoxI GGCC 3 cut(s) 4, 121, 320
ApeKI GCWGC 4 cut(s) 884, 927, 1131, 1364
ApoI RAATTY 7 cut(s) 43, 250, 396, 772, 787, 1671, 1817
ArsI GACNNNNNNTTYG 2 cut(s) 1656, 1688
Asp700I GAANNNNTTC 5 cut(s) 512, 775, 994, 1374, 1817
AspS9I GGNCC 3 cut(s) 4, 610, 1273
AsuHPI GGTGA 3 cut(s) 459, 1151, 1277
AvaII GGWCC 2 cut(s) 610, 1273
BaeGI GKGCMC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 1400
BbsI GAAGAC 2 cut(s) 1050, 1169
Bbv12I GWGCWC 2 cut(s) 226, 1400
BbvI GCAGC 4 cut(s) 896, 914, 1118, 1376
BccI CCATC 6 cut(s) 150, 678, 836, 953, 1085, 1742
BceAI ACGGC 2 cut(s) 1111, 1771
BciT130I CCWGG 6 cut(s) 104, 125, 571, 796, 821, 1644
BclI TGATCA 1 cut(s) 1213
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 1 cut(s) 894
BfrI CTTAAG 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 1742
BglII AGATCT 1 cut(s) 1280
BisI GCNGC 4 cut(s) 885, 928, 1132, 1365
BlsI GCNGC 4 cut(s) 886, 929, 1133, 1366
Bme1390I CCNGG 6 cut(s) 104, 125, 571, 796, 821, 1644
Bme18I GGWCC 2 cut(s) 610, 1273
BmgT120I GGNCC 3 cut(s) 4, 610, 1273
BmiI GGNNCC 1 cut(s) 575
BmrFI CCNGG 6 cut(s) 104, 125, 571, 796, 821, 1644
BmsI GCATC 7 cut(s) 12, 39, 153, 159, 346, 1336, 1661
BpiI GAAGAC 2 cut(s) 1050, 1169
BpuEI CTTGAG 2 cut(s) 103, 1227
BsaBI GATNNNNATC 2 cut(s) 36, 901
BsaJI CCNNGG 5 cut(s) 269, 317, 703, 795, 820
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 109, 950, 1064
Bse1I ACTGG 3 cut(s) 67, 509, 1152
Bse3DI GCAATG 1 cut(s) 943
Bse8I GATNNNNATC 2 cut(s) 36, 901
BseBI CCWGG 6 cut(s) 104, 125, 571, 796, 821, 1644
BseDI CCNNGG 5 cut(s) 269, 317, 703, 795, 820
BseGI GGATG 6 cut(s) 337, 670, 966, 1351, 1534, 1588
BseJI GATNNNNATC 2 cut(s) 36, 901
BseLI CCNNNNNNNGG 4 cut(s) 62, 109, 950, 1064
BseMI GCAATG 1 cut(s) 943
BseMII CTCAG 4 cut(s) 239, 383, 741, 1167
BseNI ACTGG 3 cut(s) 67, 509, 1152
BseRI GAGGAG 1 cut(s) 42
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 4 cut(s) 896, 914, 1118, 1376
BseYI CCCAGC 1 cut(s) 624
Bsh1236I CGCG 3 cut(s) 1677, 1789, 1807
BshFI GGCC 3 cut(s) 6, 123, 322
BsiHKAI GWGCWC 2 cut(s) 226, 1400
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 4 cut(s) 62, 109, 950, 1064
BsmAI GTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 3 cut(s) 6, 123, 322
Bsp1286I GDGCHC 3 cut(s) 226, 734, 1400
Bsp68I TCGCGA 2 cut(s) 1677, 1789
BspACI CCGC 4 cut(s) 350, 374, 1421, 1807
BspANI GGCC 3 cut(s) 6, 123, 322
BspCNI CTCAG 4 cut(s) 238, 384, 742, 1168
BspFNI CGCG 3 cut(s) 1677, 1789, 1807
BspLI GGNNCC 1 cut(s) 575
BspPI GGATC 6 cut(s) 341, 362, 460, 650, 891, 1246
BspTI CTTAAG 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 943
BsrI ACTGG 3 cut(s) 67, 509, 1152
BssECI CCNNGG 5 cut(s) 269, 317, 703, 795, 820
BssNAI GTATAC 1 cut(s) 60
BssT1I CCWWGG 2 cut(s) 269, 317
Bst1107I GTATAC 1 cut(s) 60
Bst2UI CCWGG 6 cut(s) 104, 125, 571, 796, 821, 1644
Bst4CI ACNGT 7 cut(s) 57, 582, 664, 736, 869, 986, 1473
Bst6I CTCTTC 1 cut(s) 425
BstAFI CTTAAG 1 cut(s) 332
BstC8I GCNNGC 4 cut(s) 685, 696, 715, 1423
BstDEI CTNAG 5 cut(s) 225, 343, 392, 750, 1176
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 6 cut(s) 337, 670, 966, 1351, 1534, 1588
BstFNI CGCG 3 cut(s) 1677, 1789, 1807
BstMAI GTCTC 1 cut(s) 650
BstMWI GCNNNNNNNGC 3 cut(s) 933, 1370, 1742
BstNI CCWGG 6 cut(s) 104, 125, 571, 796, 821, 1644
BstNSI RCATGY 3 cut(s) 303, 698, 1235
BstSCI CCNGG 6 cut(s) 102, 123, 569, 794, 819, 1642
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 3 cut(s) 1677, 1789, 1807
BstV1I GCAGC 4 cut(s) 896, 914, 1118, 1376
BstV2I GAAGAC 2 cut(s) 1050, 1169
BstX2I RGATCY 5 cut(s) 346, 452, 896, 1238, 1280
BstYI RGATCY 5 cut(s) 346, 452, 896, 1238, 1280
BstZ17I GTATAC 1 cut(s) 60
BsuRI GGCC 3 cut(s) 6, 123, 322
BtgI CCRYGG 1 cut(s) 703
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 6 cut(s) 337, 670, 966, 1351, 1534, 1588
BtsI GCAGTG 3 cut(s) 744, 1300, 1528
BtsIMutI CAGTG 5 cut(s) 732, 744, 874, 1300, 1528
BtuMI TCGCGA 2 cut(s) 1677, 1789
Cac8I GCNNGC 4 cut(s) 685, 696, 715, 1423
CaiI CAGNNNCTG 1 cut(s) 109
Cfr13I GGNCC 3 cut(s) 4, 610, 1273
CseI GACGC 1 cut(s) 1303
Csp6I GTAC 3 cut(s) 297, 1720, 1764
CviAII CATG 9 cut(s) 300, 695, 764, 999, 1097, 1232, 1667, 1693, 1799
CviQI GTAC 3 cut(s) 297, 1720, 1764
DdeI CTNAG 5 cut(s) 225, 343, 392, 750, 1176
DraI TTTAAA 2 cut(s) 118, 1593
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 1398
Eco130I CCWWGG 2 cut(s) 269, 317
Eco147I AGGCCT 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 1400
Eco47I GGWCC 2 cut(s) 610, 1273
Eco53kI GAGCTC 1 cut(s) 1398
Eco57I CTGAAG 4 cut(s) 401, 1359, 1389, 1578
EcoICRI GAGCTC 1 cut(s) 1398
EcoRI GAATTC 3 cut(s) 43, 396, 787
EcoRII CCWGG 6 cut(s) 102, 123, 569, 794, 819, 1642
EcoT14I CCWWGG 2 cut(s) 269, 317
EcoT38I GRGCYC 1 cut(s) 1400
ErhI CCWWGG 2 cut(s) 269, 317
FaeI CATG 9 cut(s) 303, 698, 767, 1002, 1100, 1235, 1670, 1696, 1802
FalI AAGNNNNNCTT 6 cut(s) 326, 358, 588, 620, 1362, 1394
FaqI GGGAC 1 cut(s) 287
FatI CATG 9 cut(s) 299, 694, 763, 998, 1096, 1231, 1666, 1692, 1798
FauI CCCGC 1 cut(s) 1414
FauNDI CATATG 1 cut(s) 592
FbaI TGATCA 1 cut(s) 1213
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 4 cut(s) 885, 928, 1132, 1365
FokI GGATG 6 cut(s) 324, 657, 953, 1358, 1541, 1595
FriOI GRGCYC 1 cut(s) 1400
Fsp4HI GCNGC 4 cut(s) 885, 928, 1132, 1365
FspBI CTAG 1 cut(s) 894
GluI GCNGC 4 cut(s) 885, 928, 1132, 1365
GsaI CCCAGC 1 cut(s) 628
HaeIII GGCC 3 cut(s) 6, 123, 322
HgaI GACGC 1 cut(s) 1303
Hin1II CATG 9 cut(s) 303, 698, 767, 1002, 1100, 1235, 1670, 1696, 1802
HincII GTYRAC 1 cut(s) 1318
HindII GTYRAC 1 cut(s) 1318
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 9 cut(s) 175, 310, 508, 635, 1148, 1298, 1331, 1415, 1436
HphI GGTGA 3 cut(s) 459, 1151, 1277
Hpy166II GTNNAC 3 cut(s) 60, 1318, 1764
Hpy8I GTNNAC 3 cut(s) 60, 1318, 1764
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 6 cut(s) 278, 684, 1563, 1596, 1602, 1624
HpyCH4III ACNGT 7 cut(s) 57, 582, 664, 736, 869, 986, 1473
HpyCH4IV ACGT 2 cut(s) 909, 1783
HpyCH4V TGCA 6 cut(s) 52, 172, 872, 930, 1118, 1134
HpyF10VI GCNNNNNNNGC 3 cut(s) 933, 1370, 1742
HpyF3I CTNAG 5 cut(s) 225, 343, 392, 750, 1176
HpySE526I ACGT 2 cut(s) 909, 1783
Hsp92II CATG 9 cut(s) 303, 698, 767, 1002, 1100, 1235, 1670, 1696, 1802
Ksp22I TGATCA 1 cut(s) 1213
LmnI GCTCC 5 cut(s) 106, 376, 573, 1004, 1403
Lsp1109I GCAGC 4 cut(s) 896, 914, 1118, 1376
LweI GCATC 7 cut(s) 12, 39, 153, 159, 346, 1336, 1661
MaeI CTAG 1 cut(s) 894
MaeII ACGT 2 cut(s) 909, 1783
MaeIII GTNAC 4 cut(s) 237, 500, 1751, 1801
MbiI CCGCTC 1 cut(s) 352
MflI RGATCY 5 cut(s) 346, 452, 896, 1238, 1280
MhlI GDGCHC 3 cut(s) 226, 734, 1400
MluCI AATT 9 cut(s) 43, 250, 396, 436, 552, 772, 787, 1671, 1817
MmeI TCCRAC 1 cut(s) 1311
MroXI GAANNNNTTC 5 cut(s) 512, 775, 994, 1374, 1817
MseI TTAA 8 cut(s) 117, 333, 555, 615, 1496, 1592, 1598, 1706
MslI CAYNNNNRTG 1 cut(s) 999
MspA1I CMGCKG 4 cut(s) 374, 887, 1337, 1367
MspCI CTTAAG 1 cut(s) 332
MspR9I CCNGG 6 cut(s) 104, 125, 571, 796, 821, 1644
MvaI CCWGG 6 cut(s) 104, 125, 571, 796, 821, 1644
MvnI CGCG 3 cut(s) 1677, 1789, 1807
MwoI GCNNNNNNNGC 3 cut(s) 933, 1370, 1742
NdeI CATATG 1 cut(s) 592
NlaIII CATG 9 cut(s) 303, 698, 767, 1002, 1100, 1235, 1670, 1696, 1802
NlaIV GGNNCC 1 cut(s) 575
NmuCI GTSAC 3 cut(s) 237, 500, 1751
NruI TCGCGA 2 cut(s) 1677, 1789
NspI RCATGY 3 cut(s) 303, 698, 1235
PaeI GCATGC 1 cut(s) 698
PceI AGGCCT 1 cut(s) 322
PciI ACATGT 2 cut(s) 299, 1231
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PdmI GAANNNNTTC 5 cut(s) 512, 775, 994, 1374, 1817
PfeI GAWTC 9 cut(s) 175, 310, 508, 635, 1148, 1298, 1331, 1415, 1436
PflMI CCANNNNNTGG 2 cut(s) 109, 1064
PkrI GCNGC 4 cut(s) 886, 929, 1133, 1366
PscI ACATGT 2 cut(s) 299, 1231
Psp124BI GAGCTC 1 cut(s) 1400
Psp1406I AACGTT 1 cut(s) 1783
Psp6I CCWGG 6 cut(s) 102, 123, 569, 794, 819, 1642
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 6 cut(s) 102, 123, 569, 794, 819, 1642
PspN4I GGNNCC 1 cut(s) 575
PspPI GGNCC 3 cut(s) 4, 610, 1273
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 5 cut(s) 346, 452, 896, 1238, 1280
PvuII CAGCTG 3 cut(s) 887, 1337, 1367
RruI TCGCGA 2 cut(s) 1677, 1789
RsaI GTAC 3 cut(s) 298, 1721, 1765
RsaNI GTAC 3 cut(s) 297, 1720, 1764
RseI CAYNNNNRTG 1 cut(s) 999
SacI GAGCTC 1 cut(s) 1400
SaqAI TTAA 8 cut(s) 117, 333, 555, 615, 1496, 1592, 1598, 1706
SatI GCNGC 4 cut(s) 885, 928, 1132, 1365
Sau96I GGNCC 3 cut(s) 4, 610, 1273
ScrFI CCNGG 6 cut(s) 104, 125, 571, 796, 821, 1644
SduI GDGCHC 3 cut(s) 226, 734, 1400
SfaNI GCATC 7 cut(s) 12, 39, 153, 159, 346, 1336, 1661
SinI GGWCC 2 cut(s) 610, 1273
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 3 cut(s) 82, 332, 1242
SmoI CTYRAG 3 cut(s) 82, 332, 1242
SphI GCATGC 1 cut(s) 698
Sse9I AATT 9 cut(s) 43, 250, 396, 436, 552, 772, 787, 1671, 1817
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 4 cut(s) 350, 374, 1421, 1807
SspMI CTAG 1 cut(s) 894
SstI GAGCTC 1 cut(s) 1400
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 6 cut(s) 102, 123, 569, 794, 819, 1642
StyI CCWWGG 2 cut(s) 269, 317
TaaI ACNGT 7 cut(s) 57, 582, 664, 736, 869, 986, 1473
TaiI ACGT 2 cut(s) 912, 1786
TaqI TCGA 4 cut(s) 47, 180, 988, 1449
TasI AATT 9 cut(s) 43, 250, 396, 436, 552, 772, 787, 1671, 1817
TatI WGTACW 3 cut(s) 296, 1719, 1763
TfiI GAWTC 9 cut(s) 175, 310, 508, 635, 1148, 1298, 1331, 1415, 1436
Tru1I TTAA 8 cut(s) 117, 333, 555, 615, 1496, 1592, 1598, 1706
Tru9I TTAA 8 cut(s) 117, 333, 555, 615, 1496, 1592, 1598, 1706
TscAI CASTG 5 cut(s) 739, 751, 874, 1300, 1528
TseFI GTSAC 3 cut(s) 237, 500, 1751
TseI GCWGC 4 cut(s) 884, 927, 1131, 1364
Tsp45I GTSAC 3 cut(s) 237, 500, 1751
TspDTI ATGAA 8 cut(s) 279, 510, 579, 609, 627, 955, 987, 1683
TspGWI ACGGA 2 cut(s) 198, 692
TspRI CASTG 5 cut(s) 739, 751, 874, 1300, 1528
Van91I CCANNNNNTGG 2 cut(s) 109, 1064
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 610, 1273
XapI RAATTY 7 cut(s) 43, 250, 396, 772, 787, 1671, 1817
XbaI TCTAGA 1 cut(s) 893
XceI RCATGY 3 cut(s) 303, 698, 1235
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 5 cut(s) 512, 775, 994, 1374, 1817
XspI CTAG 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.