AT1G12730

Phosphatidylinositol glycan anchor biosynthesis class U protein-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009567
Physical Location & Seq
Forward (+)
4334499 .. 4337957
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12730.2

Sequence Viewer

Length: 1425 bp
ATGAAGGAGGAGAAGAAAGACGACGGAGTAATGGCTGAGGAGAAGCCTTACATGTTCTGGATTTGGACTTTGGCTTCTACGATTTTCCGATTATTCCTGATCTTTTCCCCGGCAAATATCAACCTATCTTCCCGTCCGGAGGTCTCCACCCCTCACACCAGCCTTCGCCGCCTTGCTGAAGGTTACTGGTTGAAGCAGTCATCACTGTCGCCATACGCAGGATCAATGTATCATGGCTCTCCATTGCTACTCTCCATTCTCGGTCCTCTCACAGTTCAGAGGATCGAAGGGCAATCGAGTCATCATTTGTGCAGTTTGGTTTTTGTTATTGCAGATTTACTTAGTGCACTGCTTCTTCGAGCTATTGGTCAGAAGCTCCAGAAGTCTTATAGAATGAATTTGAGGCCACTTGGTGTTCTTAGGTCATCGCAGGATAGAGGGATCCTTCCTTGTGGAGATATTGCTGCTCTTGTGTATCTGTGGAATCCTTTCACAATACTTTCTTGTGTTGGCTTATCTACTTCTCCAATAGAAAATTTGGCTGTCATTGTGTCTCTTTATGGAGCAGTTACTCGACGAGTTCCTCTGGCTGCATTTGGGTTAATCATGGCCACACATTTGTCTCTTTATTCAGCACCTTTAGTGTTTCCATTGATCTTTCTGTTGGGATATGGTTTAGATGCCCCTCCTATAAAAAAGTTCCTTCAGACAAGACATGGAGGGGTTGAGACTGGATCAACCTCTGATGTTTCTAAACAAGAAAAGTTGACTCCGACTACACAACTTCCTTTCTCATGGAGAACAGTCTTGCATTTCGTATTTTGGGTATTGGTTTGGTCGAGCTATGTGTTGGTTTTATGCAGCTTATCATTGAAACAATATGGTGGGCTTGAGGAGATGTTCAAAAGGACTTATGGTTTCATTCTCAGAATCGAAGATCTGTCACCAAACATTGGTGTCTTTTGGTACTTCTTCGCAGAAGCCTTCGAGTTTTCCAGAAATTATCTCCTGATAATTTTCAACTTATATATTCTCCTTACTTCGATACCATCTTTAATGTTCCGGTTAAAACATCGACCCTGCTTCCTTGCTTTCGCTTATTTAGCCTTCACTTCGATTCTTAAATCTTATCCTTCAGTTGGAGATGCAGCTTTATACTTGAGTTTATGGGCATTGTTTGTCAATGAATTAACAGACATGGAGTACTCGTTCTTTATCTTCTGTGGATATATCGGATTTTCGCTTCTCAGCCCTGTGATGCACAATTTCTGGATATGGAGGGGAACTGGAAATGCAAACTTCTACTTCGGAAATGCTATGGGCTATGCGTGCTTTCAGTTTATGTTTGTGGAGAAGAGTGTAAACGCAATGCTCAACCACGACCAGGCGCTGAAGAAAGCTACTGCAGAAATGACTAAGAACTGA

Protein Analysis

474

Amino Acids

53.7

Weight (kDa)

9.06

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-U PF06728 21 - 357 2e-83 GPI transamidase subunit PIG-U
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12730 AT1G12730 AT1G63110 AT1G63110 AT1G63110
fragaria_vesca FvH4_5g01570 FvH4_5g01570
malus_domestica MD04G1066500.v1.1 MD06G1073700.v1.1
prunus_persica Prupe.5G091900_v2.0.a1
pyrus_communis pycom04g05980 pycom06g07290
rosa_chinensis RchiOBHm_Chr7g0199111
rosa_laevigata RLG00000003814
rosa_multiflora Rmu_sc0006266.1_g000049
rosa_roxburghii Rroxscaffold_3G00257070
rosa_rugosa Rorug07G0052700 Rorug07G0052800
rosa_samantha Rh7BG179700 Rh7DG179200
rosa_wichuraiana Rw7G015460 Rw7G015490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 953
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 1 cut(s) 169
AclWI GGATC 5 cut(s) 229, 290, 436, 449, 742
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 2 cut(s) 397, 535
AcuI CTGAAG 4 cut(s) 198, 689, 1119, 1412
AdeI CACNNNGTG 1 cut(s) 413
AfaI GTAC 2 cut(s) 968, 1205
AfiI CCNNNNNNNGG 5 cut(s) 139, 218, 953, 1139, 1384
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 4 cut(s) 193, 874, 904, 1021
AjnI CCWGG 1 cut(s) 1383
AloI GAACNNNNNNTCC 2 cut(s) 1193, 1225
AluBI AGCT 6 cut(s) 362, 376, 843, 864, 1151, 1400
AluI AGCT 6 cut(s) 362, 376, 843, 864, 1151, 1400
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 4 cut(s) 148, 558, 627, 722
Alw44I GTGCAC 1 cut(s) 345
AlwI GGATC 5 cut(s) 229, 290, 436, 449, 742
AlwNI CAGNNNCTG 1 cut(s) 1390
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 2 cut(s) 404, 609
ApaLI GTGCAC 1 cut(s) 345
ApeKI GCWGC 4 cut(s) 464, 590, 861, 1148
ApoI RAATTY 2 cut(s) 397, 535
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 1390
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 936
AvaII GGWCC 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 349
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 349
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 4 cut(s) 451, 577, 873, 1160
BccI CCATC 1 cut(s) 1057
BciT130I CCWGG 1 cut(s) 1385
BcnI CCSGG 1 cut(s) 110
BcoDI GTCTC 4 cut(s) 148, 558, 627, 722
BfmI CTRYAG 1 cut(s) 1404
BfoI RGCGCY 1 cut(s) 1391
BglII AGATCT 1 cut(s) 937
BisI GCNGC 5 cut(s) 169, 465, 591, 862, 1149
BlsI GCNGC 5 cut(s) 170, 466, 592, 863, 1150
BmcAI AGTACT 1 cut(s) 1205
Bme1390I CCNGG 2 cut(s) 110, 1385
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 2 cut(s) 110, 1385
BmsI GCATC 3 cut(s) 670, 1135, 1248
BpmI CTGGAG 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 911, 1180
BpuMI CCSGG 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 2 cut(s) 136, 1062
BsaXI ACNNNNNCTCC 2 cut(s) 1193, 1223
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 218, 953, 1139, 1384
Bse1I ACTGG 3 cut(s) 191, 736, 1291
Bse3DI GCAATG 2 cut(s) 242, 1374
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 1 cut(s) 1385
BseDI CCNNGG 1 cut(s) 108
BseLI CCNNNNNNNGG 5 cut(s) 139, 218, 953, 1139, 1384
BseMI GCAATG 2 cut(s) 242, 1374
BseMII CTCAG 3 cut(s) 27, 940, 1261
BseNI ACTGG 3 cut(s) 191, 736, 1291
BseRI GAGGAG 3 cut(s) 23, 53, 908
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 4 cut(s) 451, 577, 873, 1160
BsgI GTGCAG 1 cut(s) 331
BshFI GGCC 2 cut(s) 406, 611
BsiHKAI GWGCWC 1 cut(s) 349
BsiSI CCGG 3 cut(s) 110, 137, 1063
BslI CCNNNNNNNGG 5 cut(s) 139, 218, 953, 1139, 1384
BsmAI GTCTC 4 cut(s) 148, 558, 627, 722
BsnI GGCC 2 cut(s) 406, 611
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 349
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
BspACI CCGC 1 cut(s) 169
BspANI GGCC 2 cut(s) 406, 611
BspCNI CTCAG 3 cut(s) 28, 939, 1260
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 443
BspMAI CTGCAG 1 cut(s) 1408
BspPI GGATC 5 cut(s) 229, 290, 436, 449, 742
BspTNI GGTCTC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 242, 1374
BsrI ACTGG 3 cut(s) 191, 736, 1291
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
Bst2UI CCWGG 1 cut(s) 1385
Bst4CI ACNGT 3 cut(s) 207, 274, 805
Bst6I CTCTTC 1 cut(s) 1349
BstC8I GCNNGC 1 cut(s) 1330
BstDEI CTNAG 6 cut(s) 36, 341, 419, 926, 1247, 1416
BstH2I RGCGCY 1 cut(s) 1391
BstHHI GCGC 1 cut(s) 1390
BstKTI GATC 7 cut(s) 102, 224, 285, 444, 657, 737, 940
BstMAI GTCTC 4 cut(s) 148, 558, 627, 722
BstMBI GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
BstMWI GCNNNNNNNGC 3 cut(s) 168, 1103, 1329
BstNI CCWGG 1 cut(s) 1385
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 108, 1383
BstSFI CTRYAG 1 cut(s) 1404
BstSLI GKGCMC 1 cut(s) 349
BstV1I GCAGC 4 cut(s) 451, 577, 873, 1160
BstX2I RGATCY 2 cut(s) 441, 937
BstYI RGATCY 2 cut(s) 441, 937
BsuRI GGCC 2 cut(s) 406, 611
BtgZI GCGATG 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 2 cut(s) 203, 347
Cac8I GCNNGC 1 cut(s) 1330
CaiI CAGNNNCTG 1 cut(s) 1390
CfoI GCGC 1 cut(s) 1390
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 967, 1204
CviAII CATG 6 cut(s) 52, 233, 607, 716, 795, 1198
CviQI GTAC 2 cut(s) 967, 1204
DdeI CTNAG 6 cut(s) 36, 341, 419, 926, 1247, 1416
DpnI GATC 7 cut(s) 101, 223, 284, 443, 656, 736, 939
DpnII GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
DraIII CACNNNGTG 1 cut(s) 413
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 1349
EarI CTCTTC 1 cut(s) 1349
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 263
Eco57I CTGAAG 4 cut(s) 198, 689, 1119, 1412
EcoRII CCWGG 1 cut(s) 1383
FaeI CATG 6 cut(s) 55, 236, 610, 719, 798, 1201
FatI CATG 6 cut(s) 51, 232, 606, 715, 794, 1197
Fnu4HI GCNGC 5 cut(s) 169, 465, 591, 862, 1149
Fsp4HI GCNGC 5 cut(s) 169, 465, 591, 862, 1149
GlaI GCGC 1 cut(s) 1389
GluI GCNGC 5 cut(s) 169, 465, 591, 862, 1149
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 1391
HaeIII GGCC 2 cut(s) 406, 611
HapII CCGG 3 cut(s) 110, 137, 1063
HhaI GCGC 1 cut(s) 1390
Hin1II CATG 6 cut(s) 55, 236, 610, 719, 798, 1201
Hin6I GCGC 1 cut(s) 1388
HinP1I GCGC 1 cut(s) 1388
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HinfI GANTC 5 cut(s) 298, 484, 769, 930, 1117
HpaII CCGG 3 cut(s) 110, 137, 1063
HphI GGTGA 1 cut(s) 936
Hpy166II GTNNAC 3 cut(s) 347, 768, 1363
Hpy188I TCNGA 9 cut(s) 89, 279, 372, 708, 745, 774, 929, 1235, 1310
Hpy188III TCNNGA 7 cut(s) 58, 97, 137, 379, 996, 1009, 1270
Hpy8I GTNNAC 3 cut(s) 347, 768, 1363
Hpy99I CGWCG 2 cut(s) 26, 579
HpyAV CCTTC 8 cut(s) 173, 173, 281, 455, 713, 994, 1117, 1143
HpyCH4III ACNGT 3 cut(s) 207, 274, 805
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 1103, 1329
HpyF3I CTNAG 6 cut(s) 36, 341, 419, 926, 1247, 1416
Hsp92II CATG 6 cut(s) 55, 236, 610, 719, 798, 1201
HspAI GCGC 1 cut(s) 1388
Kpn2I TCCGGA 1 cut(s) 136
Kzo9I GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
LmnI GCTCC 2 cut(s) 381, 563
Lsp1109I GCAGC 4 cut(s) 451, 577, 873, 1160
LweI GCATC 3 cut(s) 670, 1135, 1248
MaeIII GTNAC 3 cut(s) 182, 568, 942
MalI GATC 7 cut(s) 101, 223, 284, 443, 656, 736, 939
MboI GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
MboII GAAGA 8 cut(s) 25, 120, 347, 947, 964, 1210, 1366, 1405
MflI RGATCY 2 cut(s) 441, 937
MhlI GDGCHC 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 611
MluCI AATT 6 cut(s) 397, 535, 1000, 1014, 1187, 1264
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 2 cut(s) 307, 763
MmeI TCCRAC 2 cut(s) 797, 1120
Mox20I TGGCCA 1 cut(s) 611
MroI TCCGGA 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 488
MscI TGGCCA 1 cut(s) 611
MseI TTAA 5 cut(s) 602, 1055, 1067, 1122, 1190
Msp20I TGGCCA 1 cut(s) 611
MspI CCGG 3 cut(s) 110, 137, 1063
MspR9I CCNGG 2 cut(s) 110, 1385
MvaI CCWGG 1 cut(s) 1385
MwoI GCNNNNNNNGC 3 cut(s) 168, 1103, 1329
NciI CCSGG 1 cut(s) 110
NdeII GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
NlaIII CATG 6 cut(s) 55, 236, 610, 719, 798, 1201
NlaIV GGNNCC 1 cut(s) 443
NmuCI GTSAC 1 cut(s) 942
NspI RCATGY 1 cut(s) 55
PciI ACATGT 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 3 cut(s) 484, 930, 1117
PflMI CCANNNNNTGG 1 cut(s) 953
PkrI GCNGC 5 cut(s) 170, 466, 592, 863, 1150
PleI GAGTC 2 cut(s) 306, 763
PpsI GAGTC 2 cut(s) 306, 763
PscI ACATGT 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 1383
PspGI CCWGG 1 cut(s) 1383
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 1 cut(s) 263
PstI CTGCAG 1 cut(s) 1408
PstNI CAGNNNCTG 1 cut(s) 1390
PsuI RGATCY 2 cut(s) 441, 937
RsaI GTAC 2 cut(s) 968, 1205
RsaNI GTAC 2 cut(s) 967, 1204
SaqAI TTAA 5 cut(s) 602, 1055, 1067, 1122, 1190
SatI GCNGC 5 cut(s) 169, 465, 591, 862, 1149
Sau3AI GATC 7 cut(s) 99, 221, 282, 441, 654, 734, 937
Sau96I GGNCC 1 cut(s) 263
ScaI AGTACT 1 cut(s) 1205
SchI GAGTC 2 cut(s) 307, 763
ScrFI CCNGG 2 cut(s) 110, 1385
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 3 cut(s) 670, 1135, 1248
SfcI CTRYAG 1 cut(s) 1404
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 2 cut(s) 890, 1159
SmoI CTYRAG 2 cut(s) 890, 1159
Sse9I AATT 6 cut(s) 397, 535, 1000, 1014, 1187, 1264
SsiI CCGC 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 108, 1383
TaaI ACNGT 3 cut(s) 207, 274, 805
TaqII GACCGA 1 cut(s) 251
TasI AATT 6 cut(s) 397, 535, 1000, 1014, 1187, 1264
TatI WGTACW 1 cut(s) 1203
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 3 cut(s) 484, 930, 1117
Tru1I TTAA 5 cut(s) 602, 1055, 1067, 1122, 1190
Tru9I TTAA 5 cut(s) 602, 1055, 1067, 1122, 1190
TscAI CASTG 2 cut(s) 210, 354
TseFI GTSAC 1 cut(s) 942
TseI GCWGC 4 cut(s) 464, 590, 861, 1148
Tsp45I GTSAC 1 cut(s) 942
TspDTI ATGAA 4 cut(s) 17, 410, 910, 1200
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 2 cut(s) 210, 354
Van91I CCANNNNNTGG 1 cut(s) 953
VneI GTGCAC 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 2 cut(s) 397, 535
XceI RCATGY 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 488
ZrmI AGTACT 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.